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Studium interakce adsorbátu s pasivovanými povrchy Si pomocí STM / Studium interakce adsorbátu s pasivovanými povrchy Si pomocí STMMatvija, Peter January 2013 (has links)
The scanning tunneling microscopy is used to study the morphology of Tl adlayer in various stages of Tl desorption from the Si(111) surface and to study behaviour of various adsorbates on the Si(111)/Tl-(1 × 1). The utilization of thallium layer for passivation of the Si(111) was examined closely for various adsorbates. Manganese, aluminium, indium and tin layers which were directly deposited onto the Si(111)-(7 × 7) were compared with the layers prepared by deposition of adsorbate onto the passivating layer after the subsequent thermal desorption of Tl (after annealing at ≈ 400◦ C). Examined adsorbates exhibited signs of extremely high diffusivity and weak bond with the surface Si(111)/Tl- (1 × 1). The passivating layer was stable against the adsorbates.The application of thallium in the role of surfactant caused lowering of temperature and coverage needed for the preparation of reconstructions which were observed on the surfaces prepared by the direct deposition of adsorbate. 1
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89Zr-Imuno-PET/111In-Imuno- SPECT: desenvolvimento radiofarmacêutico de agentes de imagem molecular para receptores EGF / 89Zr immuno-PET/111In imuno-SPECT: Radiopharmaceutical development of molecular imaging agents for EGF receptorsRaquel Benedetto 08 December 2017 (has links)
A baixa seletividade dos métodos convencionais para diagnóstico e terapia de neoplasias, bem como o fato de nem sempre alcançarem o sucesso terapêutico desejado, configuram dificuldades para a prática oncológica. Diante disso, os anticorpos monoclonais (AcMs) radiomarcados, aplicados em técnicas diagnósticas, têm se destacado, visto que permitem a entrega seletiva da radiação ao alvo de interesse. A metodologia Radioimunodiagnóstico (RID), utilizando AcM anti-EGFR radiomarcado, possibilita triagem prévia, avaliando a resistência ao tratamento e estratificando pacientes que possam apresentar benefícios à imunoterapia com cetuximabe. Além disso, permite monitorar a progressão da terapia, visando tratamento mais efetivo e direcionado, promulgando a abordagem da medicina personalizada. No Brasil, ainda não há radioimunoconjugado disponível para diagnóstico e seguimento do câncer. Nesse contexto, o objetivo com este trabalho foi o de desenvolver uma formulação farmacêutica para padronizar uma rotina de produção dos radiofármacos para radioimunodiagnóstico de câncer de cabeça e pescoço e de câncer colorretal: cetuximabe-DTPA-111In e cetuximabe-DFO-89Zr. Em adição, corroborar na elucidação dos mecanismos de resistência das células tumorais à terapia com o cetuximabe, através da realização de estudos de ligação do radioimunoconjugado à receptores celulares. Em relação aos radiofármacos estudados, destaca-se que os processos de conjugação do cetuximabe com os quelantes DTPA, na razão molar 1:20, e com o DFO, 1:5, foram bem-sucedidos e otimizados, demonstrando boa reprodutibilidade. Os imunoconjugados apresentaram preservação da imunorreatividade e alta estabilidade quando armazenados a -20°C por até seis meses. Esses imunoconjugados, quando radiomarcado com 111In e 89Zr, exibiram pureza radioquímica superior a 95%, sem necessidade de purificação pós-marcação, e estabilidade por tempo que possibilita seu transporte às clínicas distantes do centro produtor. As análises in vitro do cetuximabe-DTPA-111In em células FaDu-C10 (linhagem resistente) demonstraram percentual inexpressivo de ligação e internalização do radioimunoconjugado, congruindo na explanação do modelo de resistência conferido à linhagem. O estudo de corpo inteiro em MicroPET/TC revelou redução no perfil de captação no grupo de bloqueio, com excesso de cetuximabe não marcado, e intensa captação do cetuximabe-DFO-89Zr pelo tumor de células escamosas no grupo sem bloqueador, confirmando a especificidade in vivo do radioimunoconjugado. Os estudos de biodistribuição dos radiofármacos foram compatíveis com os descritos em literatura e validaram os resultados obtidos por imagens em MicroSPECT/TC e MicroPET/TC, além de apresentarem apreciável captação tumoral, considerando os tempos analisados. A estabilidade alta in vivo e a eficácia da marcação foram confirmadas pela baixa captação óssea e em tecidos não alvos. O melhor intervalo pós-injeção do radiofármaco para avaliação in vivo foi após cinco dias da administração. Conclui-se, portanto, que os radioimunoconjugados para imuno-SPECT e imuno-PET, cetuximabe-DTPA-111In e cetuximabe-DFO-89Zr, são ferramentas promissoras para diagnóstico e monitoramento de câncer receptor específico (EGFR) e para estratificação de pacientes à terapia anti-EGFR, encorajando a continuidade deste projeto para futuros estudos clínicos. / The low selectivity of conventional methods for cancer diagnosis and therapy, as well as the fact that these methods could not achieve the desired therapeutic success, constitute difficulties for the oncological practice. In this regard, radiolabeled monoclonal antibodies (mAbs) applied in diagnostic techniques have been highlighted, since they allow the selective delivery of the radiation to the specific target. The radioimmunodiagnosis methodology (RID), using radiolabeled anti-EGFR mAbs, enables previous screening, evaluating resistance to treatment and stratifying patients who may present benefits to cetuximab immunotherapy. In addition, it allows monitoring the progression of the therapy, aiming for a more effective and directed treatment, leading the personalized medicine approach. A radioimmunoconjugate is not yet available for diagnosis and management of cancer in Brazil. In this context, this research was carried out to develop a pharmaceutical formulation to standardize a routine production of radiopharmaceuticals for diagnosis and monitoring head and neck cancer and colorectal carcinoma: 111In-DTPA-cetuximab and 89Zr-DFO-cetuximab. In addition, corroborate in the elucidation of the tumor cells resistance mechanisms to EGFR-targeted therapy, through in vitro and in vivo radioimmunoconjugate binding studies to cellular receptors. Regarding to the radiopharmaceuticals studied, cetuximab was conjugated to DTPA chelator at 1:20 molar ratio and to DFO at 1: 5, and these processes were successful and optimized, showing good reproducibility. Immunoconjugates showed preservation of immunoreactivity and high stability when stored at -20 °C for up to 6 months. These immunoconjugates when radiolabeled with 111In and 89Zr have exhibited radiochemical purity above 95%, without any post-labeling purification, and the radioimmunoconjugates have demonstrated stability for a time that allows them to be transported to clinics far from the producer center. 111In-DTPA-cetuximab in vitro analyzes in FaDu-C10 cells (resistant cell line) has presented an inexpressive percentage of binding and internalization of the radioimmunoconjugate, ensuring the resistance model conferred to this cell line. The MicroPET/CT imaging study has revealed a reduction in uptake profile for \"Blocking\" group, with an excess of unlabeling cetuximab, and an intense 89Zr-DFO-cetuximab uptake in squamous cell tumor for \"Non-blocking\" group, that evidenced the in vivo radioimmunoconjugate specificity. The biodistribution studies of the radiopharmaceuticals were well-matched with those described in the literature and they validated the results obtained through the MicroSPECT/CT and MicroPET/ CT images. In addition, these studies in vivo have displayed a substantial tumor uptake, according with the analyzed time points. The radioimmunoconjugate showed high in vivo stability and labeling procedures efficiency, which were confirmed by low bone and non-target tissues uptake. The best post-injection interval for in vivo evaluation is after 5 days of radioimmunoconjugate administration. In conclusion, the radioimmunoconjugates for immuno-SPECT and immuno-PET, 111In-DTPA-cetuximab and 89Zr-DFO-cetuximab, are promising tools for diagnosis and monitoring of specific receptor cancer (EGFR), as well as for stratification of patients to anti-EGFR therapy, and thus encourages the continuity of this project for future clinical trials.
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Estudo da marcação com Indio-111 e Gálio-68 de derivados da bombesina e avaliação das propriedades biológicas para aplicação em SPECT e PET / Study of labelling of bombesin derivatives with 111-indium and 68-galium and evaluation of biological properties for application in SPECT and PETOliveira, Ricardo de Souza 10 December 2014 (has links)
Receptores para o peptídeo liberador de gastrina (GRPr) são super expressos em vários tipos de células cancerígenas, incluindo câncer de mama e próstata. A Bombesina é um análogo do peptídeo GRP de mamíferos que se liga com alta especificidade e afinidade aos receptores do peptídeo liberador de gastrina (GRPr). Significantes pesquisas têm sido realizadas para desenvolver e radiomarcar um análogo da bombesina para diagnóstico de tumores de próstata e mama, utilizando-se técnicas de SPECT e PET, com radionuclídeos como 111In e 68Ga. O objetivo deste trabalho foi estudar a marcação com 111In de uma série inédita de peptídeos derivados de bombesina e determinar o potencial de aplicação no diagnóstico de tumores de próstata em modelos animais. Vários estudos foram realizados para padronizar o procedimento de marcação, variando-se temperatura, tempo de marcação, massa do peptídeo e atividade do radionuclídeo. Os resultados demonstraram que os análogos da bombesina estudados podem ser marcados com índio-111 com alto rendimento de marcação e alta atividade específica. Os estudos de biodistribuição em animais normais demonstraram que o derivado de BBN com espaçador aminoacídico Gly5 apresentou captação pancreática e intestinal expressiva, sugerindo ser o melhor derivado. Dois derivados DOTA- conjugados, com espaçador Gly5 foram radiomarcados com gálio-68 e investigados em modelo animal com tumor de próstata humano, indicando o potencial para aplicação do peptídeo radiomarcado no diagnóstico por PET. / Gastrin releasing peptide receptors (GRPRs) are over expressed in various types of cancer cells including prostate and breast cancer. Bombesin is an analogue of the mammalian GRP that binds with high specificity and affinity to GRPRs. Significant research efforts have been devoted to the design and radiolabel bombesin peptides for the diagnostic of prostatic and breast cancer using SPECT e PET, with radionuclides like 111In e 68Ga. The objective of this work was to study the labeling with 111In of a new bombesin series and evaluate the potential for diagnostic application using animal model. Several studies were evaluated to optimize the labelling conditions of BBN derivatives with 111In using different temperature, time, mass of peptide and radionuclide activity. High radiochemical purity and high specific activity were obtained for all the peptides labeled with 111-indium. Biodistribution studies in normal mices showed that the BBN derivative with Gly5 as aminoacidic spacer presented high uptake on pancreas and intestines that suggests that is the best peptide. Two DOTA-derivatives with Gly5 as spacer were radiolabelled with 68-gallium and evaluated in tumor model animals of human prostatic cancer and showed high potential for clinical application in diagnostic procedures with PET.
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\"Estudo da reação de eletro-oxidação de metanol sobre eletrodos monocristalinos de platina modificados com eletrodepósitos de cobre e estanho\" / \"Study of the electrooxidation of methanol on platinum single-crystal electrodes modified with copper and tin electrodeposits\"Paulino, Marcia Elizangela 26 October 2006 (has links)
A eletro-oxidação de metanol foi estudada em eletrodos de platina monocristalina de índices de Miller (100) e (111) modificados superficialmente com metais cobre e estanho nas condições de UPD (Underpotential Deposition) e OPD (Overpotential Deposition). Os experimentos foram conduzidos em EDRMP (Eletrodo de Disco Rotatório com Menisco Pendente) em conjunto com as técnicas eletroquímicas. Os depósitos de cobre foram realizados em condições de UPD e OPD enquanto que estanho em OPD, dada as limitações de rearranjo estrutural da superfície cristalográfica do eletrodo. As análises dos resultados indicaram que o grau de recobrimento dos metais sobre o suporte é menor que uma monocamada, cerca de 10% desta. Tais resultados comparados com a literatura indicam a formação de aglomerados, ilhas, em estruturas bi e tridimensionais no caso do cobre. Em estanho foi atingido maior grau de recobrimento. No entanto, comparando os resultados para estanho com o da literatura, este não se depositou na forma de adátomos, mas como hidróxido ou sulfato. Esta forma de interação do estanho com a platina é a responsável pela diminuição do potencial de oxidação do metanol sobre o substrato. Na eletro-oxidação do metanol os melhores resultados foram obtidos na presença de estanho, no entanto ressalta-se que neste caso a influência da rotação não foi significante e as medidas de cronoamperometria indicaram melhor atividade nesta situação com o eletrodo modificado. / Methanol oxidation reaction was studied with copper and tin modified single-crystal electrodes. This study was carried out using UPD and OPD techniques using the EDRMP configurations the results showed that the coverage degree of copper and tin on platinum single-crystal as less than one monolayer (_ 10% monolayer) and possible with the formation of islands in bi and tridimenision. The deposition of copper was not good to the methanol oxidation reaction, possible due to a higher solvatation by anions at the Cu sites, conducting to a blocking effect on the Pt neighboring atoms. The tin deposition have shown a higher activity for methanol oxidation reaction. This ascribed to the hydroxides formation at tin sites, which increase the kinetics of CO oxidation following the bifunctional mechanism.
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89Zr-Imuno-PET/111In-Imuno- SPECT: desenvolvimento radiofarmacêutico de agentes de imagem molecular para receptores EGF / 89Zr immuno-PET/111In imuno-SPECT: Radiopharmaceutical development of molecular imaging agents for EGF receptorsBenedetto, Raquel 08 December 2017 (has links)
A baixa seletividade dos métodos convencionais para diagnóstico e terapia de neoplasias, bem como o fato de nem sempre alcançarem o sucesso terapêutico desejado, configuram dificuldades para a prática oncológica. Diante disso, os anticorpos monoclonais (AcMs) radiomarcados, aplicados em técnicas diagnósticas, têm se destacado, visto que permitem a entrega seletiva da radiação ao alvo de interesse. A metodologia Radioimunodiagnóstico (RID), utilizando AcM anti-EGFR radiomarcado, possibilita triagem prévia, avaliando a resistência ao tratamento e estratificando pacientes que possam apresentar benefícios à imunoterapia com cetuximabe. Além disso, permite monitorar a progressão da terapia, visando tratamento mais efetivo e direcionado, promulgando a abordagem da medicina personalizada. No Brasil, ainda não há radioimunoconjugado disponível para diagnóstico e seguimento do câncer. Nesse contexto, o objetivo com este trabalho foi o de desenvolver uma formulação farmacêutica para padronizar uma rotina de produção dos radiofármacos para radioimunodiagnóstico de câncer de cabeça e pescoço e de câncer colorretal: cetuximabe-DTPA-111In e cetuximabe-DFO-89Zr. Em adição, corroborar na elucidação dos mecanismos de resistência das células tumorais à terapia com o cetuximabe, através da realização de estudos de ligação do radioimunoconjugado à receptores celulares. Em relação aos radiofármacos estudados, destaca-se que os processos de conjugação do cetuximabe com os quelantes DTPA, na razão molar 1:20, e com o DFO, 1:5, foram bem-sucedidos e otimizados, demonstrando boa reprodutibilidade. Os imunoconjugados apresentaram preservação da imunorreatividade e alta estabilidade quando armazenados a -20°C por até seis meses. Esses imunoconjugados, quando radiomarcado com 111In e 89Zr, exibiram pureza radioquímica superior a 95%, sem necessidade de purificação pós-marcação, e estabilidade por tempo que possibilita seu transporte às clínicas distantes do centro produtor. As análises in vitro do cetuximabe-DTPA-111In em células FaDu-C10 (linhagem resistente) demonstraram percentual inexpressivo de ligação e internalização do radioimunoconjugado, congruindo na explanação do modelo de resistência conferido à linhagem. O estudo de corpo inteiro em MicroPET/TC revelou redução no perfil de captação no grupo de bloqueio, com excesso de cetuximabe não marcado, e intensa captação do cetuximabe-DFO-89Zr pelo tumor de células escamosas no grupo sem bloqueador, confirmando a especificidade in vivo do radioimunoconjugado. Os estudos de biodistribuição dos radiofármacos foram compatíveis com os descritos em literatura e validaram os resultados obtidos por imagens em MicroSPECT/TC e MicroPET/TC, além de apresentarem apreciável captação tumoral, considerando os tempos analisados. A estabilidade alta in vivo e a eficácia da marcação foram confirmadas pela baixa captação óssea e em tecidos não alvos. O melhor intervalo pós-injeção do radiofármaco para avaliação in vivo foi após cinco dias da administração. Conclui-se, portanto, que os radioimunoconjugados para imuno-SPECT e imuno-PET, cetuximabe-DTPA-111In e cetuximabe-DFO-89Zr, são ferramentas promissoras para diagnóstico e monitoramento de câncer receptor específico (EGFR) e para estratificação de pacientes à terapia anti-EGFR, encorajando a continuidade deste projeto para futuros estudos clínicos. / The low selectivity of conventional methods for cancer diagnosis and therapy, as well as the fact that these methods could not achieve the desired therapeutic success, constitute difficulties for the oncological practice. In this regard, radiolabeled monoclonal antibodies (mAbs) applied in diagnostic techniques have been highlighted, since they allow the selective delivery of the radiation to the specific target. The radioimmunodiagnosis methodology (RID), using radiolabeled anti-EGFR mAbs, enables previous screening, evaluating resistance to treatment and stratifying patients who may present benefits to cetuximab immunotherapy. In addition, it allows monitoring the progression of the therapy, aiming for a more effective and directed treatment, leading the personalized medicine approach. A radioimmunoconjugate is not yet available for diagnosis and management of cancer in Brazil. In this context, this research was carried out to develop a pharmaceutical formulation to standardize a routine production of radiopharmaceuticals for diagnosis and monitoring head and neck cancer and colorectal carcinoma: 111In-DTPA-cetuximab and 89Zr-DFO-cetuximab. In addition, corroborate in the elucidation of the tumor cells resistance mechanisms to EGFR-targeted therapy, through in vitro and in vivo radioimmunoconjugate binding studies to cellular receptors. Regarding to the radiopharmaceuticals studied, cetuximab was conjugated to DTPA chelator at 1:20 molar ratio and to DFO at 1: 5, and these processes were successful and optimized, showing good reproducibility. Immunoconjugates showed preservation of immunoreactivity and high stability when stored at -20 °C for up to 6 months. These immunoconjugates when radiolabeled with 111In and 89Zr have exhibited radiochemical purity above 95%, without any post-labeling purification, and the radioimmunoconjugates have demonstrated stability for a time that allows them to be transported to clinics far from the producer center. 111In-DTPA-cetuximab in vitro analyzes in FaDu-C10 cells (resistant cell line) has presented an inexpressive percentage of binding and internalization of the radioimmunoconjugate, ensuring the resistance model conferred to this cell line. The MicroPET/CT imaging study has revealed a reduction in uptake profile for \"Blocking\" group, with an excess of unlabeling cetuximab, and an intense 89Zr-DFO-cetuximab uptake in squamous cell tumor for \"Non-blocking\" group, that evidenced the in vivo radioimmunoconjugate specificity. The biodistribution studies of the radiopharmaceuticals were well-matched with those described in the literature and they validated the results obtained through the MicroSPECT/CT and MicroPET/ CT images. In addition, these studies in vivo have displayed a substantial tumor uptake, according with the analyzed time points. The radioimmunoconjugate showed high in vivo stability and labeling procedures efficiency, which were confirmed by low bone and non-target tissues uptake. The best post-injection interval for in vivo evaluation is after 5 days of radioimmunoconjugate administration. In conclusion, the radioimmunoconjugates for immuno-SPECT and immuno-PET, 111In-DTPA-cetuximab and 89Zr-DFO-cetuximab, are promising tools for diagnosis and monitoring of specific receptor cancer (EGFR), as well as for stratification of patients to anti-EGFR therapy, and thus encourages the continuity of this project for future clinical trials.
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Electrodeposition of ultrathin Pd, Co and Bi films on well-defined noble-metal electrodes: studies by ultrahigh vacuum-electrochemistry (UHV-EC)Baricuatro, Jack Hess L 30 October 2006 (has links)
Three illustrative cases involving the electrodeposition of ultrathin metal
films of varying reactivities onto noble-metal substrates were investigated: (i) Pd
on Pt(111), a noble admetal on a noble-metal surface; (ii) Bi on Pd(111), a less
noble admetal on a noble-metal surface; and (iii) Co on polycrystalline Pd and
Pd(111), a reactive metal on a noble-metal surface. The interfacial
electrochemistry of these prototypical systems was characterized using a
combination of electrochemical methods (voltammetry and coulometry) and
ultrahigh vacuum electron spectroscopies (Auger electron spectroscopy, AES;
low energy electron diffraction, LEED; and X-ray photoelectron spectroscopy,
XPS).
Potential-controlled adsorption-desorption cycles of aqueous bromide
exerted surface smoothening effects on ultrathin Pd films with defect sites
(steps). This procedure, dubbed as electrochemical (EC) annealing, constituted a
nonthermal analogue to conventional annealing. EC-annealed ultrathin Pd films
exhibited long-range surface order and remained free of oxygen adspecies. Pdadatoms occupying step-sites were selectively dissolved and/or rearranged to
assume equilibrium positions in a well-ordered (1x1) film.
Electrodeposition of Co was found to be highly surface-structuresensitive.
While virtually no Co electrodeposition transpired on a clean Pd(111)
surface, Co was voltammetrically deposited on (i) a Pd(111) electrode
roughened by oxidation-reduction cycles; and (ii) thermally annealed
polycrystalline Pd, which is a composite of the (111) and (100) facets.
Electrodeposition of Co was also observed to be kinetically hindered and slow
potential scan rates (0.1 mV/s) were required.
Well-defined ultrathin Bi films were potentiostatically electrodeposited
onto Pd(111); a Stranski-Krastanov growth mode was indicated. The
electrochemical reactivity of ultrathin Bi films was characterized using two
surface probes: aqueous iodide and D-glucose. (i) Exposure of the prepared Bi
adlayers (ÃÂBi 0.33) to aqueous iodide gave rise to (âÂÂ3xâÂÂ7) I-on-Bi superlattice.
The same superlattice was obtained if Bi was electrodeposited onto
Pd(111)(âÂÂ3xâÂÂ3)R30o-I. (ii) With respect to electrooxidation of D-glucose on
Pd(111), the presence of Bi adlayers inhibited the by-product-induced "surface
poisoning" of Pd(111) but reduced its electrocatalytic efficiency.
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Estudo da marcação com Indio-111 e Gálio-68 de derivados da bombesina e avaliação das propriedades biológicas para aplicação em SPECT e PET / Study of labelling of bombesin derivatives with 111-indium and 68-galium and evaluation of biological properties for application in SPECT and PETRicardo de Souza Oliveira 10 December 2014 (has links)
Receptores para o peptídeo liberador de gastrina (GRPr) são super expressos em vários tipos de células cancerígenas, incluindo câncer de mama e próstata. A Bombesina é um análogo do peptídeo GRP de mamíferos que se liga com alta especificidade e afinidade aos receptores do peptídeo liberador de gastrina (GRPr). Significantes pesquisas têm sido realizadas para desenvolver e radiomarcar um análogo da bombesina para diagnóstico de tumores de próstata e mama, utilizando-se técnicas de SPECT e PET, com radionuclídeos como 111In e 68Ga. O objetivo deste trabalho foi estudar a marcação com 111In de uma série inédita de peptídeos derivados de bombesina e determinar o potencial de aplicação no diagnóstico de tumores de próstata em modelos animais. Vários estudos foram realizados para padronizar o procedimento de marcação, variando-se temperatura, tempo de marcação, massa do peptídeo e atividade do radionuclídeo. Os resultados demonstraram que os análogos da bombesina estudados podem ser marcados com índio-111 com alto rendimento de marcação e alta atividade específica. Os estudos de biodistribuição em animais normais demonstraram que o derivado de BBN com espaçador aminoacídico Gly5 apresentou captação pancreática e intestinal expressiva, sugerindo ser o melhor derivado. Dois derivados DOTA- conjugados, com espaçador Gly5 foram radiomarcados com gálio-68 e investigados em modelo animal com tumor de próstata humano, indicando o potencial para aplicação do peptídeo radiomarcado no diagnóstico por PET. / Gastrin releasing peptide receptors (GRPRs) are over expressed in various types of cancer cells including prostate and breast cancer. Bombesin is an analogue of the mammalian GRP that binds with high specificity and affinity to GRPRs. Significant research efforts have been devoted to the design and radiolabel bombesin peptides for the diagnostic of prostatic and breast cancer using SPECT e PET, with radionuclides like 111In e 68Ga. The objective of this work was to study the labeling with 111In of a new bombesin series and evaluate the potential for diagnostic application using animal model. Several studies were evaluated to optimize the labelling conditions of BBN derivatives with 111In using different temperature, time, mass of peptide and radionuclide activity. High radiochemical purity and high specific activity were obtained for all the peptides labeled with 111-indium. Biodistribution studies in normal mices showed that the BBN derivative with Gly5 as aminoacidic spacer presented high uptake on pancreas and intestines that suggests that is the best peptide. Two DOTA-derivatives with Gly5 as spacer were radiolabelled with 68-gallium and evaluated in tumor model animals of human prostatic cancer and showed high potential for clinical application in diagnostic procedures with PET.
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\"Estudo da reação de eletro-oxidação de metanol sobre eletrodos monocristalinos de platina modificados com eletrodepósitos de cobre e estanho\" / \"Study of the electrooxidation of methanol on platinum single-crystal electrodes modified with copper and tin electrodeposits\"Marcia Elizangela Paulino 26 October 2006 (has links)
A eletro-oxidação de metanol foi estudada em eletrodos de platina monocristalina de índices de Miller (100) e (111) modificados superficialmente com metais cobre e estanho nas condições de UPD (Underpotential Deposition) e OPD (Overpotential Deposition). Os experimentos foram conduzidos em EDRMP (Eletrodo de Disco Rotatório com Menisco Pendente) em conjunto com as técnicas eletroquímicas. Os depósitos de cobre foram realizados em condições de UPD e OPD enquanto que estanho em OPD, dada as limitações de rearranjo estrutural da superfície cristalográfica do eletrodo. As análises dos resultados indicaram que o grau de recobrimento dos metais sobre o suporte é menor que uma monocamada, cerca de 10% desta. Tais resultados comparados com a literatura indicam a formação de aglomerados, ilhas, em estruturas bi e tridimensionais no caso do cobre. Em estanho foi atingido maior grau de recobrimento. No entanto, comparando os resultados para estanho com o da literatura, este não se depositou na forma de adátomos, mas como hidróxido ou sulfato. Esta forma de interação do estanho com a platina é a responsável pela diminuição do potencial de oxidação do metanol sobre o substrato. Na eletro-oxidação do metanol os melhores resultados foram obtidos na presença de estanho, no entanto ressalta-se que neste caso a influência da rotação não foi significante e as medidas de cronoamperometria indicaram melhor atividade nesta situação com o eletrodo modificado. / Methanol oxidation reaction was studied with copper and tin modified single-crystal electrodes. This study was carried out using UPD and OPD techniques using the EDRMP configurations the results showed that the coverage degree of copper and tin on platinum single-crystal as less than one monolayer (_ 10% monolayer) and possible with the formation of islands in bi and tridimenision. The deposition of copper was not good to the methanol oxidation reaction, possible due to a higher solvatation by anions at the Cu sites, conducting to a blocking effect on the Pt neighboring atoms. The tin deposition have shown a higher activity for methanol oxidation reaction. This ascribed to the hydroxides formation at tin sites, which increase the kinetics of CO oxidation following the bifunctional mechanism.
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'Boundary' : an expression of the dynamic unity between man and environment : building a paradigm to unravel the mind's fundamental kinship with the cosmos and its role as the vehicle of the universe's unfolding meaningSaridaki, Maria January 2012 (has links)
The aim of this thesis is to build a paradigm to unravel the human mind’s fundamental kinship with the cosmos and its role as the vehicle of the universe’s unfolded meaning. The concept of ‘boundary’ is presented as a primary ontological force that drives, provokes and defines our thinking, consciously and subconsciously, in our attempt to achieve an understanding of self within the cosmos. It provides the hidden thread, the ‘limited concept’ that acts as a guide towards the building of this paradigm. Challenging its primarily physical interpretation, this thesis examines the concept of boundary from its genesis, imbedded in the primary moment of the birth of human consciousness within the universe, following it along its progressive complexity. Merging a primarily phenomenological with an epistemological approach by building on a number of essential evolutionary phases in our existence, through a synthesis of induction and deduction, we are confronted by how they are driven by boundary. Myth, religion, language, culture, philosophy, science, and even architecture are manifestations of humanity’s gradual attempt to understand, adapt to and transform our world and ourselves within it and in reference to it, displaying an inherent dynamic between our mind and our world. Bound in this dialectical creative opposition, our conceptualisations of the world are seen both as insights of our mind in its attempt to unravel the meaning of the cosmos, as well as the cosmos’s attempt to gradually reveal its nature within us, thus revealing their radical kinship. Ultimately, the aim is to reveal architecture and its embodied nature as a fundamental manifestation of our existence within the cosmos and to distil its message and purpose, its timeless task. Architecture is exposed as an existential medium, engaging the boundary between man and the cosmos, inviting us to read a model of the world while at the same time endowing us with our own sense of self and finally enabling us to coexist with our world in an interactive evolving equilibrium.
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Time, change and reality : a new theory of persistencePickup, Martin James January 2012 (has links)
In my thesis I will be proposing situationalism: a new theory of how it is that things change over time. It is B-theoretic, eternalist and endurantist. The central contention of the theory is that what is true can differ in a metaphysically significant way from time to time. The theory emerges as a solution to the problem of change. In my first chapter, I argue that change is genuinely problematic (contra some of the recent literature). There are at least three ways to generate problems from change, and I elucidate problems from the law of non-contradiction and the indiscernibility of identicals. In the second chapter, I examine the nature of change and contend that the current major solutions to the problem fail to uphold our intuitive notion of change. Chapter 3 introduces the idea of a situation; a part of reality. The fourth chapter applies situations to the problem of change and comes up with a new solution. The solution relies on a denial of universal persistence; the denial of the idea that what is true in a situation s is thereby true in every situation of which s is a part. Chapter 5 considers the infamous Ship of Theseus case, and concludes that situationalism can also solve this problem. The situationalist account of the Ship of Theseus puzzle enables us, in Chapter 6, to briefly demonstrate the analogous application of the solution to a series of other persistence puzzles. The seventh chapter discusses the metaphysical consequences of such a view. The core claim is of the primacy of parts of reality over reality as a whole. It is a position according to which truth in situations is fundamental and situations needn’t cohere. I hold that the theory has significant costs but also substantial benefits. For this reason it is worth serious consideration.
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