Les techniques adaptatives de transmission vidéo s’appuient sur un contenu qui est encodé à différents niveaux de qualité et divisé en segments temporels. Avant de télécharger un segment, le client exécute un algorithme d’adaptation pour décider le meilleur niveau de qualité à considérer. Selon les services, ce niveau de qualité doit correspondre aux ressources réseaux disponibles, mais aussi à d’autres éléments comme le mouvement de tête d’un utilisateur regardant une vidéo immersive (à 360°) afin de maximiser la qualité de la portion de la vidéo qui est regardée. L’efficacité de l’algorithme d’adaptation a un impact direct sur la qualité de l’expérience finale. En cas de mauvaise sélection de segment, un client HTTP/1 doit attendre le téléchargement du prochain segment afin de choisir une qualité appropriée. Dans cette thèse, nous proposons d’utiliser le protocole HTTP/2 pour remédier à ce problème. Tout d’abord, nous nous focalisons sur le service de vidéo en direct. Nous concevons une stratégie de rejet d’images vidéo quand la bande passante est très variable afin d’éviter les arrêts fréquents de la lecture vidéo et l’accumulation des retards. Le client doit demander chaque image vidéo dans un flux HTTP/2 dédié pour contrôler la livraison des images par appel aux fonctionnalités HTTP/2 au niveau des flux concernées. Ensuite, nous optimisons la livraison des vidéos immersives en bénéficiant de l’amélioration de la prédiction des mouvements de têtes de l’utilisateur grâce aux fonctionnalités d’initialisation et de priorité de HTTP/2. Les résultats montrent que HTTP/2 permet d’optimiser l’utilisation des ressources réseaux et de s’adapter aux latences exigées par chaque service. / Adaptive video streaming techniques enable the delivery of content that is encoded at various levels of quality and split into temporal segments. Before downloading a segment, the client runs an adaptation algorithm to determine the level of quality that best matches the network resources. For immersive video streaming this adaptation mechanism should also consider the head movement of a user watching the 360° video to maximize the quality of the viewed portion. However, this adaptation may suffer from errors, which impact the end user’s quality of experience. In this case, an HTTP/1 client must wait for the download of the next segment to choose a suitable quality. In this thesis, we propose to use the HTTP/2 protocol instead to address this problem. First, we focus live streaming video. We design a strategy to discard video frames when the band width is very variable in order so as to avoid the rebuffering events and the accumulation of delays. The customer requests each video frame in an HTTP/2 stream which allows to control the delivery of frames by leveraging the HTTP/2 features at the level of the dedicated stream. Besides, we use the priority and reset stream features of HTTP/2 to optimize the delivery of immersive videos. We propose a strategy to benefit from the improvement of the user’s head movements prediction overtime. The results show that HTTP/2 allows to optimize the use of network resources and to adapt to the latencies required by each service.

Le streaming adaptatif sur HTTP, désigné par HAS, est une technique de streaming vidéo largement déployée sur Internet. Elle utilise TCP comme protocole de transport. Elle consiste à segmenter la vidéo stockée sur un serveur web en petits segments indépendants de même durée de lecture et transcodés à plusieurs niveaux de qualité, désignés par "chunks". Le player, du côté du client HAS, demande périodiquement un nouveau chunk. Il sélectionne le niveau de qualité en se basant sur l’estimation de la bande passante du/des chunk(s) précédent(s). Étant donné que chaque client HAS est situé au sein d’un réseau d’accès, notre étude traite un cas fréquent dans l’usage quotidien: lorsque plusieurs clients partagent le même lien présentant un goulot d’étrangement et se trouvent en compétition sur la bande passante. Dans ce cas, on signale une dégradation de la qualité d’expérience (QoE) des utilisateurs de HAS et de la qualité de service (QoS) du réseau d’accès. Ainsi, l’objectif de cette thèse est d’optimiser le protocole TCP pour résoudre ces dégradations de QoE et QoS. Notre première contribution propose une méthode de bridage du débit HAS au niveau de la passerelle. Cette méthode est désignée par "Receive Window Tuning Method" (RWTM): elle utilise le principe de contrôle de flux de TCP et l’estimation passive du RTT au niveau de la passerelle. Nous avons comparé les performances de RWTM avec une méthode récente implémentée à la passerelle qui utilise une discipline particulière de gestion de la file d’attente, qui est désignée par "Hierarchical Token Bucket shaping Method" (HTBM). Les résultats d’évaluations indiquent que RWTM offre une meilleure QoE et une meilleure QoS de réseau d’accès que HTBM. Notre deuxième contribution consiste à mener une étude comparative combinant deux méthodes de bridages, RWTM et HTBM, avec quatre variantes TCP largement déployées, NewReno, Vegas, Illinois et Cubic. Les résultats d'évaluations montrent une discordance importante entre les performances des différentes combinaisons. De plus, la combinaison qui améliore les performances dans la majorité des scénarios étudiés est celle de RWTM avec Illinois. En outre, une mise à jour efficace de la valeur du paramètre "Slow Start Threshold", sthresh, peut accélérer la vitesse de convergence du player vers la qualité optimale. Notre troisième contribution propose une nouvelle variante de TCP adaptée aux flux HAS, qu’on désigne par TcpHas; c’est un algorithme de contrôle de congestion de TCP adapté aux spécifications de HAS. TcpHas estime le niveau de la qualité optimale du flux HAS en se basant sur l’estimation de la bande passante de bout en bout. Ensuite, TcpHas applique un bridage au trafic HAS en fonction du débit d’encodage du niveau de qualité estimé. TcpHas met à jour ssthresh pour accélérer la vitesse de convergence. Une étude comparative a été réalisée avec la variante Westwood+. Les résultats d’évaluations montrent que TcpHas est plus performant que Westwood+. / HTTP adaptive streaming (HAS) is a streaming video technique widely used over the Internet. It employs Transmission Control Protocol (TCP) as transport protocol and it splits the original video inside the server into segments of same duration, called "chunks", that are transcoded into multiple quality levels. The HAS player, on the client side, requests for one chunk each chunk duration and it commonly selects the quality level based on the estimated bandwidth of the previous chunk(s). Given that the HAS clients are located inside access networks, our investigation involves several HAS clients sharing the same bottleneck link and competing for bandwidth. Here, a degradation of both Quality of Experience (QoE) of HAS users and Quality of Service (QoS) of the access network are often recorded. The objective of this thesis is to optimize the TCP protocol in order to solve both QoE and QoS degradations. Our first contribution consists of proposing a gateway-based shaping method, that we called Receive Window Tuning Method (RWTM); it employs the TCP flow control and passive round trip time estimation on the gateway side. We compared the performances of RWTM with another gateway-based shaping method that is based on queuing discipline, called Hierarchical Token Bucket shaping Method (HTBM). The results of evaluation indicate that RWTM outperforms HTBM not only in terms of QoE of HAS but also in terms of QoS of access network by reducing the queuing delay and significantly reducing packet drop rate at the bottleneck.Our second contribution consists of a comparative evaluation when combining two shaping methods, RWTM and HTBM, and four very common TCP variants, NewReno, Vegas, Illinois and Cubic. The results show that there is a significant discordance in performance between combinations. Furthermore, the best combination that improves performances in the majority of scenarios is when combining Illinois variant with RWTM. In addition, the results reveal the importance of an efficient updating of the slow start threshold value, ssthresh, to accelerate the convergence toward the best feasible quality level. Our third contribution consists of proposing a novel HAS-based TCP variant, that we called TcpHas; it is a TCP congestion control algorithm that takes into consideration the specifications of HAS flow. Besides, it estimates the optimal quality level of its corresponding HAS flow based on end-to-end bandwidth estimation. Then, it permanently performs HAS traffic shaping based on the encoding rate of the estimated level. It also updates ssthresh to accelerate convergence speed. A comparative performance evaluation of TcpHas with a recent and well-known TCP variant that employs adaptive decrease mechanism, called Westwood+, was performed. Results indicated that TcpHas largely outperforms Westwood+; it offers better quality level stability on the optimal quality level, it dramatically reduces the packet drop rate and it generates lower queuing delay.

International Telemetering Conference Proceedings / October 13-15, 1981 / Bahia Hotel, San Diego, California / An on-board baseband processor concept developed for commercial communications satellite systems planned for the 1990s is described. The baseband processor, which operates in a time-division multiple access mode, provides significant advantages in improved link margins and system flexibility in accommodating varying demands from a large number of users because of its ability to route and control message traffic on an individual basis. This concept is currently being implemented in a proof-of-concept model with the main objective of developing the technology required to support detailed design and fabrication for an experimental satellite to be launched in 1987. This technology development includes serial MSK modems, Clos-type baseband routing switch, a singlechip custom CMOS maximum-likelihood convolutional decoder, and custom LSI implementation of high speed, low power ECL building blocks.

International Telemetering Conference Proceedings / October 22-25, 1984 / Riviera Hotel, Las Vegas, Nevada / The paper gives a short history of the beginnings in tele-meteorology and missile telemetry in Germany and describes the developments since the 50’s in these fields, and in remote control systems up to the present day.

Real-time on-site histopathology review of biopsy tissues at the point-of-procedure has great potential for significant clinical value and improved patient care. For instance, on-site review can aid in rapid screening of diagnostic biopsies to reduce false-negative results, or in quantitative assessment of biospecimen quality to increase the efficacy of downstream laboratory and histopathology analysis. However, the only currently available rapid pathology method, frozen section analysis (FSA), is too time-and labor-intensive for use in screening large quantities of biopsy tissues and is too destructive for maximum tissue conservation in multiple small needle core biopsies. In this work we demonstrate the spectrally-compatible combination of the nuclear stain DRAQ5 and the anionic counterstain eosin as a dual-component fluorescent staining analog to hematoxylin and eosin intended for use on fresh, unsectioned tissues. Combined with optical sectioning fluorescence microscopy and pseudo-coloring algorithms, DRAQ5 and eosin ("D&E") enables very fast, non-destructive psuedohistological imaging of tissues at the point-of-acquisition with minimal tissue handling and processing. D&E was validated against H&E on a one-to-one basis on formalin-fixed paraffin-embedded and frozen section tissues of various human organs using standard epi-fluorescence microscopy, demonstrating high fidelity of the staining mechanism as an H&E analog. The method was then applied to fresh, whole 18G renal needle core biopsies and large needle core prostate biospecimen biopsies using fluorescence structured illumination optical sectioning microscopy. We demonstrate the ability to obtain high-resolution histology-like images of unsectioned, fresh tissues similar to subsequent H&E staining of the tissue. The application of D&E does not interfere with subsequent standard-of-care H&E staining and imaging, preserving the integrity of the tissue for thorough downstream analysis. These results indicate that this dual-stain pseudocoloring method could provide a real-time histology-like image at the time of acquisition and valuable objective tissue analysis for the clinician at the time of service.

It is a striking observation that tissue of patients invaded by the deep mycoses often lacks evidence of an inflammatory response. This lack of host response is often attributed to neutropenia secondary to chemotherapy. However, systematic studies do not support this simplistic explanation. However, invasive fungal lesions are characterized by abundant fungal functional amyloid, which in turn is bound by serum amyloid P component (SAP). We postulate that SAP is important in the local immune response in invasive fungal infections. The interaction between fungal functional amyloid, SAP, and the immune response in deep mycoses is discussed.

Background: Adipose-derived stem/stromal cells (ASCs) isolated from the stromal vascular fraction are a source of mesenchymal stem cells that have been shown to be beneficial in many regenerative medicine applications. ASCs are an attractive source of stem cells in particular, due to their lack of immunogenicity. This study examines differences between mitochondrial bioenergetic profiles of ASCs isolated from adipose tissue of five peri-organ regions: pericardial, thymic, knee, shoulder, and abdomen. Results: Flow cytometry showed that the majority of each ASC population isolated from the adipose tissue of 12 donors, with an n = 3 for each tissue type, were positive for MSC markers CD90, CD73, and CD105, and negative for hematopoietic markers CD34, CD11B, CD19, and CD45. Bioenergetic profiles were obtained for ASCs with an n = 4 for each tissue type and graphed together for comparison. Mitochondrial stress tests provided the following measurements: basal respiration rate (measured as oxygen consumption rate [pmol O-2/min], ATP production, proton leak, maximal respiration, respiratory control ratio, coupling efficiency, and non-mitochondrial respiration. Glycolytic stress tests provided the following measurements: basal glycolysis rate (measured as extracellular acidification rate [mpH/min]), glycolytic capacity, glycolytic reserve, and non-glycolytic acidification. Conclusions: The main goal of this manuscript was to provide baseline reference values for future experiments and to compare bioenergetic potentials of ASCs isolated from adipose tissue harvested from different anatomical locations. Through an investigation of mitochondrial respiration and glycolysis, it was demonstrated that bioenergetic profiles do not significantly differ by region due to depot-dependent and donor-dependent variability. Thus, although the physiological function, microenvironment and anatomical harvest site may directly affect the characteristics of ASCs isolated from different organ regions, the ultimate utility of ASCs remains independent of the anatomical harvest site.

Leptospirosis causes significant morbidity and mortality worldwide; however, the role of the host immune response in disease progression and high case fatality (> 10-50%) is poorly understood. We conducted a multi-parameter investigation of patients with acute leptospirosis to identify mechanisms associated with case fatality. Whole blood transcriptional profiling of 16 hospitalized Brazilian patients with acute leptospirosis (13 survivors, 3 deceased) revealed fatal cases had lower expression of the antimicrobial peptide, cathelicidin, and chemokines, but more abundant pro-inflammatory cytokine receptors. In contrast, survivors generated strong adaptive immune signatures, including transcripts relevant to antigen presentation and immunoglobulin production. In an independent cohort (23 survivors, 22 deceased), fatal cases had higher bacterial loads (P = 0.0004) and lower anti-Leptospira antibody titers (P = 0.02) at the time of hospitalization, independent of the duration of illness. Low serum cathelicidin and RANTES levels during acute illness were independent risk factors for higher bacterial loads (P = 0.005) and death (P = 0.04), respectively. To investigate the mechanism of cathelicidin in patients surviving acute disease, we administered LL-37, the active peptide of cathelicidin, in a hamster model of lethal leptospirosis and found it significantly decreased bacterial loads and increased survival. Our findings indicate that the host immune response plays a central role in severe leptospirosis disease progression. While drawn from a limited study size, significant conclusions include that poor clinical outcomes are associated with high systemic bacterial loads, and a decreased antibody response. Furthermore, our data identified a key role for the antimicrobial peptide, cathelicidin, in mounting an effective bactericidal response against the pathogen, which represents a valuable new therapeutic approach for leptospirosis.

Ultrasensitive nanomechanical instruments, including the atomic force microscope (AFM)(1-4) and optical and magnetic tweezers(5-8), have helped shed new light on the complex mechanical environments of biological processes. However, it is difficult to scale down the size of these instruments due to their feedback mechanisms9, which, if overcome, would enable high-density nanomechanical probing inside materials. A variety of molecular force probes including mechanophores(10), quantum dots(11), fluorescent pairs(12,13) and molecular rotors(14-16) have been designed to measure intracellular stresses; however, fluorescence-based techniques can have short operating times due to photo-instability and it is still challenging to quantify the forces with high spatial and mechanical resolution. Here, we develop a compact nanofibre optic force transducer (NOFT) that utilizes strong near-field plasmon-dielectric interactions to measure local forces with a sensitivity of <200 fN. The NOFT system is tested by monitoring bacterial motion and heart-cell beating as well as detecting infrasound power in solution.

No description available.