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The effects of land use and management practices on soil microbial diversity as determined by PCR-DGGE and CLPP.Wallis, Patricia Dawn. January 2011 (has links)
The environmental impact of anthropogenic disturbances such as agriculture, on the
soil ecosystem, and particularly on soil microbial structural and functional diversity,
is of great importance to soil health, conservation and remediation. Therefore, this
study assessed the effects of various land use and management practices on both the
structural (genetic) and functional (catabolic) diversity of the soil bacterial and fungal
communities, at two long-term sites in KwaZulu-Natal. The first site is situated at
Baynesfield Estate, and the second at Mount Edgecombe Sugarcane Research
Institute. At site 1, the land uses investigated included soils under pre-harvest burnt
sugarcane (Saccharum officinarum, Linn.) (SC); maize (Zea mays, Linn.) under
conventional tillage (M); permanent kikuyu (Pennisetum clandestinum, Chiov)
pasture (KIK); pine (Pinus patula, Schiede) plantation (PF); and wattle (Acacia
mearnsii, De Wild) plantation (W), all fertilized; and undisturbed native grassland
(NAT) that had never been cultivated or fertilized. At site 2, a sugarcane (Saccharum
officinarum × S. spontaneum var. N27) pre-harvest burning and crop residue retention
trial was investigated. The treatments studied included conventional pre-harvest
burning of sugarcane with the tops removed (Bto), and green cane harvesting with
retention of crop residues on the soil surface as a trash blanket (T). Each of these
treatments was either fertilized (F) or unfertilized (Fo).
The polymerase chain reaction (PCR), followed by denaturing gradient gel
electrophoresis (DGGE) were used to determine the structural diversity, and
community level physiological profiling (CLPP) using BIOLOG plates, the catabolic
diversity. In addition, the soils were analysed with respect to selected
physicochemical variables, and the effects of these on the soil microbial communities
were determined. Replicate soil samples (0–5 cm) were randomly collected from three
independent locations within each land use and management, at both sites. Soil
suspensions for the CLPP analyses were prepared from fresh soil subsamples (within
24 h of collection) for the bacterial community analyses, and from 8-day-old soil
subsamples (incubated at 4°C to allow for spore germination) for the fungal
community analyses. BIOLOG EcoPlates™ were used for the bacterial CLPP study
and SF-N2 MicroPlates™ for the fungal analysis, the protocols being adapted and optimized for local conditions. This data was log [X+1]-transformed and analysed by
principal component analysis (PCA) and redundancy analysis (RDA). For PCRDGGE,
total genomic DNA was isolated directly from each soil subsample, and
purified using the MO BIO UltraClean™ soil DNA Isolation kit. Protocols were
developed and optimized, and fragments of 16S rDNA from soil bacterial
communities were PCR-amplified, using the universal bacterial primer pair
341fGC/534r. Different size 18S rDNA sequences were amplified from soil fungal
communities, using the universal fungus-specific primer pairs NS1/FR1GC and
FF390/FR1GC. Amplicons from both the bacterial and fungal communities were
fingerprinted by DGGE, and bands in the fungal DGGE gels were excised and
sequenced. The DGGE profiles were analysed by Bio-Rad Quantity One™ Image
analysis software, with respect to band number, position, and relative intensity.
Statistical analyses of this data then followed.
Soil properties [organic C; pH (KCl); exchangeable acidity; total cations (ECEC);
exchangeable K, Ca and Mg; and extractable P] were determined by PCA and were
shown to have affected the structural and catabolic diversity of the resident microbial
communities. At Baynesfield, canonical correspondence analysis (CCA) relating the
selected soil variables to bacterial community structural diversity, indicated that
ECEC, K, P and acidity were correlated with CCA1, accounting for 33.3% of the
variance, whereas Mg and organic C were correlated with CCA2 and accounted for
22.9% of the variance. In the fungal structural diversity study, pH was correlated with
CCA1, accounting for 43.8% of the variance, whereas P, ECEC and organic C were
correlated with CCA2, and accounted for 30.4% of the variance. The RDA of the
catabolic diversity data showed that the same soil variables affecting fungal structural
diversity (organic C, P, ECEC and pH) had influenced both the bacterial and fungal
catabolic diversity. In both the bacterial and fungal RDAs, organic C, P and ECEC
were aligned with RDA1, and pH with RDA2. However in the bacterial analysis,
RDA1 accounted for 46.0%, and RDA2 for 27.5% of the variance, whereas in the
fungal RDA, RDA1 accounted for only 21.7%, and RDA2 for only 15.0% of the
variance.
The higher extractable P and exchangeable K concentrations under SC and M, were
important in differentiating the structural diversity of these soil bacterial and fungal communities from those under the other land uses. High P concentrations under M
were also associated with bacterial catabolic diversity and to a lesser extent with that
of the soil fungal communities under M. Similarly, the higher organic C and
exchangeable Mg concentrations under KIK and NAT, possibly contributed to the
differentiation of these soil bacterial and fungal communities from those under the
other land uses, whereas under PF, the high exchangeable acidity and low pH were
possibly influencing factors. Under W, low concentrations of P and K were noted.
Other factors, such as the presence/absence and frequency of tillage and irrigation,
and the diversity of organic inputs due to the diversity of the above-ground plant
community, (in NAT, for example) were considered potentially important influences
on the nature and diversity of the various land use bacterial and fungal communities.
At Mount Edgecombe, CCA showed that organic C and Mg had a significant effect
on soil bacterial structural diversity. Organic C was closely correlated with CCA1,
accounting for 58.7% of the variance, whereas Mg was associated with CCA2, and
accounted for 41.3% of the variance. In the fungal structural diversity study, ECEC
and pH were strongly correlated with CCA1 and accounted for 49.1% of the variance,
while organic C was associated with CCA2, accounting for 29.6% of the variance. In
the functional diversity studies, RDA showed that both bacterial and fungal
community catabolic diversity was influenced by soil organic C, pH, and ECEC. In
the bacterial analysis, RDA1 was associated with organic C and pH, and accounted
for 43.1% of the variance, whereas ECEC was correlated with RDA2, accounting for
36.9% of the variance. In the fungal analysis, RDA1 was correlated with ECEC and
accounted for 47.1% of the variance, while RDA2 was associated with pH and
organic C, accounting for 35.8% of the variance. The retention of sugarcane harvest
residues on the soil surface in the trashed treatments caused an accumulation of
organic matter in the surface soil, which did not occur in the pre-harvest burnt
sugarcane. This difference in organic C content was a factor in differentiating both
bacterial and fungal communities between the trashed and the burnt treatments. Soil
acidification under long-term N fertilizer applications caused an increase in
exchangeable acidity and a loss of exchangeable Mg and Ca. Thus, as shown by CCA,
a considerably lower exchangeable Mg concentration under F compared to Fo plots
resulted, which was influential in differentiating the bacterial and fungal communities
under these two treatments. In the structural diversity study at Baynesfield, differences were found in bacterial
community species richness and diversity but not in evenness, whereas in the fungal
analysis, differences in community species richness, evenness and diversity were
shown. The soil bacterial and fungal communities associated with each land use were
clearly differentiated. Trends for bacterial and fungal diversity followed the same
order, namely: M < SC < KIK < NAT < PF < W. At Mount Edgecombe, no
significant difference (p > 0.05) in bacterial structural diversity was found with oneway
analysis of variance (ANOVA), but two-way ANOVA showed a slight
significant difference in bacterial community species richness (p = 0.05), as an effect
of fertilizer applications. A significant difference in fungal species richness (p = 0.02)
as a result of management effects was detected, with the highest values recorded for
the burnt/fertilized plots and the lowest for the burnt/unfertilized treatments. No
significant difference was shown in species evenness, or diversity (p > 0.05), in either
the bacterial or the fungal communities.
In the catabolic diversity study at site 1, the non-parametric Kruskal-Wallis ANOVA
showed that land use had not affected bacterial catabolic richness, evenness, or
diversity. In contrast, while fungal catabolic richness had not been affected by land
use, the soil fungal community catabolic evenness and diversity had. At site 2, the
land treatments had a significant effect on soil bacterial community catabolic richness
(p = 0.046), but not on evenness (p = 0.74) or diversity (p = 0.135). In the fungal
study, land management had no significant effect on the catabolic richness (p =
0.706), evenness (p = 0.536) or diversity (p = 0.826).
It was concluded, that the microbial communities under the different land use and
trash management regimes had been successfully differentiated, using the optimized
protocols for the PCR-DGGE of 16S rDNA (bacteria) and 18S rDNA (fungi).
Sequencing bands produced in the 18S rDNA DGGE, enabled some of the soil fungal
communities to be identified. CLPP of the soil microbial communities using BIOLOG
plates showed that, on the basis of C substrate utilization, the soil bacterial and fungal
communities’ catabolic profiles differed markedly. Thus, it was shown that the
different land use and management practices had indeed influenced the structural and
catabolic diversity of both the bacterial and fungal populations in the soil. / Thesis (Ph.D.)-University of KwaZulu-Natal, Pietermaritzburg, 2011.
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Cattle manure, scalping and soil wetness effects on some physical properties of a hardsetting soil and associated early maize growthNciizah, Adornis Dakarai January 2011 (has links)
Most soils in the Eastern Cape Province, South Africa are shallow and are low in organic matter. Therefore these soils are structurally fragile and highly susceptible to inherent degradative processes like hardsetting. The objective of this study was to determine the effect of cattle manure, scalping and soil wetness on aggregate stability, penetration resistance and early maize growth in hardsetting soils. Glasshouse and field studies were conducted to determine the effect of cattle manure on aggregate stability and penetration resistance of freshly exposed topsoils by scalping at 0, 10 and 20 cm depths. In the glasshouse cattle manure was applied at 0 and 20 Mg/ha and matric suction was kept at ~ 30 and ~ 400 kPa; contrasting high and low soil wetness. Three soils were put in pots and arranged in a randomized complete block 3 2 2 factorial design. The field study was done at the University of Fort Hare research farm and the treatments were arranged in a split-plot complete randomized design with three replications. Scalping treatment was the main plot whilst the quantity of the cattle manure applied was the sub plot. Cattle manure increased mean weight diameter (MWD) by between 48% and 71% under glasshouse and between 18% and 33% under field conditions, depending on the soil wetting rate. Cattle manure reduced MWD when the soil under field condition was subjected to mechanical shaking. Soil penetration resistance decreased linearly, with increasing soil wetness but it rapidly increased with increase in matric suction up to ~200 kPa and thereafter the rate of increase reduced. In the glasshouse, all treatments had no significant effects on shoot dry weight but low matric suction increased root dry weight by 133%. Interaction of cattle manure and low matric suction reduced shoot length by 6%, shoot fresh weight by 25%, root surface area by 36%, root length by 5% and root fresh weight by 29% compared to the control. In contrast, application of cattle manure and high matric suction increased shoot length by 37%, shoot fresh weight by 136%, root surface area by 159%, root length by 94% and root fresh weight by 119%. In the field, cattle manure application increased root length density and shoot dry matter by 26% and 30% respectively. Cattle manure improved the stability of aggregates of the hardsetting soil under rapid or slow water intake conditions experienced during rainfall or irrigation. However, under field conditions cattle manure acted as a deflocculant and decreased the stability of aggregates when mechanical stress was applied. The effectiveness of cattle manure in improving maize growth in hardsetting soils was determined by matric suction.
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Holistic characterisation of soils developed on contrasting lithologies, in a temperate climateAshton, Nicola Jane January 2014 (has links)
The influence of parent lithology on the development of soil biogeochemical environments and their microbial diversity is explored by characterising soil profiles with respect to their mineral, solution and organic chemistry. Soil profiles were collected from a total of 17 sites, above basalt, granodiorite, shale, sandstone and limestone, across Northern Ireland. The soil system developed above basalt was examined to assess the development of soil bio-physicochemical properties and microbial diversity through the profile. These basalt soils showed two distinct horizons have developed in the previous 15’000 years, where soils from the top 20 cm of the profile were highly influenced by the interactions of soil minerals with soil organic and biological processes. In line with the observed differences in soil properties the microbial community structure varied; in the surface soils the community composition was dominated by root-associated bacteria. However the relative abundance of phyla affiliated with nutrient-limited conditions increased in samples from the base of the profile. Detailed examination of the soil profiles above granodiorite, shale, sandstone and limestone revealed large variations in soil geochemistry between profiles, reflecting the mineral geochemistry of the parent rock. Molecular analysis of SOM revealed compositional changes with depth were comparable between profiles; however TOC concentrations were consistently higher in the soil profiles above basalt suggesting greater stabilisation of SOM in these soils. The chemistry of the soil waters was not reflective of the parent rocks; however variations in soil texture, specifically the abundance of less reactive residual minerals in the sandstone and limestone soils, led to higher concentrations of soluble elements in these soils. Soil pH and DOC were found to have a large control on buffering the release of free Al, Cr and Fe ions into solution. The microbial communities in near-surface soils were similar to each other, regardless of lithology, and were dominated by Proteobacteria, Actinobacteria, and Acidobacteria. However microbial diversity shifted with depth; the abundance of Actinobacteria decreased and Nitrospirae increased, and between rock types where soils next to the basalt, shale and granodiorite bedrock contained sequences affiliated with novel Candidate Phyla AD3 and GAL15. In these soils differences in SOM composition were the main driver of the observed variation with depth, however where labile SOM was depleted, mineral and solution geochemistry may have a larger control on the community composition. To assess the influence of parent lithology on selenium mobility, soils above basalt and granodiorite were amended with sodium selenate. Under anaerobic conditions, the proportion of soluble selenate removed varied (39-77 %) depending on the sample through a combination of abiotic and microbial reduction processes. However, under aerobic conditions, larger concentrations of selenate remained in solution (79-100%).
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Water movement in a stratified soilSaadi, Abdelhakim. January 1984 (has links)
Call number: LD2668 .T4 1984 S22 / Master of Science
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Soil nailing: a robust design for joint-controlled weathered rock in Hong KongLee, Chun-fai, Julian., 李俊暉. January 2003 (has links)
published_or_final_version / Applied Geosciences / Master / Master of Science
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Soil water supplying capacity as a factor affecting revegetation of cut slopes.January 2007 (has links)
Chiu, Ming Ho. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2007. / Includes bibliographical references (leaves 139-155). / Abstracts in English and Chinese. / Abstract --- p.i / Acknowledgements --- p.v / Table of Contents --- p.vii / List of Tables --- p.xi / List of Figures --- p.xiii / List of Plates --- p.xiv / Chapter Chapter 1 --- Introduction --- p.1 / Chapter 1.1 --- Introduction --- p.1 / Chapter 1.1.1 --- Environment of Hong Kong --- p.1 / Chapter 1.1.1.1 --- Flat land area --- p.1 / Chapter 1.1.1.2 --- Population --- p.2 / Chapter 1.1.1.3 --- Climate --- p.2 / Chapter 1.1.2 --- Landslides in Hong Kong --- p.4 / Chapter 1.1.2.1 --- Landslide history --- p.4 / Chapter 1.1.2.2 --- Government actions on landslide prevention --- p.7 / Chapter 1.1.3 --- Slopes in Hong Kong --- p.8 / Chapter 1.1.4 --- Slope stabilization --- p.10 / Chapter 1.1.4.1 --- Conventional methods of slope stabilization --- p.10 / Chapter 1.1.4.2 --- Biotechnical stabilization --- p.13 / Chapter 1.2 --- Situation in Hong Kong --- p.16 / Chapter 1.2.1 --- Slope protection in the past --- p.16 / Chapter 1.2.2 --- Government action on improving slope appearance --- p.16 / Chapter 1.2.3 --- Proprietary slope greening techniques --- p.19 / Chapter 1.3 --- Vegetation growth on slopes --- p.22 / Chapter 1.3.1 --- Basic requirements of plants --- p.22 / Chapter 1.3.2 --- Potential problems of proprietary systems on shotcreted cut slopes --- p.24 / Chapter 1.3.2.1 --- Steep gradient --- p.24 / Chapter 1.3.2.2 --- Thin soil --- p.24 / Chapter 1.3.2.3 --- Rainfall seasonality --- p.25 / Chapter 1.4 --- Current study --- p.26 / Chapter 1.4.1 --- Objectives --- p.26 / Chapter 1.4.2 --- Significance --- p.26 / Chapter 1.4.3 --- Thesis layout --- p.27 / Chapter Chapter 2 --- Soil water status and vegetation of cut slopes --- p.30 / Chapter 2.1 --- Introduction --- p.30 / Chapter 2.2 --- Materials and methods --- p.36 / Chapter 2.2.1 --- Study site --- p.36 / Chapter 2.2.2 --- In situ measurements and substrate sampling --- p.43 / Chapter 2.2.3 --- Physical properties of substrates on slopes --- p.43 / Chapter 2.2.3.1 --- Slope angle --- p.43 / Chapter 2.2.3.2 --- Substrate thickness --- p.43 / Chapter 2.2.3.3 --- Soil moisture --- p.43 / Chapter 2.2.3.4 --- Soil texture --- p.43 / Chapter 2.2.3.5 --- Bulk density --- p.44 / Chapter 2.2.3.6 --- Water retention capacity --- p.44 / Chapter 2.2.4 --- Chemical properties of substrates on slopes --- p.44 / Chapter 2.2.4.1 --- pH --- p.44 / Chapter 2.2.4.2 --- Conductivity --- p.45 / Chapter 2.2.4.3 --- Organic matter --- p.45 / Chapter 2.2.4.4 --- Total Kjeldahl nitrogen --- p.45 / Chapter 2.2.4.5 --- Mineral nitrogen (ammonium and nitrate) --- p.45 / Chapter 2.2.4.6 --- Carbon:Nitrogen --- p.46 / Chapter 2.2.4.7 --- Total phosphorus --- p.46 / Chapter 2.2.4.8 --- Available phosphorus --- p.46 / Chapter 2.2.4.9 --- Major extractable cations --- p.46 / Chapter 2.2.5 --- Green coverage on slopes --- p.46 / Chapter 2.2.6 --- Statistical analysis --- p.47 / Chapter 2.3 --- Results --- p.47 / Chapter 2.3.1 --- Rainfall characteristics --- p.47 / Chapter 2.3.2 --- Soil moisture --- p.49 / Chapter 2.3.3 --- Green coverage --- p.52 / Chapter 2.3.4 --- Physical properties of substrate on slopes --- p.55 / Chapter 2.3.5 --- Chemical properties of substrate on slopes --- p.57 / Chapter 2.4 --- Discussion --- p.61 / Chapter 2.4.1 --- Soil moisture and vegetation growth --- p.61 / Chapter 2.4.2 --- Soil nutrients and vegetation growth --- p.66 / Chapter 2.4.3 --- Other substrate properties and vegetation growth --- p.69 / Chapter 2.5 --- Summary --- p.75 / Chapter Chapter 3 --- Surface runoff and soil erosion of cut slopes --- p.76 / Chapter 3.1 --- Introduction --- p.76 / Chapter 3.2 --- Materials and methods --- p.84 / Chapter 3.2.1 --- Study site --- p.84 / Chapter 3.2.2 --- Experimental setup --- p.84 / Chapter 3.2.3 --- Surface runoff and soil loss --- p.88 / Chapter 3.2.4 --- Nutrient loss in runoff --- p.89 / Chapter 3.2.4.1 --- Total Kjeldahl Nitrogen --- p.89 / Chapter 3.2.4.2 --- Mineral nitrogen (ammonium and nitrate) --- p.89 / Chapter 3.2.4.3 --- Total phosphorus --- p.89 / Chapter 3.2.4.4 --- Available phosphorus --- p.90 / Chapter 3.2.5 --- Other substrate properties --- p.90 / Chapter 3.2.5.1 --- Soil texture --- p.90 / Chapter 3.2.5.2 --- Bulk density --- p.90 / Chapter 3.2.5.3 --- Soil compaction --- p.90 / Chapter 3.2.5.4 --- Water retention capacity --- p.90 / Chapter 3.2.5.5 --- Organic matter --- p.90 / Chapter 3.2.6 --- Vegetation coverage and green coverage on slope --- p.91 / Chapter 3.2.7 --- Statistical analysis --- p.91 / Chapter 3.3 --- Results --- p.91 / Chapter 3.3.1 --- Meteorological characteristics --- p.91 / Chapter 3.3.2 --- Surface runoff and runoff coefficient --- p.92 / Chapter 3.3.2.1 --- Surface runoff and runoff coefficient between different treatments --- p.92 / Chapter 3.3.2.2 --- Surface runoff and runoff coefficient between different proprietary systems --- p.97 / Chapter 3.3.3 --- Soil loss --- p.98 / Chapter 3.3.3.1 --- Soil loss between different treatments --- p.98 / Chapter 3.3.3.2 --- Soil loss between different proprietary systems --- p.99 / Chapter 3.3.4 --- Nutrient loss --- p.99 / Chapter 3.3.4.1 --- Nutrient loss between different treatments --- p.99 / Chapter 3.3.4.2 --- Nutrient loss between different proprietary systems --- p.104 / Chapter 3.3.5 --- Substrate properties of proprietary systems --- p.104 / Chapter 3.3.6 --- Vegetation coverage and green coverage --- p.107 / Chapter 3.3.7 --- Relationship between rainfall and erosional parameters --- p.110 / Chapter 3.4 --- Discussion --- p.117 / Chapter 3.4.1 --- Surface runoff and runoff coefficient between different treatments --- p.117 / Chapter 3.4.2 --- Relationship between rainfall characteristics and surface runoff --- p.122 / Chapter 3.4.3 --- Soil loss between different treatments --- p.125 / Chapter 3.4.4 --- "Relationship between rainfall characteristics, surface runoff and soil loss" --- p.126 / Chapter 3.4.5 --- Nutrient loss between different treatments --- p.128 / Chapter 3.4.6 --- Surface runoff and erosional losses between different proprietary systems --- p.129 / Chapter 3.5 --- Summary --- p.132 / Chapter Chapter 4 --- Conclusions --- p.134 / Chapter 4.1 --- Summary of major findings --- p.134 / Chapter 4.2 --- Implications of the study --- p.136 / Chapter 4.3 --- Limitations of the study --- p.137 / Chapter 4.4 --- Suggestions for further investigation --- p.138 / References --- p.139 / Appendices --- p.156
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The Influence of Soil Compaction Upon the Thermodynamics of Soil MoistureBox Jr., James E. 01 May 1961 (has links)
The retention of water in soils is a very interesting subject. Soil-water research presents a great challenge to research workers. The challenge is broad in scope and extends from the field problems of large irrigation projects to the atomic scale of the solid-liquid interface.
If scientists are going to describe scientifically soil-water relations, they must ultimately utilize the instruments of science and the language of mathematics. To the end of the latter the mathematics of thermodynamics has been applied in these studies of water retention in soils.
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The effects of salinity and sodicity on soil organic carbon stocks and fluxesWong, Vanessa, u2514228@anu.edu.au January 2007 (has links)
Soil is the worlds largest terrestrial carbon (C) sink, and is estimated to contain approximately 1600 Pg of carbon to a depth of one metre. The distribution of soil organic C (SOC) largely follows gradients similar to biomass accumulation, increasing with increasing precipitation and decreasing temperature. As a result, SOC levels are a function of inputs, dominated by plant litter contributions and rhizodeposition, and losses such as leaching, erosion and heterotrophic respiration. Therefore, changes in biomass inputs, or organic matter accumulation, will most likely also alter these levels in soils. Although the soil microbial biomass (SMB) only comprises 1-5% of soil organic matter (SOM), it is critical in organic matter decomposition and can provide an early indicator of SOM dynamics as a whole due to its faster turnover time, and hence, can be used to determine soil C dynamics under changing environmental conditions.¶
Approximately 932 million ha of land worldwide are degraded due to salinity and sodicity, usually coinciding with land available for agriculture, with salinity affecting 23% of arable land while saline-sodic soils affect a further 10%. Soils affected by salinity, that is, those soils high in soluble salts, are characterised by rising watertables and waterlogging of lower-lying areas in the landscape. Sodic soils are high in exchangeable sodium, and slake and disperse upon wetting to form massive hardsetting structures. Upon drying, sodic soils suffer from poor soil-water relations largely related to decreased permeability, low infiltration capacity and the formation of surface crusts. In these degraded areas, SOC levels are likely to be affected by declining vegetation health and hence, decreasing biomass inputs and concomitant lower levels of organic matter accumulation. Moreover, potential SOC losses can also be affected from dispersed aggregates due to sodicity and solubilisation of SOM due to salinity. However, few studies are available that unambiguously demonstrate the effect of increasing salinity and sodicity on C dynamics. This thesis describes a range of laboratory and field investigations on the effects of salinity and sodicity on SOC dynamics.¶
In this research, the effects of a range of salinity and sodicity levels on C dynamics were determined by subjecting a vegetated soil from Bevendale, New South Wales (NSW) to one of six treatments. A low, mid or high salinity solution (EC 0.5, 10 or 30 dS/m) combined with a low or high sodicity solution (SAR 1 or 30) in a factorial design was leached through a non-degraded soil in a controlled environment. Soil respiration and the SMB were measured over a 12-week experimental period. The greatest increases in SMB occurred in treatments of high-salinity high-sodicity, and high-salinity low-sodicity. This was attributed to solubilisation of SOM which provided additional substrate for decomposition for the microbial population. Thus,
as salinity and sodicity increase in the field, soil C is likely to be rapidly lost as a result of increased mineralisation.¶
Gypsum is the most commonly-used ameliorant in the rehabilitation of sodic and saline-sodic soils affected by adverse soil environmental conditions. When soils were sampled from two sodic profiles in salt-scalded areas at Bevendale and Young, SMB levels and soil respiration rates measured in the laboratory were found to be low in the sodic soil compared to normal non-degraded soils. When the sodic soils were treated with gypsum, there was no change in the SMB and respiration rates. The low levels of SMB and respiration rates were due to low SOC levels as a result of little or no C input into the soils of these highly degraded landscapes, as the high salinity and high sodicity levels have resulted in vegetation death. However, following the addition of organic material to the scalded soils, in the form of coarsely-ground kangaroo grass, SMB levels and respiration rates increased to levels greater than those found in the non-degraded soil. The addition of gypsum (with organic material) gave no additional increases in the SMB.¶
The level of SOC stocks in salt-scalded, vegetated and revegetated profiles was also determined, so that the amount of SOC lost due to salinisation and sodication, and the increase in SOC following revegetation relative to the amount of SOC in a vegetated profile could be ascertained. Results showed up to three times less SOC in salt-scalded profiles compared to vegetated profiles under native pasture, while revegetation of formerly scalded areas with introduced pasture displayed SOC levels comparable to those under native pasture to a depth of 30 cm. However, SOC stocks can be underestimated in saline and sodic landscapes by setting the lower boundary at 30 cm due to the presence of waterlogging, which commonly occurs at a depth greater than 30 cm in saline and sodic landscapes as a result of the presence of high or perched watertables. These results indicate that successful revegetation of scalded areas has the potential to accumulate SOC stocks similar to those found prior to degradation.¶
The experimental results from this project indicate that in salt-affected landscapes, initial increases in salinity and sodicity result in rapid C mineralisation. Biomass inputs also decrease due to declining vegetation health, followed by further losses as a result of leaching and erosion. The remaining native SOM is then mineralised, until very low SOC stocks remain. However, the C sequestration potential in these degraded areas is high, particularly if rehabilitation efforts are successful in reducing salinity and sodicity. Soil ecosystem functions can then be restored if organic material is available as C stock and for decomposition in the form of either added organic material or inputs from vegetation when these salt-affected landscapes are revegetated.
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Dynamic behavior of silty soilsSunitsakul, Jutha 22 September 2004 (has links)
The cyclic resistance of predominantly fine-grained soils has received considerable
attention following ground and foundation failures at sites underlain by silt-rich
soils during recent earthquakes. In several cases substantial ground deformation
and reduced bearing capacity of silt soils has been attributed to excess pore
pressure generation during cyclic loading. These field case studies are significant
due to the occurrence of liquefaction related phenomena in soils that would be
characterized as not susceptible to liquefaction using current geotechnical screening
criteria. The most widely used of these criteria, the "Chinese Criteria" and its
derivatives, are based solely on soil composition and they are essentially diagnostic
tools that categorize the soil in a binary fashion as either liquefiable or non-liquefiable.
The most significant limitations of these screening tools are that they
fail to account for the characteristics of the cyclic loading. This investigation was
undertaken to elucidate the potential for strain development in silts during cyclic
loading, and to develop a practice-oriented procedure for evaluating the seismic
performance of silts as a function of material properties, in situ stresses, and the
characteristics of the cyclic loading.
This dissertation presents the results of a multi-faceted investigation of the potential
for seismically induced pore pressures and large strain development in silt soils.
The primary focus of the research was on the synthesis of laboratory testing results
on fine grained soils. Laboratory data from cyclic tests performed at Oregon State
University and other universities formed the basis for enhanced screening criteria
for potentially liquefiable silts. This data was supplemented with field data from
sites at which excess pore pressure generation, liquefaction, and/or ground failures
were observed during recent earthquakes. This investigation specifically addressed
the behavior of silts during loading in cyclic triaxial tests due to the relative
abundance of data obtained for this test. The data was used in conjunction with
standard geotechnical index tests to enhance an existing energy based procedure for
estimating excess pore pressure generation in silts. This pore pressure model can
be used with the uncoupled, stress-based methods for estimating the post-cyclic
loading volumetric strain developed in this investigation.
The energy-based excess pore pressure model and empirical volumetric strain
relationship were used to calibrate for applications involving silt soils a nonlinear,
effective stress model for dynamic soil response (SUMDES). The SUMDES model
was employed, along with the equivalent linear total stress model SHAKE, to
estimate excess pore pressures generated at un-instrumented field sites that have
exhibited evidence of liquefaction during recent earthquakes. A comparison of the
SUMDES and SHAKE results highlighted the limitations of the latter model for
simulating dynamic soil response at various levels of shaking and pore pressure
response. The results of the SUMDES modeling at several well documented case
study sites are presented in this dissertation. These comparisons are valuable for
demonstrating the uncertainties associated with modeling of the effective stress
behavior of silt during seismic loading. / Graduation date: 2005
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Seasonal variations in the infiltration rate of a Whitehouse soil in southern ArizonaMedina Torres, Jorge Galo, 1951- January 1974 (has links)
No description available.
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