The stress and fatigue analysis of roller chain under varied loading is investigated in this study. With the dynamic responses of a gear-cam intermittent mechanism, boundary conditions are set up to the finite element model. The commercial MARC finite element method package is used in this work. Stress distribution is our concern. ASME elliptic equation and AGMA allowable contact stress criteria are employed in the fatigue analysis. Results indicate that the maximum of von Mises stress occurs on the contact surface between pins and plates. Besides, the initial failures on this loading are predicted to happen on the plates according to the fatigue analysis.