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<p>Fluorescent proteins and the biosensors created with them
have been used extensively to monitor chemical species inside and outside of
the cell. They have been used to increase our knowledge of cellular function in
normal and diseased states. Fluorescent biosensors are advantageous because
they can be genetically encoded, do not require exogenous reagents, and can be
quantitative. Fluorescent biosensors are also able to measure analytes with
high spatial and temporal resolutions, enabling measurements at the scale of
physiological events. In this thesis efforts have made to increase the
available fluorescent biosensor tools for imaging cellular events. This work includes
creation of new sensors for two molecules not yet detectable via fluorescent
protein biosensor, acetylcholine and adenosine diphosphate. Efforts were also
made to improve the current available biosensors for adenosine triphosphate and
cellular redox, to make them more compatible with multiplex and deep tissue
imaging. Here I present my work to design, characterize and utilize these
fluorescent biosensors.</p>
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| Identifer | oai:union.ndltd.org:purdue.edu/oai:figshare.com:article/8398385 |
| Date | 16 August 2019 |
| Creators | Keelan J Trull (6900062) |
| Source Sets | Purdue University |
| Detected Language | English |
| Type | Text, Thesis |
| Rights | CC BY 4.0 |
| Relation | https://figshare.com/articles/ENGINEERING_FLUORESCENT_PROTEIN_BIOSENSORS_FOR_INTERROGATING_BIOLOGICALLY_RELEVANT_CHEMICAL_SPECIES/8398385 |
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