This master’s thesis explores the possibility of calculating and using the pressure andvelocity fields responsible for acoustophoresis. The goal was to use simulated data,similar to two-dimensional particle tracks from a specific microfluidic platform, toestimate the force potential and solve the partial differential equation (PDE) thatgoverns the relationship between the force potential and the acoustic fields. Lastly,the possibility of identifying the mechanical properties of unknown particles, once theacoustic field was known, was investigated. The thesis found that solving the PDE usingthe finite difference method was likely not possible and an alternative method has beensuggested. It also found that the use of particle tracks to measure the compressibilityand density of cells as a biomarker is promising, as most simulated particles wereaccurately measured. / Denna uppsats utforskar möjligheten att bestämma och använda de tryck- och hastighetsfält som är ansvariga för akustofores. Målet var att använda simulerad data, likt två-dimensionella partikelbanor för en specifik mikrofluidisk plattform, för att uppskatta kraftpotentialen och lösa den partiella differentialekvation (PDE) som styr relationen mellan kraften och de akustiska fälten. Möjligheten att bestämma mekaniska egenskaper hos okända partiklar, när det akustiska fältet är känt, utreddes också. Slutsatsen är att det inte var möjligt att lösa PDEn med hjälp av finita differensmetoden och istället föreslås en alternativ metod. Preliminära simulerade tester att karaktärisera okända partiklar gav positiva resultat, givet att de akustiska fälten är kända i två dimensioner.

In both, cell secretome analysis and bacteria evolution, controlled handling of particles with a few to sub-micrometers in size and media exchange are inevitable in order to investigate body fluid’s proteins or change the surrounding culture conditions for pivoted evolution. Typically, nanofiltration and ultra-centrifugation are employed which can lead to cell damage, need large sample volumes and have a high sample loss. Using contactless and label-free acoustic cell manipulation, disadvantages of other magnetic, dielectric or hydrodynamic methods can be avoided. Here, a novel design using acoustic forces for small particle trapping and media exchange is thoroughly numerically investigated including first- and second-order acoustic effects. The device comprises parallel aligned medium and air channels separated by a thin wall. Particle trapping occurs at this thin wall. The medium channel dimensions (height and width) and thin wall thickness are optimized with respect to trapping forces. Thinnest walls are preferable and an aspect ratio of 0.8. First preliminary experimental variation with polystyrene particles showed good agreement with the simulations. Thereby the particle trapping efficiency is evaluated under quiescent flow conditions. For particle trapping, a device with a channel height of 290μm and an aspect ratio of 0.7 is superior which supports the numerical results. Finally, medium exchange of E. coli bacteria is demonstrated with best results for a device with a channel height of 450μm and an aspect ratio of 0.8 showing that 13.4% of the initial bacteria were released after medium exchange which can be used for further processing.