Recognition that the bioavailability of soil As (As) is influenced by its soil dynamics has initiated research into development of more accurate, site-specific soil guideline values, departing from the assumption that the total soil As content is bioavailable. With the aim of deriving predictive models, the relationship between soil properties and As bioavailability (bioaccessibility and phytotoxicity) was examined on a set of naturally contaminated sheep dip soils (n = 30). Sampled soils were extensively characterised, bioaccessibility was estimated through an in vitro procedure, and soil As toxicity and availability to plants were evaluated using an early growth wheat bioassay. The in vitro bioaccessibility was consistently less than the total soil As content. Arsenic bioaccessibility was negatively correlated to soil iron (Fe), manganese (Mn) and aluminium (Al) contents, and it was positively related to the soil As loading. The in vitro extractable soil As concentrations were successfully modelled using linear combinations of soil As content, soil Fe and Mn determinations and soil pH. Differences in As phytotoxicity, expressed in terms of effective toxic concentration (EC50), between soils were directly related to soil Fe, Mn and Al contents. Available soil phosphorous (P) exerted an ameliorating effect on As toxicity, with the available soil As/P ratio representing the single best predictor of plant growth suppression. Plant P nutrition appeared to influence the relative selectivity for As and P by wheat, with greater selectivity for P demonstrated under P deficient conditions. Plant As uptake, its distribution, and also the plant nutrient status were all adversely affected by increasing soil As exposure. Co-contamination by Zn corresponded to a substantial elevation in proportion of the plant As allocated in shoots. Plant As levels exhibited a saturation-dependent relationship with increasing soil As. The best linear predictors of plant As levels in the non-toxic range were RHIZO-extractable and effective soil As concentrations, the latter based on the diffusive gradients in thin films (DGT) technique. Despite the complexity of soil As dynamics, large proportions in the variances exhibited by the two measures of bioavailability were explained using a small set of readily-available soil properties.

Recent declines in returns from primary forest products in New Zealand and projected increases in world food prices have led to the land-use conversion from plantation forest to pastoral farming in many lowland areas. After decades of forest cover the soils are in many cases less than adequate for pastoral farming, as they are acidic, with toxic levels of exchangeable aluminum, and contain low levels of available nitrogen (N), very high carbon (C):N ratio, and are devoid of earthworms and structural integrity. Overcoming the major site limitations of low soil pH and available N was a major priority and a field experiment was established in April 2005 to determine the impact of various rates of lime and N in relation to pasture establishment and production. Concerns about the short and long-term effects of these inputs on biological soil quality gave rise to the present study. The effects of land-use change and establishment inputs were assessed by comparison of selected treatment plots with two adjacent reference sites (long-term pasture and a 60–year Pinus radiata forest) on the same soil type. The effects of lime and N on soil biological quality were investigated under field and controlled environment conditions by determination of: microbial community structure (phospholipid fatty acids - PLFA), microbial biomass (total PLFA), and microbial activity (dehydrogenase activity). Soil physical (percentage water-stable aggregates) and chemical (pH, and total C and N) properties were also determined. Similarly, the effects of earthworm addition on soil biological properties were explored in a short-term glasshouse pot experiment. The role of earthworms as indicators of soil biological quality in the field was assumed by nematodes and these were assessed in field trial plots and the reference sites mentioned above. Land-use change and applications of lime and N contributed to changing the microbial community structure determined by principal component analysis of transformed PLFA data. However, the effect of lime was more pronounced in the field, while N contributed most to changing microbial community structure in the glasshouse. Mean microbial activity in the field increased from 4 µg dwt/hr without lime to 16 and 21 µg dwt/hr where lime was applied at 5 and 10 tons/hectare (t/ha), respectively. Mean microbial activity in the field was markedly higher (7-fold) than in the glasshouse at similar rates of lime. Lime application also increased soil moisture retention in the field, mean gravimetric soil moisture increased from 0.33 in control plots to 0.38 and 0.39 in plots treated with 5 and 10 t/ha lime, respectively. Lime application was associated with greater soil aggregate stability. Soils from test plots treated with 5 and 10 tons/ha lime had 45-50% water-stable aggregates compared to 34% in treatments without lime. After 16 weeks in pots, earthworm treatments increased mean plant dry matter (DM)/pot by at least 19% above the control. The increase was attributed primarily to greater N mineralization in the presence of earthworms. For the duration of the trial the earthworm species tested (Apporectodea caliginosa and Lumbricus rubellus, individually or combined) did not affect any of the measured soil microbial properties. However, the survival rate of A. caliginosa was 83% compared to 25% for L. rubellus. The control not receiving any lime or N and plots treated with 10t/ha lime and 200 kgN/ha had similar nematodes species composition, comprising 40% each of bacterial and fungal feeding nematodes. They differed markedly from the reference sites as the forest soil was dominated by plant associated species (38%) and the long-term pasture had 44% plant parasitic nematodes. Accordingly, the soil food web condition inferred from nematode faunal analysis characterized all test plots as basal, stressed and depleted, while the forest soil was categorized as highly structured and fungal dominated. The findings of this thesis demonstrated that land-use change from forest to pasture can have significant impacts on soil biological properties, earthworms can contribute to pasture productivity even in the short term, and nematode faunal analysis is a robust and reliable indicator of soil biological quality.

Recent declines in returns from primary forest products in New Zealand and projected increases in world food prices have led to the land-use conversion from plantation forest to pastoral farming in many lowland areas. After decades of forest cover the soils are in many cases less than adequate for pastoral farming, as they are acidic, with toxic levels of exchangeable aluminum, and contain low levels of available nitrogen (N), very high carbon (C):N ratio, and are devoid of earthworms and structural integrity. Overcoming the major site limitations of low soil pH and available N was a major priority and a field experiment was established in April 2005 to determine the impact of various rates of lime and N in relation to pasture establishment and production. Concerns about the short and long-term effects of these inputs on biological soil quality gave rise to the present study. The effects of land-use change and establishment inputs were assessed by comparison of selected treatment plots with two adjacent reference sites (long-term pasture and a 60–year Pinus radiata forest) on the same soil type. The effects of lime and N on soil biological quality were investigated under field and controlled environment conditions by determination of: microbial community structure (phospholipid fatty acids - PLFA), microbial biomass (total PLFA), and microbial activity (dehydrogenase activity). Soil physical (percentage water-stable aggregates) and chemical (pH, and total C and N) properties were also determined. Similarly, the effects of earthworm addition on soil biological properties were explored in a short-term glasshouse pot experiment. The role of earthworms as indicators of soil biological quality in the field was assumed by nematodes and these were assessed in field trial plots and the reference sites mentioned above. Land-use change and applications of lime and N contributed to changing the microbial community structure determined by principal component analysis of transformed PLFA data. However, the effect of lime was more pronounced in the field, while N contributed most to changing microbial community structure in the glasshouse. Mean microbial activity in the field increased from 4 µg dwt/hr without lime to 16 and 21 µg dwt/hr where lime was applied at 5 and 10 tons/hectare (t/ha), respectively. Mean microbial activity in the field was markedly higher (7-fold) than in the glasshouse at similar rates of lime. Lime application also increased soil moisture retention in the field, mean gravimetric soil moisture increased from 0.33 in control plots to 0.38 and 0.39 in plots treated with 5 and 10 t/ha lime, respectively. Lime application was associated with greater soil aggregate stability. Soils from test plots treated with 5 and 10 tons/ha lime had 45-50% water-stable aggregates compared to 34% in treatments without lime. After 16 weeks in pots, earthworm treatments increased mean plant dry matter (DM)/pot by at least 19% above the control. The increase was attributed primarily to greater N mineralization in the presence of earthworms. For the duration of the trial the earthworm species tested (Apporectodea caliginosa and Lumbricus rubellus, individually or combined) did not affect any of the measured soil microbial properties. However, the survival rate of A. caliginosa was 83% compared to 25% for L. rubellus. The control not receiving any lime or N and plots treated with 10t/ha lime and 200 kgN/ha had similar nematodes species composition, comprising 40% each of bacterial and fungal feeding nematodes. They differed markedly from the reference sites as the forest soil was dominated by plant associated species (38%) and the long-term pasture had 44% plant parasitic nematodes. Accordingly, the soil food web condition inferred from nematode faunal analysis characterized all test plots as basal, stressed and depleted, while the forest soil was categorized as highly structured and fungal dominated. The findings of this thesis demonstrated that land-use change from forest to pasture can have significant impacts on soil biological properties, earthworms can contribute to pasture productivity even in the short term, and nematode faunal analysis is a robust and reliable indicator of soil biological quality.

This thesis aIms to produce a distinctive model for the sustainable horticultural development of Maori resources, primarily land. It is inclusive of tikanga Maori and indigenous production systems based on the unique body of knowledge aligned to Maori. The integration of this knowledge with western science is both argued and applied through the model itself. The hypothesis applied was that matauranga Maori relevant to horticulture and pedology can inform and add value to the future development of Maori land resources. The thesis is bui lt on a unique set of contributing knowledge bases aligned to soils and horticultural management supported by three case studies, identified through their common association i.e. whakapapa links. The format of the thesis intentionally follows science principles in structure and presentation and some assumptions are made regarding base knowledge surrounding Maori cultural factors and the science disciplines relative to soils and horticul ture. The indigenous element, including Maori knowledge, is incorporated into the model using a triadic kosmos/corpus/praxis approach. Where kosmos is applied as Te A o Miiori, corpus as miitauranga Miiori and praxis as tikanga Miiori, the relationship between each element is clear and the interpretation of the associated knowledge becomes more apparent and can be applied to cultural assessments of resources, i ncluding land. The crux of the cultural assessment model is the quality of information used to assess Maori resources, especially from the cultural perspective. The Maori cultural paradigm, traditional horticulture and pedology, and various decision systems are purposefully accessed to act as contributors to the assessment model and to highlight the diversity and quality of information land managers have at their disposal. The ability to apply a cultural layer drawn from a body of knowledge not previously included in decision models relative to land utility in New Zealand is the key point. of difference of the model. The model is discussed from the perspective of its beneficial role for future use by Maori and how it can be continuously refined to meet the needs of Maori land owners and thus contribute to the rangatiratanga of Maori.

The Participatory Irrigation Management approach was introduced into Cambodia in 2000, which was called the Participatory Irrigation Management and Development (PIMD). The goal of PIMD is to establish Farmer Water User Communities (FWUCs) to take over the management of irrigation schemes in their district in order to improve the performance of irrigation schemes and farmers’ livelihoods. The implementation of FWUCs has resulted in both failure and success. Several studies have identified factors that influence the failure of FWUCs, but little research has focused on their success. By employing a single embedded case study approach, this research selected the most successful scheme in Cambodia to identify factors that influenced the success of the FWUC in irrigation management. The findings of this research could provide concrete assistance to the government, donors, and non-governmental organisations in improving the performance of less successful FWUCs in Cambodia. The result of this research showed that the success of the O-treing FWUC was influenced by five internal and two external factors. The internal factors were: 1) the level of local participation, 2) the governance and management of the scheme, 3) the value of the benefits that flow from the irrigation scheme, 4) the quality of the irrigation infrastructure, and 5) the characteristics of the farmer members within the scheme. The external factors were: 1) the level of external support provided to the scheme, and 2) market access. The success of the FWUC required farmer participation and this participation was enhanced when farmers obtained benefits from it. This research also found that access to markets was critical to make the benefits that flowed from the irrigation scheme more profitable to farmers, leading to farmer participation. Similarly, it was also important to make sure that the irrigation infrastructure was of a high quality to ensure the delivery of an adequate and timely supply of water to farmers so that they could grow crops that provided them with the benefits. This required external support from the Ministry of Water Resources and Meteorology, NGOs, and local authorities to help rehabilitate the scheme. External support was also critical for enhancing the governance and management of the scheme through assistance with the formation process, provision of financial resources, capacity building, rule enforcement, and conflict resolution. The governance and management of the scheme, in particular the leadership capacity of the FWUC was another critical factor because it ensured the maintenance and development of the irrigation infrastructure, the timely and adequate supply of water to farmers, farmers’ trust and respect for leaders, and farmer participation. Finally, the success of the FWUC could not be viewed independently from farmer characteristics within the scheme. Farmers tended to participate in irrigation management when they had a history of self-organisation, when they were relatively homogenous, and when they were dependent upon farming for their livelihoods. This research suggests that the successful implementation of FWUCs requires a focus on the seven factors and the interactions that occur between these factors. Irrigation stakeholders such as the Ministry of Water Resources and Meteorology, donors, NGOs, local authorities, local leaders, and farmers should work together to enhance these factors in order to ensure the success of FWUCs.

The last decade has been a period of great expansion and land use intensification for the New Zealand dairy farming industry with a 44% increase in national dairy cow numbers. Intensive dairy farming is now considered to be a major contributor to the deterioration in the quality of surface and ground water resources in some regions of New Zealand. Previous research has demonstrated intensive dairy farming is responsible for accelerated contamination of wateways by nutrients, suspended solids, pathogenic organisms and faecal material. A number of common dairy farming practices increase the risk of nutrient leaching. In particular, farm dairy effluent (FDE) has been implicated as a major contributor to the degradation of water quality. With the introduction of the Resource Management Act in 1991, the preferred treatment for FDE shifted away from traditional two-pond systems to land application. However, on most farms, irrigation of FDE has occurred on a daily basis, often without regard for soil moisture status. Therefore, it has been commonplace for partially treated effluent to drain through and/or runoff soils and contaminate fresh water bodies. The objectives of this thesis were to design and implement a sustainable land application system for FDE on difficult to manage, mole and pipe drained soils, and to assess the impacts of FDE application, urea application and cattle grazing events on nutrient losses via artificial drainage and surface runoff from dairy cattle grazed pasture. To meet these objectives a research field site was established on Massey University's No.4 Dairy farm near Palmerston North. The soil type was Tokomaru silt loam, a Fragiaqualf with poor natural drainage. Eight experimental plots (each 40 x 40 m) were established with two treatments. Four of the plots represented standard farm practice including grazing and fertiliser regimes. Another four plots were subjected to the same farm practices but without the fertiliser application and they were also irrigated with FDE. Each plot had an isolated mole and pipe drainage system. Four surface runoff plots (each 5 m x 10 m) were established as subplots (two on the fertilised plots and two on the plots irrigated with FDE) in the final year of the study. Plots were instrumented to allow the continuous monitoring of drainage and surface runoff and the collection of water samples for nutrient analyses. An application of 25 mm of FDE to a soil with limited soil water deficit - simulating a 'daily' irrigation regime - resulted in considerable drainage of partially treated FDE. Approximately 70% of the applied FDE left the experimental plots with 10 mm of drainage and 8 mm of surface runoff. The resulting concentrations of N and P in drainage and runoff were approximately 45% and 80% of the original concentrations in the applied FDE, respectively. From this single irrigation event, a total of 12.1 kg N ha-1 and 1.9 kg P ha-1 was lost to surface water representing 45% of expected annual N loss and 100% of expected annual P loss. An improved system for applying farm dairy effluent to land called 'deferred irrigation' was successfully developed and implemented at the research site. Deferred irrigation involves the storage of effluent in a two-pond system during periods of small soil moisture deficits and the scheduling of irrigation at times of suitable soil water deficits. Deferred irrigation of FDE all but eliminated direct drainage losses with on average <1 % of the volume of effluent and nutrients applied leaving the experimental plots. Adopting an approach of applying 'little and often' resulted in no drainage and, therefore, zero direct loss of nutrients applied. A modelling exercise, using the APSlM simulation model, was conducted to study the feasibility of practising deferred irrigation at the farm scale on No 4 Dairy farm. Using climate data for the past 30 years, this simulation exercise demonstrated that applying small application depths of FDE, such as 15 mm or less, provided the ability to schedule irrigations earlier in spring and decreased the required effluent storage capacity. A travelling irrigator, commonly used to apply FDE (a rotating irrigator), was found to have 2-3 fold differences in application depth and increased the risk of generating FDE contaminated drainage. New irrigator technology (an oscillating travelling irrigator) provided a more uniform application pattern allowing greater confidence that an irrigation depth less than the soil water deficit could be applied. This allowed a greater volume to be irrigated, whilst avoiding direct drainage of FDE when the soil moisture deficit is low in early spring and late autumn. A recommendation arising from this work is that during this period of low soil water deficits, all irrigators should be set to travel at their fastest speed (lowest application depth) to minimise the potential for direct drainage of partially treated FDE and associated nutrient losses. The average concentrations of N and P in both 2002 and 2003 winter mole and pipe drainage water from grazed dairy pastures were all well above the levels required to prevent aquatic weed growth in fresh water bodies. Total N losses from plots representing standard farm practice were 28 kg N ha-1 and 34 kg N ha-1 for 2003 and 2004, respectively. Total P losses in 2003 and 2004 were 0.35 kg P ha-1 and 0.7 kg P ha-1, respectively. Surface runoff was measured in 2003 and contributed a further 3.0 kg N ha-1and 0.6 kg P ha-1. A number of common dairy farm practices immediately increased the losses of N and P in the artificial drainage water. Recent grazing events increased NO3--N and DIP concentrations in drainage by approximately 5 mg litre-1 and 0.1 mg litre-1, respectively. The duration between the grazing and drainage events influenced the form of N loss due to a likely urine contribution when grazing and drainage coincide, but had little impact on the total quantity of N lost. Nitrogen loss from an early spring application of urea in 2002 was minimal, whilst a mid June application in 2003 resulted in an increased loss of NO3--N throughout 80 mm of cumulative drainage suggesting that careful timing of urea applications in winter is required to prevent unnecessary N leaching. Storage and deferred irrigation of FDE during the lactation season caused no real increase in either the total-N concentrations or total N losses in the winter drainage water of 2002 and 2003. In contrast, land application of FDE using the deferred irrigation system resulted in a gradual increase in total P losses over the 2002 and 2003 winter drainage seasons. However, this increase represents less than 4% of the P applied in FDE during the lactation season. An assessment of likely losses of nutrients at a whole-farm scale suggests that it is standard dairy farming practice (particularly intensive cattle grazing) that is responsible for the great majority of N and P loss at a farm scale. When expressed as a proportion of whole-farm losses, only a very small quantity of N is lost under an improved land treatment technique for FDE such as deferred irrigation. The management of FDE plays a greater role in the likely P loss at a farm scale with a 5% contribution to wholefarm P losses from deferred irrigation.

This thesis aIms to produce a distinctive model for the sustainable horticultural development of Maori resources, primarily land. It is inclusive of tikanga Maori and indigenous production systems based on the unique body of knowledge aligned to Maori. The integration of this knowledge with western science is both argued and applied through the model itself. The hypothesis applied was that matauranga Maori relevant to horticulture and pedology can inform and add value to the future development of Maori land resources. The thesis is bui lt on a unique set of contributing knowledge bases aligned to soils and horticultural management supported by three case studies, identified through their common association i.e. whakapapa links. The format of the thesis intentionally follows science principles in structure and presentation and some assumptions are made regarding base knowledge surrounding Maori cultural factors and the science disciplines relative to soils and horticul ture. The indigenous element, including Maori knowledge, is incorporated into the model using a triadic kosmos/corpus/praxis approach. Where kosmos is applied as Te A o Miiori, corpus as miitauranga Miiori and praxis as tikanga Miiori, the relationship between each element is clear and the interpretation of the associated knowledge becomes more apparent and can be applied to cultural assessments of resources, i ncluding land. The crux of the cultural assessment model is the quality of information used to assess Maori resources, especially from the cultural perspective. The Maori cultural paradigm, traditional horticulture and pedology, and various decision systems are purposefully accessed to act as contributors to the assessment model and to highlight the diversity and quality of information land managers have at their disposal. The ability to apply a cultural layer drawn from a body of knowledge not previously included in decision models relative to land utility in New Zealand is the key point. of difference of the model. The model is discussed from the perspective of its beneficial role for future use by Maori and how it can be continuously refined to meet the needs of Maori land owners and thus contribute to the rangatiratanga of Maori.

Trifolium repens, Lotus pedunculatus and Holcus lanatus were oversown on two recent soil slip surfaces at AgResearch’s Ballantrae pastoral hill‐country farm near Woodville. The two slip surfaces were located on (Manamahu steepland soil) sedimentary mudstone. One slip had a north aspect and the other had a south aspect. Both slips were located on a land class 6 with slope 28‐330. The pasture species were oversown during early spring and the percentage seedling emergence and early establishment from viable seeds oversown was analysed at early spring (Day 15), late spring (Day 45), early summer (Day 90), and late summer (Day 120). The slip surfaces showed micro‐climatic extremes in terms of both soil moisture and surface temperatures during the summer period. Significant differences (P < 0.05) were found in soil moisture between north and south facing slip surfaces. Higher soil moisture and lower soil mean temperature were recorded on the south aspect slip surface. Significant differences (P < 0.05) were found between the three pasture species in terms of seedling emergence and early establishment. Significant differences (P < 0.05) were also found with aspect. The south aspect slip surface had a higher percentage of seedling emergence and earlier establishment for all the species. Interaction between species by aspect became significantly different (P < 0.05) at Day 90 and Day 120. The main effects of time and species were also significantly different (P < 0.05) illustrating seedling emergence and establishment as a race against time. Trifolium repens was a more successful pasture specie, than L. pedunculatus and H. lanatus due to its higher consistency on both north and south slip surfaces. Oversowing T. repens during early spring is a viable option for rehabilitation of recent soil slips in Manawatu.

The fate and behaviour of estrogens in the environment are of concern due to the compounds’ endocrine disruption potential. Estrogens, namely 17β-estradiol (E2), estrone (E1), and estrogen sulphates, i.e. 17β-estradiol-3-sulphate (E2-3S) and estrone-3-sulphate (E1-3S) excreted by livestock constitute a potential source for estrogen contamination in the environment. A method was developed to separate and quantify the hormones by high-performance-liquid-chromatography (HPLC) and ultraviolet detection (UV). A combination of dichloromethane (DCM) and dicyclohexylamine hydrochloride (DCH·HCl) gave recoveries from 97.3 to 107% for E1-3S extraction from aqueous solutions. The recoveries from soil samples ranged from 80.9 to 95.2% (E2-3S), and from 86.3 to 91.7% (E1-3S), respectively. Results of batch sorption studies showed that Freundlich isotherms were nonlinear (N ≠ 1) with Kf values ranging from 34.2 to 57.2, and from 3.42 to 4.18 mg¹-N LN kg⁻¹ for E1, and E1-3S, respectively, indicating the sorption affinity of E1-3S was about an order of magnitude lower than that of E1. The hydrophilic sulphate group of E1-3S possibly shielded the compound from hydrophobic interactions with the soil organic matter and allophanic clay minerals that were proposed as sorbents for E1. Contraction of clay minerals, “salting out” and competitive sorption of artificial urine constituents were likely to have been responsible for observed changes in Freundlich parameters when artificial urine was used as mediator matrix. Plotting the effective distribution coefficient as a function of hypothetical exposure concentrations facilitated the comparison of the sorption behaviour of both compounds as influenced by the mediator solution. The results emphasized that using the CaCl₂ matrix might result in false inferences for the sorption behaviour of these compounds in a dairying environment. The four hormones rapidly degraded in the agricultural soils under aerobic conditions, and the majority of the compounds degraded > 50% within the first 24 hrs. Soil arylsulphatase activities were directly correlated with degradation rate constants of the estrogen sulphates. Estrone was identified as a metabolite of E2 and E1-3S, and these three compounds were observed as metabolites of E2-3S. Single-first order (SFO) and double first-order in parallel (DFOP) kinetics were used to model the degradation and metabolite formation data. The results showed that the DFOP model was in most cases better able to predict the parent compound degradation than the SFO model, and also enabled to estimate accurate degradation endpoints. ER-CALUX® analysis revealed the formation of estrogenicity during E2-3S degradation, which could partly be explained by the formation of the metabolites E2 and E1. Transport studies with E1-3S and E1 showed that the transport and retention of both compounds were significantly influenced by the mediator matrix. While no breakthrough curves (BTCs) were recorded during hormone application in CaCl₂ (10 mM) both hormones were detected in the leachate when applied in artificial urine. Rate-limited sorption processes were proposed for the delayed arrival of the hormone BTCs compared with a conservative bromide tracer. Intense colouration of the leachate during the artificial urine experiments suggested the hormones were likely to be moved by colloid-facilitated transport. Furthermore, the detection of residue hormone and metabolite concentrations implied that degradation of E1-3S and E1 was hampered by urine constituents such as glycine and urea.

The overall aim of this research was to improve the understanding of the ecology and diversity of Trichoderma species within the soil and rhizosphere of onion (Allium cepa L.) and potato (Solanum tuberosum L.) under intensive management in New Zealand. The indigenous Trichoderma population was measured in a field trial at Pukekohe over a three year period under six different crop rotation treatments. The treatments included two continuous onion and potato rotations (intensive), two onion/potato mixed rotation (conventional), and two green manure rotations (sustainable). Results showed that Trichoderma populations were stable in both the rhizosphere and bulk soil (1.5 x 10² to 8.5 x 10³ CFU g⁻¹ ODS). The planting and incorporation of an oat (Avena sativa L.) green manure in the sustainable rotations positively increased Trichoderma colony forming unit (CFU) numbers in the rhizosphere soil from 3.4 x 10² to 2.5 x 10³ g⁻¹ ODS. A Trichoderma species identification method was developed based on colony morphology. Representative isolates were verified using restriction fragment length polymorphism (RFLP) and DNA sequencing. The method allowed for rapid and reliable identification of isolated Trichoderma species. Five species were identified in the Pukekohe soil: T. asperellum, T. atroviride, T. hamatum, T. harzianum and T. koningii. Results showed identical species diversity between the rhizosphere, rhizoplane and bulk soil. The species did not strongly compete between each other for the rhizosphere ecological niche and differences in species proportions seemed to be caused by environmental factors rather than the rotation treatments. The incorporation of oat green manure in pots did not significantly promote the indigenous Trichoderma population size and diversity in the rhizosphere of onion plants up to 4 months old. The identified species were the same as in the field trial. The incorporation of onion scale residues was shown to result in low Trichoderma and high Penicillium CFU numbers and a reduction in plant size. Additionally, the presence of high levels (6.0 x 10⁵ CFU g⁻¹ ODS) of Penicillium CFU was negatively correlated with the presence of Trichoderma CFU. The effect of oat incorporation on Trichoderma saprophytic growth was also investigated in a soil sandwich assay and revealed no significant differences. A series of experiments indicated that onion extract obtained from dry onion scale residues had no antifungal activity against either Trichoderma or Penicillium and instead tended to promote their hyphal growth and sporulation. It also showed that competition between Penicillium and Trichoderma isolates was limited despite the ability of Penicillium to produce a wide range of inhibitory substances. Four indigenous Trichoderma species (T. atroviride, T. hamatum, T. harzianum and T. koningii) were shown to be rhizosphere competent in a split tube experiment over a 6 week period. The results of this experiment revealed that, the Trichoderma species clearly displayed differences in their ability to colonise the rhizosphere of young onion seedlings. Species such as T. koningii had the greatest rhizosphere colonising ability regardless of soil depth while T. harzianum displayed the weakest ability. Results also indicated that when inoculated as a mixture the four species competed with one another to colonise the rhizosphere. Overall, this research indicated that the studied crop rotation treatments and the use of oat as a green manure did not strongly promote indigenous Trichoderma populations. Species diversity was constant throughout the research with T. hamatum and T. koningii being the most frequently isolated species.