The aim of this Diploma thesis was to assess the cultivar tolerance of a chosen assortment of butterhead lettuce, leaf lettuce, iceberg lettuce and cabbage to fungal pathogens (Bremia lactucae, Fusarium oxysporum f. sp. conglutinans).
The experiment was conducted in the laboratory conditions of the gardening department FAPPZ of the Czech University of Agriculture in growth chambers. The lettuce experiments were done in given conditions with the temperatures of 18 - 20 °C under the regime 12 hrs. of light and 12 hrs. of darkness. The cabbage experiments were conducted in given conditions under the regime 12 hrs. of light and 12 hrs. of darkness with the temperatures of 14 °C during the day and 12 °C in the night. The lettuce plants were planted in dishes on fine sand. All experiments had infected and control variants. After emergence, the experimental plants were inoculated with variants of the selected pathogen. The plants were observed in 2 - 3 day intervals throughout the whole experiment. The experiment was assessed using the modified standard method according to Pawelec et al. (2006). A modified method with a percentage scale was used to assess the lettuce experiments. Cabbage experiments were evaluated with a scale of points ranging from 0 to 9 according to the percentage of infection on the plants. The results of the methodology were determined using the program Statistica 12.
In the experiments evaluating the variety tolerance of a chosen assortment leaf lettuce and iceberg lettuce to the pathogen Bremia lactucae Bl:31, a statistically significant difference in the sensitivity to this pathogen was established. The results show that the most resistant varieties are Tarzan, Stamir, Adinal a Verala. The greatest sensitivity to the pathogen Bl:31 has a variety "Dětenická Atrakce" then Traper and Nikolaj. Effect of seed treatment with hot water (HWT) to suppress pathogen B. lactucae was statistically significant in the varieties "Dětenická Atrakce" (10% decrease) and Dubáček (7% decrease) compared with untreated infectious variant with HWT. In the sensitivity evaluation of varieties and lines of white cabbage was a statistically significant difference in infecting of pathogen Fusarium oxysporum f. Sp. conglutinans breed 1. The most significantly susceptible to the pathogen Foc was variety Pourovo late-season (degree of assault after inoculation was 6.52 points).
| Identifer | oai:union.ndltd.org:nusl.cz/oai:invenio.nusl.cz:258755 |
| Date | January 2016 |
| Creators | Maláková, Dana |
| Contributors | Koudela, Martin, Kristína, Kristína |
| Publisher | Česká zemědělská univerzita v Praze |
| Source Sets | Czech ETDs |
| Language | Czech |
| Detected Language | English |
| Type | info:eu-repo/semantics/masterThesis |
| Rights | info:eu-repo/semantics/restrictedAccess |
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