No description available.

Background: Hypertension is one of the most common diseases in the United Kingdom and it remains an important risk factor for cardiovascular morbidity and mortality. Dietary sodium is an important trigger for hypertension and humans show a heterogeneous blood pressure (BP) response to salt intake. The mechanisms for this have not been fully explained, with renal sodium handling thought to play a central role. Animal studies have shown that dietary salt loading results in Na+ accumulation and lymphangiogenesis in skin mediated by vascular endothelial growth factor-C (VEGF-C), both attenuating the rise in BP. This represents an additional system for maintaining BP and volume homeostasis in response to salt load. The focus of this thesis is to determine whether these dermal mechanisms exist in humans. Methods: The technique of measuring skin Na+ and K+ using inductively coupled plasma optical emission spectrometry was developed in a pilot study of healthy adults. In a further study in healthy adults, the effects of dietary salt modulation on skin Na+, the effect of sex and the relationship between skin Na+ and haemodynamic parameters and plasma VEGF-C were studied. Skin Na+ concentrations were expressed as the ratio Na+:K+ to correct for variability in sample hydration. The effect of dietary salt intake on skin gene expression of factors that potentially influence BP such as VEGF-C and the hypoxia inducible factor (HIF) transcription system was assessed, exploring possible mechanisms linking skin Na+ to haemodynamic variables. Results: Skin Na+:K+ increased with dietary salt loading and this effect appeared to be greater in men while only women showed a rise in ambulatory mean BP. Skin Na+:K+ correlated with blood pressure, stroke volume and peripheral vascular resistance in men, but not in women. No change was noted in plasma vascular endothelial growth factor-C. Conclusions: These findings suggest that the skin may buffer dietary Na+, reducing the hemodynamic consequences of increased salt and this may be influenced by sex. Skin Na+ may influence blood pressure, stroke volume and PVR.

Agricultural production in Monterey County, California is a multi-billion dollar industry. Near the coast, seawater intrusion has threatened to degrade the groundwater quality due to over-pumping of the aquifer. The Monterey Regional Water Pollution Control Agency (MRWPCA), in partnership with the Monterey County Water Resources Agency, has provided recycled water since 1998 to over 12,000 acres of prime agricultural farmland in the northern Salinas Valley in an effort to reduce groundwater removal. The dominant soil types in the region are clay loam and clay soils, which are both susceptible to sodium (Na) accumulation and water infiltration problems. Recycled water blended with well water is used to irrigate cool season vegetables (i.e., artichokes, broccoli, Brussels sprouts, celery, cauliflower, and lettuce) and strawberries. A long-term study was implemented by MRWPCA to monitor salinity levels in commercial vegetable fields because of grower concerns that salts in the recycled water would have long term effects on soil quality. Accumulation of salts over time would make the soil less productive. Soil salinity levels were monitored at three Control and three Test Sites beginning in the spring of 2000. The Control Sites received well water, and the adjacent Test Sites received an approximate 2:1 blend of recycled and well water, respectively. Control and Test Sites were paired based on location to compare the same soil, crop, drainage systems, and farming practices. The soil was sampled three times per year from all sites: spring (before planting), mid-summer after harvest of the first crop, and late fall after the second crop harvest. Composites of four cores were collected at each site from the zero to 36-inch depth at 12-inch intervals. Each 12-inch interval soil sample was analyzed for pH, electrical conductivity (ECe), extractable cations (Na+, Ca2+, Mg2+, and K+) and extractable anions (Cl-, NO3-, and SO4-). After 10 years of monitoring, the data showed that using recycled water blended with well water at the Test Sites increased the ECe of the soil profile from 2.1 to 2.5 dS/m and increased the sodium adsorption ratio (SAR) from 3.0 to 3.9. The data also showed that using well water at the Control Sites increased the ECe of the soil profile from 1.4 to 2.6 dS/m and the change in SAR was negligible. The Test and Control Sites were significantly different for ECe and SAR, which was expected considering a higher salt content in the recycled water compared to the well water. The significant differences for ECe and SAR were associated with the significant differences in soil Na+ levels between the Test and Control Sites. The SAR and ECe of soil samples from all sites were in a range acceptable for vegetable production. The use of recycled water for irrigation of cool season vegetables and strawberries in the study area has not shown an indication of degraded soil productivity. Based on vegetable production and the slow increase of salts in the soil, recycled water can be used for long-term irrigation with proper management.

Soil salinity is a major concern to agriculture in arid and semiarid regions, where evapotranspiration causes salts originating from irrigation water (or sometimes naturally from the soil) to become concentrated in the rooting zone. In some areas, with good management, it has been economically feasible to ameliorate a sodic soil with Ca. The objective of this study was to investigate the effects of Ca amelioration of salinity (sodicity) on biomass, number of nodules, number of pods, weight of pods, ion uptake, and photosynthesis of Phaseolus vulgaris L. Pl ants were grown in one liter styrofoam pots under greenhouse conditions. In the first experiment, Na stress was accomplished by adding NaCl and Na2S04 at concentrations of 0, 20, 40, 60 , and 80 mmolcfl. The second and third experiments used concentrations of 0, 15, 30, 45, and 60 mmolc/1 NaCl or Na2S04 , combined with two levels of 15 and 30 rnrnolc/1 of either caso4 or cac12. Each styrofoam pot was irrigated with 300 ml of salt solution with a 0.25 leaching fraction on every fourth day for four weeks. Increasing Na concentration decreased biomass, number of nodules, number of pods, and weight of pods but increased ion uptake. Addition of NaCl in the substrate increased shoot Na, Ca, and Cl content, while Na2SO4 increased shoot S content. The photosynthesis rate was affected by all levels and types of sodium salts. Calcium sulfate treatments had a greater ameliorating effect than CaCL2 on Na induced salinity in snapbeans.

Glukosetransporter spielen eine wichtige Rolle in der Versorgung des Gehirns mit Nährstoffen und somit für den Erhalt der physiologischen Zellintegrität. Glukose wird über die Blut-Hirn-Schranke (BHS) mittels spezifischen transmembranen Transportproteinen der SLC-Genfamilie (GLUT, SGLT) befördert. Dabei scheint während physiologischen Bedingungen hauptsächlich der Glukosetransporter GLUT1 (SLC2A1) für die Energieversorgung des Gehirns zuständig zu sein. Die Erforschung der SGLT-Expression ist in den letzten Jahren ein wichtiger Ansatzpunkt für neue Behandlungsstrategien vieler Erkrankungen, wie Diabetes Mellitus, maligne Neoplasien oder eines Herzinfarkts, geworden. Jedoch ist über deren Expression und Funktion im menschlichen Gehirn nur wenig bekannt. Besonders die Lokalisation entlang der BHS bleibt fraglich. Ein Großteil bisheriger Forschungsarbeiten beschäftigt sich hauptsächlich mit der Expressionsanalyse des Transporters SGLT1 im tierischen Gehirn in vivo (Poppe et al. 1997; Balen et al. 2008; Yu et al. 2013). Es konnte aufgezeigt werden, dass SGLT1 mRNA exklusiv in Neuronen und nicht an der BHS exprimiert wird. Dies wird durch in vitro Analysen einer humanen Hirnendothelzelllinie bestätigt. Demnach kann kein SGLT1 unter physiologischen Bedingungen nachgewiesen werden (Sajja et al. 2014). Im menschlichen Hirngewebe besitzen SGLTs somit keine zentrale Funktion für den Glukosetransport an der BHS. Im Gegensatz dazu konnte eine Expression von SGLT sowohl in vivo als auch in vitro während hypoglykämischen Bedingungen belegt werden (Vemula et al. 2009; Sajja et al. 2014). Die Expression der SGLT-Transporter während einer ischämischen Hypoglykämie führt zu der Annahme, dass diese Transporter für die Aufrechterhaltung der Energieversorgung des geschädigten Hirngewebes notwendig sind. Um die physiologischen Mechanismen nach einem Glukosemangel zu untersuchen, wurden SHT-Modelle etabliert (Salvador et al. 2013). In einem experimentellen Modell des Schädel-Hirn-Traumas im Rahmen eines DFG-gefördertes Projekts ist ein Expressionsverlauf von Glukosetransportern im Maushirn und in Hirnendothelzellen erarbeitet worden (Wais 2012; Salvador et al. 2015). Somit könnten SGLTs als Ansatzpunkt für den Nachweis der Überlebenszeit nach einem SHT fungieren. Die vorliegende Arbeit fokussiert sich auf die Expression der Natrium-abhängigen Glukosetransporter SGLT1 und SGLT2 im menschlichen Gehirn. Hierbei liegt das Hauptaugenmerk auf der Lokalisation dieser Transporter an der menschlichen BHS von post mortalem Hirngewebe. Weiterhin wird untersucht ob die Expressionsstärke von SGLT1 und SGLT2 eine Aussage über die Überlebenszeit von Verstorbenen nach einer traumatisch bedingten Hirnveränderung zulässt. Die Lokalisation von SGLT1 und SGLT2 an der menschlichen BHS konnte durch die Etablierung eines Protokolls zur Isolation von Hirnkapillaren erfolgen. Vorab wurden alle verwendeten Antikörper auf ihre Spezifität mittels siRNA Transfektion und Blockierung der Immunfluoreszenzsignale mittels immunisierten Peptids getestet. Somit ist die Spezifität der detektierten SGLT1- und SGLT2-Expression in menschlichen Hirnkapillaren gewährleistet. Anschließend wird untersucht, in welchen zeitlichem Verlauf nach einer traumatisch bedingten Hirnveränderung die verschiedenen Formen der Glukosetransporter exprimiert werden und ob ggf. der Umfang und die Verteilung von SGLT1, SGLT2 und GLUT1 sowie das Verhältnis zueinander Auskünfte über eine vitale bzw. postmortale Entstehung eines Traumas bzw. dessen Überlebenszeit zulässt. Hierfür wird ein Expressionsschema der Glukosetransporter generiert, abhängig von Todeszeitpunkt und Todesursache. Es konnte festgestellt werden, dass GLUT1 nicht als Target für die Ermittlung der Überlebenszeit nach einem Trauma geeignet ist. Dahingegen zeigen SGLT1 und SGLT2 eine signifikante Änderung der Expressionsstärke im contusionalen Gewebe in Abhängigkeit von der Überlebenszeit. Obwohl diese vorläufigen Daten einen neuen Ansatzpunkt für die forensische Fragestellung aufzeigen, müssen weitere Experimente mit einem erhöhten Umfang der Probenanzahl und kürzere Zeitspannen der Überlebenszeiträume durchgeführt werden. / The transport of glucose across the endothelial cells of the human blood-brain barrier (hBBB) plays a major role for energy supply of the brain and therefor for neuronal integrity. Glucose enters the brain cells through specific transmembrane transporter proteins of the SLC-gene family (GLUT, SGLT). Under physiological conditions glucose uptake across the BBB seems to be mediated primarily by facilitated diffusion through glucose transporter 1 (GLUT1). Although SGLTs are a known drug target for diabetes and furthermore play a role in other disease like cancer and cardiac ischemia, active glucose transport by SGLTs is hardly observed and very little is known about their expression or activity in human brain. Especially the function along the BBB remains uncertain. Up to now, expression analysis focused on SGLT1 and has been confirmed in vivo by analyzing brain tissue of animals (Poppe et al. 1997; Balen et al. 2008; Yu et al. 2013). Here detection mainly occurs in neurons, no SGLT1 mRNA in capillaries of the BBB could be found. Similarly in vitro experiments with a human brain microvascular endothelial cell line reveals no expression of SGLT1 under physiological conditions (Sajja et al. 2014). In human brain, SGLT1 is hardly expressed and so far could not be found along the BBB. In contrast to these findings, expression of SGLT1 could be detected in vivo as well as in vitro under hypoglycemic conditions (Vemula et al. 2009; Sajja et al. 2014). The occurrence of these transporters during ischemic hypoglycemia could lead to the conclusion that the secondary active glucose transport by SGLTs is necessary for additional glucose supply in injured brain. To investigate if SGLTs are required for the reconstruction of energy supply after glucose deficiency, traumatic brain injury (TBI) models were established to study secondary physiological mechanisms along the BBB (Salvador et al. 2013). In an experimental CCI (controlled cortical impact) mouse model within a DFG-funded project, an expression pattern of glucose transporters in the mouse brain and in brain endothelial cells has been developed (Wais 2012; Salvador et al. 2015). Thus it could lead as a Target for evidence of the time of survival after TBI. This study focuses on the sodium-dependent glucose transporters SGLT1 and SGLT2 expression in human brain. The main topic is to localize the sodium-dependent glucose transporters along the human BBB of post mortem brain tissue and to examine whether SGLT expression allow a conclusion to be drawn about the survival time of a patient after TBI. First of all the localization of SGLT1 and SGLT2 at the human BBB could be shown by establishment a capillary isolation protocol of human post mortem brain tissue. Therefore the antibody specificity was tested by a siRNA transfection protocol and blocking the immunofluorescence signal with an immunized peptide. Thus, specific SGLT1 and SGLT2 expression at the endothelial lining of the capillary lumen could be demonstrated. After attaching the value of SGLTs at the human BBB, the relationship of the glucose transporter expression in TBI tissue according to the survival time of the patient is presented. Hereby it should be clarified whether the expression and distribution of the transporters GLUT1, SGLT1 and SGLT2 as well as the relation to each other provide information on a vital or post mortal development of a trauma or its survival time. It could determine that GLUT1 is not suitable as a target for the representation of survival time after TBI. However, SGLT1 and SGLT2 show a significant change in the expression profile of traumatic brain regions. Here an increase according to the survival time after trauma can be shown. Although these preliminary data suggest a novel target for forensic questions, more experiments with an increased scope of survival time frames should be carried out.

No description available.

This study reports results obtained and the approach taken in investigating the temporal sodium flux in a woodlot soil receiving secondary treated effluent at Branxton, NSW. Previous research has shown woodlot soils receiving secondary treated effluent undergo an increase in exchangeable sodium percentage (ESP) over time. Increased soil ESP influences micro-aggregate/soil pore stability and, particularly when subject to irrigation waters of specific low-electrolyte concentrations, results in decreased soil permeability and a subsequent need to reduce effluent application rates. Therefore, in irrigated woodlot soils it has been necessary to implement strategies to remove excess sodium from the root zone to maintain optimum permeability of the receiving soil, that is, maintaining the cation balance (as soil ESP) to promote optimum soil pore size. To maintain optimum permeability, an understanding is needed of temporal variations in the accumulation/leaching (flux) of sodium within a soil under secondary treated effluent irrigated conditions. The ability to define the sodium flux depends on the frequency of soil sampling and the ability to interpret the net loss/gain in soil sodium in relation to the applied hydraulic load over time. Past research has measured changes in soil ESP on an annual basis, or longer, making it impossible to interpret temporal sodium flux within a given year. The rate of change of soil ESP has ramifications for optimum permeability within an effluent irrigated woodlot. With respect to increasing/decreasing soil ESP, a major response of the clay particles within micro-aggregates is the deformation of conducting soil pores and reduced hydraulic conductivities. In addition, clay dispersion is governed by the soil ESP and electrolyte concentration of the infiltrating waters at the time, where dispersed clay particles may block conducting soil pores and further reduce hydraulic conductivity. Therefore, investigating the temporal sodium flux in conjunction with the temporal variation in electrolyte concentration of infiltrating waters will give greater insight into the response of effluent irrigated soils to sodium-rich waters over time. Three research aims were formed to investigate temporal sodium flux. These include: 1. To investigate trends in the dominant water balance components for a woodlot soil receiving secondary treated effluent (STE); 2. To examine temporal and spatial variation in both the water balance components and measured soil properties, particularly the sodium flux; and 3. To investigate the implications of the sodium flux on the loss of soil structure and drainage over time (dispersion events), particularly in relation to temporal changes in soil ESP and effluent SAR. Monitoring programs for water balance components and soil parameters covered the period January 2002 – October 2003. Every two months, soil samples were taken at designated sites and at different depths (10, 20, 40, 60, and 80 cm). These samples were analysed for exchangeable cations (Ca2+, Mg2+, Na+ and K+), from which the ESP values were derived. Also, this appears to be the first time that soil sampling at this frequency, which enables the temporal sodium flux to be determined, has been carried out. Column leaching experiments were also performed over the study period to illustrate the response of the woodlot soil, in terms of micro-aggregate stability, to hydraulic loads of varying SAR. Column leaching experiments also confirmed the rate of solute movement through the soil profile and the woodlot soil’s ability to bind/exchange sodium under different hydraulic loads and electrolyte concentrations. Soil extraction plate methods were used to determine wilting point and field capacity for these soils. The Sodium Adsorption Ratio (SAR), which is the solutional equivalent to soil ESP, was used to define the electrolyte concentration of the applied effluent and rainfall to the woodlot. The net loss/gain of exchangeable sodium at different depths and times was determined and compared with changes in water balance components and the measured volumetric soil water over time. The soil water surplus/deficit was recorded at a daily time-step and a cumulative approach was used to determine the long-term soil water surplus/deficit. In addition, variations in groundwater levels were monitored to observe if surplus irrigation events were reflected in temporal trends. As a result of determining the temporal variation in soil ESP, effluent and rainfall SAR, daily soil water deficit/surplus (short-term), cumulative soil water deficit/surplus (long-term) and volumetric soil moisture, temporal trends are presented. The sodium flux was then investigated by interpreting trends in the monitored data with respect to the dominant water balance components. All parameters were then used to model the potential dispersive behaviour of the receiving soil over time and depth, in relation to the volume and electrolyte concentration of the effluent and rainfall applied over time. The implications for soil structure and permeability depend on variations in soil ESP and effluent SAR. Results from this research show that soil ESP varied by as much as 24% over a four-month period and is shown to be a function of the sodium loading (from STE) and soil water surplus/deficit. On each sampling occasion, soil ESP generally increased with depth at all irrigated sites. Soil ESP at non-irrigated sites was much lower than irrigated sites, although the variability in soil ESP was much greater. Variations in SAR of the waters received by the woodlot soil (effluent and rainfall) over the study period ranged from 0.5 to 5.9. It is shown that the SAR range, coupled with variations in soil ESP, has ramifications for maintaining long-term soil structure. Soil structure at different sites within a woodlot will respond differently according to the soil ESP/effluent SAR relationship. The dispersive potential of soil at a given ESP receiving irrigation waters of known SAR was assessed in light of the relationship between soil ESP and effluent SAR. This showed the dynamic response of effluent irrigated soils to the long-term temporal variation in electrolyte concentration of rainfall/effluent. The relationship between soil ESP and effluent SAR is graphically presented as a continuum, which in turn can be used as a management tool for assessing the potential for dispersion of clay particles in a soil of known ESP and irrigated with waters of known SAR. By identifying trends in the temporal sodium flux, the optimum permeability of the receiving soil can be assessed in relation to the electrolyte concentration of the applied waters and the soil exchangeable sodium percentage (ESP). Secondary treated effluent application rates can then be corrected to prevent “dispersive” irrigation events over the long term and/or management strategies applied to remove excess sodium from the soil profile. The significance of the research is that a better understanding of the temporal dynamics of sodium in the soil profile will allow improved management of effluent irrigated woodlots, with the aim of making the practice sustainable with respect to controlling accumulating soil sodium and maintaining soil structure for future landuse. / PhD Doctorate

The epithelial sodium channel (ENaC) is the central component of the sodium absorption pathway in epithelia. It is critical for sodium homeostasis and blood pressure control, which is demonstrated by rare genetic disorders such as Liddle�s syndrome and pseudohypoaldosteronism type I, that are associated with hyper- and hypotension, respectively. ENaC is mainly regulated by mechanisms that control the expression of active channels at the cell surface. Ubiquitin ligases of the Nedd4-like family, such as Nedd4 and Nedd4-2 decrease epithelial sodium absorption by binding to and targeting ENaC for endocytosis and degradation. This is most likely achieved by catalyzing the ubiquitination of ENaC. Conversely the serum- and glucocorticoid regulated kinase (SGK) increases ENaC activity. This effect is partly mediated by the interaction of SGK with the ubiquitin ligases Nedd4 and Nedd4-2. SGK is able to bind to both Nedd4 and Nedd4-2, however only Nedd4-2 is phosphorylated by SGK. The phosphorylation of Nedd4-2 inhibits its interaction with ENaC, thus reducing ENaC ubiquitination, thereby increasing surface expression and sodium absorption. Nedd4-like proteins interact with ENaC via their WW-domains. These domains bind PY-motifs (PPXY) present in ENaC subunits. Nedd4 and Nedd4-2 both have four highly similar WW-domains. Previous studies have shown that interaction between Nedd4 and ENaC is mainly mediated by WW-domain 3. SGK also has a PY-motif; therefore it was analyzed whether the WW-domains of Nedd4 and Nedd4-2 mediate binding to SGK. Here, it is shown that single or tandem WW-domains of Nedd4 and Nedd4-2 mediate binding to SGK and that, despite their high similarity, different WW-domains of Nedd4 and Nedd4-2 are involved. These data also suggest that WW-domains 2 and 3 of Nedd4-2 mediate the interaction with SGK in a concerted manner, and that in vitro the phosphorylation of SGK at serine residue 422 increases its affinity for the WW-domains of Nedd4-2. The stimulatory effect of SGK on ENaC activity is partly mediated via Nedd4-2 and will decrease if competition between Nedd4 and Nedd4-2 for binding to SGK occurs. Here it is shown that Nedd4 and Nedd4-2 are located in the same subcellular compartment and that they compete for binding to SGK. Besides its function in the proteasomal degradation pathway ubiquitination is involved in the regulation of membrane protein trafficking, including their endocytosis. ENaC was shown previously to be ubiquitinated. Here, we provide evidence that ENaC can be ubiquitinated differentially depending on its cellular location. Channels residing in the plasma membrane are multiubiquitinated and we suggest that this serves as an internalization signal for ENaC and a control for further trafficking. Cytosolic ENaC is mainly polyubiquitinated, and therefore probably targeted for proteasomal degradation. However, mono- and multiubiquitination of ENaC located within the cytosol is very likely to occur as well. In addition, it is shown that both proteasomal and lysosomal pathways are involved in the regulation of ENaC.

The metabolic heat generated by exercise must be dissipated to maintain body temperature within narrow physiological limits; during exercise and heat exposure, body water is lost via sweating to enable evaporative cooling of the body. When sweating takes place, total body water is reduced (without the intake of additional fluids) from each fluid compartment due to the free exchange of water between compartments with a concomitant loss of electrolytes, primarily sodium. A series of three investigations were undertaken to evaluate: 1) the efficacy of acute sodium citrate-chloride loading on endurance trained males and females as a viable means to expand extracellular fluid volume, 2) any menstrual cycle effects on renal handling of this sodium load at rest, and 3) if any subsequent hypervolaemia reduces the physiological strain of exercise in warm conditions in both genders. The first investigation examined eight endurance-trained (VO₂[max]: 58 ml�kg⁻��min⁻� (SD 5); 36 y (SD 11)) runners in a randomized double-blind crossover study. The participants ingested a high-sodium (HighNa⁺: 164 mmol Na⁺�L⁻�) or low-sodium (LowNa⁺: 10 mmol Na⁺�L⁻�) beverage (10 ml�kg⁻�) before running to exhaustion at 70% VO₂[max] in warm conditions (32�C, 50% RH, V[a]~1.5 m�s⁻�). Results indicate that HighNa⁺ increased PV before exercise (4.5% (SD 3.7)), calculated from Hct and [Hb]), whereas LowNa⁺ didn�t (0.0% (SD 0.5); P = 0.04), and involved greater time to exercise termination in those who were stopped due to ethical end point of 39.5�C and volitional exhaustion (39.5�C: 57.9 min (SD 6) vs. 46.4 min (SD 4); n = 5, P = 0.04; EXH: 96.1 min (SD 22) vs. 75.3 min (SD 21); n = 3, P = 0.03; HighNa⁺ vs. LowNa⁺ respectively). At equivalent times before exercise termination, HighNa⁺ also involved lower core temperature (38.9 vs. 39.3�C; P = 0.00) and perceived exertion (P = 0.01), and a tendency for lower heart rate (164 vs. 174 bpm; P = 0.08). The main purpose of the second investigation was to investigate the efficacy of an acute sodium load on endurance trained women�s plasma volume and renal mechanisms across the menstrual cycle at rest. This was evaluated by inducing a sodium-mediated plasma volume expansion using HighNa⁺ at rest during the last high hormone week of the OCP cycle (HH[ocp]) or the late-luteal phase of the natural cycle (LUT[nat]) and during the low hormone sugar pill week of the OCP cycle (SUG[ocp]) or during the early follicular phase of the natural cycle (FOL[nat]. Thirteen women completed the study with one woman on a progestin-only pill (results were used for case study, not statistical analyses) and were assigned to one of two groups: 1) control (NAT, n = 6, 24 y (SD 5), 53 ml�kg�ml⁻� (SD 3)) or oral contraceptive pill (OCP, n = 6, progestin only n = 1, 29 y (SD 6), 51 ml�kg�ml⁻� (SD 2)) group according to their usage status. Across the four-hour post loading time there was greater plasma volume expansion in SUG[ocp] and FOL[nat] vs. LUT[nat] and HH[ocp] (5.06% (SD1.16) vs. 3.35% (SD 0.23), P = 0.02). OCP usage did not reliably alter the hypervolaemic response (P = 0.27), and this was not dependent on phase of cycle (P = 0.32). Plasma volume expansion occurred across both types and phases of the menstrual cycle with evidence that estradiol interactions with AVP, P[osm] and body water retention are stronger in the low hormone phase of the OCP than in the follicular phase of the natural cycle; illustrated by greater overall water retention after an acute sodium+water load. The third investigation was conducted during the high hormone phase of both OCP and NAT menstrual cycles to further examine sodium-loading effects on the physiological capacity of exhaustive cycling in warm conditions. Thirteen endurance-trained (VO₂[peak] 52 ml�kg⁻��min⁻� (SD 2); 26 y (SD 6), 60.8 kg (SD 5), mean (SD)) cyclists completed this double-blind, crossover experiment during the high hormone phase of the menstrual cycle. Cyclists ingested a concentrated sodium (HighNa⁺: 164 mmol Na⁺�L⁻�) or low-sodium (LowNa⁺: 10 mmol Na⁺�L⁻�) beverage (10 ml�kg⁻�) before cycling to exhaustion at 70% VO₂[max] in warm conditions (32�C, 50% RH, V[a]~5.6 m�s⁻�). HighNa⁺ increased PV before exercise, similar to that of the men in the first investigation, whereas LowNa⁺ didn�t (4.4% (SD 1.2) vs. -1.9% (SD 1.3); P < 0.0001), and involved greater time to exhaustion (98.6 min (SD 25.6) vs. 78.5 min (SD 24.6); P < 0.0001). There was a higher baseline core temperature and faster rate of change for HH[ocp] for both beverage conditions (HighNa⁺: 37.15 (SD 0.6) vs. 36.92�C (SD 0.4); P = 0.05, LowNa⁺: 37.04 (SD 0.6) vs. 36.90�C (SD 0.4), P = 0.05; HH[ocp] vs. LUT[nat], respectively). Through this series of investigations a greater understanding was achieved of fluid balance and the effect of pre-exercise hypervolaemia between genders; pre-exercise ingestion of a concentrated sodium beverage increased plasma volume before exercise and involved less thermoregulatory and the actual and perceived physiological strain during exercise and increased endurance in warm conditions.

Includes bibliographical references. / 1 v. in various pagings : / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / Thesis (D.Sc.)--University of Adelaide, Dept. of Botany, 1994