A novel plasma cell-restricted E2A-containing ~-tE2-binding species, designated P-E2A, which forms during the mature B- to plasma cell transition has recently been detected (Jacobs, 1993). In order to elucidate the molecular components of P-E2A, a human plasma cell eDNA library was constructed from the ARH-77 cell line, and the yeast two-hybrid system was employed to search for E2A-interacting plasma cell factors. A hybrid vector that expresses the bHLH region of E4 7 was constructed and utilized as bait to detect E2A-interacting clones. Three clones were isolated that represent possible candidates for in vivo E2A interactions. To improve the specificity and sensitivity of the system, we devised an alternative strategy to the yeast two-hybrid system for the in vivo detection ofE-box-binding E2A-interacting proteins.
| Identifer | oai:union.ndltd.org:pacific.edu/oai:scholarlycommons.pacific.edu:uop_etds-3338 |
| Date | 01 January 1998 |
| Creators | Castro, Christopher M. |
| Publisher | Scholarly Commons |
| Source Sets | University of the Pacific |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | University of the Pacific Theses and Dissertations |
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