by Chu Kin Kan Astley. / Thesis submitted in: December 2000. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2001. / Includes bibliographical references (leaves xi-xx (3rd gp.)). / Abstracts in English and Chinese. / Abstract (English) --- p.i / Abstract (Chinese) --- p.iii / Acknowledgments --- p.iv / Table of Contents --- p.v / List of Tables --- p.ix / List of Figures --- p.x / Chapter Chapter 1 --- General Introduction / Chapter 1.1 --- Popularity of Shiitake Mushroom --- p.1 / Chapter 1.2 --- Inheritance of Genetic Materials in L.edodes --- p.1 / Chapter 1.3 --- Genetic Markers and Linkage Maps of L.edodes --- p.2 / Chapter 1.4 --- Aims of Study --- p.5 / Chapter Chapter 2 --- Mapping of Sequence Tagged Sites (STSs) and Expressed Sequence Tags (ESTs) on the Linkage Map of L.edodes by PCR-Single Strand Conformational Polymorphism (SSCP) Test / Chapter 2.1 --- Literature Review --- p.7 / Chapter 2.1.1 --- Construction of Genetic Linkage Map --- p.7 / Chapter 2.1.2 --- Logarithm of the Odds (LOD) Score --- p.8 / Chapter 2.1.3 --- MAPMAKER Program --- p.10 / Chapter 2.1.4 --- Sequence Tagged Site (STS) and Expressed Sequence Tag (EST) --- p.11 / Chapter 2.1.5 --- Polymerase Chain Reaction - Single Strand Conformational Polymorphism (PCR-SSCP) --- p.13 / Chapter 2.2 --- Material and Methods --- p.18 / Chapter 2.2.1. --- Detection of the STS and EST markers with PCR-SSCP test --- p.18 / Chapter 2.2.1.1 --- Biological Material and Growth Conditions --- p.18 / Chapter 2.2.1.2 --- DNA Samples Preparation --- p.18 / Chapter 2.2.1.3 --- PCR Primers Designation --- p.20 / Chapter 2.2.1.4 --- PCR Amplification --- p.21 / Chapter 2.2.1.5 --- SSCP Test --- p.21 / Chapter 2.2.1.6 --- Silver Staining of the Polyacrylamide Gel --- p.22 / Chapter 2.2.2. --- Mapping of the STS and EST Markers --- p.22 / Chapter 2.2.2.1 --- Biological Material and Growth Conditions --- p.22 / Chapter 2.2.2.2 --- SSI DNA Preparation --- p.22 / Chapter 2.2.2.3 --- PCR-SSCP Test among SSIs --- p.24 / Chapter 2.2.2.4 --- Chi Square (X2) Test --- p.24 / Chapter 2.2.2.5 --- LOD Score Test and Mapping of the Markers --- p.26 / Chapter 2.3 --- Results --- p.27 / Chapter 2.3.1 --- Detection of the STS and EST Markers from PCR-SSCP Test --- p.27 / Chapter 2.3.1.1 --- DNA Sample Preparation --- p.27 / Chapter 2.3.1.2 --- Primers Designed for STS and EST Amplification --- p.27 / Chapter 2.3.1.3 --- Size Differences between Experimental and Expected PCR Products --- p.38 / Chapter 2.3.1.4 --- Markers of PCR Polymorphism (PCRP) --- p.38 / Chapter 2.3.1.5 --- Markers of PCR-SSCP and PCR Length Polymorphism (PCR-LP) --- p.42 / Chapter 2.3.2 --- Mapping of the STS/EST Markers --- p.49 / Chapter 2.3.2.1 --- DNA Templates of SSIs --- p.49 / Chapter 2.3.2.2 --- Polymorphism Profiles of STSs and ESTs among the L54-SSIs --- p.49 / Chapter 2.3.2.3 --- Chi-square Test --- p.55 / Chapter 2.3.2.4 --- Repeated EST Markers --- p.58 / Chapter 2.3.2.5 --- Anonymous Expressed Sequence Tag - EST31 --- p.58 / Chapter 2.3.2.6 --- Linkage Analysis and Mapping of Markers --- p.59 / Chapter 2.3.2.6.1 --- Linkage Relationships between the16 STS/EST Markers --- p.59 / Chapter 2.3.2.6.2 --- Mapping of the STS/EST Markers onto the RAPD Linkage Map --- p.62 / Chapter 2.4 --- Discussion --- p.69 / Chapter 2.4.1 --- DNA Template Preparation --- p.69 / Chapter 2.4.2 --- Size Difference Between Expected and Experimental PCR Product --- p.69 / Chapter 2.4.3 --- PCR Polymorphism (PCRP) --- p.70 / Chapter 2.4.4 --- PCR-LP --- p.70 / Chapter 2.4.5 --- PCR-SSCP --- p.71 / Chapter 2.4.5.1 --- Primer Designed for PCR-SSCP --- p.71 / Chapter 2.4.5.2 --- Markers Producing Efficiency of PCR-SSCP Test --- p.72 / Chapter 2.4.6 --- Linkage Map of L.edodes --- p.73 / Chapter 2.4.6.1 --- Map Distance --- p.73 / Chapter 2.4.6.2 --- Linkage Groups --- p.74 / Chapter 2.4.6.3 --- Map Markers --- p.75 / Chapter Chapter 3 --- Mapping of Agronomic Features of L.edodes / Chapter 3.1 --- Literature Review --- p.77 / Chapter 3.1.1 --- Aroma Feature of L.edodes --- p.77 / Chapter 3.1.1.1 --- Volatile Compounds in Shiitake (L.edodes) Mushroom --- p.77 / Chapter 3.1.1.2 --- Fragrance Signature of Shiitake Mycelium --- p.79 / Chapter 3.1.2 --- Mapping of Quantitative Trait Loci (QTL) --- p.84 / Chapter 3.1.2.1 --- Complex Traits --- p.84 / Chapter 3.1.2.2 --- Quantitative Traits Locus (QTL) --- p.85 / Chapter 3.1.2.3 --- Maximum-likelihood Estimate in QTL mapping --- p.86 / Chapter 3.1.2.4 --- MAPMAKER/QTL --- p.87 / Chapter 3.2 --- Material and Methods --- p.88 / Chapter 3.2.1 --- Aroma feature of Mycelium --- p.88 / Chapter 3.2.1.1 --- Preliminary Screening of Volatiles in the SSI Mycelia of L.edodes --- p.88 / Chapter 3.2.1.1.1 --- Biological Material and Growth Conditions --- p.88 / Chapter 3.2.1.1.2 --- Volatile Extraction from SSI Mycelia --- p.88 / Chapter 3.2.1.1.3 --- Screening of Volatile Compounds with GC-MS --- p.89 / Chapter 3.2.1.2 --- Quantification of the Target Aromatic Volatile in the Mycelia of SSI and Parents --- p.90 / Chapter 3.2.1.2.1 --- Sample Preparations --- p.90 / Chapter 3.2.1.2.2 --- Quantification of the Target Volatile --- p.90 / Chapter 3.2.2 --- Measurement of Mycelial Growth --- p.91 / Chapter 3.2.3 --- Observation of Pigment Secretion during Mycelial Growth --- p.91 / Chapter 3.2.4 --- Locating Putative QTL on the Genetic Map of L.edodes --- p.92 / Chapter 3.3 --- Results --- p.93 / Chapter 3.3.1 --- Aroma Feature --- p.93 / Chapter 3.3.1.1 --- Preliminary Screening of Volatiles in Mycelia of L.edodes --- p.93 / Chapter 3.3.1.2 --- Quantification of l-octen-3-ol in SSI Mycelia --- p.103 / Chapter 3.3.1.2.1 --- Sample Preparation --- p.103 / Chapter 3.3.1.2.2 --- l-Octen-3-ol contents in SSI Mycelia --- p.103 / Chapter 3.3.1.3 --- Mapping of QTL for l-octen-3-ol level on the genetic map --- p.106 / Chapter 3.3.2 --- Mycelial Growth Rate (MGR) --- p.111 / Chapter 3.3.2.1 --- Measurement of Mycelial Growth Rate --- p.111 / Chapter 3.3.2.2 --- Mapping of QTL for MGR on the genetic map --- p.111 / Chapter 3.3.3 --- Pigment Secretion form SSI mycelia --- p.116 / Chapter 3.4 --- Discussion --- p.118 / Chapter 3.4.1 --- Significance of the QTLs --- p.118 / Chapter 3.4.2 --- QTL for Aroma Feature --- p.119 / Chapter 3.4.2.1 --- Trait of Aroma: l-octen-3-ol level --- p.119 / Chapter 3.4.2.2 --- QTL of l-octen-3-ol level --- p.120 / Chapter 3.4.3 --- Mycelial Growth Rate (MGR) --- p.123 / Chapter 3.4.4 --- Pigment Secretion --- p.125 / Chapter Chapter 4 --- General Discussion and Conclusions --- p.127 / Chapter 4.1 --- Future Works --- p.127 / Chapter 4.1.1 --- Mapping of L.edodes Genes --- p.127 / Chapter 4.1.2 --- Characterizing and Mapping of Agronomic Traits --- p.128 / Chapter 4.2 --- Conclusions --- p.128 / Referencesxi
| Identifer | oai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_323408 |
| Date | January 2001 |
| Contributors | Chu, Kin Kan Astley., Chinese University of Hong Kong Graduate School. Division of Food and Nutritional Sciences. |
| Source Sets | The Chinese University of Hong Kong |
| Language | English, Chinese |
| Detected Language | English |
| Type | Text, bibliography |
| Format | print, x, 131, xi-xx leaves : ill. ; 30 cm. |
| Rights | Use of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/) |
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