<p> The underlying goal of this dissertation is to understand how the amygdala, a brain region involved in establishing the emotional significance of sensory input, contributes to the processing of complex sounds. The general hypothesis is that communication calls of big brown bats (<i>Eptesicus fuscus</i>) transmit relevant information about social context that is reflected in the activity of amygdalar neurons. </p><p> The first specific aim analyzed social vocalizations emitted under a variety of behavioral contexts, and related vocalizations to an objective measure of internal physiological state by monitoring the heart rate of vocalizing bats. These experiments revealed a complex acoustic communication system among big brown bats in which acoustic cues and call structure signal the emotional state of a sender. </p><p> The second specific aim characterized the responsiveness of single neurons in the basolateral amygdala to a range of social syllables. Neurons typically respond to the majority of tested syllables, but effectively discriminate among vocalizations by varying the response duration. This novel coding strategy underscores the importance of persistent firing in the general functioning of the amygdala. </p><p> The third specific aim examined the influence of acoustic context by characterizing both the behavioral and neurophysiological responses to natural vocal sequences. Vocal sequences differentially modify the internal affective state of a listening bat, with lower aggression vocalizations evoking the greatest change in heart rate. Amygdalar neurons employ two different coding strategies: low background neurons respond selectively to very few stimuli, whereas high background neurons respond broadly to stimuli but demonstrate variation in response magnitude and timing. Neurons appear to discriminate the valence of stimuli, with aggression sequences evoking robust population-level responses across all sound levels. Further, vocal sequences show improved discrimination among stimuli compared to isolated syllables, and this improved discrimination is expressed in part by the timing of action potentials. </p><p> Taken together, these data support the hypothesis that big brown bat social vocalizations transmit relevant information about the social context that is encoded within the discharge pattern of amygdalar neurons ultimately responsible for coordinating appropriate social behaviors. I further propose that vocalization-evoked amygdalar activity will have significant impact on subsequent sensory processing and plasticity.</p>

<p>Estradiol modulates the use of spatial navigation strategies in female rats. The presence of circulating estradiol enhances learning on tasks that require the use of a hippocampus-dependent place strategy and impairs learning on tasks that require the use of a dorsal striatum-dependent response strategy. When either strategy may be used successfully, estradiol biases females to use a place strategy. While this behavioral effect has been well-described in the young adult female rat, little is known about the mechanisms in the brain that underlie it or how it changes across age. The experiments in this dissertation examined how age, previous experience, and hormonal condition affect the ability of estradiol to modulate learning during explicit training of place and response tasks, as well as navigation strategy use during ambiguous navigation tasks. Age highly influenced the ability of estradiol to influence strategy use. While female rats could use place and response strategies to navigate by postnatal day (PD) 21, estradiol did not bias them to use a response strategy until PD26, just before puberty. In adulthood, previous navigation experience and estradiol interacted to influence navigation strategy use on a series of experiences to an ambiguous navigation task. And, estradiol impaired learning during explicit response training but did not affect place learning. In middle age, estradiol further impaired response learning but still did not affect place learning. Long-term hormone deprivation, however, was detrimental to acquisition of a place task but did not affect response learning. These experiments also examined the effects of estradiol on activity, plasticity, and reliability of neuronal ensembles in several subregions of the hippocampus and striatum during spatial navigation using cellular and molecular techniques that take advantage of the kinetics of the immediate-early genes c-fos and Arc. Increased activation and plasticity during active exploration across several subregions of the hippocampus and striatum reflected similar inputs to these neural systems and similar effects of exploration. However, estradiol modulated the plasticity and reliability of neuronal ensembles in the hippocampus and striatum specifically during goal-directed spatial navigation. Estradiol increased plasticity in CA1 of all behaviorally-trained rats, but only place strategy users displayed high reliability in this plasticity across training and probe trials on a navigation task. Estradiol prevented increase in plasticity and reliability in the dorsolateral striatum displayed by low estradiol response strategy users. These experiments reveal how several factors, including age, influence estradiol's modulation of spatial navigation strategy use and suggest functional mechanisms by which this modulation occurs.</p> / Dissertation

This study investigates a phenomenon that I have termed occlusion inhibition. This research and a small number of earlier studies suggest that, in some experimental conditions, when an attended (target) object is partially occluded by a distractor object, there is less attention allocated to the occluded region of the target object than to the visible parts of that object. In the literature, there are mixed results concerning this attentional effect. Some studies find it and others do not. This study investigates the differences between those conflicting studies with the goal of identifying the factor or factors that govern when occlusion inhibition occurs. Evidence is presented to rule out a number of potentially relevant factors such as depth perception, figural complexity, set size, the use of real world vs. abstract geometric objects, the position of occlusion, the number of overlaps in the display, and the adoption of the attend-object paradigm over the spatial cueing paradigm. After all these factors are ruled out, Experiments 3 and 4 provide evidence for a factor that does determine whether occlusion inhibition occurs or not. These two experiments differ only in the fact that participants are required to report the border color of the target object in Experiment 3 and not in Experiment 4. This task was designed to ensure that participants fully attend to the target object. Occlusion inhibition occurs when the target color is reported, but not when no target color report is required. Removing the target reporting task was found to be an effective means of turning occlusion inhibition on and off. The results of these experiments suggest that, if occlusion inhibition is to take place, attentional selection of overlapping figures requires the target object to be fully processed. This conclusion in turn suggests that attention does not automatically exclude the irrelevant portions of occluded objects, but that attention selects the entire location of the object and then, through reiterative feedback mechanisms, fine tunes the information to inhibit areas that do not belong to the object.

There is a significant amount of computational literature on networks of neurons and their resulting behavior. This dissertation combines electrophysiology experiments with computational modeling to validate the assumptions and results found in this literature. First, we investigate the weak coupling assumption, which states that the phase response of a neuron to weak stimuli is separable from the stimulus waveform. For weak stimuli, there is an intrinsic neuronal property described by the infinitesimal phase response curve (IPRC) that will predict the phase response when convolved with the stimulus waveform. Here, we show that there is a linear relationship between the stimulus and phase response of the neuron, and that we are able to obtain IPRCs that successfully predict the neuronal phase response. Next, we use hybrid networks of neurons to study the phase locking behavior of networks as the synaptic time constant is changed. We verify that networks show anti-phase synchrony for fast time constants, and in-phase synchrony for slow time constants. We also show that phase models and phase response curves (PRCs) qualitatively predict phase locking observed in electrophysiology experiments. Finally, we investigate the stability of the dynamic clamp system. We determined that the maximal conductance of the current being simulated, the dynamic clamp sampling rate, the amount of electrode resistance compensation, and the amount of capacitance compensation all affect when the instability is present. There is a dramatic increase in stability when the electrode resistance and system capacitance are well compensated.

Movement is central for life, and all animals depend on accurate regulation of movement for purposeful behavior. There is great diversity of movements, ranging between simple and vital breathing movements to minute and subtle movements of the face used to communicate emotions. Consequently, motor neurons, which are the only route of central nervous system output, are essential for all motor behaviors. To control the many motor behaviors expressed by an animal, motor neurons are exposed to a large number and variety of modulating synaptic inputs and have evolved into subtypes with specific functions. In this thesis, motor neuron subtypes and the synaptic input to motor neurons from Renshaw cells and Ia afferents have been studied. Novel molecular markers that identify subtypes of motor neurons are described. Three markers, Chodl, Calca and ERRβ, have been used to study the degeneration of subtypes of motor neurons in a mouse model of the neurodegenerative disease amyotrophic lateral sclerosis (ALS). Another marker, 5-ht1d, has been used to record the electrophysiological character of gamma motor neurons. In mice that lack 5-ht1d, motor neurons develop with reduced proprioceptive input. Remarkably, these mice had fewer foot faults than control animals when challenged to cross a narrow beam suggesting that the amplitude of monosynaptic proprioceptive input to motor neurons is not essential for motor coordination. In a final set of experiments, genetic removal of vesicular transport of neurotransmitter from Renshaw cells suggest that Renshaw cells are not integral for motor circuit function or motor behaviors. However, they are involved in the development of motor circuits in the spinal cord. Together, this thesis provides novel molecular tools for studies of motor neuron subtypes and novel data regarding the development and function of spinal motor circuits.

The evolution, development and function of the embryonic and larval peripheral nervous system (PNS) were investigated in a number of dipteran species, including Aedes aegypti, Hermetia pallescens, Lucilia cuprina, Bactrocera tryoni and Drosophila melanogaster. Comparative immuno-cytochemical data was generated for developmental proteins in the embryonic PNS and degenerative PCR was employed to identify homologous proneural genes amongst the species. Immunocytochemistry revealed aspects of sense-organ evolution amongst the Diptera, particularly an increase in sense-cell number and number of sensilla comprising proprioceptive organs in the crawling versus swimming larvae. The function of putative proprioceptive cells was investigated via mutant analysis and laser ablation. Results indicate that the multiple-dendrite (md) and bipolar-dendrite (bd) neurons contribute in a cumulative and roughly equal fashion to maintain peristaltic waves in crawling larvae. This work highlights the usefulness of the dipteran PNS as a model of adaptive evolution that can be investigated via developmental mechanisms.

Thesis (M.A.)--University of North Carolina Wilmington, 2008. / Title from PDF title page (October 20, 2008) Includes bibliographical references (59-71)

Thesis (Ph. D.)--University of Sydney, 2008. / Title from title screen (viewed Mar. 12, 2008). Submitted in fulfilment of the requirements for the degree of Doctor of Philosophy to the Discipline of Medicine, Faculty of Medicine. Degree awarded 2008; thesis submitted 2007. Includes bibliography. Also issued in print.

Thesis (M.Sc.) - Carleton University, 2005. / Includes bibliographical references (p. 110-116). Also available in electronic format on the Internet.

Thesis (Ph. D.)--University of California, San Diego, 1999. / Vita. Includes bibliographical references (leaves 86-90).