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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
1

P53 AND REACTIVE OXYGEN SPECIES: A CONVOLUTED STORY

Liu, Bin 01 January 2007 (has links)
The tumor suppressor p53 has a close relation with reactive oxygen species (ROS). As an indispensable component of the cellular redox system, ROS not only have been established to be involved in p53-dependent apoptosis, but also regulate p53 activity. Recent studies revealed several novel actions of p53, such as transactivation of antioxidative proteins, mitochondria translocation and inhibition of glycolysis. The fate of cells where p53 signaling pathways are initiated is either survival or death. In this review, we examine the hypothesis that ROS regulate cell fate through p53, in a way that physiological ROS levels trigger the protective pathways, while p53 behaves more like a cell killer under cytotoxic oxidative stress.
2

Invadolysin, a conserved lipid droplet-associated protease interacts with mitochondrial ATP synthase and regulates mitochondrial metabolism in Drosophila

Duca, Edward January 2011 (has links)
Invadolysin (inv) is a member of the M8 class of zinc-metalloproteases and is conserved throughout metazoans. It is essential for development and invadolysin homozygous Drosophila mutants are third instar larval lethal. These larvae exhibit a reduced larval brain size and an absence of imaginal discs. Detailed analysis showed that inv mutants exhibit pleiotropic effects, including defects with chromosome architecture, cell cycle progression, spindle assembly, nuclear envelope dynamics, protein turnover and problems with germ cell migration. These findings indicated that Invadolysin must have a critical role in Drosophila. In order to better understand these roles, I set out to identify genetic interactors of invadolysin. I performed a genetic screen scoring for enhancer/suppressor modification of a ‘rough eye’ phenotype induced by invadolysin overexpression. Screening against the Drosdel ‘deficiency kit’ identified numerous genetic interactors including genes linked to energy regulation, glucose and fatty acid pathways. Immunofluorescence experiments in cultured cells showed that H. sapiens Invadolysin localises to the surface of lipid droplets (LD), and subcellular fractionation confirmed its enrichment to these structures. Lipid droplets are highly dynamic organelles involved not only in energy storage but also in protein sequestration, protein and membrane trafficking, and cell signaling. Drosophila fat bodies are enriched in LDs and therefore important energy stores. In addition, they are nutritional sensors and regulators, which are proposed to be the ortholog of vertebrate liver and adipose tissue. Mutant inv fat bodies appeared smaller and thinner than wild type fat body, and accumulated lower levels of triacylgylcerides. This indicated that the loss of invadolysin might be affecting lipid metabolism and storage, confirming the genetic data. However, it was not clear whether these effects were due to the direct action of Invadolysin. Hence, transgenic fly lines expressing either HA, RFP or FLAG tagged forms of Invadolysin were generated to identify physical interactors of Invadolysin. Subsequent mass spectrometry analysis detected ATP synthase-α, -β and -d as interactors. This result suggested that Invadolysin might play a role in regulating mitochondrial function, which might then be manifest in the fat body as the defects previously observed. Energy levels are known to affect the cell cycle, cell growth, lipid metabolism and inevitably development. Further in vivo and in vitro experiments confirmed this hypothesis. Genetic crosses confirmed the interaction of invadolysin with ATP-synthase subunit-α, whilst staining of mitochondria in mutant third instar larval fat bodies suggested decreased mitochondrial activity. Mutants also showed lower ATP levels and an accumulation of reactive oxygen species, hence indicating the possibility of a dysfunctional electron transport chain. Lipid droplets are known to interact with mitochondria, whilst ATP synthase has been found on lipid droplets by proteomic studies in Drosophila. Therefore, based on these data, we propose that Invadolysin is found, with ATP synthase, on lipid droplets, where Invadolysin (likely acting as a protease) could be aiding the normal processing or assembly of ATP synthase. This interaction is vital for the proper functioning of ATP synthase, and hence mitochondria. In this scenario, cellular ATP needs are not met, energy levels drop which results in an inhibition of fatty acid synthesis, cell and organismal growth defects.
3

Hypoxia-induced pulmonary hypertension in type 2 diabetic mice

Pan, Minglin, Han, Ying, Si, Rui, Guo, Rui, Desai, Ankit, Makino, Ayako 02 1900 (has links)
Hypoxia-induced pulmonary hypertension (HPH) is a progressive disease that is mainly caused by chronic exposure to high altitude, chronic obstructive lung disease, and obstructive sleep apnea. The increased pulmonary vascular resistance and increased pulmonary arterial pressure result in increased right ventricular afterload, leading to right heart failure and increased morbidity. There are several clinical reports suggesting a link between PH and diabetes, insulin resistance, or obesity; however, it is unclear whether HPH is associated with diabetes as a progressive complication in diabetes. The major goal of this study is to examine the effect of diabetic ''preconditioning'' or priming effect on the progression of HPH and define the molecular mechanisms that explain the link between diabetes and HPH. Our data show that HPH is significantly enhanced in diabetic mice, while endothelium-dependent relaxation in pulmonary arteries is significantly attenuated in chronically hypoxic diabetic mice (DH). In addition, we demonstrate that mouse pulmonary endothelial cells (MPECs) isolated from DH mice exhibit a significant increase in mitochondrial reactive oxygen species (ROS) concentration and decreased SOD2 protein expression. Finally, scavenging mitochondrial ROS by mitoTempol restores endothelium-dependent relaxation in pulmonary arteries that is attenuated in DH mice. These data suggest that excessive mitochondrial ROS production in diabetic MPECs leads to the development of severe HPH in diabetic mice exposed to hypoxia.
4

Efeito da cisplatina na função, estresse oxidativo e estado redox mitocondrial renal em ratos: efeito protetor da dimetiltiouréia / ?Effect of cisplatin on the function, oxidative stress and renal mitochondrial redox state

Santos, Neife Aparecida Guinaim dos 11 December 2006 (has links)
Embora a cisplatina (cis-diaminocloroplatina II) seja um efetivo agente anticâncer, seu uso clínico é altamente limitado, predominantemente devido ao seu potencial nefrotóxico. Muitos estudos têm demonstrado que a cisplatina causa disfunção mitocondrial em células epiteliais renais e danos ao DNA nuclear devido à ação de espécies reativas de oxigênio tais como superóxido e radicais hidroxila. O aumento na produção destas espécies de oxigênio causa liberação de citocromo c no citosol, iniciando uma cascata de eventos que leva à morte celular por apoptose. A proteção seletiva da mitocôndria contra espécies reativas de oxigênio geradas pela cisplatina nos tecidos intactos tais como os rins, é fundamental na quimioterapia de pacientes com câncer. O presente estudo investigou os efeitos da cisplatina na bioenergética, no estado redox e no estresse oxidativo mitocondrial renal, bem como o potencial protetor da dimetiltiouréia (DMTU), um antioxidante seqüestrador de radicais hidroxila, com relação à toxicidade renal induzida pela cisplatina. Método: Ratos Wistar machos adultos pesando de 200 a 220 g foram divididos em quatro grupos de 8 animais cada. Ao primeiro grupo foi administrada cisplatina (10 mg/ kg) por via intra peritonial (i.p.). O segundo grupo recebeu somente injeções de DMTU (500 mg/kg, i.p., seguida de 2 injeções diárias de 125 mg/Kg, i.p). O terceiro grupo de animais foi tratado com DMTU (500 mg/kg, i.p.), imediatamente antes da injeção de cisplatina (10 mg/kg, i.p.), seguida de 2 injeções diárias de DMTU (125 mg/Kg, i.p.). O grupo controle recebeu somente solução salina (1ml/200g, i.p.). Os animais foram sacrificados 72 horas após a injeção de cisplatina (ou salina). Resultados: O tratamento com a cisplatina resultou em uma marcante diminuição da função renal demonstrada pela elevação dos níveis plasmáticos de uréia e de creatinina, concomitante a uma significativa alteração nos parâmetros relacionados à função Resumo ix mitocondrial (síntese de ATP, estado 3 da respiração, RCR, ADP/O, potencial de membrana e transporte de cálcio); ao estresse oxidativo mitocondrial (oxidação da cardiolipina, atividade da aconitase, lipoperoxidação, níveis de proteína carbonila e proteína sulfidrila); ao estado redox mitocondrial (oxidação do NAD(P)H, relação glutationa reduzida e glutationa oxidada) e à apoptose (atividade da caspase 3). O pré-tratamento dos animais com DMTU preveniu a falência renal aguda e as alterações dos parâmetros mitocondriais , sendo capaz de inibir a morte celular por apoptose. Conclusão: Os resultados demonstram o papel central da mitocôndria na falência renal aguda induzida pela cisplatina, bem como o efeito protetor do DMTU e sugerem que o desenvolvimento de potentes seqüestradores de radicais hidroxila, passíveis de uso clínico, poderia contribuir de forma marcante na prevenção dos danos renais resultantes da quimioterapia com este fármaco. / Although cis-diamminedichloroplatinum (II) (cisplatin) is an effective anticancer agent, its clinical use is highly limited predominantly due to its adverse effects on renal functions. Many studies have shown that cisplatin causes mitochondrial dysfunction and direct injury to nuclear DNA by generating reactive oxygen species such as superoxide and hydroxyl radicals. Overproduction of reactive oxygen species causes the release of cytochrome c into cytosol, thereby triggering the sequence of events leading to cell death via apoptosis. The selective protection of mitochondria against reactive oxygen species generated by cisplatin in intact tissues, such as kidney, is of critical importance in the chemotherapy of patients with cancer. The present study examined the effects of cisplatin on renal mitochondrial bioenergetics, redox state and oxidative stress as well as the protective potential of dimethylthiourea (DMTU), a hydroxyl radical scavenger, against the cisplatin-induced nephrotoxicity. Methods: Adult male Wistar rats weighing 200 to 220g were divided into 4 groups with 8 animals each.. The first group was given a single intraperitoneal (i.p.) injection of cisplatin (10 mg/kg). The second group was given only DMTU (500 mg/kg body weight, i.p, followed by intraperitoneal injections of 125 mg/Kg twice a day until sacrifice). A third group of animals was given DMTU (500 mg/kg body weight, i.p.), just before cisplatin injection (10 mg/kg body weight, i.p.), followed by intraperitoneal injections of DMTU (125 mg/Kg body weight) twice a day until sacrifice. The control group was treated only with saline solution (1ml/200g body weight, i.p.). Animals were sacrificed 72 hours after the treatment. Results: Cisplatin treated animals presented a marked impairment of the renal function evidenced by the elevation of plasmatic creatinine and urea levels simultaneously to a significant alteration of the parameters related to: (a) the mitochondrial function assessed by ATP synthesis, Summary xi state 3 respiration, RCR, ADP/O ratio, membrane potential, calcium uptake; (b) the mitochondrial oxidative stress assessed by cardiolipin oxidation, aconitase activity, lipid peroxidation, protein carbonyls and protein sulphydryl; (c) the mitochondrial redox state assessed by NADPH/NADP+ ratio, GSH/GSSG ratio and (d) apoptosis assessed by caspase-3 activity. DMTU substantially inhibited cisplatin-induced mitochondrial injury and cellular death by apoptosis, thereby suppressing the occurrence of acute renal failure. Conclusions: Results show the central role of the mitochondria in the cisplatin-induced renal acute failure, the protective potential of DMTU and suggest that the development of potent hydroxyl radical scavengers suitable for use in man could minimize the adverse effects of cisplatin in the kidney of patients under chemotherapy.
5

Analysis of metallothionein gene expression in oxidative stress related disorders / by Boitumelo Semete

Semete, Boitumelo January 2004 (has links)
Increased reactive oxygen species (ROS) have been reported to be at the centre of various diseases. Although several reports have implicated elevated levels of ROS in the pathogenesis of diabetes mellitus, the early detection of ROS is still not attainable. This limitation causes difficulty in the early diagnosis of ROS related disorders. The presence of high levels of ROS was reported to result in differential expression of antioxidant genes involved in protecting cells from their deleterious effects. Among the antioxidant genes that are expressed, it was postulated that expression of metallothioneins (MTs) are also induced. MTs are low molecular weight, cysteine-rich proteins involved in metal homeostasis and reported to harbour antioxidant function. The aim of this investigation was to explore MTs as biomarkers for elevated levels of ROS in whole blood of type 2 diabetic (T2D) individuals. The level of ROS in diabetic, non-diabetic as well as individuals at risk of developing T2D was determined via the use of biochemical assays. Real-Time PCR was utilised to analyse the expression of MTs and the presence of MT proteins was analysed via the ELISA. In this study it was observed that diabetic individuals had elevated levels of ROS. However, no significant difference in the expression of MTs and the presence of MT proteins between the diabetic and non-diabetic individuals was observed. In vitro experimental conditions indicated that MT expression is induced by elevated levels of ROS. In pathological conditions the ROS-dependent induction of MT expression needs to be elucidated further. It therefore can be suggested that MTs can not yet be utilised as biomarkers for the detection of elevated levels of ROS in pathological conditions with ROS aetiology. This investigation also highlights the fact that blood is not an optimal medium in which this objective can be attained. / Thesis (Ph.D. (Biochemistry))--North-West University, Potchefstroom Campus, 2005.
6

Exploring the role of 4-hydroxy-2-nonenal and mitochondrial dysfunction in diabetic neuropathy

Akude, Eli Kwaku 07 March 2011 (has links)
In diabetes hyperglycemia and lack of insulin signaling are key factors in the induction of diabetic sensory neuropathy. The combination of these factors in diabetes may enhance oxidative stress and trigger distal nerve damage in the peripheral nervous system. The link between elevated reactive oxygen species (ROS) levels and nerve degeneration is not clear. We tested the hypothesis that elevation of 4-hydroxy-2-nonenal (4-HNE) induced by oxidative stress in diabetes impairs mitochondrial activity and axonal regeneration in dorsal root ganglion (DRG) neurons. Also, we investigated the association between mitochondrial dysfunction and altered mitochondrial proteome in the axons of streptozotocin–induced diabetic rats. Research design and methods. Cultured adult rat DRG sensory neurons were treated exogenously with 4-HNE, and cell survival, axonal morphology, and level of axon outgrowth assessed. Western blot and fluorescence imaging were used to determine changes in the levels of adducts of 4-HNE and abnormalities in the mitochondria. Proteomic analysis using stable isotope labeling with amino acids in cell culture (SILAC) determined expression of proteins in the mitochondria. Results. 4-HNE impaired axonal regeneration, mitochondrial activity and induced aberrant axonal structures along the axons, which mimicked axon pathology observed in nerve isolated from diabetic rats and replicated aspects of neurodegeneration observed in human diabetic neuropathy. Proteins associated with mitochondrial dysfunction, oxidative phosphorylation and biosynthesis were down regulated in diabetic samples. The axons of diabetic neurons exhibited oxidative stress and depolarized mitochondria. CNTF and resveratrol reversed abnormalities in the mitochondrial membrane potential induced by diabetes and treatment of neurons with 4-HNE. CONCLUSIONS. Elevation of 4-HNE levels in diabetes was associated with impaired mitochondrial function and might be an important link between increased ROS levels and nerve degeneration in diabetic neuropathy. Abnormal mitochondrial function correlated with a down-regulation of mitochondrial proteins, with components of the respiratory chain targeted in lumbar DRG in diabetes. The reduced activity of the respiratory chain was associated with diminished superoxide generation within the mitochondrial matrix and did not contribute to oxidative stress in axons of diabetic neurons.
7

TARGETING THE METAL CHELATOR D-PENICILLAMINE TO EXPLOIT THE ELEVATED COPPER AND OXIDATIVE STRESS ASSOCIATED WITH CANCER

Gupte, Anshul 01 January 2008 (has links)
The significantly increased copper and oxidative stress levels are characteristic hallmarks of cancer cells. These differences provide a unique opportunity for selective targeting of cancer cells. D-penicillamine (D-pen) has been proposed to generate reactive oxygen species (ROS) in presence of copper. Therefore, these studies were aimed at investigating the potential application of a currently marketed copper chelator, D-pen, as a novel cytotoxic anti-cancer agent. D-pen was shown to produce ROS, specifically hydrogen peroxide (H2O2), in the presence of cupric sulfate through a copper catalyzed oxidation reaction. During this process D-pen was converted to D-pen disulfide. The experimental proof of the H2O2 generation was conclusively shown with the aid of a novel High Performance Liquid Chromatography (HPLC) assay. The in-vitro cytotoxicity of D-pen co-incubated with cupric sulfate was examined in human beast cancer (MCF-7 and BT474) and leukemia cells (HL-60, HL-60/VCR, and HL-60/ADR). D-pen was shown to cause concentration dependent cytotoxicity in both leukemia and breast cancer cells. A direct correlation between the detection of intracellular ROS and cytotoxicity was established. The treatment of D-pen plus cupric sulfate resulted in a significant reduction in the intracellular thiol content. D-pen is highly hydrophilic and is rapidly eliminated from the body; therefore to improve the intracellular uptake and to protect the thiol group of D-pen, we carried out the synthesis and the in-vitro characterization of a novel gelatin-D-pen conjugate. It was shown that D-pen alone does not enter cells. Confocal microscopy was employed to exhibit the uptake of the novel gelatin-D-pen conjugate by cancer cells. As the cancer cells in-vitro do not accumulate the same levels of copper as reported for cancer cells in-vivo, cancer cells were pre-treated with cupric sulfate to simulate the elevated copper levels. The cupric sulfate pretreatment resulted in reduced thiol level and significantly increased cellular copper content compared to untreated cells. Whereas both free D-pen and gelatin-D-pen conjugate lacked cytotoxicity in un-treated cells, both agents caused concentration dependent cytotoxicity in cupric sulfate pre-treated leukemia cells. Therefore, it was shown that the administration of D-pen as polymer conjugate would potentially provide cytotoxicity and specificity in the treatment of cancer.
8

Analysis of metallothionein gene expression in oxidative stress related disorders / by Boitumelo Semete

Semete, Boitumelo January 2004 (has links)
Increased reactive oxygen species (ROS) have been reported to be at the centre of various diseases. Although several reports have implicated elevated levels of ROS in the pathogenesis of diabetes mellitus, the early detection of ROS is still not attainable. This limitation causes difficulty in the early diagnosis of ROS related disorders. The presence of high levels of ROS was reported to result in differential expression of antioxidant genes involved in protecting cells from their deleterious effects. Among the antioxidant genes that are expressed, it was postulated that expression of metallothioneins (MTs) are also induced. MTs are low molecular weight, cysteine-rich proteins involved in metal homeostasis and reported to harbour antioxidant function. The aim of this investigation was to explore MTs as biomarkers for elevated levels of ROS in whole blood of type 2 diabetic (T2D) individuals. The level of ROS in diabetic, non-diabetic as well as individuals at risk of developing T2D was determined via the use of biochemical assays. Real-Time PCR was utilised to analyse the expression of MTs and the presence of MT proteins was analysed via the ELISA. In this study it was observed that diabetic individuals had elevated levels of ROS. However, no significant difference in the expression of MTs and the presence of MT proteins between the diabetic and non-diabetic individuals was observed. In vitro experimental conditions indicated that MT expression is induced by elevated levels of ROS. In pathological conditions the ROS-dependent induction of MT expression needs to be elucidated further. It therefore can be suggested that MTs can not yet be utilised as biomarkers for the detection of elevated levels of ROS in pathological conditions with ROS aetiology. This investigation also highlights the fact that blood is not an optimal medium in which this objective can be attained. / Thesis (Ph.D. (Biochemistry))--North-West University, Potchefstroom Campus, 2005.
9

Exploring the role of 4-hydroxy-2-nonenal and mitochondrial dysfunction in diabetic neuropathy

Akude, Eli Kwaku 07 March 2011 (has links)
In diabetes hyperglycemia and lack of insulin signaling are key factors in the induction of diabetic sensory neuropathy. The combination of these factors in diabetes may enhance oxidative stress and trigger distal nerve damage in the peripheral nervous system. The link between elevated reactive oxygen species (ROS) levels and nerve degeneration is not clear. We tested the hypothesis that elevation of 4-hydroxy-2-nonenal (4-HNE) induced by oxidative stress in diabetes impairs mitochondrial activity and axonal regeneration in dorsal root ganglion (DRG) neurons. Also, we investigated the association between mitochondrial dysfunction and altered mitochondrial proteome in the axons of streptozotocin–induced diabetic rats. Research design and methods. Cultured adult rat DRG sensory neurons were treated exogenously with 4-HNE, and cell survival, axonal morphology, and level of axon outgrowth assessed. Western blot and fluorescence imaging were used to determine changes in the levels of adducts of 4-HNE and abnormalities in the mitochondria. Proteomic analysis using stable isotope labeling with amino acids in cell culture (SILAC) determined expression of proteins in the mitochondria. Results. 4-HNE impaired axonal regeneration, mitochondrial activity and induced aberrant axonal structures along the axons, which mimicked axon pathology observed in nerve isolated from diabetic rats and replicated aspects of neurodegeneration observed in human diabetic neuropathy. Proteins associated with mitochondrial dysfunction, oxidative phosphorylation and biosynthesis were down regulated in diabetic samples. The axons of diabetic neurons exhibited oxidative stress and depolarized mitochondria. CNTF and resveratrol reversed abnormalities in the mitochondrial membrane potential induced by diabetes and treatment of neurons with 4-HNE. CONCLUSIONS. Elevation of 4-HNE levels in diabetes was associated with impaired mitochondrial function and might be an important link between increased ROS levels and nerve degeneration in diabetic neuropathy. Abnormal mitochondrial function correlated with a down-regulation of mitochondrial proteins, with components of the respiratory chain targeted in lumbar DRG in diabetes. The reduced activity of the respiratory chain was associated with diminished superoxide generation within the mitochondrial matrix and did not contribute to oxidative stress in axons of diabetic neurons.
10

Τα ανθρώπινα πολυμορφοπύρηνα παράγουν Η2Ο2 το οποίο δρα ως σηματοδοτικό μόριο, κατά την κυτταροφαγία E. coli

Καραμολέγκου, Γεωργία 24 October 2012 (has links)
Η κυτταροφαγία βακτηρίων από τα πολυμορφοπύρηνα κύτταρα του αίματος του ανθρώπου επιτυγχάνεται με την ενεργοποίηση διαφόρων σηματοδοτικών μονοπατιών. Η σηματοδότηση ξεκινάει με την προσκόλληση των βακτηρίων στην πλασματική μεμβράνη των κυττάρων και καταλήγει στην αναδιοργάνωση του κυτταροσκελετού, στο σημείο επαφής, για τη δημιουργία του φαγοσώματος. Διάφορες μορφές δραστικού οξυγόνου, όπως το Η2Ο2, παράγονται παρουσία βακτηρίων, και δρουν ως σηματοδοτικά μόρια. Στην εργασία αυτή μελετήθηκε η παραγωγή και η συμμετοχή του Η2Ο2 στην κυτταροφαγία E.coli από τα ανθρώπινα πολυμορφοπύρηνα κύτταρα. Για το σκοπό αυτό, χρησιμοποιήθηκε το εξειδικευμένο φθορίζον μόριο, διυδροροδαμίνη (DHR) - που οξειδώνεται από το Η2Ο2 - καθώς και φθορίζοντα βακτήρια E. coli. Πειράματα κυτταρομετρίας ροής έδειξαν ότι τα πολυμορφοπύρηνα παράγουν Η2Ο2 παρουσία E. coli, ενώ παράλληλα δείχτηκε με έμμεσο τρόπο, με ανοσοαποτύπωση, η μετατόπιση της κυτταροπλασματικής υπομονάδας p47 για την συγκρότηση μεμβρανικής NADPH οξειδάσης. Το ένζυμο αυτό παράγει υπεροξεικά ιόντα τα οποία μετατρέπονται, στη συνέχεια, σε Η2Ο2 από τη δεσμουτάση SOD. Ο ενεργός ρόλος του Η2Ο2 στη ρύθμιση της κυτταροφαγίας, επιβεβαιώθηκε με τη χρήση εξειδικευμένων αναστολέων για τα ένζυμα της σύνθεσής του, N-εθυλμαλειμίδιο (N-ethylmaleimide - nem) για τη NADPH οξειδάση και διεθυλδιθειοκαρβαμικό (diethyldithiocarbamate – ddc) για την δεσμουτάση του υπεροξεικού ανιόντος. Οι αναστολείς αυτοί μείωσαν και την παραγωγή του Η2Ο2 και την κυτταροφαγία βακτηρίων σε καλλιέργεια λευκών αιμοσφαιρίων. Ανοσοαποτυπώματα για τις ΜΑΡ κινάσες, p38 και ΕΡΚ1/2, από εκχύλισμα λευκών αιμοσφαιρίων, μετά από καλλιέργεια παρουσία των παραπάνω αναστολέων, έδειξαν ότι κατά την κυτταροφαγία E. coli, μόνο η φωσφορυλίωση της ERK1/2 μειώνεται όταν μειώνεται η παραγωγή Η2Ο2. Είναι γνωστό ότι το Η2Ο2 απενεργοποιεί φωσφατάσες πρωτεϊνικών τυροσινών (PTPs). Από τα αποτελέσματα μπορεί να υποτεθεί ότι το Η2Ο2 που παράγεται κατά την κυτταροφαγία, απενεργοποιεί τις PTPs, ενισχύοντας τη δράση κινασών τη φωσφορυλίωση της ERK1/2 και να ολοκληρωθεί η διαδικασία της κυτταροφαγίας. Αναστολή της παραγωγής του Η2Ο2 αυξάνει τη δράση των PTPs, την αποφωσφορυλίωση της pERK1/2 και τελικά να μειώνει την κυταροφαγία. / Phagocytosis by human polymorphonuclear blood cells, is achieved by the activation of several signaling pathways. Signaling is initiated with the attachment of the bacteria on the plasma membrane of the cells and is completed with the remodeling of actin on that sight leading to the formation of the phagosome. Certain reactive oxygen species, such as Η2Ο2, which are produced in the presence of bacteria, have been referred to act as signaling molecules. In the present work, we investigated the production and participation of Η2Ο2 in the phagocytosis of E. coli by the human polymorphonuclear cells. For this purpose specific fluorescent probe dihydrorhodamine (DHR) - which is oxidised by H2O2 - and fluorescent E. coli were used. Flow cytometry analysis revealed the production of Η2Ο2 by polymorphonuclears, during phagocytosis of E. coli, while in parallel, it was indirectly shown with immunoblotting, the translocation of cytoplasmic p47 subunit for the assembly of the membrane NADPH oxidase. This enzyme produces superoxide ions which are then converted in H2O2 by superoxide dismutase (SOD). The active role of Η2Ο2, in the regulation of phagocytosis, was confirmed with the use of specific enzyme inhibitors of its synthesis, N-ethylmaleimide (nem) for the NADPH oxidase and diethyldithiocarbamate (ddc) for superoxide dismutase. These inhibitors decreased E. coli phagocytosis in polymorphonuclear cells cultures along with the decrease of Η2Ο2 production. Immunoblot analysis of ΜΑΡ kinases p38 and ERK1/2, from crude extracts of cultured white blood cells, in the presence of the above inhibitors, showed that the phosphorylation of ERK1/2 was reduced when H2O2 was blocked. On the other hand, p38 phosphorylation was not influenced at all. It is known that the target molecules of Η2Ο2 are a family of protein tyrosin phosphatases (PTPs) which are getting inactivate. From the above data, it may be assumed that Η2Ο2, which is produced by the polymorphonuclear blood cells, during phagocytosis, inactivates these enzymes, in order to promote the activity of kinases enhancing the phosphorylation of ERK1/2, among others, so that phagocytosis to be completed. Thus, when the H2O2 production is inhibited, the activity of the PTPs increases, leading to the dephosphorylation of pERK1/2 and the observed decrease of bacterial uptake.

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