Spelling suggestions: "subject:"[een] HYDROGEN PEROXIDE"" "subject:"[enn] HYDROGEN PEROXIDE""
41 |
ELECTROPHYSIOLOGICAL COMPARISON OF NaV1.5 EXPRESSED IN HEK293 CELLS TO NATIVE NaV CURRENTS IN CARDIAC MYOCYTESVALINSKY, WILLIAM COREY 22 August 2011 (has links)
Contraction of cardiac muscle is a highly regulated event that relies on a delicate balance of ions entering and leaving the cell through ion channels. In particular, voltage gated sodium channels are responsible for the rapid depolarization that leads to a contraction. During an oxidative challenge, sodium channels rapidly activate, but do not fully turn off. This alters the rate of cardiac repolarization and can induce cardiac arrhythmias. It is currently unknown whether the most common sodium channel isoform found in the heart, NaV1.5, generates this oxidant-induced persistent current or if other isoforms are responsible. Therefore, I sought to further explore the biophysical properties NaV1.5, and determine if it can enter this persistent mode.
I tested the biophysical properties of native INa in cardiac myocytes and in NaV1.5 transfected HEK293 cells under macro cell-attached voltage-clamp. I used a sodium channel enhancer (Anemonia sulcata toxin II; 10 nM), a sodium channel blocker (tetrodotoxin; 10 nM) and a model of oxidative stress (H2O2; 100 µM, 200 µM, 1000 µM) to compare and contrast the cellular responses between both cell types. I observed that transfected HEK293 cells and cardiac myocytes were unaffected by H2O2 at various concentrations. Given the lack of other isoforms in transfected HEK293 cells, and the low abundance (<5%) of other isoforms in cardiac myocytes, I propose that NaV1.5 function is unaffected by H2O2. Furthermore, ATX II prolonged the inactivation process in both HEK293 cells and cardiac myocytes in a voltage-dependent manner, indicating that NaV1.5 can give rise to persistent sodium current. Finally, by comparing both cell types under control settings, I found that transfected HEK293 cells inactivated at a much slower rate and at more negative potentials compared to the current in cardiac myocytes. My results suggest that NaV1.5 does not underlie oxidant-induced persistent current and that β subunits likely play a significant role in the inactivation process. / Thesis (Master, Physiology) -- Queen's University, 2011-08-19 14:46:42.665
|
42 |
Aromatic alcohol oxidase, aldehyde oxidase and mannitol oxidase in terrestrial gastropodsLarge, Andrew January 1995 (has links)
No description available.
|
43 |
The performance of potassium permanganate and hydrogen peroxide oxidation and/or alum coagulation in the removal of complexed FE(II) from drinking water /Bellamy, Julia Davidson, January 1992 (has links)
Thesis (M.S.)--Virginia Polytechnic Institute and State University, 1992. / Vita. Abstract. Includes bibliographical references (leaves 123-127). Also available via the Internet.
|
44 |
Chemical approaches to probe environmental stress in ArchaeaTarlykov, Pavel Victorovich. January 2009 (has links) (PDF)
Thesis (MS)--Montana State University--Bozeman, 2009. / Typescript. Chairperson, Graduate Committee: Brian Bothner. Includes bibliographical references (leaves 63-71).
|
45 |
Antimicrobial and spermicidal activity of hydrogen peroxide and nonoxynol-9 based gel preparationsGrimmett, Paul E. January 2004 (has links)
Thesis (M.S.)--Marshall University, 2004. / Title from document title page. Document formatted into pages; contains x, 67 p. Includes abstract. Includes bibliographical references (p. 48-50).
|
46 |
The catalytic decomposition of hydrogen peroxide by aluminum oxyiodide hydrosols ...Cohen, Benjamin, January 1936 (has links)
Thesis (Ph. D.)--Columbia University, 1936. / Vita. Bibliography: p. 33.
|
47 |
The effect of boar spermatozoa senility on the peroxidation of sperm cell lipidsMrotek, James Joseph, January 1965 (has links)
Thesis (M.S.)--University of Wisconsin--Madison, 1965. / eContent provider-neutral record in process. Description based on print version record. Bibliography: l. 26-29.
|
48 |
Hydrogen-peroxide catalase treatment of coagulase-positive staphylococci in milkAmin, Virendrakumar Manibhai. January 1968 (has links)
Thesis (Ph. D.)--University of Wisconsin--Madison, 1968. / Typescript. Vita. Includes bibliographical references.
|
49 |
The absorption spectrum and photochemical dissociation of hydrogen peroxide ...Dawsey, Lynn Hugh, January 1929 (has links)
Thesis (Ph. D.)--Johns Hopkins University, 1929. / Biography. Description based on print version record.
|
50 |
An exploratory study of using hydrogen peroxide as oxygen source in aerobic upflow sludge blanket reactor /Poon, Wing Chi. January 2005 (has links)
Thesis (M.Phil.)--Hong Kong University of Science and Technology, 2005. / Includes bibliographical references (leaves 114-120). Also available in electronic version.
|
Page generated in 0.0369 seconds