Orientador: Marcius Cesar Soares Freire / Dissertação (mestrado) - Universidade Estadual de Campinas, Instituto de Artes / Made available in DSpace on 2018-08-12T16:11:49Z (GMT). No. of bitstreams: 1 Modenese_Luciane_M.pdf: 8358177 bytes, checksum: f88dc682c27df29a1fc7d0a7092ddd5c (MD5) Previous issue date: 2008 / Resumo: Este trabalho propõe a análise da obra documental do diretor Walter Salles, através de quatro documentários produzidos entre 1987 e 2002. Para tanto, discute cada um desses filmes de maneira a encontrar traços que caracterizem o modo com que o diretor realiza as narrativas no gênero. / Abstract: This dissertation proposes the analysis of the documentary work of director Walter Salles, through four documentaries produced between 1987 and 2002. It discusses each of these films in order to find aspects that characterize the way in which the director performs the narratives in the type concerned. / Mestrado / Mestre em Multimeios

Orientador : Luiz Sebastião Prigenzi / Dissertação (mestrado) - Universidade Estadual de Campinas, Instituto de Biologia / Made available in DSpace on 2018-07-14T03:26:16Z (GMT). No. of bitstreams: 1 Barros-Mazon_Silviade_M.pdf: 6522018 bytes, checksum: 98dabf9a4f442e09fa15004e4d479865 (MD5) Previous issue date: 1988 / Resumo: A partir de uma amostra de tecido mamário tumoral obteve-se dois fragmentos, denominados respectivamente fragmento 1 e fragmento 2, os quais foram submetidos independentemente a dois diferentes processos de fracionamento proteico, com o obJetivo de se comparar seus produtos resultantes. O primeiro processo de fracionamento, que foi aplicado ao fragmento 1, constituiu-se de cromatografia de exclusão (Bio-Gel A-50), seguida de cromatografia de troca iônica (DEAE - celulose) e resultou na eluição de quatro frações, denominadas respectivamente: FI/T; FII/T; FIII/T e FIV/T. Uma amostra de tecido mamário normal também foi submetida a este processo, tendo como resultantes as frações: FI/N e FII/N. O fragmento 2 foi submetido ao segundo processo de fracionamento, representado pelo emprego de cromatografia de afinidade (WGA - Sepharose 6 M8), que emitiu a obtenção da fração única, denominada F - NAG. Para efeito de analise comparativa, as diferentes frações protéicas foram submetidas: i) a analise eletroforetica em gel de poliacrilamida, na presença de SDS; 2) a analise imunoqu{mica, através de imunodifusio dupla e imunoeletroforese; 3) a analise do potencial de ligação lectina WGA_125I. A analise eletrofortica demonstrou a presença de vários componentes na fração FII/T, nio detectados nas fraç3es resultantes de tecido mam~rio normal, FI/N e FII/N. Alguns desses componentes encontraram correspondência na fração F-NAG, resultante da cromatografia de afinidade. Através da analise por imunodifusio foi possível detectar a presença de .componentes possivelmente associados ao tumor mamario, nas frações FI/T, FII/T, FIV/T e F-NAG. A analise imunoeletroforetica destas fraç3es permitiu esclarecer que o componente presente na fração F-NAG, com mobilidade 'alfa¿2 era distinto do componente aparentemente comum presente nas demais fraç3es, cuJa mobilidade era 'beta¿2. A analise do potencial de ligação WGA-125I revelou que somente a fração F-NAG continha componentes acessíveis ~ ligação com a lectina / Abstract: Not informed. / Mestrado / Mestre em Ciências Biológicas

No description available.

In ultrasonic processing, it has been a practice to measure intensity of ultrasonics as a product of plate current and voltage as indicated on ultra sonic generator. At low frequencies the standard equipment is available, such as the sound level meter, to measure the absolute intensity, but at higher frequencies the problem becomes complicated by the diffraction effects on account of the size of the measuring device. In this investigation an attempt has been made to construct a measuring device (called the probe) to measure intensity. A small barium titanate cylindrical element (1/16 inch in diameter and 1/16 inch length) was used as a detector-convertor of sonic pressures. The alternating voltages thus produced were indicated by a vacuum tube voltmeter. This element was cemented in a hypodermic needle in turn was held in a lucite rod (1/2 inch in diameter and five inches in length) which served as a holder. This arrangement was used to probe the sonic fields of cylindrical and bowl shaped transducers. / Ph. D.

Early interest concerning the control of the green June beetle, Cotinis nitida Linn., also called the fig-eater, was recorded during the middle of the nineteenth century. Since that time farmers in Virginia and other Southeastern States have been trying to find better methods for the control of this insect. The larvae of this beetle, . en present in large numbers, are capable of almost completely destroying certain forage crops such as alfalfa and clover, not by direct feeding, but from the results of their search for food. The adult, although not of prime economic importance in this region, does cause considerable economic losses. It feeds on fruits from a number of different species of plants such as grapes, peaches, raspberries, apples, pears, plums and in particular figs, hence the common name fig-eater. A definite affinity also is shown towards certain grains such as corn and sorghum. / Master of Science

The NAD glycohydrolase (NADase) from Bungarus fasciatus venom was purified over 1000-fold to electrophoretic homogeneity through a 3-step procedure which included affinity chromatography on Cibacron Blue agarose. The enzyme exhibited a broad pH profile with the optimum range between 7-8. Studies on the substrate specificity of B. fasciatus venom NADase demonstrated that alterations in the purine ring were less pronounced then alterations in the pyridinium moiety of NAD. Product inhibition studies indicated nicotinamide to be a noncompetitive inhibitor with a K_i = 1.4 mM and ADP-ribose to be a competitive inhibitor with a K_i =0.4 mM. The purified enzyme was inactivated by both 2,4-pentane dione and Woodward's Reagent K suggesting the involvement of a lysine and carboxyl group in the catalytic process. In contrast to other known NADases, the snake venom enzyme did not self-inactivate. The purified B. fasciatus venom NADase catalyzed a transglycosidation reaction (ADP-ribose transfer) with a number of acceptor molecules. The functioning of a variety of substituted pyridine bases as acceptor molecules was demonstrated through the formation of the corresponding NAD analogs. The enzyme also catalyzed the transfer of ADP-ribose to aliphatic alcohols (methanol to hexanol, inclusive) and a positive chainlength effect was observed in the functioning of these acceptors. Kinetic studies of transglycosidation reactions were consistent with the partitioning of an enzyme-ADP-ribose intermediate between water and nucleophilic acceptors as has been proposed in earlier studies of mammalian NADases. The partitioning of this intermediate between water and pyridine bases can be correlated with the basicity of the ring nitrogen of the pyridine derivative. The K_i of pyridine bases in the hydrolytic reaction did not equate to the K_m of these bases in the pyridine base exchange reaction suggesting two forms of the NADase with varying affinity for the pyridine bases. This implys the pyridine base exchange reaction to be more complicated than originally proposed. / Master of Science

The purpose of this thesis project was to design and construct a free surface water table for the investigation of compressible-flow phenomena. Although the design range of operation of the water table was to include all desired analogous Mach numbers between 0.40 and 7.00, the range of operation was limited by the formation of unwanted waves at the entrance section. With the application of elastic glazing compound to provide a rounded entrance and with the addition of a wetting agent to reduce the surface tension of the water, the water table performed satisfactorily at analogous Mach numbers between 0.40 and 4.00. The test section depth could not be easily varied because it was necessary to fill the grooves of the gate guides, with elastic glazing compound, for each gate opening. Except for the entrance section, very satisfactory performance was attained with all the components of the water table. Satisfactory performance of the water table was verified by the flow photographs and the results of the representative tests. / Master of Science

As a suggestion for further work in deriving the equations by the Principle of Virtual Displacements, it might be advisable to write the equations based on the vertical displacements of the points of inflection of each column. The concentrated mass of each panel would be located at the center of the respective columns. This appears to be a better approximation than concentrating each mass at the lower joint. / Master of Science

The purpose of the investigation was to determine if coliform organisms isolated on membrane filters can be incubated at a humidity below the point of saturation. The investigation consisted of comparing the development of coliform organisms on membrane filters incubated at various controlled levels of humidity with the development of coliform organisms incubated in a saturated atmosphere. The effect of humidity on various types of differentiating media was considered simultaneously with the above investigation. Several suggested methods for the regulation of humidity were examined and evaluated and two new methods were developed. The final results indicate that it is possible to incubate coliform organisms on membrane filters at all levels of humidity above 35 percent saturation. There was no significant difference noted in the results obtained on the four differentiating mediums: Two Step Endo, Single Step Endo, Hajna-Damon Endo and Dehydrated Schedule Nutrient. Gooch tubing provides a more nearly watertight seal in the membrane filter procedure than either Parafilm or Plastic Disposable Petri dishes. The relative humidity of a standard incubator may be regulated to any desired level up to about 85 percent by exposing a definite water surface area per unit volume of incubator space. This procedure permits ordinary incubators to be used for membrane filter examinations. / Master of Science

Primary Objective: To find a medium, defined as clearly as possible in terms of its nitrogenous components, which will support growth of a cellulolytic rumen bacterium. Secondary Objectives: 1. To test an hypothesis that the optimum nitrogenous nutrient balance for a strongly heterotrophic organism is that mixture of the basic structural molecules ( amino acids, purines, and pyrimidines ) which most closely approximates the composition of the organism itself. 2. To apply this hypothesis toward the goal set in the first objective. / Master of Science