Urginea sanguinea, Aloe marlothii, Elephantorrhiza elephantina and Rhoicissus tridentate are all plants utilized for the management of tick borne diseases in the Madikwe area of North-west province. These plants, in certain concoctions, are believed to be effective against “seme”, “gala” and “Bolwetsi jwa mothlapo o moshibidu” which we have assumed to represent heartwater, gallsickness and redwater from circumstantial epidemiological data available. To obtain a representative extract, which would be indicative of the general activity of the plant, only acetone or methanol extracts were tested for the presence of antimicrobial, antiparasitic or anti-oxidant activity within that specific plant. Activity in all cases made use of either an in vitro biological assay or more specific chemical tests, which were validated in all cases. Ehrlichia ruminantium, Babesia caballi and Theileria equi, all grown in specific cell cultures, were used as a model for evaluating the efficacy against the common protozoan and rickettsial diseases caused by these organisms in livestock. Staphylococcus aureus, Enterococcus faecalis, Pseudomonas aeruginosa and Escherichia coli, four human nosocomial infectious agents, were used as an indicator for the presence of antibacterial activity against these common animal bacterial pathogens. Diphenyl-picrylhydrazyl and the trolox equivalent anti-oxidant chemical assays were used to determine anti-oxidant activity, which although not curative, may aid in the recovery from an infection by stimulating the immune system. The activities demonstrated among the various plants and organisms were not consistent. E. elephantine extracts were the most effective, with activity demonstrable in all biological and chemical screening assays. Although R. tridentate demonstrated poor activity (> 100 ìg/ml) against the tick-borne parasites, the plant extract did demonstrate significant anti-oxidant activity. U. sanguinea extracts showed good activity in both the antibacterial and anti-rickettsial assays (EC50 = 44.49 ng/ml), which may be due to the presence of the toxic bufadienolides present within the plant. A. marlothii possessed significant anti-rickettsial activity (EC50= 111.4 µg/ml) and to a lesser degree antibacterial activity. The results of the study support the use of these plants against heartwater, gallsickness and redwater, which gives credence for the traditional use against “Seme, Gala, and Bolwetsi jwa mothlapo o moshibidu”. Further studies are required to isolate and determine the structure of the active compounds of these plants as well as to confirm the safety and efficacy of the extracts against disease conditions in livestock. Copyright / Urginea sanguinea, Aloe marlothii, Elephantorrhiza elephantina and Rhoicissus tridentata
word tradisioneel gebruik vir die bekamping van siektes deur bosluise oorgedra in die
Madikwe gebied van die Noordwes provinsie. Ekstrakte van hierdie species word gebruik
teen “seme”, “gala” en “Bolwetsi jwa mothlapo o moshibidu” wat waarskynlik op
hartwater, galsiekte and rooiwater dui volgens die beskikbare epidemiologiese data.
Asetoon en metanol ekstrakte is gebruik vir die bepaling van antimikrobiese,
antiparasitiese en antioksidant aktiwiteite in verskillende species deur gevalideerde in vitro
metodes.
Selkulture van Ehrlichia ruminantium, Babesia caballi en Theileria equi, , is in ‘n model
gebruik om die doeltreffendheid van ekstrakte teen algemene siektes deur protozoa en
ricketsias te bepaal. Vier algemene menslike nosokomiale patogene Staphylococcus
aureus, Enterococcus faecalis, Pseudomonas aeruginosa and Escherichia coli, is gebruik
om antibakteriese aktiwiteit van ekstrakte te bepaal.
Difeniel-pikrielhidrasiel en die trolox ekwivalente anti-oksidant essajeermetode is gebruik
om anti-oksidantaktiwiteit te bepaal. Antioksidante mag herstel na infeksies bespoedig
deur stimulering van die immuunstelsel.
Daar was ‘n groot verskil in die aktiwiteite tussen die verskillende ekstrakte en
organismes. E. elephantina ekstrakte was die mees doeltreffende met die biologiese and
chemiese bepalings. R. tridentata het sterk anti-oksidantaktiwiteit gehad, maar het lae
aktiwiteit (> 100 µg/ml) teen bosluis-oorgedraagde parasiete gehad. U. sanguinea
ekstrakte was aktief in beide die antibakteriese en anti-riketsiale bepalings (EC50 = 44.49
ng/ml), wat moontlik toegeskryf kan word aan die giftige bufadienoliede teenwoordig in
hierdie species. A. marlothii ekstrakte het betekenisbolle anti-riketsiale aktiwiteit (EC50 =
111.4 µg/ml) maar slegs geringe antibakteriese aktiwiteit gehad.
Hierdie resultate bevestig die moontlike waarde van hierdie species teen hartwater,
galsiekte and rooiwater, en ondersteun die tradisionele etnoveterinêre gebruik teen “Seme,
Gala, and Bolwetsi jwa mothlapo o moshibidu”. Verdere studies word benodig om die
5 aktiewe verbindings te isoleer en te karakteriseer en om die veiligheid en doeltreffendheid
van ekstrakte teen hierdie siektes in vee te bevestig. / Dissertation (MSc (Veterinary Science))--University of Pretoria, 2004. / Paraclinical Sciences / unrestricted
| Identifer | oai:union.ndltd.org:netd.ac.za/oai:union.ndltd.org:up/oai:repository.up.ac.za:2263/23002 |
| Date | 08 March 2005 |
| Creators | Naidoo, Vinasan |
| Contributors | Swan, Gerry E., Eloff, Jacobus Nicolaas, upetd@ais.up.ac.za |
| Publisher | University of Pretoria |
| Source Sets | South African National ETD Portal |
| Detected Language | English |
| Type | Dissertation |
| Rights | © 2004, University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretori |
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