Brief periods of skeletal muscle ischemia (ischemic pre-conditioning) alter cellular metabolism in a way that confers protection over subsequent ischemic episodes. The mechanisms behind this effect have been studied indirectly through assays for the byproducts of ATP synthesis and in vitro studies of cellular signaling cascades and ROS generation. There have been no direct, in vivo assessments of the changes in respiration during reperfusion. We employed phosphorescence quenching microscopy in conjunction with a flow-arrest technique to assess the influences of external, pressure-induced 1- to 10-min focal ischemia on interstitial oxygenation (PISFO2) and the consumption of oxygen (VO2) in spinotrapezius muscles of Sprague-Dawley rats. During reperfusion following an ischemic period VO2 was measured by the rate of PISFO2 decline during brief, serial flow-arrest compressions. Our tests of this intermittent compression technique indicate that 5 s of flow-arrest followed by 15 s of flow restoration allow measurement of VO2 without compromising baseline or reperfusion recovery of PISFO2. There was a steady rise in VO2 during early reperfusion which was correlated with increasing ischemic durations. Treatment with cyanide confirmed that at least some of this increase was due to an upregulation of cytochrome c oxidase activity. Nitric oxide (NO) suppressed VO2 during rest and reperfusion, while L-NAME did not influence respiration under normoxic conditions. L-NAME produced a significant rise in VO2 under hypoxic conditions following 10 minutes of ischemia, indicating a greater role of NO in the regulation of respiration during low PISFO2 conditions. We conclude that physiological levels of NO regulate mitochondrial respiration during hypoxia and confirm that pharmacological elevation of [NO] reduces VO2 in a manner consistent with the ischemic pre-conditioning effect.

Title: Analysis of self-medication with antibiotics in Kosovo Student: Ardita Veseli Supervisor: Prof. PharmDr. Jiří Vlček, Ph.D. Specialist Supervisor: Mgr. T. Belkina Department of Social and Clinical Pharmacy, Charles University in Prague, Faculty of Pharmacy in Hradec Králové Background: Self-medication is described as a behavior during which someone utilizes drugs for the purpose of treating self-diagnosed symptoms or disorders or the intermittent or continued use of a prescribed drug for chronic or recurrent disease or symptoms. When certain individuals self-medicate with antibiotics, they do it without any prior consultation with a physician. The antibiotics are bought from community pharmacies without a prescription, left-over antibiotics are used or they simply decide to follow the advice given from family members, neighbors or friends. Even though antibiotics are considered revolutionary therapeutic agents in order to eradicate microbes, the repeated and improper antibiotic consumption is contributing to the increased prevalence of antibiotic resistance which nowadays is one of the world's most pressing health problems. Aim: The main goal of the theoretical part is to describe self-medication with antibiotics, the patterns influencing it and some complications associated with this...

Title of master thesis Physiological response and physical activity during climbing wall in school age children Work objectives Determinaton of specific oxygen uptake during climbing in the climbing wall in children. Methods The study involved 10 boys and 9 girls. Their climbing ability was in range 4 to 5+ degree of UIAA (Union Internationale des Associations d'Alpinisme). Maximal oxygen consumption was measured on climbing wall. The climbers climbed two routes, the first vertical profile (90ř) and second overhanging profile (110˚). The participants climbed the wall with a self-selected speed. They climbed the route twice to steady state of physiological response during climbing and to simulate real average wall (15 m). To make conditions equal, everybody had 30 s to get down and start climbing again. Results The girls achieved average of specific oxygen consumption VO2peak 37,1 ± 4,8 ml·kg- 1 ·min-1 in the vertical profile and in the overhanging profile was 40,6 ± 11,5 ml·kg- 1 ·min-1 . The boys achieved slightly higher average of specific oxygen consumption in both profile 39,1 ± 4,8 ml·kg-1 ·min-1 in the vertical profile and 42,1 ± 2,6 ml·kg-1 ·min-1 in the overhanging profile. The girls had average peak heart rate 179 ± 10 beats·min-1 in the vertical profile and boys 170 ± 14 beats·min-1 ....

Charles University in Prague Faculty of Pharmacy in Hradec Kralove Department of Social and Clinical Pharmacy The analysis of pharmacotherapy in patients suffering from hyperlipidemia (Diploma Thesis) Mentor of Diploma Thesis Prof. PharmDr. Jiří Vlček, Ph.D. Mavrovouniotis Konstantinos- Parmenion Hradec Králové 2015 ABSTRACT ENGLISH The analysis of pharmacotherapy in patients suffering from hyperlipidemia Mavrovouniotis Konstantinos Mentor: Prof. Dr. Jiří Vlček, CSc. Introduction: Hyperlipidemia is a serious condition whereby the blood levels of lipids, cholesterol and triglycerides are abnormally elevated. This condition is generally asymptomatic but may lead to atherosclerotic heart disease and other types of cardiovascular pathology if not treated effectively. Various risk factors can affect the onset and severity of hyperlipidemia and those include genetic predisposition, hypertension, diabetes mellitus, obesity, poor diet, lack of regular exercise, smoking etc. Measurement and monitoring of blood levels of triglycerides, cholesterol and lipoproteins can be used as prevention method for assessing the risk for an individual to develop hyperlipidemia. Once a patient is diagnosed with the condition several approaches exist for the treatment. The main goal of the treatment is to reduce the risk of...

In just forty years, the number of mobile devices has exceeded the number of humans on Earth. With the growing computational capacity, the power demands of these devices are growing rapidly as well. Due to technological and spatial constraints, the capacity of mobile power sources has become one of the most important bottlenecks of further development. Moreover, since the power efficiency is a relatively new requirement, there are only a few tools addressing it, and the existing ones have certain drawbacks. The goal of this thesis is to design and implement a tool helping developers of Android mobile applications to identify the battery-hungry parts of application code. The proposed solution is based on a novel approach - examination of power use estimations derived from the device's state together with the information about the code that is currently being executed. The prototype implementation has been tested on two Android applications with the conclusion that the proposed approach can provide useful information to the developer, but in practice, it encounters technical limitations reducing its accuracy, and thus it can not be used as a fully automated tool for identification of battery-hungry parts of Android application code.

OF THE THESIS Name: Bc. Veronika Coufalová, DiS. Specialization: Adictology Head of the thesis: Mgr. Jaroslav Vacek Opponent: Ing., Mgr., Bc. Vendula Běláčková Pages: 83 The name of the thesis: EFFECT OF PARTIAL PROHIBITION ON RESTAURANTS FROM THE PERSPECTIVE OF EMPLOYEES: A QUALITATIVE INVESTIGATION This thesis deals with how the prohibition reflected in the attitudes and behavior of employees and the behavior of consumers in restaurants and bars. The theoretical part is focused on the main substances alcohol and methanol, next on the related topics methanol cases and related facts in criminology. Another chapter is aimed at the prohibition in the country. The research was carried out through qualitative methods of data collection, which was formulated in the form of interviews, which were designed according to the draft of the questionnaire. This research was anonymous, respondents were informed of the ethical rules and the possibility of withdrawing from the research file. Next were used methods expert estimates for research changes in behavior and the method of triangulation. In the research was included 30 restaurants and bars selected nomination technique. Results are evaluated on the basis of data transferred from the audio recording into Excel spreadsheets, where it was possible to insert...

There are many models used to estimate demand elasticities. We present a complex review of these studies in our thesis. Our empirical goal is to compare LES, Translog and QUAIDS demand systems according to their performance. In parallel, we estimate the elasticities of meat demand in the Czech Republic for the period 2010 - 2015 using the data of the household budget survey. Comparing the systems by the Akaike and Schwarz criterion, LES demonstrates the best fit for this kind of data. The average of price elasticity for different kinds of meat in the examined period is -0.99, income elasticity then equals to 1.12. These results can have important implications for tax policy, or for commercial use. JEL Classification F12, F21, F23, H25, H71, H87 Keywords Demand, comparison, LES, Translog, QUAIDS, meat Author's e-mail 55606678@fsv.cuni.cz Supervisor's e-mail milan.scasny@czp.cuni.cz

Master of Arts / Department of Economics / William F. Blankenau / The aim of this paper is to provide an analysis of the consumption, investing, and savings data across racial minority categories within the United States. This paper examines the three biggest minority racial categories within the United States. These three racial categories include Blacks, Asians, and Hispanics. Consumption patterns across minority groups are examined in Chapter 2. These patterns give insights into the annual purchasing decisions of Blacks, Asians, and Hispanics. Many of the choices in budget allocation to specific categories have consequences and implications that are explained at the end of this chapter. Chapter 3 focuses on savings characteristics of minority populations in the United States. 401k plans, IRAs, and other retirement plans are examined for different savings behaviors and rates across minority groups. This paper examines the savings attitudes and survey responses of participants to get a feel of the overall savings climate across racial groups. Chapter 4 examines the investment behaviors and attitudes of different minorities. The financial risk tolerance and portfolio composition of different minority groups are examined to gain insights into the large wealth gap between Whites and minorities in the United States. In addition, this paper examines the hypothesized reasons for the differences between racial categories in consumption, investing, and saving choices. In Chapter 5, I explore the different theories and assumptions presented in the literature on these topics in order to give the reader insight into why racial groups might make different consumption, investment, and savings decisions even when controlling for socioeconomic variables. The final chapter explains the consequences of consumption, investment, and savings decisions for the individual, the community, and the United States.

In his well-known 1972 article, "Famine, Affluence, and Morality," and in subsequent books, Peter Singer argues that we can and should prevent the needless suffering and death of the global poor by giving to foreign aid. This call for charity and assistance is mostly uncontroversial. However, foreign aid's effectiveness is highly contested among the aid literature. As the first part of this paper shows, all aid encounters inefficiencies, with the major problems being diversion of funds and perverse incentives. I look at each of these in turn, concluding that most aid is ineffective and sometimes even harmful—with the possible exception of short-term humanitarian aid. Consideration of aid evaluation and emerging forms of aid like microfinance and unconditional cash transfers provides no solution to aid's problems. In the second part of the paper I consider potentially superior methods for preventing the suffering and death of the global poor. Free trade, open immigration, and increased consumption all seem like promising ways to alleviate poverty and grow developing economies, but only increased consumption of disposable luxury goods fulfills Singer's obligation for the individual to prevent bad things from happening. To show that this idea stands, the rest of the paper will focus on increased consumption's relation to manufacturing, so-called sweatshop labor, economic growth, and alleviation of poverty. Finally, I will address potential limitations and objections to my approach, concluding that consumption of disposable luxury goods is the best way to fulfill Singer's obligation to aid the global poor.

The aim of this thesis is to identify potential routes of participation in environmentally sustainable changes of the Swedish meat production and consumption. Changes are needed as meat production and consumption have been linked to serious environmental problems, such as climate change, biodiversity loss, and land use change. Scientists, international organizations, and Swedish government authorities have identified individual consumer responsibility as key in making that change happen. The public is to be informed and educated to make more environmentally sustainable choices as meat consumers, and become more supportive of policy instruments. This strategy, which mirrors the dominant approach to solving environmental problems, is suggested by government authorities despite their suspicion that media should have made most Swedes aware of the environmental impacts of meat. In this thesis potential participatory routes are identified through the analysis of Swedish news media and audience framings of meat production and consumption. Media framing is studied as an important source of information, and perhaps motivation, crucial in the individualized consumer responsibility approach. The media framing is studied through content analysis of mainstream and alternative radical newspapers. The audiences’ framing of meat may be influenced by media, but also by their everyday experiences, beliefs, values, and opinions. Focus group discussions with reception elements are the methods used for studying how audiences frame meat and use media in the process. The concept of participation is broadened to include passive and active forms to capture in which roles individuals consider to contribute to changing meat production and consumption. It is not self-evident that routes to change must include individual participation, since responsibility may be attributed to other actors, both by media and their audiences. The results imply only participatory route supported by media and audience framing. It is the one that mirrors the individualized consumer responsibility approach to solving environmental problems. The major barrier to the route is the audiences’ perceived inability to act. In an alternative route supported by both media and audience framing, state centered actors are made responsible for enforcing change. Here, the major barrier is the perceived unlikeliness of powerful actors assuming responsibility. Audiences construct no citizen roles for themselves to participate in. Neither does media, who only address audiences as consumers. Based on these findings it is suggested that the outlook for the individualized responsibility approach to making meat production and consumption environmentally sustainable is gloomy. At least if it the approach is to continuously rely on the information and motivation offered by media.