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The Global ETD Search service is a free service for researchers
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Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
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Showing 1 to 2 of 2 (0.076 seconds)Spelling suggestions: "subject:"; chromatin; etabolism"" "subject:"; chromatin; emetabolism""
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p63 regulates Satb1 to control tissue-specific chromatin remodeling during development of the epidermisFessing, Michael Y., Mardaryev, Andrei N., Gdula, Michal R., Sharov, A.A., Sharova, T.Y., Rapisarda, Valentina, Gordon, K.B., Smorodchenko, A.D., Poterlowicz, Krzysztof, Ferone, G., Kohwi, Y., Missero, C., Kohwi-Shigematsu, T., Botchkarev, Vladimir A. January 2011 (has links)
No / During development, multipotent progenitor cells establish tissue-specific programs of gene expression. In this paper, we show that p63 transcription factor, a master regulator of epidermal morphogenesis, executes its function in part by directly regulating expression of the genome organizer Satb1 in progenitor cells. p63 binds to a proximal regulatory region of the Satb1 gene, and p63 ablation results in marked reduction in the Satb1 expression levels in the epidermis. Satb1(-/-) mice show impaired epidermal morphology. In Satb1-null epidermis, chromatin architecture of the epidermal differentiation complex locus containing genes associated with epidermal differentiation is altered primarily at its central domain, where Satb1 binding was confirmed by chromatin immunoprecipitation-on-chip analysis. Furthermore, genes within this domain fail to be properly activated upon terminal differentiation. Satb1 expression in p63(+/-) skin explants treated with p63 small interfering ribonucleic acid partially restored the epidermal phenotype of p63-deficient mice. These data provide a novel mechanism by which Satb1, a direct downstream target of p63, contributes in epidermal morphogenesis via establishing tissue-specific chromatin organization and gene expression in epidermal progenitor cells.
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Pluripotency Factors Determine Gene Expression Repertoire at Zygotic Genome ActivationGao, Meijiang, Veil, Marina, Rosenblatt, Marcus, Riesle, Aileen J., Gebhard, Anna, Hass, Helge, Buryanova, Lenka, Yampolsky, Lev Y., Grüning, Björn, Ulianov, Sergey V., Timmer, Jens, Onichtchouk, Daria 10 February 2022 (has links)
Awakening of zygotic transcription in animal embryos relies on maternal pioneer transcription factors. The interplay of global and specific functions of these proteins remains poorly understood. Here, we analyze chromatin accessibility and time-resolved transcription in single and double mutant zebrafish embryos lacking pluripotency factors Pou5f3 and Sox19b. We show that two factors modify chromatin in a largely independent manner. We distinguish four types of direct enhancers by differential requirements for Pou5f3 or Sox19b. We demonstrate that changes in chromatin accessibility of enhancers underlie the changes in zygotic expression repertoire in the double mutants. Pou5f3 or Sox19b promote chromatin accessibility of enhancers linked to the genes involved in gastrulation and ventral fate specification. The genes regulating mesendodermal and dorsal fates are primed for activation independently of Pou5f3 and Sox19b. Strikingly, simultaneous loss of Pou5f3 and Sox19b leads to premature expression of genes, involved in regulation of organogenesis and differentiation.
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