Li Pik Ha Ivy. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2006. / Includes bibliographical references (leaves 83-87). / Abstracts in English and Chinese. / TABLE OF CONTENTS --- p.i / LIST OF FIGURES --- p.v / LIST OF TABLES --- p.vii / ABSTRACT --- p.viii / ACKNOWLEDGEMENT --- p.x / DECLARATION --- p.xi / ABBREVIATIONS --- p.xii / Chapter Chapter one --- Introduction / Chapter 1.1 --- Background --- p.1 / Chapter 1.2 --- Polysaccharides isolated from Ganoderma Lucidum --- p.4 / Chapter 1.3 --- Conventional methods for molecular weight (MW) determination of polysaccharides --- p.6 / Chapter 1.3.1 --- Osmometry --- p.7 / Chapter 1.3.2 --- Light Scattering --- p.8 / Chapter 1.3.3 --- Intrinsic Viscosity --- p.8 / Chapter 1.3.4 --- Size Exclusion Chromatography (SEC) --- p.9 / Chapter 1.3.5 --- Mass Spectrometry --- p.10 / Chapter 1.4 --- Matrix-assisted Laser Desorption / Ionization Mass Spectrometry --- p.12 / Chapter 1.5 --- MALDI-TOF Mass Spectrometry of polysaccharides --- p.13 / Chapter 1.6 --- Outline of project --- p.15 / Chapter Chapter two --- Instrumental and experimental / Chapter 2.1 --- Instrumentation --- p.18 / Chapter 2.1.1 --- Laser-based ion source --- p.18 / Chapter 2.1.2 --- Time-of-flight (TOF) analyzer --- p.19 / Chapter 2.1.3 --- Ion deflector --- p.23 / Chapter 2.1.4 --- Detector and data acquisition system --- p.23 / Chapter 2.2 --- Experimental --- p.26 / Chapter 2.2.1 --- Isolation of water-soluble polysaccharides from Ganoderma Lucidum by water extraction --- p.26 / Chapter 2.2.2 --- Isolation of water-soluble polysaccharides from Ganoderma Lucidum by dimethyl ssulfoxide (DMSO) extraction --- p.26 / Chapter 2.2.3 --- Fractionation of water-soluble polysaccharides by Gel Permeation Chromatography (GPC) --- p.27 / Chapter 2.2.4 --- Bradford protein assay --- p.28 / Chapter 2.2.5 --- Phenol / sulfuric acid assay --- p.28 / Chapter 2.2.6 --- Sample preparation in MS --- p.28 / Chapter 2.2.7 --- Calibration of MALDI-TOF-MS --- p.29 / Chapter 2.2.8 --- Data analysis --- p.29 / Chapter Chapter three --- Extraction and purification of water-soluble polysaccharides from Ganoderma Lucidum / Chapter 3.1 --- Introduction --- p.30 / Chapter 3.2 --- Experimental --- p.32 / Chapter 3.2.1 --- Extraction efficiency --- p.32 / Chapter 3.2.2 --- Dialysis --- p.32 / Chapter 3.2.3 --- Signal suppression effect --- p.32 / Chapter 3.2.4 --- Sevag method --- p.33 / Chapter 3.2.5 --- Trichloroacetic acid (TCA) precipitation --- p.33 / Chapter 3.2.6 --- Bradford Protein Assay --- p.34 / Chapter 3.2.7 --- Phenol sulfuric acid assay --- p.34 / Chapter 3.3 --- Results and discussion --- p.35 / Chapter 3.3.1 --- Extraction efficiency --- p.35 / Chapter 3.3.2 --- Purification of crude polysaccharides --- p.37 / Chapter 3.3.3 --- Desalting --- p.38 / Chapter 3.3.4 --- Deproteination --- p.40 / Chapter 3.3.4.1 --- Monitoring of protein contents --- p.40 / Chapter 3.3.4.2 --- Monitoring of carbohydrate contents --- p.45 / Chapter 3.3.4.3 --- Deproteination using Sevag and TCA procipitation method --- p.47 / Chapter 3.4 --- Conclusions --- p.54 / Chapter Chapter four --- Evaluation of MW and MWD of water-soluble Polysaccharides extracted from Ganoderma Lucidum / Chapter 4.1 --- Introduction --- p.55 / Chapter 4.2 --- Experimental --- p.58 / Chapter 4.2.1 --- Aqueous DHB matrix --- p.58 / Chapter 4.2.2 --- Aqueous DHB/NH4F matrix --- p.58 / Chapter 4.2.3 --- Aqueous DHB matrix in TA solution --- p.58 / Chapter 4.2.4 --- Aqueous DHB/NH4F matrix in TA solution --- p.59 / Chapter 4.2.5 --- Aqueous DHB/NH4F matrix with sodium salt in TA solution --- p.59 / Chapter 4.2.6 --- Aqueous DHB/NH4F matrix with potassium salt in TA solution --- p.59 / Chapter 4.2.7 --- Fractionation of water-soluble polysaccharide extracted by DMSO by Gel Permeation Chromatography (GPC) --- p.59 / Chapter 4.2.8 --- Ultra-violet absorption spectrometry (UV-VIS) --- p.60 / Chapter 4.3 --- Results and discussion --- p.60 / Chapter 4.3.1 --- Development of matrix and solvent system for water-soluble polysaccharides --- p.60 / Chapter 4.3.2 --- MW and MWD of water-soluble polysaccharides extracted from Ganoderma Lucidum --- p.64 / Chapter 4.3.2.1 --- Water extraction --- p.65 / Chapter 4.3.2.2 --- DMSO extraction followed by water-back extraction --- p.70 / Chapter 4.4 --- Conclusions --- p.79 / Chapter Chapter five --- Concluding remarks --- p.82 / References --- p.84
| Identifer | oai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_325580 |
| Date | January 2006 |
| Contributors | Li, Pik Ha Ivy., Chinese University of Hong Kong Graduate School. Division of Chemistry. |
| Source Sets | The Chinese University of Hong Kong |
| Language | English, Chinese |
| Detected Language | English |
| Type | Text, bibliography |
| Format | print, xii, 87 leaves : ill. ; 30 cm. |
| Rights | Use of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/) |
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