In this study, the effect of implementing Network Coding on the aggregated throughput in Wireless Mesh Networks, was examined. Wireless Mesh Networks (WMNs) are multiple hop wireless networks, where routing through any node is possible. The implication of this characteristic, is that messages flow across the points where it would have been terminated in conventional wireless networks. User nodes in conventional wireless networks only transmit and receive messages from an Access Point (AP), and discard any messages not intended for them. The result is an increase in the volume of network traffic through the links of WMNs. Additionally, the dense collection of multiple RF signals propagating through a shared wireless medium, contributes to the situation where the links become saturated at levels below their capacity. The need exists to examine methods that will improve the utilisation of the shared wireless medium in WMNs. Network Coding is a coding and decoding technique at the network level of the OSI stack, aimed to improve the boundaries of saturated links. The technique implies that the bandwidth is simultaneously shared amongst separate message flows, by combining these flows at common intermediate nodes. The number of transmissions needed to convey information through the network, is decreased by Network Coding. The result is in an improvement of the aggregated throughput. The research approach followed in this dissertation, includes the development of a model that investigates the aggregated throughput performance of WMNs. The scenario of the model, followed a typical example of indoors WMN implementations. Therefore, the physical environment representation of the network elements, included an indoors log–distance path loss channel model, to account for the different effects such as: power absorption through walls; and shadowing. Network functionality in the model was represented through a network flow programming problem. The problem was concerned with determining the optimal amount of flow represented through the links of the WMN, subject to constraints pertaining to the link capacities and mass balance at each node. The functional requirements of the model stated that multiple concurrent sessions were to be represented. This condition implied that the network flow problem had to be a multi–commodity network flow problem. Additionally, the model requirements stated that each session of flow should remain on a single path. This condition implied that the network flow problem had to be an integer programming problem. Therefore, the network flow programming problem of the model was considered mathematically equivalent to a multi–commodity integer programming problem. The complexity of multi–commodity integer programming problems is NP–hard. A heuristic solving method, Simulated Annealing, was implemented to solve the goal function represented by the network flow programming problem of the model. The findings from this research provide evidence that the implementation of Network Coding in WMNs, nearly doubles the level of the calculated aggregated throughput values. The magnitude of this throughput increase, can be further improved by additional manipulation of the network traffic dispersion. This is achieved by utilising link–state methods, rather than distance vector methods, to establish paths for the sessions of flow, present in the WMNs. / Thesis (M.Ing. (Computer and Electronical Engineering))--North-West University, Potchefstroom Campus, 2012.

The application of ultrahigh-field Fourier transform ion cyclotron resonance mass spectrometry (FTICR-MS) technology to identify and quantify metabolomics data is relatively new. An important feature of the FTICR-MS metabolomics data is the high percentage of missing values. In this thesis, missing value analysis showed that the missing value percentages were up to 50% and the control treatment, NaOH.ww, had the highest missing value percentage among the treatments in the aqueous FTICRMS sets. A simulation study was done for the FTICR-MS data to compare selection methods, the Kruskal-Wallis test and the MTP and Limma functions in Bioconductor, an open source project to facilitate the analysis of high-throughput data. The study showed that MTP was sensitive to variations among treatments, while the Kruskal- Wallis test was relatively conservative in detecting variations. As a result, MTP had a much higher false positive rate than Kruskal-Wallis test. The performance of Limma for sensitivity and false positive rate was between the Kruskal-Wallis test and MTP. Data sets with missing values were also simulated to assess the performance of imputation methods. Study showed that variances among treatments diminished or disappeared after imputations, but no new differentially expressed masses were created. This gave us confidence in using imputation methods. Summary of analysis results of some of the frogSCOPE data sets was given in the last chapter as an illustration. / Graduate

Fatty acid profiling provides information on dietary intakes and an understanding of lipid metabolism. High throughput techniques such as fingertip prick (FTP) sampling has gained popularity in recent years as a simplified method for basic research, and could be further used to assess disease risk in the population, and other similar high-throughput techniques have the potential to assist in the monitoring and labeling of fatty acids in the food supply. With the advancement of high-throughput sample analysis techniques, a more complete understanding of storage stability is required as a larger volume of samples are produced with equal amounts of time to analyze them. Energy-assisted analysis techniques have the potential to help ameliorate some of these issues. Presently, FTP blood, whole blood and salmon storage stability is assessed under various storage conditions, and both microwave-assisted direct transesterification and indirect ultrasound-assisted extraction techniques are assessed. It is determined that storage of FTP blood and whole blood samples at -20°C results in significant and nearly complete highly unsaturated fatty acid (HUFA) degradation compared to all other temperatures examined. This degradation is determined to be the result of hemolysis and subsequent iron release from erythrocytes initiating fatty acid peroxidation reactions. Direct transesterification of FTP blood is reduced from as long as three hours to one minute with microwave-assisted energy and fatty acid extraction from ground flaxseed is reduced to 40 minutes from as long as 24 hours without compromising fatty acid profiles. Results of the current study provides insight into the storage stability of food sample and blood samples collected via high-throughput techniques, and provides support for the utilization of further high-throughput energy-assisted analytical methods that can help to minimize the potentially detrimental effects that long-term storage can have on fatty acid profiles.

Until recently, multiple SPME fibres could not be automatically evaluated in a single sequence without manual intervention. This drawback had been a critical issue until recently, particularly during the analysis of numerous on-site samples. Recently, GERSTEL® has developed and commercialized a Multi-Fibre Exchanger (MFX) system designed to overcome this drawback. In this research, a critical evaluation of the MFX performance in terms of storage stability and long term operation is presented. It was established in the course of our research that the MFX can operate continuously and precisely for over 200 extraction/injection cycles. However, when the effect of residence time of commercial fibres on the MFX tray was evaluated, the results have shown that amongst the evaluated fibre coatings, carboxen/polydimethylsiloxane (CAR/PDMS) was the only coating capable of efficient storage on the MFX tray for up to 24 hours after field sampling without suffering significant loss of analytes. Additionally, the MFX system capability for high-throughput analysis was demonstrated by the unattended desorption of multiple fibres after on-site sampling of two different systems, indoor air and biogenic emissions. Subsequently, a protocol based on a new, fast, reproducible, reusable and completely automated method that enables quick assessment of SPME coatings was developed. The protocol consists of an innovative in-vial standard generator containing vacuum pump oil doped with McReynolds probes and subsequently mixed with a polystyrene-divinylbenzene resin. According to our results, the protocol has proven to be a useful tool for the quick assessment of inter-fibre reproducibility prior to their application in on-site analysis. The implications of such protocols include, but are not limited to: time-saving, assurance of reliable and reproducible data, and a dependable guide for novice users of the technique. Finally, an innovative, reusable and readily deployable pen-like diffusive sampler for needle traps (PDS-NT) is proposed. Results have shown that the new PDS-NT is effective for air analysis of benzene, toluene, and o-xylene (BTX). In addition, no statistically significant effects of pen geometry on the uptake of analytes were found.

The colony formation assay (CFA) is the gold standard for measuring cytotoxic effects on cells. To increase efficiency, the CFA was converted to a 96-well format using an automated colony counting algorithm. The 96-well CFA was validated using ionizing radiation (IR) on the FaDu and A549 cancer cell lines. Its ability to evaluate combination therapies was investigated using cisplatin and IR. The 96-well CFA was transferred to a robotic platform for evaluation as a high-throughput screen (HTS) readout for the discovery of novel anti-cancer compounds, and radiosensitizers. Screening yielded eight putative anti-cancer hits, and five putative radiosensitizing hits. Secondary screening confirmed 6/8 anti-cancer compounds, and 0/5 radiosensitizing compounds. Thus, the 96-well CFA can be adopted as an alternative assay to the 6-well CFA in the evaluation of cytotoxicity in vitro, providing a possible readout to be utilized in HTS for discovering anti-cancer compounds, but with limited applicability in discovering radiosensitizers.

Lignin is a major chemical component of plants and the second most abundant natural polymer after cellulose. The concerns and interests of agriculture and industry have stimulated the study of genes governing lignin content in plants in an effort to adapt plants to human purposes. Arabidopsis thaliana provides a convenient model for the study of the genes governing lignin content because of its short growth cycle, small plant size, and small completely sequenced genome. In order to identify the genes controlling lignin content in Arabidopsis accessions using Quantitative Trait Locus (QTL) analysis, a rapid micro-scale method of lignin determination is required. The acetyl bromide method has been modified to enable the rapid micro-scale determination of lignin content in Arabidopsis. Modifications included the use of a micro-ball mill, adoption of a modified rapid method of extraction, use of an ice-bath to stabilize solutions and reduction in solution volumes. The modified method was shown to be accurate and precise with values in agreement with those determined by the conventional method. The extinction coefficient for Arabidopsis lignin, dissolved using acetyl bromide, was determined to be 23.35 g-iLcm-1. This value is independent of the Arabidopsis accession, environmental growth conditions and is insensitive to syringyl/guaiacyl ratio. The modified acetyl bromide method was shown to be well correlated with the 72% sulfuric acid method once the latter had been corrected for protein contamination and acid-soluble lignin content (R² = 0.988, P < 0.0001). As determined by the newly developed acetyl bromide method and confirmed by the sulfuric acid method, lignin content in Arabidopsis was found to be a divergent property. Lignin content in Arabidopsis was found to be weekly correlated with growth rate among Arabidopsis accessions (R² = 0.48, P = 0.011). Lignin content was also found to be correlated with plant height among Arabidopsis accessions (R² = 0.491, P < 0.0001).

Zugl.: Hamburg, Techn. Univ., Diss., 2008

Thesis (Ph. D.)--Ohio State University, 2008. / Title from first page of PDF file. Includes bibliographical references (p. 210-232).

Thesis (Ph. D.)--University of Texas at Austin, 2008. / Vita. Includes bibliographical references.

Darmstadt, Techn. Univ., Diss., 2005. / Dateien im PDF-Format