Viral illnesses are the most common agents affecting humans. Due to their widespread affects, viruses may have a particular influence on exercise functional capacity. Therefore, the purpose of this study was to determine the impact of an Upper Respiratory Infection (URI) on exercise functional capacity, as measured by VO2max. In addition, submaximal exercise responses, changes in resting pulmonary function, the impact of select symptoms, and level of initial fitness on performance during an URI were also determined. Forty-five subjects (females=21, male=24) between 18 and 29 years of age participated in this study and were assigned to a mild or severe illness group based on symptom severity. There was also a control group of 10 subjects (female=5, male=5). An initial serological screening was performed on all experimental subjects to assess for the RV16 antibody.Subjects testing negative for the antibody performed a baseline graded exercise test to volitional fatigue, as well as pulmonary function tests. Each subject was inoculated two consecutive days with the RV16 virus within ten days of the baseline exercise test. The day following the second inoculation (peak illness) the subjects performed post-pulmonary function and exercise tests. The control subjects performed two resting pulmonary function and exercise tests separated by one week. Significance was set at p<0.05. Repeated measures ANOVA revealed a significant interaction in V02 at submaximal and maximal levels between trials for all groups (p<0.045). A significant interaction for VE for all levels and all groups was found (p<0.002). No impairment in resting pulmonary function was observed. Analyses of symptoms, and initial V02 in regard to performance, also revealed no significant differences. Therefore, the results seem to indicate that an URI does not limit one's ability to perform at submaximal or maximal levels of exercise, however, some relationship seems to exist. Further research is needed to clarify the effects of an URI on physical performance. / School of Physical Education

To investigate the effect of a 12-week internet-based PA intervention (Active Living Every Day (ALED)) on average steps/day and time spent in sedentary, light, and moderate/vigorous activity/day, PA was objectively measured at baseline and follow-up using the Omron pedometer and the Actigraph GT3X in 11 bariatric patients (2 male, 9 female; 41±12 years). Significant changes (p<.05) were found in weight, BMI, body fat %, waist and hip circumference, submaximal RPE, percentage of individuals reporting PA and perceived exercise barriers from baseline to follow-up. No significant differences were found in steps/day or daily activity level from baseline to follow-up. Bariatric participants averaged 4454±203 steps/day and 74% of their day was spent sedentary. Based on this data it appears that the 12 week PA intervention was not effective in significantly changing PA behavior in the pre-surgery, but lead to improvements in cardiovascular risk factors, RPE and potential exercise barriers. / School of Physical Education, Sport, and Exercise Science

The purpose of this research was to examine the effects of a premovement silent period (PSP), and an involuntary small amplitude countermovement (CM) on the maximal height of the squat jump (SJ). Fifteen elite male volleyball players (20.6 ± 1.6 yrs) and thirteen untrained males (20.2 ± 1.7 yrs) performed ten maximal effort SJs from identical starting positions. The electromyographic activity of the vastus lateralis and biceps femoris was measured in conjunction with the vertical ground reaction force, and the vertical displacement measured by a cable-extension transducer. The presence of a PSP or small amplitude CM did not increase maximal SJ height significantly (p > 0.05). These findings indicate that in the SJ, facilitation effects of the PSP and small amplitude CM are minimal. This is important to past and future studies utilizing the SJ, as it is often difficult for individuals to perform SJs with no detectable CM. / School of Physical Education

Despite similar rates of energy expenditure during training, competitive swimmers have been shown to store significantly greater amounts of body fat than competitive runners. In an attempt to explain these discrepancies, male collegiate swimmers (n=8) and runners (n=8) were monitored during 45 min of swimming and running, respectively (75% V02 max), and during two hours of recovery. In addition, a group of male competitive triathletes (n=6) were similarly monitored during and after both swimming and running exercise.Blood samples were obtained after 15 min rest prior to exercise and at 0, 15, 30, 60 and 120 min of recovery and were analyzed for glucose, lactate, glycerol, free fatty acids, insulin, glucagons, norepinephrine (NE) and epinephrine (E). Respiratory gases were collected at 15 min intervals during exercise and at 15, 30, 45, 60, 90 and 120 min of recovery. Heart rate and mean body temperature were recorded at 10 min intervals throughout recovery. There were no differences in post-exercise oxygen consumption or heart rate while the RER suggested increased fat oxidation after exercise for the swimmers and the swimming triathletes. The mean body temperature and mean skin temperatures were significantly lower throughout 120 min of recovery for the swimmers compared to the runners. The triathletes demonstrated a similar tendency but these differences were not significant. The serum glucose levels were significantly greater (P<0.05) immediately post-exercise for the runners compared to the swimmers (6.71 +0.29 and 4.97 +0.19 mmol•1-1, respectively). Blood glucose values were also significantly greater immediately post-run for the triathletes (6.40 +0.26 and 4.87 ±0.18 mmol-l-1 for running and swimming, respectively). Blood glucose values remained elevated for runners and the running triathletes up to 30 min of recovery. Free fatty acids were similar after the run and the swim, but glycerols were increased immediately after running in the runners (P<0.05) and the triathletes (P<0.05). Differences in blood glucose levels or fat release were not explained by differences in NE, E or cortisol. The glucagon-to-insulin (G:I) ratio was significantly increased after exercise in the swimmers and the swimming triathletes. This, combined with a reduced RER after the swimming trials, suggests that the reduced glucose levels were due to reduced hepatic glycogen stores. The results of this study suggest that there were differences in substrate utilization during running and swimming exercise of the same intensity. These differences were not explained by NE, E or cortisol; however, the increased G:T ratio suggests increased carbohydrate use during exercise in the swimmers. Finally, body fat differences between runners and swimmers were not explained by differences in post-exercise energy expenditure or fat oxidation.

Although upper respiratory tract infections (URTI) are the most frequent illness among humans, insufficient evidence exists to determine if exercise training during an URTI may prolong or intensify an URTI. The purpose of this investigation was to determine the effect of exercise training on the severity and duration of URTI symptoms. Following serological screening, those subjects who were rhinovirus 16 (RV 16) antibody-free completed a graded exercise test. Thirty-four individuals (ages 18-29 years) of moderate fitness (between 32 ml/kg"1/miri 1- 60 ml/kg 1/min"1) were randomly assigned to the exercise group (EX) while 16 individuals of similar age served as a nonexercise control group (CTL). All subjects were inoculated with RV 16 on two consecutive days. EX subjects completed 40 minutes of supervised exercise at 70% of heart rate reserve within 18 hours of each inoculation and then exercised every other day for the next eight days (total of six exercise sessions). Immediately following each exercise period subjects completed a symptom checklist. EX subjects were strongly encouraged to abstain from any additional physical activity while the CTL group was encouraged to be as sedentary as possible for ten days beginning the first day of inoculation. Prior to the first inoculation and every 12 hours afterwards all subjects completed a 13 item symptom severity checklist and a physical activity log (e.g., minutes of walking, and hours of work). Used facial tissues were collected and weighed during these same reporting periods. One-way analysis of variance indicated that there! was no significant difference between groups with respect to additional physical activity. Two-way analysis of variance indicated that there were no significant differences in either the severity or duration of an URTI (symptom scores, mucous weights) between the EX and CTL groups for any given day. Further, no significant differences were observed between the pre and post exercise symptom scores for the EX group. These results suggests that moderate exercise training during a rhinovirus-caused URTI does not appear to alter the severity and duration of the illness. This was the first study to examine the influence of exercise on symptom severity and duration during an URTI. Additional studies should be performed utilizing various exercise prescriptions (e.g. intensity, frequency and duration), subject populations (e.g. younger and older), and fitness levels (e.g. sedentary, and highly fit). / School of Physical Education

Progressive myoclonus epilepsy type 1 (EPM1), also known as Unverricht-Lundborg disease, is one of the rare forms of epilepsy that shows a clear pattern of autosomal recessive inheritance. The gene defective in this disease was linked to the distal tip of chromosome 21, in band q22.3. In this study, we have collected 93 samples from 15 EPM1 families and 5 affected individuals as the basis for identifying the EPM1 gene. We have also constructed a 770 kb cosmid and bacterial artificial chromosome contig covering the candidate EPM1 region, and have isolated expressed sequences from this contig. For three of the genes that we isolated (GT335, GT334, and PWP2), we have identified and sequenced a full-length cDNA, identified the putative protein, assessed the expression pattern of the gene by Northern blot, determined the exon/intron structure of the gene, characterized basepair polymorphisms within each gene, and finally excluded each of these genes as the one defective in EPM1 patients. Using these new polymorphisms, and others that were available and that we had identified, we were able to construct detailed haplotypes on each of the affected EPM1 chromosomes, to help pinpoint, the location of the EPM1 gene, and help estimate the number of different mutations we might have in our collection. / While these studies were underway, another group identified the cystatin B (STFB) gene as that defective in EPM1. This allowed us to directly test this gene for mutations in our collection of EPM1 patients. We could identify four different mutations in the STFB gene, the most common of which consisted of a variable length insertion in the 5 ' flanking region of the gene, and which was previously undescribed. This mutation, which is found in 78% of unrelated EPM1 chromosomes we studied, showed some level of meiotic instability, and mapped to a polymorphic 12-bp GC-rich repeat. Using a combination of PCR and Southern blotting assays, we could accurately diagnose nearly 100% of all EPM1 patients. This represents a significant step forward in our ability to diagnose this disease at the molecular level, and should allow a more precise definition of the progressive myoclonus epilepsies, as a whole.

No description available.

Thesis (Ph. D.)--University of Hawaii at Manoa, 1995. / Includes bibliographical references (leaves 96-106). / Microfiche. / xxii, 106 leaves, bound 29 cm

Screening is generally considered a useful strategy in the prevention of chronic diseases. The notion is that early detection through the use of certain screening tests can facilitate effective preventive measures to be undertaken which can then lead to improved prognosis from or ultimate avoidance of serious clinical diseases. The enthusiasm for screening in the United States is high and can be seen by the size of public demand for it. Rapid technological advances and knowledge expansion in the past decade have further facilitated the introduction of new tests and screening opportunities. In the mean time, the concept of screening has undergone subtle changes. Previous emphasis on clear and demonstrable population health benefits has been slowly replaced by an emphasis on individual responsibility for the surveillance of personal health risks. Disease screening is frequently advocated as part of a health promotion programme. As a clinician who has worked in breast cancer screening and who is wary of the complexities and problems associated with disease screening, my contention is that the enthusiasm for screening may not ultimately be conducive to health and well-being. This thesis represents an effort to understand the popularity and enthusiasm for disease screening, how it has come about and, why it may not be conducive to health and well-being. The thesis begins with a description of the phenomenon to be followed by a detailed examination of the scientific principles behind disease screening. It then moves on to discover how the phenomenon might have come about by first considering the evolution of biomedicine over the centuries and then its present endeavour in the form of surveillance medicine as well as the latter�s relationship with today�s market economy. Using relevant case studies that involve, for example, cancer and prenatal genetic screening, this thesis explores different concerns relating to health and well-being, including such topics as the creation of health roles, the reconfiguration of human values and interpersonal relationships as well as medicalisation. A final chapter offers an account of health and well-being and sums up why the enthusiasm for screening may not be conducive to health and well-being. The enthusiasm for screening compels people to assume health as a moral virtue. Screening is turned into a ritual that people consume to attain salvation. Since diseases may lead to death, diseases must be avoided though screening. Yet health is not just about the absence of disease. Health and well-being can only be realised by the individual within the context of the individual�s life as a whole. The institution of biomedicine has undeniable responsibility to ensure that screening will not be used to the detriment of individuals� health and well-being. Without condemning disease screening as a potentially useful tool in the prevention of disease, this thesis advocates prudence in its utilisation. People must not be compelled to attend screening through programmes of promotion (commercially related or not). Rather, autonomous decisions must be facilitated as far as possible through the provision of clear, accurate and factual information.

Expectations from industry and the community ensure that we live in a 24-hour society. Increased demand for flexibility, capital utilisation and competition have initiated or extended shiftwork operations in many industry sectors such as mining, transport, defence, healthcare, manufacturing and security. The most common problems reported by shiftworkers are disturbed sleep and sleepiness. From a biological perspective this is not surprising because shiftworkers are often required to work at times when their mind and body are driven toward sleep. Shiftworkers may therefore be required to attempt sleep during the day; a time at which their mind and body are programmed to be awake and active. When sufficient recovery doesn't occur regularly, sleepiness and fatigue can accumulate. Traditionally, shiftwork and the work-related fatigue that it can create have been 'managed' by limiting the length of shifts and total number of hours worked per week, month or year. However, this approach fails to acknowledge key factors such as the time-of-day of work. Thus, the aim of this thesis is to provide the background, components and preliminary validations of a model to assess hours-of-work and fatigue in a scientifically appropriate manner. / thesis (PhD)--University of South Australia, 1999.