"Project Reformation" presents the process of change Amanda and Robert Bowen went through as they came to recognize Holy Spirit, the part of Trinity that was sent after the death of Jesus (John 15:26). The documentary combines various filming techniques such as observational footage, reenactments, interviews, and CGI to convey the story. This film captures the walk Robert and Amanda took as they re-examine their past, progressing through a series of supernatural encounters into a recognition of purpose and plan behind the events and experiences. While looking back on this journey, the director seeks to reveal the truth that Holy Spirit is in fact "alive and well," and walks with individuals in their daily lives by sharing how the "gifts" of miracles, healing, visions and prophecy are active even in today's age. In addition, the director desires to reveal the specific message this couple has received through this process: Not to only recognize Holy Spirit, but to reveal the "why" He is now making Himself known in these ways when many would say He has been silent for at least a generation: For reconciliation and restoration, leading to Reformation through Spiritual Healing in Family Ties (Reformation.SHIFT).

This thesis is a study of the soteriological thought of five leading English Reformers from the period 1525-56. In Part One, the soteriological aspects of the thought of William Tyndale, John Frith, and Robert Barnes are examined. These three men all came to Reformation convictions from backgrounds in Catholic Humanism. They were also deeply influenced by Martin Luther, although their writings demonstrate independence of thought, and reveal areas where they differ from him. While the three Reformers each have different emphases, two major facts emerge concerning their soteriological positions: all three exhibit a greater concern for good works than is generally evident in Luther, although their difference with him is fundamentally one of emphasis, not of substance; and all three agree that justification is by faith alone. In Part Two, examination is made of the thought of John Hooper and of John Bradford, especially in relation to two controversies over the nature of election. Hooper's position is framed in opposition to Calvinist predestinarianism, and is dependent upon both Bullinger and Melanchthon. Indeed, he even adopts the synergism of the latter in his reaction against any notion of predestination which divorces election from the actual faith of the individual. In contrast, John Bradford, in opposition to a sect holding Pelagian views, proposes a doctrine of election which reflects much of the predestinarianism of his friend Bucer. While there are tensions in his theology, which indicate that he is perhaps not entirely happy with the implications of his position, his unequivocal adherence to a doctrine of the decree, his emphasis on union with Christ, and his expression of limited atonement, demonstrate that his own position is fundamentally antithetical to that of Hooper.

This thesis considers the contribution of seventeenth-century responses to the Polity towards the creation of Hooker's Anglican identity. It begins with an examination of the growing tensions between the old Refonned understanding of Hooker, and the new Laudian desire to comprehend the Polity as the expression of a distinctive doctrinal religious settlement. Although the dominance of the latter group was temporarily eclipsed by the Civil War it was their understanding of Hooker which emerged as the authentic opinion of the English Church at the Restoration. The examination of the Restoration response to Hooker considers how his recently established image as an Anglican father was perpetuated, the methods used to suppress rival assessments, and the weaknesses of this interpretation. The accession of the Catholic James effectively challenged the Restoration Hooker-sponsored belief in passive obedience, and challenged his Anglican credentials through the large numbers of Catholics who cited the Polity in support of the Roman Church. The long term effects of this upon Hooker are evaluated during the reign of William and Mary. The Whig desire to justify William encouraged them to exploit Hooker's belief in an original political compact, and to encourage more latitudinarian ideas within the Church. Restoration ideologies, however, were far from moribund. Several Tories were able to reconcile their opinions to the change of monarchs, and others waited until the reign of Anne where they endeavoured to put the political and religious clock back. This dominance was only temporary, however, since the advent of the Hanoverians led to the swift resurgence of the Whigs. Nevertheless this did nothing to undermine the now universal belief that Hooker was the leading exponent of the English Church. Although Hooker had anticipated that the Polity would be read as, a Reformed text, it had been turned into a specifically Anglican work within a century of his death.

Lysosomes are intracellular organelles that were considered for a long time to be simply an acidic and hydrolytically active end point of trafficking routes for degradation, in the last 20 years, light has been shed on their functional heterogeneity and striking role in signalling and nutrient homeostasis. While the dynamic nature and variety of lysosomal functions are now better appreciated, the mechanisms governing lysosomal fusion, reformation, signalling, and homeostasis remain to be fully elucidated, and are investigated here. In this study, endolysosomes which formed by fusion of late endosomes with lysosomes and are thought to be the predominant site of hydrolytic activity, were further characterised. Using live cell imaging and fluorescent labelling, the proportion of endolysosomes in the total pool of lysosomes was estimated using probes to their acidity and cathepsin activity, and their larger size compared to storage lysosomes was observed. The endolysosomal membrane was also shown to be marked by Rab7, Rab9, PI(3,5)P2 supporting the role of endolysosomes a highly active and dynamic principal site of hydrolase activity. The contributions of VAMP7 and VAMP8 to endolysosome fusion, measured by delivery of endocytosed cargo from late endosomes to endolysosomes, were analysed by CRISPR-Cas9 mediated knockout. Cells lacking VAMP7 and VAMP8 had no effect on delivery to endolysosomes, however at EM level, they displayed extensive tethering between late endocytic organelles, and accumulated small tethered vesicles. YKT6 knockdown impeded delivery to endolysosomes in VAMP7+VAMP8 knockout cells, which was rescued by VAMP7 expression, suggesting YKT6 substituted for VAMP7 in lysosome fusion. Following the hypothesis that reversible dissociation of V1 and Vo sectors of the V- ATPase may control the increase in pH of reforming storage lysosomes, cells expressing tagged V1G1 and Voa3 were generated. These markers of both sectors are present on endolysosomal membranes, and on the emerging endolysosomal tubules, suggesting the V1 and Vo sectors remain associated at this earliest stage of lysosome reformation, but these markers are still in development. IV Two assays were developed to give a readout of, and assess lysosomal stress. Firstly, an assay measuring TFEB-GFP translocation to the nucleus gave a robust and quantifiable readout of lysosomal perturbation. Secondly, a qPCR assay was developed to measure lysosomal gene upregulation as a downstream reporter of TFEB-activating lysosomal perturbations, however this assay, despite being more lysosome-specific, lacked the consistency and dynamic range of the TFEB translocation quantification. In summary, lysosomes are a heterogeneous collection of organelles, which have been better characterised primarily according to their acidity and hydrolytic capacity. Additionally, more SNAREs appear to be involved in lysosome fusion in cells than suggested by cell free assays, and I have developed tools to trace the V-ATPase during reformation of lysosomes after fusion to form endolysosomes. Lastly, I have developed a robust, reporter for a range of lysosomal stress-inducing conditions, providing a broad indication of their effects on lysosomal signalling and homeostasis.

Thesis (Ph. D.)--Ohio State University, 1974. / Includes bibliographical references (leaves 297-306). Available online via OhioLINK's ETD Center.

Diss.--Fribourg. / Includes bibliographical references.

Thèse--Faculté de théologie protestante de Montauban.

Inaug.-Diss.--Univerität Erlangen-Nürnberg, Erlangen. / Bibliography: p. xii-xlvi.

Thesis (Ph. D.)--University of Queensland, 2002. / Includes bibliographical references.

In recent years, several scholars of the Reformation have worked to complicate the notion of early Protestantism as a singular and liberating hermeneutic movement. In particular, critics like James Simpson and Ramie Targoff have targeted Tudor Bible scholarship as stifling and restrictive. Looking at Elizabethan psalm explications, I see neither a purely liberating nor a purely restrictive hermeneutic culture, but instead a combination of the literal and the figurative, of poetics and theology. Examining three different psalm explications by Martin Luther, John Hooper, and Thomas Wilcox, I find a wide variety of approaches to the Psalms, which suggests a relative interpretive freedom even among the Elizabethan Protestant elite. This analysis leads me to conclude that even early in the development of Protestant England there was no such thing as a unified Protestantism, but instead a patchwork of methods that trace back to humanism and Catholicism as well as emerging theories of literalism and poetics. / text