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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
1

Avaliação do efeito antimicrobiano sobre Enterococcus faecalis e sua aderência promovido pelo cimento MTA com ou sem nanopartículas de prata / Evaluation of antimicrobial effect on Enterococcus faecalis and its adhesion promoted by MTA cement with or without silver nanoparticles

Cláudia Auxiliadora Pinto 27 May 2013 (has links)
Objetivo: Avaliar se a adição de nanopartículas de prata ao cimento MTA branco irá melhorar a ação antimicrobiana sobre Enterococcus faecalis e prevenir a aderência deste microrganismo ao material. Método: Teste de contato direto utilizando corpos de prova dos materiais: MTA branco (n=10) grupo B, MTA cinza (n=10) grupo C, MTA branco + NPAg em pó a 1% em peso (n=10) grupo P, MTA branco + solução de NPAg 50ppm (n=10) grupo L, que foram mantidos a 35˚C por 72 horas na suspensão de Enterococcus faecalis em SBF (0.085 UA; 660nm). Alíquotas de 100μL foram tomadas da suspensão a cada 24 horas, realizadas as diluições seriadas e semeadas em triplicata em placa de Petri contendo meio Nutriente acrescido de azul de bromotimol a 0,001%. As placas foram incubadas a 35˚C por 48 horas e então realizadas as contagens de UFC. Ao final de 72 horas, corpos de prova em resina (n=10), que haviam sido mantidos na mesma suspensão juntamente com os demais grupos, tiveram a suspensão renovada, tendo sido ajustada para 0.1 UA (660nm). Permaneceram por mais 72 horas em estufa a 35˚C e então foram submetidos ao teste de aderência. Resultados: Para a comparação intergrupos considerando a variação do número de UFC em dois períodos houve diferença significante entre o grupo P e demais grupos no intervalo (T0-24h) e no intervalo (48-72h) para o grupo C comparado ao grupo L e P (Teste Anova teste t; α=0.01). Conclusões: Os cimentos MTA cinza e branco, com ou sem nanopartículas, apresentaram ação antimicrobiana sobre Enterococcus faecalis em todos os períodos do teste de contato direto; A adição da nanopartícula de prata em pó promoveu um efeito antimicrobiano em menor tempo sobre o Enterococcus faecalis, no teste de contato direto; O MTA branco, com nanopartículas não permitiu a aderência bacteriana ao final de 72 horas em contato com a suspensão bacteriana de Enterococcus faecalis. / Aim: To evaluate if the addition of silver nanoparticles to white MTA cement will improve the antimicrobial action of Enterococcus faecalis and prevent adherence of this microorganism to the material. Method: Direct contact test using samples of materials: white MTA (n=10) B group, gray MTA (n=10) C group, white MTA + NPAG powder to 1wt% (n=10) P group, white MTA + solution NPAG of 50ppm (n=10) L group were kept at 35˚C for 72 hours in Enterococcus faecalis in SBF(0.085AU; 660nm). Aliquots of the suspension were taken every 24 hours, serial dilutions carried out in triplicate and plated on Petri dishes containing nutrient medium plus bromothymol blue 0.001%. The plates were incubated at 35˚C for 48 hours and then the CFU counts performed. At the end of 72 hours resin specimens (n=10) which had been kept at the same suspension together with the remaining groups all had renewed suspension having been adjusted to 0.1AU (660nm). Lasted for over 72 hours in an oven at 35˚C and then were tested for adhesion. Results: Comparing between those groups consider the variation of numbers of CFU in two periods there was significant differente between the P group and other groups in the interval (T0-24h) and range (48-72h) for group C compared with group L and P (Anova test - t test; α=00:01). Conclusions: The gray and white MTA, with or without nanoparticles showed antimicrobial activity on Enterococcus faecalis at all periods of direct contact test; the silver nanoparticle powder addition promoted an antimicrobial effect in less time on the Enterococcus faecalis in test direct contact; white MTA with nanoparticles prevented bacterial adherence at the end of 72 hours in contact with the bacterial suspension of Enterococcus faecalis.
2

Comparación de la resistencia compresiva del agregado Trióxido Mineral y Biodentine en perforaciones de furca de molares inferiores permanentes

Huatuco Granda, Jheymy Gerardo January 2024 (has links)
Objetivo: comparar la resistencia compresiva del agregado trióxido mineral y Biodentine TM en perforaciones de furca de molares inferiores permanentes. Materiales y métodos: se seleccionaron 30 molares inferiores humanas. Se preparó un acceso cavitario endodóntico en cada uno de los dientes para luego decoronarlos y seccionarlos eliminando las raíces, se aplicó silicona por debajo de furca y se agregó acrílico alrededor de los especímenes, empleando un molde cilíndrico. Las muestras fueron divididas aleatoriamente en 3 grupos: Grupo 1: 10 especímenes en los que se creó una perforación de furca, sellada con MTA. Grupo 2: 10 especímenes en los que se creó una perforación de furca, sellada con Biodentine TM . Grupo control negativo: 10 especímenes sin perforación. Después de 30 días, los especímenes fueron sometidos a fuerzas compresivas empleando una máquina de ensayos universales registrando la fuerza necesaria para producir la fractura. Los datos fueron procesados con las pruebas estadísticas ANOVA y Tukey, con un nivel de significancia p<0.05. Resultados: La resistencia compresiva del Biodentine (190.84 MPa) no presentó diferencias estadísticamente significativas al ser comparada con MTA (165.29 MPa) en perforaciones de furca de molares inferiores permanentes, a los 30 días de evaluación. Sin embargo, la resistencia compresiva del MTA sí presentó diferencia estadística significativa al ser comparada con el grupo control (213.46 Mpa). TM Conclusión: El Biodentine presenta valores similares al ser comparado con el grupo control y MTA. Mientras que, MTA presenta diferencias con el control, además de los valores más bajos respecto a los demás grupos. TM / Objective: to compare the compressive strength of mineral trioxide aggregate and BiodentineTM in furca perforations of permanent lower molars. Materials and methods: 30 human lower molars were selected. An endodontic cavity access was prepared in each of the teeth and then decoronated and sectioned eliminating the roots, silicone was applied under the furcation and acrylic was added around the specimens, using a cylindrical mold. The specimens were randomly divided into 3 groups: Group 1: 10 specimens in which a furcation perforation was created, sealed with MTA. Group 2: 10 specimens in which a furcation perforation was created, sealed with Biodentine . Negative control group: 10 specimens without perforation. After 30 days, the specimens were subjected to compressive forces using a universal testing machine recording the force required to produce the fracture. The data were processed with ANOVA and Tukey statistical tests, with a significance level of p<0.05. TM Results: The compressive strength of Biodentine (190.84 MPa) did not present statistically significant differences when compared to MTA (165.29 MPa) in furcation perforations of lower permanent molars at 30 days of evaluation. However, the compressive strength of MTA did show a statistically significant difference when compared to the control group (213.46 MPa). TM Conclusion: Biodentine TM presented similar values when compared with the control group and MTA. While, MTA presents differences with the control, in addition to the lower values with respect to the other groups.

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