Expanding global populations, unequal food distribution and disease pressure suggest food poverty is increasing. Consequently, much attention is focussed on alternative natural methods in which to increase agricultural yield. Previously, it was observed that Trichoderma hamatum strain GD12 and its respective N-acetyl-β-D-Glucosamine mutant ∆Thnag:hph promoted plant biomass and fitness that, as a result, may provide a credible natural alternative to synthetic fertilisers. However, on a molecular level, the manner in which this is achieved has not been fully elucidated. In this thesis, I report the biofertiliser effect of GD12 and mutant ∆Thnag::hph once applied to autoclaved peat microcosms as sole applications. Furthermore, I demonstrate the biocontrol ability of GD12 when co-inoculated with Sclerotinia sclerotiorum or Rhizoctonia solani and reveal, that once mycelium co-inoculation has occurred, GD12 increase plant biomass and provide protection; whilst ∆Thnag::hph does not. Consequently, I challenged the biocontrol effects of Trichoderma metabolite extract where I validate that both Trichoderma wild type GD12 and mutant ∆Thnag::hph are incapable of suppressing pathogen growth. Subsequently, I characterised the up-regulated signatures associated with GD12 and ∆Thnag::hph using LC-MS techniques where unique compounds were discovered from each strain of Trichoderma. In conclusion, I provide evidence that N-acetyl-β-D-Glucosamine mutation bring about metabolomic changes that affect the fungal secretome which, in turn, alters plant phenotype, fitness and germination. Furthermore, I have shown that these effects are species specific and depend upon pathogen, plant and fungal properties. However, further investigations are needed to fully elucidate the compound(s) responsible for biocontrol and biofertilisation; especially plant-specific effects that take place as a consequence of fungal activity.

Les transitions agroécologiques impliquent une transformation radicale des modes de production, mais également des modes de transformation, de distribution et de consommation, du conseil agricole, des politiques publiques et de la recherche - en d’autres termes : une reconfiguration du système agri-alimentaire. Dans la vallée de la Drôme, la forte proportion d’acteurs plaçant l’agriculture biologique au coeur de leur stratégie de développement et leur collaboration dans le projet Biovallée semblent indiquer qu’une transition agroécologique est en cours et que l’échelle territoriale offre des leviers permettant de la déclencher. Cette thèse propose une analyse historique des dynamiques à l’oeuvre sur ce territoire, afin de contribuer à la compréhension des mécanismes de transition et à leur conceptualisation. En mobilisant la théorie des transitions sociotechniques et à travers une approche pragmatique et ethnographique, nous montrons qu’une reconfiguration du système agri-alimentaire territorial est en cours, et qu’elle résulte des interactions entre une configuration sociotechnique dominante et deux configurations alternatives qu’ont construites les acteurs au fil du temps, autour de deux paradigmes alternatifs à la modernisation agricole. Nous montrons comment, à travers ces interactions, les acteurs parviennent à modifier les rapports de force régissant le système agri-alimentaire, et que l’échelle territoriale leur offre des marges de manoeuvre inexistantes à une échelle plus large. Nous mettons en évidence le rôle essentiel d’une diversité d’initiatives, au sein desquelles les acteurs ont progressivement construit de nouvelles formes de coordination. Dès lors, la question est non plus de penser les transitions à partir du développement d’une innovation technique, mais de créer les conditions pour favoriser la coexistence d’une diversité d’initiatives, porteuses d’innovations sociales, et leurs interactions avec le système dominant. / Agriculture’s transition towards agrocology requires a radical transformation of production practices based on ecological principles, but it also requires radical changes within transformation, distribution and consumption practices and within advisory systems, public policies and research. In other words, it requires a profound reconfiguration of the whole agrifood system. In the Drome Valley (France), the high proportion of actors who consider organic agriculture as central in their development strategy and their involvement within the “Biovallée” project, suggest that an agroecological transition is in process and that the territorial scale might facilitate it. This thesis analyses the agricultural dynamics at the scale of this territory since the 1970s, in order to understand the transition mechanisms and to conceptualize them. Based on a framework inspired from the sustainable transition theories and on an ethnographic and pragmatic approach, it shows that a reconfiguration of the whole local agrifood system is indeed in process, and that it results from the interactions between a dominant sociotechnical configuration and two alternative ones which local actors have set up over time around two alternative paradigms. We analyze how actors succeed in changing the balance of power within the local agri-food system and how the territorial scale offers them some levers which do not exist on a larger scale. In this case, certain traditional actors and a diversity of initiatives allowed actors to progressively set up new forms of coordination, that is social or organizational innovations (rather than technological ones). Therefore the main issue shifts from thinking transitions based on the development of atechnological innovation, towards creating conditions in order to favour the coexistence of a diversity of initiatives that develop social innovations and to favour their interactions with the dominant system.

Thesis (MSc .(Plant Production)) -- University of Limpopo, 2016 / Delayed fruit harvest (DFH) or on-tree-fruit storage is a strategy being considered to extend the harvest season of late maturing ‘Reed’ avocado. However, avocado fruit growth beyond physiological maturity is accompanied by alterations in chemical and physical properties. Thus, the study aimed to investigate the effects of delayed harvest on tree productivity, mineral nutrition and post-harvest quality of late maturing ‘Reed’ avocado and further to evaluate the fruit quality response to varying ripening temperature regimes. As treatments, fruit were harvested early, mid and late in the season, with one month apart from each harvest during 2013 and 2014 seasons. Yield and fruit size were recorded at harvest. During each harvest time, two sets of fruit samples were collected to determine the fruit nutritional content while the second set was cold stored at 5.5°C for 28 days. After storage, fruit were ripened at 16, 21 or 25°C and evaluated for post-harvest disorders, pathological diseases and fruit physico-chemical properties. Yield dropped from 42 to 12 kg/tree from early to late harvest during 2013, while treatments showed no effect during 2014. Furthermore, treatments had no effect on fruit size during both seasons. Phosphorus content decreased by 40 and 23% from early to late harvest time during 2013 and 2014 seasons, respectively. Similar decreasing patterns were observed for K, Ca and Mg content. Zinc content remained constant during 2013; however, mid-season fruit maintained higher zinc levels during 2014 season when compared to early and late harvest time. Protein content was constant for early and mid-season fruit but significantly decreased during the late harvest in both seasons. Moisture content decreased from 74.0 to 65.0% from early to late harvest during both seasons. Starch, oil and ash content were high during late harvest time in both seasons; while crude fibre was high during the mid-harvest time when compared with early and late harvest time. Internal chilling injury increased with maturity and ripening temperature. In both seasons, higher temperatures induced higher anthracnose incidences during early and mid-harvest compared with late harvest time. Similarly, vascular browning was high on fruit ripened at 16°C during early and mid-harvest time but decreased on late harvest fruit during 2013. However, low ripening temperatures induced high incidences of vascular browning during 2014 season. Stem-end rot was high on fruit ripened at 16°C than 21 and 25°C during 2014 season. xvi Physico-chemical quality parameters showed similar behaviour in both seasons. Fruit lightness increased with ripening time regardless of ripening temperature during the early harvest, but remained constant during mid and late harvest. Hue angle (h0) and Chroma (C*) values were slightly reduced during ripening. Fruit ripened at 25°C had the highest respiration rate and reached a climacteric peak earlier (day 2) than fruit ripened at 21 and 16°C (day 4). Fruit firmness and weight loss were high and rapid at 25°C followed by 21 and 16°C regardless of the harvest time. Delayed harvest had no effect on yield and fruit size. Generally, nutritional content of avocado fruit increased with fruit maturity. Furthermore, fruit maturity played a major role in the response of fruit to ripening temperature. Higher temperature enhanced fruit ripening, but was conducive for development of post-harvest diseases. Keywords: Harvesting time, nutrition, yield, fruit size, ripening temperature, post-harvest quality