Pearl millet repetitive

Ammonia and amino acid transport systems

Genetic and biochemical characterization

Ornithine decarboxylase

Regulation of chlorophyll biosynthesis, gene expression and organisation

Meat quality

I/D 59 -- General Council, December 2002 -- INTERNATIONAL AND INTERCULTURAL COMMUNITY LIFE -- 1. A new look for our family -- (pg. 1) -- 2.1 Benefits and advantages for mission and life together -- (pg. 2) -- 2.2 Benefits for the individual -- (pg. 4) -- 3. Challenges in international and intercultural communities -- (pg. 4) -- 4. Clarification of certain points -- (pg. 6) -- 5. Looking to the future -- (pg. 7) -- Conclusion -- (pg. 8) -- A LOVE THAT IS GENUINE – A Call to Integrity in our Relationships -- (pg. 1) -- Introduction -- (pg. 1) -- Consecrated Life: A Challenge to the Contemporary World -- (pg. 1) -- Formation -- (pg. 3) -- Role and Responsibility of Confreres in Leadership -- (pg. 5) -- Conclusion -- (pg. 8)

This study applied action research to explore the design and performances of the picture book teaching on the life education in order to understand learning effect on second graders¡¦ attitudes toward the life concerned self-confidence. The subjects in this study were second graders in an elementary school in Kaohsiung. This study designed the picture book teaching plan for life education, implementing 24 classes for a total of 8 weeks.Data-collecting methods was mainly depended on quanlitative data such as records of observation, interviews, learning sheets, teacher¡¦s reflection, parents and student feedback which were used in order to evaluate the effectiveness of the picture book on the life education teaching, and the teaching introspection and professional growth of the teacher. The main results of the study as following: 1. The teaching models would promote students abstract concepts during learning Life Education. 2. It concerned the impact on students after practicing the picture book instruction on the life education. (1) Students could respect life and understand themselves from various viewpoints and accept self. (2)By interacting with others, second graders can emerge themselves generously, and improve interpersonal relationship. (3) It was not obvious to students¡¦s being positivite and discovering uniqueness. 3. The teacher was promoted to the ability of designing curriculum and teaching skills of Life Education by having conversations with team-teachers. Finally,according to the research conclusions made concrete suggestions for teaching and future research.

Dihydrofolate reductase (DHFR) catalyzes the reduction of dihydrofolate (DHF) to tetrahydrofolate (THF) using NADPH as a cofactor. Since THF is an essential factor for nucleotide biosynthesis, inhibition of this enzyme in bacteria with folate analogs such as trimethoprim results in bacterial cell death. Plasmid encoded R67 DHFR confers resistance to trimethoprim and is both sequentially and structurally unrelated to any known chromosomal version of the enzyme. R67 DHFR is a 34,000 Da. homo-tetramer containing a rare 222 axis of symmetry in the center of its active site pore. The active site pore is contacted by residues belonging to each of the four subunits. The enzyme can nonspecifically bind 2 NADPH’s, 2 DHF’s, or, in the case of the productive complex, 1 NADPH and 1 DHF. R67 DHFR employs the endo transition state as opposed to the exo transition state used by E. coli DHFR. In this study molecular dynamics approach using the CHARMM program is employed to study the dynamics of the enzyme and energetics of the hydride transfer step catalyzed by R67 DHFR in silico. Structural and dynamic properties of four different mutants are also examined.

Crohn's disease is a chronic inflammatory bowel disease of unknown origin that continues to affect millions of people worldwide. Mycobacterium avium subsp. paratuberculosis (MAP) has been a suspected etiological agent of Crohn’s disease for nearly two decades. Recently, our laboratory was able to achieve a test sensitivity of 95.2% (n=21) by using a flow cytometry method (FCM) to detect anti-MAP antibodies in Johne’s diseased cattle. Here we investigate whether FCM can differentiate Crohn’s patients’ antibody titers from healthy human control antibody levels. As a second research goal, we investigated other suspected bacteria and serum-differentiating yeast using the FCM. In brief, bacteria/yeast were incubated with serum samples and washed to remove nonspecific antibodies. Antibodies bound to the surface of bacteria/yeast were then labeled with fluorescently-tagged secondary antibody and this binding evaluated by flow cytometry. Serum samples from 37 Crohn’s patients and 37 healthy human controls were tested for antibody (IgG and IgA) binding to bacteria/yeast. When targeting antibodies directed towards MAP, no significant difference was observed between the two populations (ANOVA, alpha=0.05). FCM was also unable to detect an antibody response for either group when directed against a cell-wall-deficient form of MAP, which has been reported to be near infected tissues of Crohn’s diseased patients. However, a differential antibody response to two yeasts that included S. cerevisiae and C. albicans was detected when looking for both IgG and IgA. This differentiation using yeast agrees with previous studies that have shown Crohn’s diseased subjects to have significantly higher antibody titers to this yeast than do healthy controls. This suggests that the flow cytometry method could be useful in the future for distinguishing Crohn’s subjects from healthy controls.