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Improved algorithm for entropic segmentation of DNA sequence /Wang, Zhenggang. January 2004 (has links)
Thesis (M. Phil.)--Hong Kong University of Science and Technology, 2004. / Includes bibliographical references (leaves 56-58). Also available in electronic version. Access restricted to campus users.
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Development and application of methods for targeted DNA sequencing of pooled samplesMusgrave-Brown, Esther January 2014 (has links)
No description available.
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Further development of the visual genome explorer a visual genomic comparative tool /Cheng, Kai-hong. January 2001 (has links)
Thesis (M. Phil.)--University of Hong Kong, 2001. / Includes bibliographical references (leaves 112-114).
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A computational estimation of errors in model genomes using exactly duplicated DNA sequences /Siu, Kim-Man. January 2005 (has links)
Thesis (M.Phil.)--Hong Kong University of Science and Technology, 2005. / Includes bibliographical references (leaves 41-43). Also available in electronic version.
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UCTDVan Der Merwe, Pieter de Wet 04 1900 (has links)
Thesis (MSc)--Stellenbosch University, 2015. / ENGLISH ABSTRACT: Zygophyllum orbiculatum Welwitsch ex Oliv. from Angola and Zygophyllum stapffii Schinz
from Namibia were described in the late 1800’s. Recent comparisons of these two species
revealed that they were morphologically very similar except that Zygophyllum orbiculatum has
unifoliolate leaves and Zygophyllum stapffii has bifoliolate leaves. The similarity of these two
species was investigated using nuclear ITS (Internal Transcribed Spacer, i.e. ITS1, 5.8SrDNA,
ITS2) region sequence data as phylogenetic markers. Due to almost identical sequences and
phylogenetic grouping, it was concluded that they were conspecific. However, the phylogenetic
relationships of the major groups within the subfamily Zygophylloideae based on ITS sequences,
were unresolved and unsupported, as was found in previous studies using chloroplast gene
marker sequences.
To resolve the phylogenetic relationships of the major groups within the subfamily
Zygophylloideae, a next generation sequencing (NGS) approach was taken. Chloroplasts of taxa
representing the major groups within the subfamily were isolated and chloroplast genome
sequence data were generated using the Ion Torrent™ sequencer. Additional nuclear ITS
cassette data (18SrDNA, ITS1, 5.8SrDNA, ITS2, 26SrDNA) were generated as a by-product and
used to produce a large combined aligned sequence matrix for phylogenetic analysis.
Model-based phylogenetic programs were able to retrieve strongly supported and resolved
phylogenetic relationships of the major groups within Zygophylloideae. Two basal groupings
were retrieved in the subfamily. The first grouping consisted of the genera Tetraena, Fagonia
and Melocarpum. The second grouping consisted of the monotypic genus Augea and
Zygophyllum orbiculatum/stapffii which were embedded within the genus Roepera. Using a gene
duplication approach, the chloroplast marker data of genus Zygophyllum sensu stricto placed this
genus basal to the Augea, Zygophyllum orbiculatum/stapffii, Roepera clade whilst the nuclear
marker data of Zygophyllum sensu stricto, was found in a basal position to the entire subfamily. From this it is concluded that Zygophyllum sensu stricto shows evidence of incomplete lineage
sorting. A revised taxonomy for the entire subfamily Zygophylloideae is proposed. / AFRIKAANSE OPSOMMING: Zygophyllum orbiculatum Welwitsch ex Oliv. uit Angola en Zygophyllum stapffii Schinz van
Namibië is in die laat 1800's beskryf. Onlangse vergelykings van hierdie twee spesies het getoon
dat hulle morfologies baie eners is, behalwe dat Zygophyllum orbiculatum unifoliolate blare
besit en dat Zygophyllum stapffii bifoliolate blare besit. Hierdie ooreenkoms is ondersoek, met
behulp van die nukleêre “ITS” (Internal Transcribed Spacer d.w.s. ITS1, 5.8SrDNA, ITS2)
DNS-strook volgordedata as filogenetiese merkers. As gevolg van feitlik identiese
geenopeenvolgings is bevind dat die twee spesies konspesifiek is. Die filogenetiese
verwantskappe van die groot binnegroepe van die subfamilie Zygophylloideae, gebaseer op ITS
geenopeenvolgings, was egter onopgelos en nie ondersteun nie, net soos in vorige studies waarin
chloroplast geenmerkervolgordes gebruik was.
Om die filogenetiese verwantskappe van die groot binnegroepe van die subfamilie
Zygophylloideae op te los, was ‘n betreklik nuwe DNS volgordebepalingstegniek, naamlik
“Next Generation Sequencing” (NGS), gebruik. Chloroplaste van taksa, wat die groot groepe
binne-in die subfamilie verteenwoordig, is geïsoleer en chloroplast genoomdata is gegenereer
met behulp van die Ion Torrent ™ (NGS) DNS-volgordebepaler. Bykomend was die nukleêre
“ITS”-kasset volgordedata (18SrDNS, ITS1, 5.8SrDNS, ITS2, 26SrDNS) ook as 'n by-produk
gegenereer en ook gebruik om 'n groot gesamentlike DNS oplyningmatriks vir filogenetiese
doeleindes.
Model-gebaseerde filogenetiese programme was in staat was om sterk ondersteuning en
opgeloste filogenetiese verwantskappe van die groot groepe binne-in Zygophylloideae te
ontravel. Die subfamilier toon twee basale groeperinge. Die eerste groepering bestaan uit die
genera Tetraena, Fagonia en Melocarpum. Die tweede groepering bestaan uit die monotipiese
genus Augea en Zygophyllum orbiculatum/stapffii, wat ingebed is binne-in die genus Roepera.
Deur ‘n geendupliseringsbenadering te gebruik op die DNS geenopeenvolgings van die
verteenwoordigende takson van Zygophyllum sensu stricto, is bevind dat die chloroplast DNS
volgordes hierdie groep basaal aan ‘n Roepera/Augea/Zygophyllum orbiculatum/stapffii klade
plaas, terwyl die nukleêre DNS volgordes hierdie groep basaal aan die hele subfamilie
Zygophylloideae plaas. Hieruit is die gevolgtrekking gemaak dat Zygophyllum sensu scricto
bewyse van onvolledige afstammelingsortering toon. ‘n Gewysigde taksonomie vir die hele
subfamilie Zygophylloideae word voorgestel.
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Application of 16S rRNA gene sequencing in laboratory diagnosis of mycobacteria other than tuberculosis金倩儀, Kam, Sin-yee. January 2003 (has links)
published_or_final_version / Medical Sciences / Master / Master of Medical Sciences
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LOCALIZATION AND COMPLETE NUCLEOTIDE SEQUENCE OF THE BOVINE PAPILLOMAVIRUS TYPE-2 LATE REGION. POLYPEPTIDE COMPOSITION OF BPV-2.POTTER, HAROLD LEE, JR. January 1984 (has links)
This investigation was designed to partially characterize the genome of bovine papillomavirus type-2 (BPV-2). A closely related virus, bovine papillomavirus type-1 (BPV-1), has been extensively studied. Additionally, many members of the human papillomaviruses (HPV) are being investigated in great detail. However, only a few molecular biology studies of BPV-2 have been reported. Therefore, it seemed appropriate to initiate an investigation whose results would provide a good basis for continued studies of BPV-2. A detailed physical map of BPV-2 DNA was prepared by restriction endonuclease digestion of BPV-2 DNA. A comparison between the genomes of BPV-2 and BPV-1 in regard to location and number of cleavage sites of seven enzymes was performed. This comparison revealed similarities between the two genomes and indicated the probable location of the BPV-2 late region by direct extrapolation from the BPV-1 genomic map. This area was completely sequenced by the chemical method and the results confirmed it to indeed be the BPV-2 late region. Comparative analysis between the BPV-2 late region and the BPV-1 late region demonstrated identical genetic organization to exist along with a high degree of nucleotide sequence conservation. Also, the polypeptide composition of BPV-2 was determined and similarities with these and BPV-1 polypeptides were noted. Lastly, a discussion of the BPV-2 polypeptides as potential products of the BPV-2 late region is presented.
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Motif discovery for DNA sequencesLeung, Chi-ming, January 2006 (has links)
Thesis (Ph. D.)--University of Hong Kong, 2007. / Title proper from title frame. Also available in printed format.
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DFT based splicing algorithms for prediction of protein coding regions /Wang, Haoyuan. January 2004 (has links)
Thesis (M.Sc.)--York University, 2004. Graduate Programme in Computer Science. / Typescript. Includes bibliographical references (leaves 98-100). Also available on the Internet. MODE OF ACCESS via web browser by entering the following URL:http://gateway.proquest.com/openurl?url%5Fver=Z39.88-2004&res%5Fdat=xri:pqdiss&rft%5Fval%5Ffmt=info:ofi/fmt:kev:mtx:dissertation&rft%5Fdat=xri:pqdiss:MQ99401
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Nucleotide sequence of cDNA of bone-mineralizing hormone calcitonin in medaka (Teleostei)Sakamoto, Hidenori, Sasayama, Yuichi January 2007 (has links)
No description available.
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