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Modulation of ultrviolet light induced skin carcinogenesis by extracts of Rooibos and Honeybush using a mouse model:elucidating possible protective mechanismsPetrova, Antoinette January 2009 (has links)
Thesis(M Tech(Biomedical Technology))--Cape Peninsula University of Technology, 2009 / This thesis provides the first scientific evidence of the photoprotective properties of rooibos
and honeybush herbal tea extracts and to some extent, two major honeybush polyphenols,
hesperidin and mangiferin. These properties were demonstrated using in vivo models by:
Providing evidence for the inhibition of tumour promotion by ultraviolet B (UVB)
radiation in a two-stage skin carcinogenesis mouse model. Topical application of
polyphenol-rich extracts of rooibos and honeybush prior to UVB tumour promotion of
7,12-dimethylbenz[a]anthracene (DMBA)-initiated mouse skin, inhibited the formation
of tumours. The rooibos and honeybush extracts decreased the incidence and volume
of the tumours. Topical application of hesperidin and mangiferin were less effective
than the honeybush extracts as only the tumour volume was decreased, but not the
incidence.
Providing evidence for the inhibition of photodamage of the skin by UVB exposure in a
mouse model. Topical application of polyphenolic rich extracts of honeybush prior to
UVB irradiation of mouse skin reduced erythema, peeling, oedema and hyperplasia.
The depletion of antioxidant enzymes catalase and superoxide dismutase (SOD) was
prevented. The extracts protected the skin from oxidative and direct DNA damage, and
reduced lipid peroxidation. The induction of cyclooxygenase-2 (COX-2) and ornithine
decarboxylase (ODC) was also reduced. Topical application of the polyphenols
hesperidin and mangiferin showed reduced protective effects compared to the extracts.
Suggesting the possible mechanisms by which honeybush and the polyphenols protect
against photocarcinogenesis such as reducing tumour promotion, inflammation and
oxidative stress.
Suggesting the benefits of including honeybush and rooibos as cosmeceuticals in skin
care products and sunscreens as part of the strategy for preventing skin cancer.
Discussing the recommendations for further study such as investigating more specific
chemopreventive activities of these two South African herbal teas and their
polyphenols, dose response studies and clinical evaluations.
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Downregulation of RalGTPase-activating protein promotes invasion of prostatic epithelial cells and progression from intraepithelial neoplasia to cancer during prostate carcinogenesis / RalGAPの発現低下は前立腺発癌過程において、前立腺上皮細胞の浸潤能を亢進し上皮内腫瘍から癌への進行を促進する。Uegaki, Masayuki 25 November 2019 (has links)
京都大学 / 0048 / 新制・課程博士 / 博士(医学) / 甲第22115号 / 医博第4528号 / 新制||医||1039(附属図書館) / 京都大学大学院医学研究科医学専攻 / (主査)教授 松田 道行, 教授 武藤 学, 教授 小川 誠司 / 学位規則第4条第1項該当 / Doctor of Medical Science / Kyoto University / DFAM
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A quantitative study of the morphology of normal, hyperplastic, and neoplastic mammary tissue in the mouseHill, Elizabeth J. 01 January 1979 (has links)
This study intends to provide a quantitative and qualitative histological account of two aspects of mammary gland morphology. First, the normal mouse during different functional phases including inactivity, pregnancy, lactation and involution is presented. Second, the abnormal changes of mammary hyperplasia and neoplasia - both spontaneous and exogenous - are examined.
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The role of Id-1 in prostate development and carcinogenesisLing, Ming-tat, Patrick., 凌明達. January 2003 (has links)
published_or_final_version / Anatomy / Doctoral / Doctor of Philosophy
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Functional studies of SEI-1 and eIF5A2: candidate oncogenes isolated from frequently amplified regions ofovarian carcinomasTang, Dongjiang., 唐東江. January 2006 (has links)
published_or_final_version / abstract / Clinical Oncology / Doctoral / Doctor of Philosophy
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Non-small cell lung cancer: from bench to bedsideHo, Chung-man., 何重文. January 2007 (has links)
published_or_final_version / abstract / Medicine / Master / Doctor of Medicine
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Molecular studies on endometrial and ovarian carcinogenesis陳君怡, Chan, Kwan-yi, Queeny. January 2007 (has links)
published_or_final_version / abstract / Pathology / Doctoral / Doctor of Philosophy
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Functional characterization of liver intestine-cadherin (CDH17) in hepatocellular carcinomaChan, Wai-man, Vivian, 陳慧雯 January 2006 (has links)
published_or_final_version / abstract / Surgery / Master / Master of Philosophy
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Role of dedicator of cytokinesis I (DOCK180) in ovarian cancerZhao, Fung, 趙楓 January 2010 (has links)
published_or_final_version / Pathology / Master / Master of Philosophy
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Ras oncogenes and p53 suppressor genes in fish carcinogenesis modelsCheng, Ronshan 08 August 1995 (has links)
A digoxigenin-labeled nonradioactive detection system was used
to screen a zebrafish cDNA library for p53-like and ras-like genes.
One clone was isolated and identified as an incomplete p53-like
gene. The insert size of this clone is 1777 bp, which encodes part of
evolutionarily conserved region II and all of regions III, IV, and V. A
magnetically enriched whole zebrafish cDNA library was constructed
to enhance possible recovery of ras-like genes in zebrafish. One
clone, termed Zras-Bl, carried an insert of 2592 bp with an open
reading frame encoding a 188 amino acid residue ras p21 protein.
Based on total protein sequence, this expressed zebrafish ras p21 is
most closely related to human N-ras (91% homology), with lesser
homology to Ha-ras (84%) and Ki-ras (85%). Preliminary partial
sequence data obtained by genomic and reverase transcriptasepolymerase
chain reaction (RT-PCR) screening indicate the presence
of at least one additional expressed ras gene in zebrafish.
The tumorigenicity and Ki-ras mutational properties of dietary
7,12-dimethylbenz[a]anthracene (DMBA) and dibenzo[a,l]pyrene (DBP) were compared in rainbow trout. Both chemicals elicited
predominantly 12(1)G->A and 12(2)G->T mutations in trout liver
tumors. Two {12(1)G->T and 12(2)G->T} and one {12(1)G->A and
12(2)G->T} double mutation were also observed in DBP livers
tumors, but not in DMBA liver tumors. Some stomach tumors from
both chemicals exhibited so much DNA degradation that routine PCR
amplification was not possible. Among sixteen DMBA stomach
tumors with intact DNA, no Ki-ras mutations were found. Of sixteen
DBP stomach tumors examined, one had 12(1)G->A and two had
13(1)G->C mutations. The observed G->T transversions are
compatible with apurinic mutagenesis driven by unstable DNA
adducts arising from one-electron oxidation, but this is not true for
the major G->A transitions or G->C transversions and rare double
mutations found in this study. The low sensitivity of direct
sequencing may limit the frequency of ras mutant detection in this
study. / Graduation date: 1996
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