Masters of Science / Sometimes, dental impressions need to be transported to distant laboratories. It has been reported that the temperature in a vehicle can reach up to 66C when the outdoor temperature is 38C. These temperatures may be reached during South African summers. The objective of this in vitro study was to investigate the effect of temperature and time on the dimensional stability of two elastomeric impression materials. Methodology: Specimens consisted of impressions made of an ISO-specified test-block featuring a pattern of grooves. Materials used were polyether (Impregum Penta) and polyvinylsiloxane (Affinis Precious regular body). Using an SLR camera and standardized technique, the specimens were photographed at 2 different temperatures (21°C and 66°C) and 3 time intervals (30min, 8hrs and 14 days). This resulted in a total of 12 groups (n=10) to be compared. Digital images of the impressions were calibrated and measured using digital analyzing software. These distances were used to evaluate the mean % dimensional change (%DC) for each group. VEPAC module of Statistica 10 was used for the statistical analysis. To analyze exactly where the differences lied, a Fisher LSD correction was applied to correct for multiple pair wise comparisons. Results: Comparing polyether with silicone, there was no difference in the mean %DC for specimens kept at 21°C for 8hrs (polyether=0.364; silicone=0.237). Neither was there a difference between polyether and silicone when heated to 66°C, cooled off, and measured after 8hrs (polyether=0.306; silicone=0.297) or after 14 days (polyether=-0.272; silicone=-0.093). For both polyether and silicone, the mean %DC of the groups exposed to 66°C, cooled off and measured after 8hrs (polyether=0.306; silicone=0.297) differed significantly when compared to the group measured after 14 days (polyether=-0.2723; silicone=-0.092) (P<0.0001 and P=0.0029 resp). For both polyether and silicone, the mean %DC of the groups exposed to 66°C, cooled off and measured after 8hrs (polyether=0.306; silicone=0.297) did not differ when compared to the 21°C (polyether=0.364; silicone=0.237). Conclusions: Within limitations of this study, both materials were heat-sensitive. It is recommended that materials return to 21°C before casting. Despite statistical differences, all results were within ISO specifications of maximum 1.5%DC.

No description available.

INTRODUCTION: Ebola virus (EBOV) is a highly infectious and often lethal filovirus that causes hemorrhagic fever, with a reported case fatality rate of 40-90%. There are currently no Food and Drug Administration (FDA) approved medical countermeasures (MCMs) for EBOV. Non-human primates (NHPs) remain the gold standard animal model for EBOV research as they most accurately recapitulate human disease. OBJECTIVE: This study aimed to characterize the temporal viral pathogenesis of EBOV in the liver of infected rhesus macaques using routine histopathology, multiplex immunohistochemistry (mIHC) and multiplex fluorescent In Situ Hybridization (mFISH), refined by digital pathology (DP) and image analysis (DIA). METHODS: 21 FFPE liver sections from EBOV-infected rhesus macaques were examined microscopically (Uninfected controls n=3; 3 DPE n=3; 4 DPE n=3; 5 DPE n=3; 6 DPE n=3; Terminal n=6). Tissues were stained with H&E and PTAH for histopathological scoring. Three serial sections were fluorescently immunolabeled or hybridized under three independent conditions (1.EBOV VP35, Tissue Factor, CD68; 2.EBOV VP35, Heppar, Myeloperoxidase (MPO); 3.EBOV VP35, IL-6, ISG-15). Slides were digitized by a Vectra PolarisTM fluorescent whole slide scanner and DIA was conducted using HaloTM image analysis software. Statistical analysis was conducted using GraphPad PrismTM 8.0. RESULTS: Comparing peracute (3-4 DPE) to acute (5-6 DPE) and terminal (6-8 DPE) EBOV infection, there is a statistically significant (p < 0.05) increase in hepatic inflammation and fibrin thrombi, correlating with an absolute increase in macrophages (CD68), neutrophils (MPO), and total % of Tissue Factor in the liver. There is also a significant increase in the severity of necrosis, which correlates with a decrease in Heppar. While there was significant colocalization of VP35 and CD68 starting at 4 DPE, there was only rare colocalization of VP35 with Heppar, even in terminal animals. Similar to mIHC, progressive and statistically significant differences were observed in gene expression when comparing peracute to acute and terminal EBOV infection. IL-6 predominated within periportal fibrovascular compartments, but also colocalized within cells concurrently expressing EBOV VP35. EBOV VP35 expression was observed within histiocytes, endothelial cells, and less commonly hepatocytes. ISG-15 expression was observed in periportal regions and in proximity to cells expressing EBOV VP35, but colocalization within EBOV VP35 expressing cells was an extremely rare event. CONCLUSION: Qualitative tools are well suited for confirming virulence and viral tissue tropism, but do little to build on our current understanding of disease. Using DIA in partnership with mIHC and mFISH, this study quantified statistically significant temporal changes in the immunoreactivity and hybridization of host and viral biomarkers that have previously been linked to the pathogenesis of EBOV. Taken together, these tools have enabled us to characterize minute changes that reflect magnitudes of biological variability simply not feasible to detect with the human eye. Furthermore, spatial context has refined our current understanding of differential gene expression of EBOV, which has the potential to aid in development of host-directed therapies. The establishment of these benchmarks will serve as a guide for the validation of cross-institutional EBOV animal models.

No description available.

Fluidized bed technology is used for thermal conversion of solid fuels (combustion and gasification) and is especially suitable for conversion of low-rank fuels such as biomass and waste. The performance of fluidized bed units depends on the fuel mixing and fuel-gas contact. Thus, it is important to understand these two phenomena in order to develop models for reliable design and scale up of fluidized bed units. This work investigates, under conditions representative for industrial fluidized bed units, the lateral fuel mixing (in a unit with a cross section of 1.44 m2 both at hot and cold conditions) and the bubble flow distribution (in a 1.2 m-wide 2-dimensional unit). The work confirms previous findings on the formation of preferred bubble paths and shows that these bubble paths are enhanced by lowering the fluidization velocity, increasing the dense bed height and reducing the pressure drop across the gas distributor. From the fuel mixing experiments, an estimation of the lateral effective dispersion coefficient to values in the order of 10-3 m2/s is obtained under both hot and cold conditions. The experiments under cold conditions give additional qualitative information on the fuel mixing patterns such as flotsam/jetsam tendencies. The camera probe developed for fuel tracking under hot conditions enables to study the fuel dispersion under real operation at relevant industrial scales. Based on the characteristics of the bubble path flow, a model for the horizontal fuel dispersion on a macroscopic scale is formulated and shown to be able to give a good description of the experimental data. As opposed to the commonly applied diffusion-type modeling of the lateral solids dispersion, the proposed model facilitates integration with models of the bubble flow. Thus, the present modeling work is a first step to provide a modeling of the fuel dispersion, which uses as inputs only the main operational parameters of the fluidized bed.

Materiais cerâmicos obtidos a partir de pós nanocristalinos permitem a obtenção de excelentes propriedades após sinterização, devido à possibilidade de redução da temperatura final de sinterização com conseqüente refinamento microestrutural e excelente densificação. Nesse contexto, a zircônia tetragonal (ZrO2 (t)) tem um importante papel, pois suas características intrínsecas, tais como a transformação martensítica (ZrO2 (t-m)), permitem o desenvolvimento de excelentes propriedades, destacando-se a elevada tenacidade à fratura e resistência a flexão, tornando-a um material diferenciado visando aplicações nobres onde propriedades mecânicas e confiabilidade sejam pré-requisitos. Visando otimizar estas aplicações, se faz necessário conhecer as correlações entre as propriedades mecânicas e a microestrutura. Sendo assim, é de extrema importância promover a revelação microestrutural desses materiais, de forma padronizada e com representatividade estatística, o que exige cuidados nas técnicas de preparação ceramográfica. Nesse trabalho pretende-se caracterizar microestruturalmente cerâmicas a base de ZrO2(Y2O3) nanométrica, visando estudar os efeitos da temperatura e do tempo de sinterização na cinética de crescimento de grão. Foi definida uma rota de preparação e análise ceramográfica propondo seqüência de lixas e panos de polimento, assim como cargas e tempos em cada etapa. As amostras foram atacadas termicamente e micrografias foram obtidas. As micrografias foram processadas por rotinas de análise digital de imagens, visando definir padronizações para determinação de parâmetros microestruturais de interesse, tais como distribuição de tamanhos de grãos, densidade de grãos por unidade de área, razão de aspecto, etc. Foi estudado o efeito das condições de sinterização (temperatura final e tempo de isoterma) no crescimento de grãos. / Ceramic materials obtained from nanocrystalline powders enable the obtaining of excellent properties after sintering, due to the possibility of reducing the final sintering temperature with consequent microstructural refinement and excellent densification. In this context, tetragonal zirconia (ZrO2 (t)) has an important role, since their intrinsic characteristics, such as the martensitic transformation (ZrO2 (t-m)), allow the development of excellent properties, highlighting the high tenacity to fracture and resistance to bending, making it a differentiated material aiming noble applications where mechanical properties and reliability are prerequirements. In order to optimize these applications, it is necessary to know the correlations between the mechanical properties and microstructure. Thus, it is extremely important to promote the microstructural disclosure of these materials, in a standardized manner and with statistical representativeness, which requires care with the ceramographic preparation techniques. This work aims to microstructurally characterize ceramics based on nanometric ZrO2(Y2O3), looking for the study of the effects of the sintering temperature and time on the graingrowth kinetics. It was defined a route for ceramographic preparation and analysis proposing a sequence of sandpapers and polishing cloths, as well as loads and times at each stage. The samples were thermally etched and micrographs were obtained. The micrographs were processed through routines of digital image analysis, aiming the definition of standards for the determination of microstructural parameters of interest, such as distribution of grain sizes, density of grains per unit of area, aspect ration, and others. It was studied the effects of the sintering conditions (final temperature and time of isotherm) on the grain growth.

Materiais cerâmicos obtidos a partir de pós nanocristalinos permitem a obtenção de excelentes propriedades após sinterização, devido à possibilidade de redução da temperatura final de sinterização com conseqüente refinamento microestrutural e excelente densificação. Nesse contexto, a zircônia tetragonal (ZrO2 (t)) tem um importante papel, pois suas características intrínsecas, tais como a transformação martensítica (ZrO2 (t-m)), permitem o desenvolvimento de excelentes propriedades, destacando-se a elevada tenacidade à fratura e resistência a flexão, tornando-a um material diferenciado visando aplicações nobres onde propriedades mecânicas e confiabilidade sejam pré-requisitos. Visando otimizar estas aplicações, se faz necessário conhecer as correlações entre as propriedades mecânicas e a microestrutura. Sendo assim, é de extrema importância promover a revelação microestrutural desses materiais, de forma padronizada e com representatividade estatística, o que exige cuidados nas técnicas de preparação ceramográfica. Nesse trabalho pretende-se caracterizar microestruturalmente cerâmicas a base de ZrO2(Y2O3) nanométrica, visando estudar os efeitos da temperatura e do tempo de sinterização na cinética de crescimento de grão. Foi definida uma rota de preparação e análise ceramográfica propondo seqüência de lixas e panos de polimento, assim como cargas e tempos em cada etapa. As amostras foram atacadas termicamente e micrografias foram obtidas. As micrografias foram processadas por rotinas de análise digital de imagens, visando definir padronizações para determinação de parâmetros microestruturais de interesse, tais como distribuição de tamanhos de grãos, densidade de grãos por unidade de área, razão de aspecto, etc. Foi estudado o efeito das condições de sinterização (temperatura final e tempo de isoterma) no crescimento de grãos. / Ceramic materials obtained from nanocrystalline powders enable the obtaining of excellent properties after sintering, due to the possibility of reducing the final sintering temperature with consequent microstructural refinement and excellent densification. In this context, tetragonal zirconia (ZrO2 (t)) has an important role, since their intrinsic characteristics, such as the martensitic transformation (ZrO2 (t-m)), allow the development of excellent properties, highlighting the high tenacity to fracture and resistance to bending, making it a differentiated material aiming noble applications where mechanical properties and reliability are prerequirements. In order to optimize these applications, it is necessary to know the correlations between the mechanical properties and microstructure. Thus, it is extremely important to promote the microstructural disclosure of these materials, in a standardized manner and with statistical representativeness, which requires care with the ceramographic preparation techniques. This work aims to microstructurally characterize ceramics based on nanometric ZrO2(Y2O3), looking for the study of the effects of the sintering temperature and time on the graingrowth kinetics. It was defined a route for ceramographic preparation and analysis proposing a sequence of sandpapers and polishing cloths, as well as loads and times at each stage. The samples were thermally etched and micrographs were obtained. The micrographs were processed through routines of digital image analysis, aiming the definition of standards for the determination of microstructural parameters of interest, such as distribution of grain sizes, density of grains per unit of area, aspect ration, and others. It was studied the effects of the sintering conditions (final temperature and time of isotherm) on the grain growth.

Fluidization of a granular assembly of solid particles is a process where particles are suspended in a fluid by the upward flow of fluid through the bed. This process is important in industry as it has a wide range of applications due to the high mixing and mass transfer rates present as a result of the rapid movement of particles which occurs in the bed. The dynamics of fluidization is heavily dependent on the particle scale physics and the forces acting at a particle level. For particles with sizes and densities less than 100μm and 103 kg/m3, the importance of interparticle forces such as cohesion to the fluidization phenomena observed increases compared to larger particles where phenomena observed are more dependent on hydrodynamic forces. These smaller sized particles are increasingly in high demand in industrial processes due to the increasing surface area per unit volume obtained by decreasing the particle size. Decreasing particle however leads to an increase in the impact of cohesive interparticle forces present between particles thus altering fluidization phenomena. It is thus necessary to get a greater understanding of how these cohesive forces alter fluidization behaviour both at the particle and also at the bulk scale. This work begins with an experimental study of a fluidized bed using high speed imaging. The applicability of particle image velocimetry for a dense bed is examined with verification and validation studies showing that particle image velocimetry is able to accurately capture averaged velocity profiles for particles at the front wall. A digital image analysis algorithm which is capable of accurately extracting particle solid fraction data for a dense bed at non-optimum lighting conditions was also developed. Together both experimental techniques were used to extract averaged particle mass flux data capable of accurately capturing and probing fluidization phenomena for a dense fluidized bed. This simulation studies carried out for this work looks to examine the impact of cohesive forces introduced using a van der waal cohesion model on phenomena observed at different length scales using DEM-CFD simulations. Numerical simulations were run for Geldart A sized particles at different cohesion levels represented by the bond number and at different inlet gas velocities encompassing the different regimes fluidization regimes present. A stress analysis was used to examine the mechanical state of the expanded bed at different cohesion levels with the vertical component of the total stress showing negative tensile stresses observed at the center of the bed. Further analysis of the contact and cohesive components of the stress together with a kcore and microstructural analysis focusing on the solid fraction and coordination number profiles indicated that this negative total stress was caused by a decrease in the contact stress due to breakage of mechanical contacts as cohesive forces are introduced and increased. A pressure overshoot analysis was also conducted with the magnitude of the overshoot in pressure seen during the pressure drop analysis of a cohesive bed shown to be of equivalent magnitude to the gradient of the total negative stress profile. The in-homogeneous nature of the bed was probed with the focus on how introducing cohesion levels increase the degree of inhomogeneity present in the expanded bed and how local mesoscopic structures change with cohesion and gas velocity. It was shown that increasing cohesion increases the degree of inhomogeneity in the bed as well as increasing the degree of clustering between particles. A majority of particles were shown to be present in a single macroscopic cluster in the mechanical network with distinct local mesoscopic structures forming within the macroscopic cluster. The cohesive bed also expanded as distinct dense regions with low mechanical contact zones in between these regions. A macroscopic cluster analysis showed that the majority of particles are in strong enduring mechanical and cohesive contact. Increasing cohesive forces were also shown to not only create a cohesive support network around the mechanical network but also strengthen the mechanical contact network as well. The significance of the strong and weak mechanical and cohesive forces on fluidization phenomena was also examined with analysis showing that the weak mechanical forces act to support the weak mechanical forces. The cohesive force network however was non coherent with strong forces significantly greater than weak forces. Fluidization phenomena was shown to be driven by the magnitude of the strong cohesive forces set by the minimum particle cutoff distance. This also called into question the significance of the cohesive coordination number which is dependent on the maximum cohesive cutoff. The value of the maximum cutoff was shown to be less significant as no significant changes were observed in the stress and microstructure data as the maximum cutoff was altered. Simulations with different ratios of cohesive and non cohesive particles were also undertaken and showed that a disruption in the cohesive force network leads to changes in the stress state and microstructure of the bed thus changing the fluidization phenomena observed at all length scales. The nature of the strong cohesive force network thus drives fluidization phenomena seen in the bed.

This dissertation investigates microstructure/property relations in ultrahigh carbon steel (UHCS) with the aim of improving toughness while retaining high hardness. Due to high C contents (ranging from 1 to 2 wt%), UHCS exhibit high strength, hardness, and wear resistance. Despite this, applications for UHCS are currently limited because they typically contain a continuous network of proeutectoid cementite that greatly reduces ductility and toughness. In previous research, thermomechanic processing had seen considerable success in breaking up the network. However, the processing is difficult and has thus far seen very limited industrial application. Chemical modification of the steel composition has also seen some success in network break-up, but is still not well understood. There have been relatively few fundamental studies of microstructure evolution in UHCS; studies in the literature typically focused on lower C steels (up to 1 wt% C) or on cast irons (>2.1 wt% C). Thus, this work was undertaken to gain a better understanding of microstructural changes that occur during heat treatment and/or chemical modification of UHCS with a focus on the distribution of proeutectoid cementite within the microstructure. This dissertation is composed of eight chapters. The first chapter presents an introduction to phases found in UHCS, descriptions of research materials used in each chapter, and the hypotheses and objectives guiding the work. The second chapter describes a study of the microstructure found in a 2C-4Cr UHCS before and after an industrial-scale austenitizating heat treatment that increased hardness and toughness and also produced discrete carbide particles in the matrix. The third chapter establishes and demonstrates a metric for measuring connectivity in carbide networks. The fourth chapter describes a series of heat treatments designed to investigate kinetics of spheroidization and coarsening of carbide particles and denuded zones near cementite network branches in 2C-4Cr UHCS. The fifth chapter describes an additional series of heat treatments comparing coarsening kinetics in 2C-1Cr and 2C-4Cr UHCS. Lowering the Cr content caused clustering of cementite particles near grain boundaries, in contrast to the denuded zones observed in the higher Cr UHCS. The fifth chapter details four in situ confocal laser scanning microscopy heat treatments of 2C-4Cr UHCS. The seventh chapter investigates the effects of a 2wt% Nb addition on 2C-4Cr UHCS. The eighth and final chapter summarizes the findings of all the experiments of the previous chapters and revisits the objectives and conclusions.

Laser Doppler velocimetry (LDV) measurements and hydrogen-bubble flow-visualization techniques were used to examine the near-wall flow structure of 2-D and 3-D turbulent boundary layers (TBLs) over a range of low Reynolds numbers. The goals of this research were (1) an increased understanding of the flow physics in the near wall region of turbulent boundary layers, (2) to observe and quantify differences between 2-D and 3-D TBL flow structures, and (3) to document Reynolds number effects for 3-D TBLs. An ultimate application of this work would be to improve turbulence modeling for 3-D flows. The LDV data have provided results detailing the turbulence structure of the 2-D and 3-D TBLs, as well as low uncertainty skin friction estimates. These results include mean Reynolds stress distributions, flow skewing results, and U and V spectra. Effects of Reynolds number for the 3-D flow were examined when possible. Comparison to results with the same 3-D flow geometry but at a significantly higher Reynolds number provided unique insight into the structure of 3-D TBLs. While the 3-D mean and fluctuating velocities were found to be highly dependent on Reynolds number, a previously defined shear stress parameter was discovered to be invariant with Reynolds number. The hydrogen-bubble technique was used as a flow-visualization tool to examine the near-wall flow structure of 2-D and 3-D TBLs. Both the quantitative and qualitative results displayed larger turbulent fluctuations with more highly concentrated vorticity regions for the 2-D flow. The 2-D low-speed streaky structures experienced greater interaction with the outer region high-momentum fluid than observed for the 3-D flow. The near-wall 3-D flow structures were generally more quiescent. Numerical parameters quantified the observed differences, and characterized the low-speed streak and high-speed sweep events. All observations indicated a more stable near-wall flow structure with less turbulent interactions occurring between the inner and log regions for a 3-D TBL. / Ph. D.