Insulin-like growth factor (IGF-1) is a potent pro-survival cytokine that is not robustly expressed by human cardiac stem cells (CSCs). Here, we explore the mechanism underlying IGF-1 enhanced cardiac repair by CSCs. Human CSCs underwent lentiviral- mediated somatic gene transfer of IGF-1 to boost cytokine secretion without adversely blunting the overall cytokine signature of CSCs. In vitro studies demonstrated that IGF-1 provided paracrine and autocrine support that reduced apoptosis by CSCs and cardiomyoctes. In vivo experiments demonstrated that IGF-1 increased CSC-mediated cardiac repair by enhancing salvage of reversibly damaged myocardium and transplanted cell survival.
| Identifer | oai:union.ndltd.org:uottawa.ca/oai:ruor.uottawa.ca:10393/31527 |
| Date | January 2014 |
| Creators | Jackson, Robyn |
| Contributors | Davis, Darryl |
| Publisher | Université d'Ottawa / University of Ottawa |
| Source Sets | Université d’Ottawa |
| Language | English |
| Detected Language | English |
| Type | Thesis |
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