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Kinetic studies of Cr(VI) reduction in an indigenous mixed culture of bacteria in the presence of As(III)

An indigenous mixed culture of bacteria collected from a Wastewater Treatment Plant (Brits,
North West Province, South Africa), biocatalytically reduced Cr(VI) in the presence of
As(III). Both the reduced chromium (Cr(III)) and the oxidised arsenic (As(V)) readily form
amorphous hydroxides that can be easily separated or precipitated from the aqueous phase as
part of the treatment process. Treatment of Cr(VI) and As(III) before disposal of wastewater
is critical since both compounds are known to be carcinogenic and mutagenic at very low
concentrations, and acutely toxic at high concentrations.
Batch experiments were conducted to evaluate the rate of Cr(VI) reduction under anaerobic
condition in the presence of its co-contaminant As(III) typically found in the groundwater
and mining effluent. Results showed near complete Cr(VI) reduction under initial Cr(VI)
concentrations up to 70 mg/L in a batch amended with 20 mg/L As(III). However, increasing
Cr(VI) concentrations up to 100 mg/L resulted in the inhibition of Cr(VI) reduction activity.
Further investigation was conducted in a batch reactor amended with 70 mg/L Cr(VI)
concentration at different As(III) concentrations ranging from 5-70 mg/L to evaluate the
effect of varying As(III) concentration on Cr(VI) reduction efficiency. Results showed that
Cr(VI) reduction efficiency increased as As(III) concentrations increased from 5-40 mg/L.
However, further increase in As(III) concentration up to 50 mg/L resulted in incomplete
Cr(VI) reduction and decrease in Cr(VI) reduction efficiency. These results suggest that the
rate of Cr(VI) reduction depends on the redox reaction of As(III) and As(V) with Cr(VI).
Moreover, the inhibitory effect observed at high Cr(VI) and As(III) concentration may also be attributed to the dual toxicity effect of Cr(VI) and As(III) on microbial cell. From the
above batch kinetic studies lethal concentration of Cr(VI) and As(III) for these strains was
evaluated and established.
Initial evaluation of the bacteria using 16S rRNA partial sequence method showed that cells
in the mixed culture comprised predominantly of the Gram-positive species: Staphylococcus
sp., Enterobacter sp., and Bacillus sp. The biokinetic parameters of these strains were
estimated using a non-competitive inhibition model with a computer programme for
simulation of the Aquatic System “AQUASIM 2.0”.
Microbial reduction of Cr(VI) in the presence of As(III) was further investigated in
continuous-flow bioreactors (biofilm reactor) under varying Cr(VI) loading rates. The reactor
achieved Cr(VI) removal efficiency of more than 96 % in the first three phases of continuous
operation at lower Cr(VI) concentration ranging from 20-50 mg/L. However, 20 % decrease
in Cr(VI) removal efficiency was observed as Cr(VI) concentration increase up to 100 mg/L.
The reactor was able to recover from Cr(VI) and As(III) overloading phase after establishing
the resilient nature of the microorganism. Similarly to the batch reactor studies the overall
performance of the reactor also demonstrated that the presence of As(III) greatly enhance
Cr(VI) reduction in a bioreactor. This was evident by near complete removal of Cr(VI)
concentration up to 50 mg/L. The basic mass balance expressions on Cr(VI) along with the
non-competitive inhibition model were used to estimate the biokinetic parameters in the
continuous flow bioreactor system.
Cr(VI) reduction efficiency along the longitudinal column was also evaluated in this study.
Results showed that Cr(VI) efficiency increased as Cr(VI) concentration travels along the
longitudinal column. Other important factors such as oxygen and pH during biological Cr(VI)
reduction in the presence of As(III) oxidation were also evaluated. / Dissertation (MEng)--University of Pretoria, 2014. / tm2015 / Chemical Engineering / MEng / Unrestricted
Date January 2014
CreatorsIgboamalu, Tony E.
ContributorsChirwa, Evans M.N.
PublisherUniversity of Pretoria
Source SetsSouth African National ETD Portal
Detected LanguageEnglish
Rights© 2015 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria.

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