Stuttgart, Univ., Diss., 2002 (Nicht für den Austausch).

The effect of menthol on nicotine metabolism was examined in liver S9 fractions of four different species and in the in vivo mouse model. The purpose of this study was to investigate three parameters: (1) biotransformation of nicotine to cotinine in various species (human, mouse, rat and trout) using in vitro methods; (2) to determine if the addition of menthol with nicotine altered biotransformation of nicotine to cotinine; (3) and to assess similar parameters in an in vivo mouse model. The major findings of this study include: (1) mice appear to metabolize nicotine, over time, in a manner similar to humans; (2) menthol decreased cotinine production, over time, after a single dose in mice; and (3) menthol increased cotinine production, over time, after repeated doses, in mice.

The primary aim of this study was to determine the effect of high frequency Transcutaneous Electrical Nerve Stimulation (TENS) on cold sensory function following topical application of menthol. Quantitative sensory testing was used to determine cold sensation and cold pain thresholds before and after topical application of a 40% menthol solution in 9 male and 11 female subjects. In a separate session the effect of TENS (100 HZ, constant pulse, 100µs, 20 minutes) was determined on menthol-induced cold sensation. Menthol produced a distinct cold hyperalgesia which was significantly reduced during the application of high frequency TENS. The analgesic effect of TENS persisted beyond the application period for at least 20 minutes. Menthol also reduced cold detection thresholds but TENS had no effect on this aspect of cold sensation. These data support the use of TENS as a means of treating cold hyperalgesia such as that found in neuropathic pain states.

The primary aim of this study was to determine the effect of high frequency Transcutaneous Electrical Nerve Stimulation (TENS) on cold sensory function following topical application of menthol. Quantitative sensory testing was used to determine cold sensation and cold pain thresholds before and after topical application of a 40% menthol solution in 9 male and 11 female subjects. In a separate session the effect of TENS (100 HZ, constant pulse, 100µs, 20 minutes) was determined on menthol-induced cold sensation. Menthol produced a distinct cold hyperalgesia which was significantly reduced during the application of high frequency TENS. The analgesic effect of TENS persisted beyond the application period for at least 20 minutes. Menthol also reduced cold detection thresholds but TENS had no effect on this aspect of cold sensation. These data support the use of TENS as a means of treating cold hyperalgesia such as that found in neuropathic pain states.

by Tsoi Yeung-pang. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1994. / Includes bibliographical references (leaves 105-106). / Chapter I. --- Acknowledgements --- p.i / Chapter II. --- Abstract --- p.ii / Chapter III. --- Table of contents --- p.iv / Chapter IV. --- List of Tables and Figures --- p.v / Chapter Chapter 1. --- Introduction --- p.1 / Chapter 1.1 --- Modes of chromatography / Chapter 1.2 --- Objective of the present study / References / Chapter Chapter 2. --- Instrumentation and theory --- p.8 / Chapter 2.1 --- Instrumentation of HPLC / Chapter 2.2 --- Theory of liquid chromatography / References / Chapter Chapter 3. --- Determination of citrate in pharmaceutical preparations by HPLC using indirect photometric detection --- p.21 / Chapter 3.1 --- Introduction / Chapter 3.2 --- Review of the analytical methods / Chapter 3.3 --- Theory of detection / Chapter 3.4 --- Experimental / Chapter 3.5 --- Results and discussion / Chapter 3.6 --- Conclusion / References / Chapter Chapter 4. --- Determination of camphor and menthol by HPLC using indirect conductometric detection --- p.74 / Chapter 4.1 --- Introduction / Chapter 4.2 --- Review of the analytical methods / Chapter 4.3 --- Theory of detection / Chapter 4.4 --- Experimental / Chapter 4.5 --- Results and discussion / Chapter 4.6 --- Conclusion / References

Erlangen, Nürnberg, University, Diss., 2006. / Enth. 1 Sonderabdr. aus: Neuroreport ; Vol. 16. 2005. - Beitr. teilw. dt., teilw. engl.

University, Diss., 2002--Stuttgart. / Gedr. Ausg. im Inst. für Technische Biochemie der Univ. Stuttgart.

Introduction: An active area of tobacco regulatory science research focuses on examining the effects of varying the nicotine content of cigarettes as part of a potential national policy to lower their nicotine content levels to reduce addiction potential. The present study examines differences in the behavioral effects of reduced nicotine content cigarettes related to their menthol status. Menthol is the only cigarette flavoring that is still legally permissible according to Food and Drug administration regulations. Methods: Participants were 26 current adult smokers from three populations especially vulnerable to tobacco use and addiction (economically disadvantaged women, opioid-dependent individuals, individuals with affective disorders) dichotomized as menthol (n=11) or non-menthol (n=15) smokers. Participants completed 14 experimental sessions following acute smoking abstinence (CO<50% baseline level). Across sessions, participants smoked four Spectrum research cigarettes (22nd Century Group, Clarence, NY) with varying nicotine content levels (0.4mg/g, 2.4 mg/g, 5.2 mg/g, 15.8 mg/g) or their usual brand cigarette. Research cigarettes were mentholated or non-mentholated corresponding to participants usual brand. Upon completion of smoking, participants completed tasks measuring reinforcing efficacy, subjective effects, topography, and withdrawal and craving measures. Repeated Measures Analysis of Variance was used for all analyses (p<.05). Results: Main effects of menthol status, as well as interactions of nicotine dose and menthol were noted across subscales of subjective effects and direct assessments of reinforcing efficacy. Usual brand mentholated cigarettes produced a profile of equal or greater relative reinforcing effects than usual brand non-mentholated cigarettes, while mentholated research cigarettes produced a profile of effects that fell below (i.e., lower relative reinforcing effects compared to usual brand or non-mentholated cigarettes) those of non-mentholated research cigarettes. Conclusions: Mentholated research cigarettes produce a lower profile of reinforcing and subjective effects, without discernible differences in smoking topography. The potential impact of mentholation on reinforcing efficacy and subjective effects should be considered when using Spectrum research cigarettes.

In this report, we analyze trends in interleukin-6 (IL-6) production in people living with human immunodeficiency virus (PLWH), as a function of smoking behaviors and race, using a clinic-based case-control design. Peripheral blood mononuclear cells (PBMCs) were collected from a multiethnic cohort of 120 PLWH to measure IL-6 in culture supernatant. The data indicated that stimulated PBMC produced significantly higher levels of IL-6 in smokers than nonsmokers. However, as expected, this relationship was substantially modified by race. The distinctive production of IL-6 across different racial groups highlights the need for additional studies and suggests that African American smokers have enhanced production of IL-6 than other groups. Additional analyses indicate that higher IL-6 levels are related to the predicted use of mentholated cigarettes, which are more frequently used by African Americans. These findings warrant further investigation and indicate the critical need for tailored preventive interventions.

La consommation de boissons froides et l’utilisation de menthol par voie orale sont des moyens efficaces pour lutter contre des effets néfastes du climat chaud /humide sur la performance aérobie. Les buts de cette thèse étaient 1) de déterminer l’efficacité des effets cumulatifs de l’eau froide et du menthol sur la performance aérobie et 2) d'identifier la capacité de renforcer la performance par la combinaison du précooling interne et du percooling interne en environnement tropical. Les expérimentations étaient des exercices de contre-la-montre, en laboratoire ou en extérieur, dans lesquelles les sujets ont bu des boissons (i. E. , eau neutre, eau froide ou glace pilée) avec ou sans menthol. Les principaux résultats mettent en évidence que l'ingestion d'une boisson au menthol augmente la performanceet cette augmentation s’accentue en diminuant la température du liquide ingéré (étude 1). Cette glace pilée mentholée semble mieux conserver, en conditions écologiques, la capacité d’amélioration de la performance observée en laboratoire (étude 2). Une fois que la glace pilée mentholée est prise au cours de l’effort, l’adjonction pré-exercice d’un refroidissement par boisson froide devient inutile pour accroître davantage la performance (étude 3). Enfin, une boisson froide au menthol permet de limiter le stress psycho-physiologique durant l’exercice (étude 1 et 2). L’ingestion d’eau froide/mentholée ou de glace pilée/mentholée pendant l’exercice semble être une stratégie efficace pour améliorer la performance aérobie et peut être recommandée pour les athlètes lors de compétitions sportives sous climat chaud (sec ou humide). / Cold drink consumption and the use of menthol by mouth are effective ways to fight against harmful effects of hot and humid climate on aerobic performance. The aims of this thesis were 1) to determine the effectiveness of the cumulative effects of the cold water and the menthol on aerobic performance and 2) to identify the ability to enhance performance by combining internal precooling and internal percooling in a tropical environment. The experiments were time trial, in a laboratory or outdoors, in which subjects absorbed beverages (i.e., neutral water, cold water, ice-slurry) with or without menthol. The main results show that the ingestion of beverage/menthol increases exercise performance and this increase is accentuated while decreasing the temperature of ingested beverage (Study 1). The ice-slurry/menthol seems to be better in preserving, in ecological conditions, observed improvement of exercise performance in the laboratory (Study 2). When ice-slurry/menthol is absorbed in the effort, the precooling by adding cold drink becomes unnecessary to further increase the performance (Study 3). Finally, a cold beverage/menthol limits psychological and physiological stress during exercise (Study 1 and 2).The ingestion of cold water/menthol or ice-slurry/menthol during exercise appears to be an effective strategy to improve aerobic performance and is recommended for athletes in sports competitions in a hot climate (dry or wet).