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The Global ETD Search service is a free service for researchers
to find electronic theses and dissertations. This service is
provided by the
Networked Digital Library of Theses and Dissertations.
Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
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Showing 261 to 270 of 11567 (0.022 seconds)| 261 |
Kinase-anchoring proteins and the intracellular targeting of cyclic adenosine monophosphate (cAMP)-dependent protein kinaseDavis, Alison Jane January 2007 (has links)
Kinase anchoring proteins (AKAPs) are a family of structurally diverse multidomain proteins classified solely by their ability to bind cAMP-dependent protein kinase/protein kinase A (PKA). We show that the prototypic AKAP, AKAP79, targets PKA to the plasma membrane. This subcellular localisation is mediated via interactions between basic regions on AKAP79 and acidic phospholipids in the plasma membrane. We show that while PKA and protein kinase C (PKC)-dependent phosphorylation of AKAP79 targeting residues has no effect on its subcellular localization, Gq-coupled receptor-driven depletion of the lipid anchor PtdIns(4,5)P2 causes release of AKAP79 from the membrane. Receptor-mediated regulation of AKAP membrane binding may be an important feedback mechanism controlling the degree of access that AKAP-bound enzymes have to membrane-associated substrates. Real-time confocal imaging failed to show significant redistribution of AKAP79 following Gq-coupled receptor activation, which may reflect only limited short-range movements.;In addition to regulated association with membrane, we also show through co-immunoprecipitation studies a direct interaction between AKAP79 and all three members of the inwardly rectifying potassium channel Kir2 family. Using a combination of deletion analysis and structural modelling we show that AKAP79 binds the Kir2 family via a novel binding motif located on the C terminus of the ion channel and not via a leucine zipper as has been reported for other AKAP-channel interactions. Interestingly, we also show using a PKA regulatory subunit overlay assays that this region of Kir2.1 is capable of isolating other, potentially novel, AKAPs from rat brain and heart homogenates.;Finally, we use whole-cell patch clamp analysis to demonstrate that cAMP-dependent modulation of Kir2.1 channel activity is significantly enhanced in the presence of AKAP79, thus confirming an important role for AKAP79 in targeting PKA to key channel phosphorylation sites.
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An investigation of the active site of papainClark, P. I. January 1978 (has links)
No description available.
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Cloning and regulation of the rat bradykinin B1 receptor geneJones, Caroline Jane January 2000 (has links)
No description available.
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The teichoic acids from the cell walls of Lactobacillus plantarum N.I.R.D.C106Coapes, H. E. January 1970 (has links)
No description available.
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Some aspects of cytosine and dihydrocytosine chemistryCoe, Paul Frederick January 1970 (has links)
No description available.
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Studies on the specific inactivation of pyridoxal phosphate enzymesCharteris, Alan Thomas January 1978 (has links)
No description available.
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Multiple forms of human ß-galactosidaseCheetham, P. S. J. January 1975 (has links)
No description available.
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The comparative metabolism and excretion of certain chlorinated cyclodiene insecticides in vertebrate speciesChipman, James Kevin January 1978 (has links)
No description available.
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Studies on a DNA polymerase from Physarum polycephalumChoudhry, M. K. January 1978 (has links)
No description available.
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Hydrophobic interactions and liquid crystallisation in collagen assemblyHu, Xiao Wen January 1996 (has links)
No description available.
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