No description available.

This thesis identifies, describes and assesses the leading features of Evangelicalism as exhibited in the writings and episcopate of John Charles Ryle, first Bishop of Liverpool, 1880-1900. Chapter One attempts a synthesis of his theology through his extant sermons. Chapter Two describes his understanding of the dangers facing the Church of England oh account of the Disestablishment of the Irish Church and the presence of 'Romanism' within the Church. Chapter Three outlines Ryle's strategy of evangelism in the city of Liverpool, while Chapter Four identifies his involvement in contemporary social issues. Chapters Five and Six look at ways in which this mission activity was hindered and obstructed by other clergy, by other diocesan activity, by financial constraints and by the problem of Ritualism, especially the prosecution of James Bell Cox. As a result of this study it is possible to identify changes in both Evangelical theology and practice in the last decades of the nineteenth century.

The Cuntz semigroup is an isomorphism invariant for C*-algebras consisting of a semigroup with a compatible (though not algebraic) ordering. Its construction is similar to that of the Murray-von Neumann semigroup (from which the ordered K_0-group arises by the Grothendieck construction), but using positive elements in place of projections. Both rich in structure and sensitive to subtleties of the C*-algebra, the Cuntz semigroup promises to be a useful tool in the classification program for nuclear C*-algebras. It has already delivered on this promise, particularly in the study of regularity properties and the classification of nonsimple C*-algebras. The first part of this thesis introduces the Cuntz semigroup, highlights structural properties, and outlines some applications. The main result of this thesis, however, contributes to the understanding of what the Cuntz semigroup looks like for particular examples of (nonsimple) C*-algebras. We consider separable C*-algebras given as the tensor product of a commutative C*-algebra C_0(X) with a simple, approximately subhomogeneous algebra A, under the regularity hypothesis that A is Z-stable. (The Z-stability hypothesis is needed even to describe of the Cuntz semigroup of A.) For these algebras, the Cuntz semigroup is described in terms of the Cuntz semigroup of A and the Murray-von Neumann semigroups of C(K,A) for compact subsets K of X. This result is a marginal improvement over one proven by the author in [Tikuisis, A. "The Cuntz semigroup of continuous functions into certain simple C*-algebras." Internat. J. Math., to appear] (there, A is assumed to be unital), although improvements have been made to the techniques used. The second part of this thesis provides the basic theory of approximately subhomogeneous algebras, including the important computational concept of recursive subhomogeneous algebras. Theory to handle nonunital approximately subhomogeneous algebras is novel here. In the third part of this thesis lies the main result. The Cuntz semigroup computation is achieved by defining a Cuntz-equivalence invariant I(.) on the positive elements of the C*-algebra, picking out certain data from a positive element which obviously contribute to determining its Cuntz class. The proof of the main result has two parts: showing that the invariant I(.) is (order-)complete, and describing its range.

The Cuntz semigroup is an isomorphism invariant for C*-algebras consisting of a semigroup with a compatible (though not algebraic) ordering. Its construction is similar to that of the Murray-von Neumann semigroup (from which the ordered K_0-group arises by the Grothendieck construction), but using positive elements in place of projections. Both rich in structure and sensitive to subtleties of the C*-algebra, the Cuntz semigroup promises to be a useful tool in the classification program for nuclear C*-algebras. It has already delivered on this promise, particularly in the study of regularity properties and the classification of nonsimple C*-algebras. The first part of this thesis introduces the Cuntz semigroup, highlights structural properties, and outlines some applications. The main result of this thesis, however, contributes to the understanding of what the Cuntz semigroup looks like for particular examples of (nonsimple) C*-algebras. We consider separable C*-algebras given as the tensor product of a commutative C*-algebra C_0(X) with a simple, approximately subhomogeneous algebra A, under the regularity hypothesis that A is Z-stable. (The Z-stability hypothesis is needed even to describe of the Cuntz semigroup of A.) For these algebras, the Cuntz semigroup is described in terms of the Cuntz semigroup of A and the Murray-von Neumann semigroups of C(K,A) for compact subsets K of X. This result is a marginal improvement over one proven by the author in [Tikuisis, A. "The Cuntz semigroup of continuous functions into certain simple C*-algebras." Internat. J. Math., to appear] (there, A is assumed to be unital), although improvements have been made to the techniques used. The second part of this thesis provides the basic theory of approximately subhomogeneous algebras, including the important computational concept of recursive subhomogeneous algebras. Theory to handle nonunital approximately subhomogeneous algebras is novel here. In the third part of this thesis lies the main result. The Cuntz semigroup computation is achieved by defining a Cuntz-equivalence invariant I(.) on the positive elements of the C*-algebra, picking out certain data from a positive element which obviously contribute to determining its Cuntz class. The proof of the main result has two parts: showing that the invariant I(.) is (order-)complete, and describing its range.

The hepatitis C virus (HCV) currently infects 3% of the world???s population, with chronic infection in 50-80% of exposed individuals. A small subset of individuals who are exposed to HCV do not develop anti-HCV antibodies, persistent viraemia or chronic hepatitis despite generating HCV-specific CD4+ and CD8+ T cells. These individuals are believed to develop an immune response which rapidly clears viraemia prior to the induction of an antibody response. Circumstantial evidence supports the likelihood that some of these individuals may generate these same responses and outcomes on repeated occasions of HCV infection. HCV-specific cellular immune responses in seronegative subjects have been the subject of only limited prior study, in part due to the lack of appropriate recombinant antigens and assay systems. Therefore, this thesis described the development and validation of an interferon-? (IFN-?) ELISPOT assay using overlapping peptides (n=441). Using this assay, HCV-specific cellular immune responses were detected in 5/10 (50%) of chronically infected subjects. Responses were identified more frequently, and were directed against more regions of the HCV genome, than with traditional assay systems. This IFN-? ELISPOT assay, a comparable interleukin (IL)-2 ELISPOT assay, and a multiplex in vitro cytokine production assay were then used to evaluate HCV-specific cellular immune responses in three cohorts of seronegative subjects at high-risk of exposure to HCV ??? babies born to infected mothers, multiply-transfused subjects with thalassaemia, and high risk injecting drug users. Cellular immune responses were evaluated in 23 infants born to HCV-antibody positive women. Responses were not detected in infants born to HCV-PCR negative mothers. IFN-? production was detected in 1/11 infants born to viraemic mothers using the ELISPOT assay, with cytokine production observed in an additional 3/5 infants studied using the in vitro cytokine production assay. HCV-specific cellular immune responses were assessed in a cohort of multiply transfused subjects with thalassaemia using assays for cytotoxic T lymphocyte activity, IFN-? and IL-2 ELISPOT, as well as lymphocyte proliferation and in vitro cytokine production. Responses were detected in 6/13 chronically infected subjects (46%), 4/7 subjects who had cleared infection (71%), and 14/17 seronegative subjects (82%). The seronegative subjects had responses which were broader and higher in magnitude than those with chronic HCV infection, although lower and narrower than in subjects who had cleared prior HCV infection. IFN-? and IL-2 ELISPOT assays, in additional to in vitro cytokine production assays, were performed on 41 injecting drug users (IDUs), with responses detected in 6 (15%). Seronegative IDUs with HCV-specific cellular immune responses had been injecting for a mean of 7.7 years, and reported multiple risk factors for exposure to HCV. The combined data from these three cohorts indicate that the HCV-specific cellular immune responses detected in seronegative subjects were generally broad in specificity. Cytokine production was generally Th1-biased, a pattern which has previously been associated with an increased likelihood of clearance in primary infection. The findings also suggest that responses can be maintained for decades after exposure, and may provide protection against repeated exposures. In summary, cellular immunity against HCV is evident in some seronegative high risk subjects, suggesting that the cellular immune responses may efficiently facilitate viral clearance. Understanding the mechanisms of this immune response pattern will allow better understanding of the host response to HCV and may provide key insights into vaccine design.

This thesis reports the synthesis and copper coordination chemistry of covalently-linked aryl-imidazole derivatives designed as models for the crosslinked imidazole-phenol sidechains of the His-Tyr cofactor in the CcO. Three new imidazole- (HL1 - HL3) and three new indole- (HL4 - H2L6) containing tripodal ligands were synthesised. The conjugate addition of an imidazole to activated quinone derivatives was developed as a new route to organic models for the Tyr His cofactor. Two monodentate imidazole-aryl, Im-hq(OH)2 and Im-ArOH, and an imidazole-quinone, Im bq were obtained using this route. The X-ray crystal structure of Im-hq(OH)2.EtOH was determined. The route was also used to give new chelating ligands, H2L10 and HL12, containing a cross-linked imidazole-phenol surrogate for the Tyr244-His240 cofactor. Copper complexes of Im-hq(OH)2, Im-bq, Im-ArOH, H2L10-HL12, and HL1-H2L6 were prepared, and the X-ray crystal structures of [Cu(terpy)(Im-bq)][BF4]2 and five other copper complexes were determined. The physiochemical properties of the copper complexes were characterized by FT-IR, UV-Vis-NIR, EPR and (spectro)electrochemical studies. Key results include: the oxidation of Im-ArO- anion affords the semiquinone radical, Im-sq(4OH)(1O??????), in a hydrous solvent. However, the oxidations of neutral Im-ArOH and [Cu(tpa)(Im-ArOH)]2+ produce the corresponding phenoxy radical species that rapidly and reversibly dimerise to give quinol cyclohexadienone, QCHD, dimers. Significantly [Cu(tpa)(Im-sq(4OH)(1O??????))]2+ was EPR silent, perhaps due to antiferromagnetic coupling between the Cu(II) (S=1/2) and semiquinonyl radical (S=1/2) centres. Deprotonation of the hydroquinone in [Cu(tpa)(Im-hq(OH)2]2+ produces the hydroquinone dianion which reduces the Cu(II) centre. The semiquinone radical is coordinatively labile and dissociates from the Cu(I) centre. The biological implications of these results are mentioned.

Infection with hepatitis C virus (HCV) causes significant morbidity and mortality. An understanding of the factors associated with both acquisition and clearance of HCV infection is critical to prevention strategies including vaccine development. Although research in the prison environment is logistically challenging, inmates are a premier risk group. Accordingly, a prospective cohort study of prisoners with monthly sampling for HCV viraemia was undertaken to assess the incidence of, and risk factors for, infection; and to assess the natural history of infection when detected by viraemia. The incidence of infection was 8 per 100 person years, with the incidence of "high risk" and "possible" HCV transmission risk events being 61 and 210 per 100 person years respectively. The first case of HCV infection in prison with tattooing as the probable route of acquisition was reported. A novel phenotype of HCV infection with HCV viraemia and subsequent clearance without the development of symptoms, biochemical hepatitis or seroconversion on HCV specific enzyme immunoassay (EIA), despite more than one year of follow-up, was reported. HCV-specific cell mediated immune responses were detected in the subjects analysed. These subjects also had indeterminate HCV serological responses directed against non-structural proteins detected on a recombinant immunob10t assay (RIBA) that were stable over time and typically predated HCV viraemia. The prevalence of such responses ranged from 29-79% in other relevant cohorts, including injecting drug users (IDUs) and multiply-transfused patients with thalassaemia. The antibody response against the non-structural protein, NS5 was the most reproducible. This reactivity was blocked in 57% of subjects when sera were pre-incubated with recombinant HCV proteins, suggesting HCV-specificity. A case-control study was undertaken to examine whether such responses predicted protection from "classical" HCV infection with EIA seroconversion. Cases that developed HCV viraemia and EIA seroconversion were more likely to have these responses at baseline (when aviraemic) than controls, demonstrating that they do not protect against acute infection. However, the rate of viral clearance in subjects with indeterminate RIBA responses that subsequently developed acute infection and were followed for viral clearance was high (88%), suggesting that such subjects have immune responses that are associated with viral clearance.

Thesis (Ph. D.)--University of Adelaide, Dept. of Mathematical Physics,1986. / Erratum (14 leaves) in pocket. Includes bibliographical references (leaves 124-128).

Thesis (M.A.)--Wheaton College Graduate School, 2003. / Abstract. Includes bibliographical references.

Diss. (sammanfattning) Uppsala : Univ., 2004. / Härtill 4 uppsatser.