Previous studies have detected the presence of the capsid protein (CP) and CP RNA of PVX in chloroplasts of transgenic plants expressing the PVX CP and 8kD genes from nuclear transgenes. CP RNA was also found in the chloroplasts of transgenic plants with mutations that eliminated either the CP or the 8kD protein. To further investigate the potential “RNA Tractor” activity of the PVX RNA, various constructs containing limited regions of the PVX 8kD and CP genes were produced and used to transform tobacco plants. RT-PCR analyses of chloroplastic RNA ascertained the presence of the RNA transcript within chloroplasts when as little as 125bp of the PVX sequence was used to transform plants. From this, it was concluded that the PVX proteins did not contribute to the movement of the viral RNA and that a region within the 125bp PVX sequence is acting as a chloroplastic “RNA Tractor”.

Previous studies have detected the presence of the capsid protein (CP) and CP RNA of PVX in chloroplasts of transgenic plants expressing the PVX CP and 8kD genes from nuclear transgenes. CP RNA was also found in the chloroplasts of transgenic plants with mutations that eliminated either the CP or the 8kD protein. To further investigate the potential “RNA Tractor” activity of the PVX RNA, various constructs containing limited regions of the PVX 8kD and CP genes were produced and used to transform tobacco plants. RT-PCR analyses of chloroplastic RNA ascertained the presence of the RNA transcript within chloroplasts when as little as 125bp of the PVX sequence was used to transform plants. From this, it was concluded that the PVX proteins did not contribute to the movement of the viral RNA and that a region within the 125bp PVX sequence is acting as a chloroplastic “RNA Tractor”.

No description available.

No description available.

Les pulsateurs compacts sont des étoiles présentant des variations intrinsèques de luminosité dont les gravités de surface sont supérieures à 100,000 cm/s² On retrouve parmi ces objets deux familles des sous-naines chaudes de type B (sdB) pulsantes et quatre familles distinctes de naines blanches pulsantes. Dans le but d'observer les pulsations de tels objets pour ensuite analyser leur propriétés grâce à l'astéroséismologie, l'Université de Montréal, en collaboration avec le Imaging Technology Laboratory (ITL - University of Arizona), a développé la caméra Mont4K (Montreal4K) CCD qui est, depuis le printemps 2007, le principal détecteur employé au télescope Kuiper de 1.55 m du Mt Bigelow (Steward Observatory, University of Arizona). à l'aide de ce montage, des observations ont été menées pour quelques-uns de ces pulsateurs compacts. La première cible fut HS 0702+6043, un pulsateur hybride. Une importante mission pour cet objet, réalisée du 1er novembre 2007 au 14 mars 2008, a permis d'identifier 28 modes de pulsations pour cet objet en plus de mettre en évidence pour certains de ces modes d'importantes variations d'amplitude. Deux autres cibles furent les naines blanches pulsantes au carbone de type « Hot DQ » SDSS J220029.08-074121.5 et SDSS J234843.30-094245.3. Il fut possible de montrer de façon indirecte la présence d'un fort champ magnétique à la surface de J220029.08-074121.5 grâce à la présence de la première harmonique du mode principal. En outre, pour ces deux cibles, on a pu conclure que celles-ci font bel et bien partie de la classe des naines blanches pulsantes au carbone. / Compact pulsators are stars with surface gravities larger than 100,000 cm/s² showing intrisinc luminosity variations. Six different classes of such objects are known, including two pulsating hot subdwarf (sdB), and four pulsating white dwarf classes. In order to observe their pulsations and further analyze their properties through asteroseismology, the Universit\'e de Montr\'eal, in collaboration with the University of Arizona Imaging Instrument Laboratory, developed the Mont4K (Montreal4K) CCD camera which is the primary science detector of the Kuiper 1.55 m telescope at Mt Bigelow since Spring 2007. With this setup, several compact pulsators were targeted. The first target was the hybrid pulsator HS 0702+6043, observed from November 1st 2007 to March 14th 2008, for which 28 pulsation modes were isolated. Moreover, it was possible to highlight important amplitude variations in some of these modes. Two Hot DQ white dwarf pulsators, SDSS J220029.08-074121.5 and SDSS J234843.30-094245.3, were also targeted. In the case of J220029.08-074121.5, it was possible to highlight the presence of a strong magnetic field at its surface through the presence of the first harmonic of the main pulsation mode of this object. Furthermore, the follow-up campaigns on J220029.08-074121.5 and J234843.30-094245.3 allowed to classify these two white dwarf as Hot DQ pulsators.

Les pulsateurs compacts sont des étoiles présentant des variations intrinsèques de luminosité dont les gravités de surface sont supérieures à 100,000 cm/s² On retrouve parmi ces objets deux familles des sous-naines chaudes de type B (sdB) pulsantes et quatre familles distinctes de naines blanches pulsantes. Dans le but d'observer les pulsations de tels objets pour ensuite analyser leur propriétés grâce à l'astéroséismologie, l'Université de Montréal, en collaboration avec le Imaging Technology Laboratory (ITL - University of Arizona), a développé la caméra Mont4K (Montreal4K) CCD qui est, depuis le printemps 2007, le principal détecteur employé au télescope Kuiper de 1.55 m du Mt Bigelow (Steward Observatory, University of Arizona). à l'aide de ce montage, des observations ont été menées pour quelques-uns de ces pulsateurs compacts. La première cible fut HS 0702+6043, un pulsateur hybride. Une importante mission pour cet objet, réalisée du 1er novembre 2007 au 14 mars 2008, a permis d'identifier 28 modes de pulsations pour cet objet en plus de mettre en évidence pour certains de ces modes d'importantes variations d'amplitude. Deux autres cibles furent les naines blanches pulsantes au carbone de type « Hot DQ » SDSS J220029.08-074121.5 et SDSS J234843.30-094245.3. Il fut possible de montrer de façon indirecte la présence d'un fort champ magnétique à la surface de J220029.08-074121.5 grâce à la présence de la première harmonique du mode principal. En outre, pour ces deux cibles, on a pu conclure que celles-ci font bel et bien partie de la classe des naines blanches pulsantes au carbone. / Compact pulsators are stars with surface gravities larger than 100,000 cm/s² showing intrisinc luminosity variations. Six different classes of such objects are known, including two pulsating hot subdwarf (sdB), and four pulsating white dwarf classes. In order to observe their pulsations and further analyze their properties through asteroseismology, the Universit\'e de Montr\'eal, in collaboration with the University of Arizona Imaging Instrument Laboratory, developed the Mont4K (Montreal4K) CCD camera which is the primary science detector of the Kuiper 1.55 m telescope at Mt Bigelow since Spring 2007. With this setup, several compact pulsators were targeted. The first target was the hybrid pulsator HS 0702+6043, observed from November 1st 2007 to March 14th 2008, for which 28 pulsation modes were isolated. Moreover, it was possible to highlight important amplitude variations in some of these modes. Two Hot DQ white dwarf pulsators, SDSS J220029.08-074121.5 and SDSS J234843.30-094245.3, were also targeted. In the case of J220029.08-074121.5, it was possible to highlight the presence of a strong magnetic field at its surface through the presence of the first harmonic of the main pulsation mode of this object. Furthermore, the follow-up campaigns on J220029.08-074121.5 and J234843.30-094245.3 allowed to classify these two white dwarf as Hot DQ pulsators.