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Overexpression of Manganese Superoxide Dismutase (SOD2) Inhibited the Tumorigenicity of Hepatoma CellsYi, Li-na 11 February 2011 (has links)
Hepatocellular carcinoma (HCC) is one of the most common and devastating malignant tumors in Taiwan. Due to an imbalanced between reactive oxygen species (ROS) production and detoxification, oxidative stress, has been implicated in liver carcinogenesis. Superoxide dismutases (SODS) play a key role in the detoxification of superoxide radicals and thus protect cells from damage induced by free radicals. Manganese superoxide dismutase (MnSOD or SOD2) is a member of the superoxide dismutase family located in mitochondria. SOD2 transforms toxic superoxide, a byproduct of the mitochondrial electron transport chain, into hydrogen peroxide and diatomic oxygen. Though reduced SOD2 protein level and activities have been reported in hepatoma tissues, it remains unclear how SOD2 expression affected the tumorigenic processes of hepatoma cells. Expression analysis of an array of human HCC cell lines revealed that SOD2 were down-regulated in poorly differentiated SK-Hep-1 hepatoma cells. Moreover, SOD2 is downregulated in 68.8% of resected HCC samples (97 out of 141 cases). Adenovirus-mediated SOD2 gene delivery increased the cellular SOD2 protein level and H2O2 production, but reduced the superoxide anion level in SK-Hep-1 cells. Furthermore, SOD2 restoration significantly reduced the proliferation, motility, and colony formation of SK-Hep-1 cells. In vivo animal model, the finding of SOD2 overexpression inhibited the proliferation of Sk-Hep-1 hepatoma cells while reduced the tumor growth in mice. Flow cytometry analysis showed that SOD2 gene transfer inhibited the growth of hepatoma cells through induction of cell cycle arrest at G2/M phase. This was associated with declined cdc2/cdk1 and cyclin B1 expression and upregulation p21Cip1 by SOD2 gene delivery. However, SOD2 overexpression had no effect on the secretion of matrix metalloproteinase-2 (MMP-2) and MMP-9.In conclusion, SOD2 overexpression suppresses the tumorigenicity of hepatoma cells and may hold promise for HCC treatment.
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The Effects Of Hydrogen Peroxide, Gallic Acid And Resveratrol On Growth And Catalase Production Of Aspergillus FumigatusDogan, Tunca 01 February 2008 (has links) (PDF)
The aim of this study was to analyze the effect of hydrogen peroxide and selected phenolic compounds on growth and catalase production of Aspergillus fumigatus. As a result of growing A. fumigatus at different temperatures it was observed that, growth and catalase production of this species were highest at 37 ° / C. Catalase production was highest in the presence of 1 mM H2O2, yielding a significant 3 fold increase with respect to the control. Biomass was also increased by 1,44 fold with respect to the control sample. H2O2 increased catalase production possibly by inducing oxidative stress as biomass production significantly increased after the depletion of H2O2. Both gallic acid and trans-resveratrol significantly enhanced biomass generation of A. fumigatus (1,17 fold increase at 10 mM gallic acid and 1,45 fold increase at 3 mM resveratrol with respect to controls) and decreased extracellular catalase production (4,33 fold at 25 mM gallic acid and 16,7 fold decrease at 3 mM resveratrol with respect to controls) especially in the first 5 or 6 days of the cultivation where the anti-oxidant activity of the compounds were possibly at their maximum. A sudden and significant rise was observed in extracellular catalase activity between 5th and 7th days of the cultivation in phenolic compound applied samples, possibly owing to the depletion of the antioxidant activity of gallic acid and resveratrol followed by fungal cells&rsquo / response to a sudden increase of oxidative stress by boosting catalase production.
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Ozonation Of A Denim Producing Textile Industry WastewaterMorali, Eyup Kaan 01 September 2010 (has links) (PDF)
Denim production is one of the leading sub-sectors of textile industry which basically generates highly colored indigo dyeing effluents. In the present study, ozonation was applied to the indigo-dyeing effluent(COD=820 mg/L / color=5500 Pt-Co), and to the whole effluent from a denim-producing plant before(COD=2750 mg/L / color=3950 Pt-Co) and after(COD=800 mg/L,color=3700 Pt-Co) biological treatment for degradation/detoxification purposes. Ozonation was also tried in the wastewater of the plant(COD=3100 mg/L / color=4500 Pt-Co) that would be produced after some foreseen cleaner production measures / caustic recovery and reusing of dyeing process wastewater.
When applied to indigo-dyeing wastewater / ozonation provided 95% color and 61% COD removals at 1320 mg/h ozone dose within 60 minutes The optimum pH was evaluated as 4 when indigo-dyeing wastewater exposed to ozonation at different pHs(1.6-12.3). On the other side, ozonation applied as pre-treatment to the plant effluent provided 86% color and 46% COD removals with 3240 mg/h ozone dose in 70 minutes. Less satisfactory results were obtained when ozonation was tried in the wastewater after measures, with 86% color and 31% COD removals at 3960 mg/h ozone dose in 80 minutes. When applied to the biologically-treated effluent / at 420
mg/h ozone dose and within 40 minutes, ozonation removed 47% of influent COD and 96% of influent color indicating better performance of ozonation in postoxidation. In order to assess possible improvements on ozonation with the addition of H2O2, different concentrations were tried, but no significant improvement was obtained. The improvement in BOD5/COD index was also determined for the ozonetreated
plant effluent to measure the effects of ozonation on biodegradability and found out that BOD5/COD ratio has improved to 0.39 from 0.22.
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Elektrochemische Untersuchungen von Oxidschichten auf Vanadium und VanadiumlegierungenBachmann, Torsten 06 April 2008 (has links) (PDF)
Elektroden aus Legierungen der Übergangsmetalle Vanadium, Titan und Niob und der reinen Metalle reagieren in Abhängigkeit von der Zusammensetzung und des mit ihnen im Kontakt stehenden wässrigen Elektrolyten in höchst unterschiedlicher Weise. Für eine systematische Untersuchung der elektrochemischen Eigenschaften der Elektroden wurden neben den reinen Metallen binäre und ternäre Legierungen aus Vanadium, Titan und Niob, die jeweils Vanadium enthalten, hergestellt. Es wurden zum ersten Mal zusammenhängend ihre physikalischen und chemischen Eigenschaften durch Strukturuntersuchungen und Untersuchungen der Zusammensetzung der Oberfläche sowie der Morphologie bestimmt. Von den, sich mit einer halbleitenden Oxidschicht überziehenden Metallen, wurden die Halbleitereigenschaften im Elektrolytkontakt studiert, die grundlegenden Korrosionseigenschaften sowie ihr elektrochemisches Verhalten als Elektrodenmaterial in potentiometrischen Zellen und durch Strom-Spannungsmessungen bestimmt. Zur Aufklärung der Kinetik der Oxidschichtbildung wurden potentiostatische Stromtransienten ermittelt und mit bekannten Modellen verglichen.
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On the bleachability of alkaline pulps. The influence of residual lignin structure.Wafa Al-Dajani, Waleed January 2001 (has links)
No description available.
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The cell cycle phase specificity of DNA damage induced by radiation, peroxide and chemotherapeutic drugs targeting topoisomerase II, and CD4 and CD8 receptor expression on apoptotic human lymphocytes /Potter, Alan J. January 2003 (has links)
Thesis (Ph. D.)--University of Washington, 2003. / Vita. Includes bibliographical references (leaves 128-159).
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An optical fiber sensor for the determination of hydrogen peroxideHu, Xue-Mei, January 2008 (has links)
Thesis (M.S.)--Mississippi State University. Department of Chemistry. / Title from title screen. Includes bibliographical references.
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Reactivity and galvanic interactions between sulphide minerals in acidified hydrogen peroxide.Lephuting, Senzeni Sipho. January 2013 (has links)
M. Tech. Metallurgical Engineering. / Aims to provide a mineralogical basis for understanding the galvanic dissolution and interaction of sulphide ores in presence of hydrogen peroxide using electrochemical technique. The research aim to achieve the following objectives: study the mineralogical characteristics of different sulphide ores ; study the sulphuric acid dissolution behaviour of the sulphide ores in the presence of hydrogen peroxide and to investigate the interaction of the mineralogy on the ores and hydrogen peroxide during dissolution.
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Effect of nitrogen doping on the electronic and catalytic properties of carbon nanotube electrode materialsWiggins-Camacho, Jaclyn Dawn 22 June 2011 (has links)
This dissertation discusses the influence of nitrogen doping (N-doping) on the electronic and catalytic properties of carbon nanotubes (CNTs). These properties have been studied using a variety of techniques, in order to both qualitatively and quantitatively analyze the relationship between the nitrogen concentration and observed properties. Chapter 1 provides a general overview of CNTs and N-doping and details some of the previous research from our group. Chapter 2 discusses the assembly and characterization of free-standing electrode mats, which are used in order to understand the intrinsic physicochemical properties of the material without relying on the secondary influence of another conductive support. Raman microscopy, X-Ray photoelectron spectroscopy, scanning and scanning-tunneling electron microscopy, as well as electrochemical methods were all used to demonstrate the viability of the mat electrodes for further experiments. Chapter 3 addresses the examination of a range of nitrogen concentrations in order to better understand the effects of nitrogen concentration on the electrochemical and electrical properties such as the differential capacitance, density of states at the Fermi level (D(E[subscript F])), bulk conductivity and work function. These properties were studied using a variety of techniques, including UV-photoelectron spectroscopy, electrochemical impedance spectroscopy and conductive four point probe. Chapter 4 investigates the inherent catalysis of the nitrogen doped CNTs (N-CNTs) with respect to O2 reduction, and a complex mechanism is proposed. Electrochemical methods such as cyclic and linear sweep voltammetries as well as thermo-gravimetric analysis and gasometric analysis were all employed to determine heterogeneous decomposition rates as well as to detect intermediates of the O₂ reduction reaction. Chapter 5 discusses the electrocatalytic degradation of free cyanide (CN⁻) at the N-CNT mat electrodes. These results both provide further support for the mechanism discussed in Chapter 4, and present the opportunity for a potential application of N-CNTs for environmental purposes. Specifically, spectroscopic and electrochemical methods, in conjunction with theoretical models show both that the presence of CN⁻ does not inhibit O2 reduction, and that it can be effectively converted to cyanate (OCN⁻) at the N-CNT electrodes. Future work involving the assembly and characterization of transparent N-CNT films is discussed in Chapter 6. / text
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Radiation induced corrosion of copperBjörkbacka, Åsa January 2015 (has links)
The process of radiation induced corrosion of copper is not well understood. The most obvious situation where the knowledge of this process is crucial is in a deep repository for high level spent nuclear fuel where the fuel will be sealed inside copper canisters. The radiation will penetrate the canisters and be absorbed by the surrounding environment. In this study gamma irradiations of polished and pre-oxidized copper cubes in anoxic pure water, air of 60-100 % RH and in humid argon were performed. The copper surfaces were examined using IRAS, XPS, cathodic reduction, SEM, AFM, and Raman spectroscopy. The concentration of copper in the reaction solutions was measured using ICP-OES. Also the formation of oxidative species caused by radiation absorption of water was studied by numerical simulations using MAKSIMA software. The corrosion of copper during gamma irradiation vastly exceeds what is expected. The production of oxidative species caused by radiation absorption of water is hundreds of times too low to explain the amount of oxidized copper. A possible explanation for this mismatch is an enhanced radiation chemical yield of HO· on the copper surface. Another one is an increased surface area due to oxidation of copper. One speculation is that HO· interacting with the copper oxide can cause oxidation of the metal. If the thermodynamic driving force is large enough then electrons can be conducted from the metal through the oxide to the oxidant. A dramatic increase in surface area together with an increased interfacial yield of HO· might explain the radiation enhanced corrosion process. / <p>QC 20151022</p>
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