Master of Science / Department of Agronomy / Tesfaye Tesso / Sorghum [Sorghum bicolor (L.) Moench] is listed as one of the potential feedstock sources for biofuel production. While sorghum grain can be fermented into ethanol in a similar way as maize, the greatest potential of the crop is based on its massive biomass and sugar rich juices. Thus development of the crop as alternative energy source requires improvement of these traits. The objectives of this study were (1) to determine the mode of inheritance of traits related to ethanol production and identify suitable genetic sources for use in breeding programs, and (2) to evaluate the potential of low lignin mutations for biomass feedstock production and assess biotic stress risks associated with deployment of the mutations. The study consisted of three related experiments: (i) estimating the combining ability of selected sweet and high biomass sorghum genotypes for biofuel traits and resistance to stalk lodging, (ii) determine the impact of brown mid-rib mutations on biofuel production and their reaction to infection by Macrophomina phaseolina and Fusarium thapsinum, and (iii) assess the reaction of low lignin mutants to green bug feeding. In the first experiment six sorghum genotypes of variable characteristics (PI193073, PI257602, PI185672, PI195754, SC382 and SC373) were crossed to three standard seed parent lines ATx3042, ATx623 and ATx399. The resulting hybrids and the parents were evaluated at four locations, three replications during 2009 and 2010 seasons. Data were collected on phenology, plant height, juice yield, °brix score and biomass production. In the second experiment, two brown mid-rib mutations (bmr6 and bmr12) and their normal versions were studied in four forage sorghum backgrounds (Atlas, Early Hegari, Kansas Collier and Rox Orange). The experiment was planted in four replications and at 14 d after flowering five plants in a plot were artificially infected with F. thapsinum and another five with M. phaseolina. The plants were harvested and rated for disease severity (lesion length and nodes crossed). Another five normal plants in each plot were harvested and used to determine biofuel traits (juice yield, ºbrix score and biomass). In the third experiment, a subset of entries evaluated in experiment II and three tolerant and susceptible checks were tested for greenbug feeding damage. Biotype K greenbug colony was inoculated to each genotype using double sticky foam cages. Feeding damage was assessed as percent chlorophyll loss using SPAD meter. There was significant general combining ability (GCA) effect among the male entries for juice yield, stem obrix and biomass production indicating that these traits are controlled by additive genes. Lines PI257602 and PI185672 in particular, had the highest GCA for all the traits and should serve as excellent breeding materials. There was no significant difference among the bmr mutants and between the bmr and normal genotypes for both stalk rot and greenbug damage. In conclusion, juice yield, °brix and biomass are largely controlled by additive genes and hence are amenable to genetic manipulation. The bmr mutations despite their impact on lignin content do not increase risk of attack by stalk rot pathogens and greenbugs and thus can be deployed for biofuel production without incurring losses to these factors.

Master of Science / Department of Horticulture, Forestry, and Recreation Resources / Dale J. Bremer / Jack D. Fry / Irrigation management may influence nitrate leaching under tall fescue (Festuca arundinacea) and also affect its mowing requirements. Two experiments were conducted on tall fescue growing on a Chase silt loam soil near Manhattan, Kansas. Each experiment was arranged in a split-plot design, with irrigation treatments applied to whole plots: 1) frequency-based irrigation, water was applied three times weekly to deliver a total of 19 mm water wk⁻¹ regardless of weather conditions; and 2) soil moisture sensor (SMS)--based irrigation, 34 mm of water was applied when soil dried to a predetermined threshold. In the first experiment, sub-plots consisted of unfertilized turf, and N applied as urea or polymer-coated urea at 122 and 244 kg ha⁻¹ yr⁻¹. Suction lysimeters at a 0.76 m depth were used to extract nitrate leachate bi-monthly. Turf quality was rated weekly. In the second experiment, subplots were mown at 5.1 cm or 8.9 cm, based upon the 1/3 rule, with or without monthly applications of the growth regulator trinexapac-ethyl (TE). Data were collected on total mowings and visual turf quality. Soil moisture sensor-based irrigation resulted in water savings of 32 to 70% compared to frequency-based irrigation. Leaching levels did not exceed 0.6 mg L⁻¹ and no differences in leaching were observed between irrigation treatments or among N sources. All fertilized turf had acceptable quality throughout the study. In the second experiment, irrigation strategy did not influence total number of mowings. In the first year, TE application reduced total mowings by 3 in tall fescue mowed at 5.1 cm, but only by 1.5 when mowed at 8.9 cm. In the second year, mowing at 8.9 vs. 5.1 cm or using TE vs. not resulted in a 9% reduction in total mowings each. The SMS-based irrigation saved significant amounts of water applied compared to frequency-based irrigation, while maintaining acceptable quality, but irrigation treatments did not affect nitrate leaching or mowing frequency in tall fescue on fine silt-loam soil. Nitrate leaching, regardless of amount, was well below the standards set for human health (10 mg L⁻¹). Applications of TE are more beneficial for turfgrass mowed at lower cutting heights.

Master of Science / Department of Agronomy / Nathan O. Nelson / Phosphorus (P) is an essential nutrient in crop production, but P inputs to surface waters have resulted in impairments such as eutrophication and algae blooms. Non-point sources such as agricultural fields are a main contributor of P. Kansas, being a high agricultural dependent state, has frequent fresh water body impairments. Multiple erosion and transport processes contribute to P loss. While P loss from sheet and rill erosion has been studied extensively, P loss from ephemeral gully erosion is largely unknown. The objective of this study is to understand the effects ephemeral gullies have on the transport and transformation of P. Three fields in McPherson County with well-defined ephemeral gullies were studied. Soil samples were taken in field locations that are effected by ephemeral gullies at the 0 to 2, 2 to 5, 5 to 15, and 15 to 30 cm depths. Samples were analyzed for total P, anion exchange phosphorus (AEP) (labile P), ammonium-oxalate extractable Fe, Al, and P (Fe[subscript]ox, Al[subscript]ox, P[subscript]ox), Mehlich 3 extractable Fe, Al, Ca, and P (Fe[subscript]M3, Al[subscript]M3, Ca[subscript]M3, P[subscript]M3), equilibrium phosphorus concentration at zero net sorption (EPC[subscript]0), 1:1 soil to water pH, and texture. Soil testing showed that P quantities tend to be much higher in surface soils eroded by sheet and rill erosion and lower in subsoil soil that is eroded by ephemeral gullies. The quantity of sorptive elements such as Fe and Al, were not significantly different throughout the tested area except in areas of changing soil texture. EPC[subscript]0 testing showed it was likely that P desorbs from the surface erosion of sheet and rill and is adsorbing onto the subsoil eroded from ephemeral gullies. Sediment eroded by ephemeral gullies has a P buffering capacity greater than the sediment eroded by sheet and rill, and a small quantity of ephemeral gully subsoil will have a large effect on the dissolved P concentration of runoff. Sediment, total P loss and expected dissolved P in runoff was surveyed and modeled for two of the fields. Ephemeral gullies contributed to a majority of sediment and total P loss. The addition of ephemeral gully sediment to the erosional mix of sheet and rill sediment caused the dissolved P concentration to decrease from 0.0204 to 0.0034 mg L[superscript]-1 in one field and from 0.0136 to 0.0126 mg L[superscript]-1 in another. The results of this study show that best management practices (BMPs) such as grass waterways could cause the losses of total P to decrease as much as 2 to 12 times in fields with ephemeral gullies. However, reducing ephemeral gully erosion will likely increase dissolved P concentrations up to 600% more in runoff. Therefore, BMPs need to be combined to fully control P loss from agricultural fields.

Doctor of Philosophy / Department of Agronomy / Dave Mengel / The Kansas State University Soil Testing Laboratory currently uses the Walkley-Black (WB) method for soil organic matter (SOM) estimations, the Shoemaker-Mclean-Pratt (SMP) buffer for lime recommendations, and bases the soybean phosphorus (P) critical value for P fertilizer recommendations off other crops. Hazardous waste is produced from WB and SMP creating a health hazard for workers, and substantial cost for handling and disposal. The substantial increase in land area devoted to soybean creates the need to validate currently assumed soil test P critical value and check the current P recommendations for that crop. Overarching objectives of this dissertation are to find suitable non-hazardous replacements for WB and SMP, and to find the soybean P critical value in Kansas. Three common methods used to estimate SOM are WB, dry combustion (DC), and loss on ignition (LOI). An experiment was set up using 98 Kansas soils to compare WB, scooped and weighed, LOI scooped, and DC weighed. All methods correlated well to each other with LOI to weighed WB, LOI to DC, and WB weighed to DC, having correlation coefficients of 0.97, 0.98, and 0.98, respectively. The lowest variability was observed with DC, followed by WB weighed, LOI, and then WB scooped with average standard deviations of 0.04, 0.13, 0.17, and 0.24, respectively. Two non-hazardous alternatives to the SMP buffer to determine soil lime requirement are the Sikora buffer, and the modified-Mehlich buffer. Sikora’s buffer is designed to mimic SMP. Buffer values alone or Mehlich’s equation may be used to calculate lime requirements. Thirty seven soils with a pH less than 5.8 were incubated at lime rates 0, 2240, 4480, 8960, and 17920 kg ECC ha[superscript]-1. Amount of lime required to reach pHs 6.0, 6.3, and 6.6 was calculated. Mehlich’s equation better predicted lime requirements for all target pHs and buffers than buffer pH alone. The Sikora buffer with Mehlich’s equation provided a better lime estimation than the Mehlich buffer using Mehlich’s equation. A P correlation and calibration study was conducted with soybeans at 23 sites in Eastern Kansas from 2011 to 2014. Soil Mehlich-3 P available P was compared to relative soybean yield at these sites. Soybean P critical value was found to be between 10 and 15 or 11.6 mg kg[superscript]-1 using Cate-Nelson, and linear-plateau models, respectively. A linear response to P and relative yield was observed on soils testing between 3 and 8 mg kg[superscript]-1, but not on higher testing soils.

Doctor of Philosophy / Department of Agronomy / P. V. Vara Prasad / Leguminous cover crops systems have been envisaged as a critical component of sustainable agriculture due to their potential to increase soil productivity through cycling of carbon (C) and nitrogen (N) in agricultural systems. The objectives of this study were to evaluate the performance of leguminous summer cover crops; cowpea [Vigna unguiculata (L.) Walp.], pigeon pea [Cajanus cajan (L.) Millsp], sunn hemp (Crotalaria juncea L.) and double-cropped grain crops; grain sorghum [Sorghum bicolor (L.) Moench] and soybean [Glycine max (L.) Merr.] after winter wheat (Triticum aestivum L.) and to determine the effects of these crops and varying N rates in the cropping system on nitrous oxide (N[subscript]2O) emissions, growth and yield of succeeding grain sorghum and maize (Zea mays L.) crop, soil aggregation, aggregate-associated C, and N. Field and laboratory studies were conducted for two years. The cover crops and double-cropped grain crops were planted immediately after winter wheat harvest. The cover crops were terminated at the beginning of flowering. Nitrogen fertilizer (urea 46% N) rates of 0, 45, 90, 135, and 180 kg N ha[superscript]-1 were applied to grain sorghum or maize in fallow plots. Pigeon pea and grain sorghum had more C accumulation than cowpea, sunn hemp and double-cropped soybean. Pigeon pea and cowpea had more N uptake than sunn hemp and the double-cropped grain crops. Fallow with N fertilizer application produced significantly greater N[subscript]2O emissions than all the cover crops systems. Nitrous oxide emissions were relatively similar in the various cover crop systems and fallow with 0 kg N ha[superscript]-1. Grain yield of sorghum and maize in all the cover crop and double cropped soybean systems was similar to that in the fallow with 45 kg N ha[superscript]-1. Both grain sorghum and maize in the double-cropped soybean system and fallow with 90 kg N ha[superscript]-1 or 135 kg N ha[superscript]-1 gave profitable economic net returns over the years. The double-cropped grain sorghum system increased aggregate-associated C and whole soil total C, and all the cover crop and the double-cropped soybean systems increased aggregate-associated N and soil N pools. Inclusion of leguminous cover crops without N fertilizer application reduced N[subscript]2O emissions and provided additional C accumulation and N uptake, contributing to increased grain yield of the following cereal grain crop.

Doctor of Philosophy / Department of Agronomy / Kraig L. Roozeboom / Previous research has revealed that winter wheat (Triticum aestivum L.) yields are often reduced following grain sorghum [Sorghum bicolor (L.) Moench] compared to wheat after other summer crops. The objectives of the study were to: (a) evaluate grain sorghum residue management strategies to improve the performance of a following winter wheat crop in no-till systems; (b) determine grain sorghum hybrid characteristics that facilitate planting wheat following grain sorghum, and identify winter wheat cultivars that are suitable for planting after grain sorghum; (c) evaluate effect of environment, sampling time, and grain sorghum hybrid plant pigmentation on phenolic acid concentration in sorghum residues. Experiments were conducted in environments suitable for planting winter wheat following a summer crop. Treatments for objective one were: glyphosate (pre-harvest application, post-harvest, none), residue (removed, chopped, left standing), and nitrogen (34 kg ha⁻¹ applied to residue, none). Treatments for objective two and three were grain sorghum hybrids representing three maturities (early, medium, medium-late) and two plant pigmentations (red, tan), wheat cultivars occupying significant planted acreage and having favorable performance within the region. Wheat yields increased in two environments by 217 and 630 kg ha⁻¹ when glyphosate was applied to the sorghum pre-harvest. Residue chopping or removal either had no effect or a negative effect on wheat yields compared to residue left standing. Nitrogen applied to the sorghum residue increased wheat yields in only one environment. Grain sorghum hybrid characteristics did not influence winter wheat yields in any environment, but winter wheat cultivar did influence grain yields of the winter wheat in three of the four environments. Breakdown of phenolic acids depended on environment. Results for these studies indicate that wheat yield after a grain sorghum crop can be maximized by planting a red-pigmented sorghum hybrid of an early or medium maturity, desiccating the sorghum crop with pre-harvest glyphosate if it can be applied to the sorghum roughly 45 to 50 days before a frost, and with a wheat cultivar that is well suited to no-till planting.

Master of Science / Landscape Architecture/Regional and Community Planning / Huston Gibson / We live on a planet where every three point six seconds, a life is taken from starvation or malnutrition (Millennium Development Goals, 2005). One epicenter of this tragedy is Kwara Tagi, Niger in Africa. Superficial fixes seem to leave the infrastructure of such a place in greater shambles than the original condition. The focus of this work addresses the question, “What is the potential for significantly reducing malnutrition in Kwara Tagi through sustainable intervention using Moringa trees in courtyard gardens in a manner accessible to all, without dependence on outside resources, while providing an outlet for new economic opportunity?” Rather than artificially attempting to sustain life, this work researches, articulates and suggests the potential found in a simple and strategic path of implementing Moringa trees in courtyards for the purpose of eradicating malnutrition and providing new economic opportunities. Requiring little initial funding and using resources that already exist and are not currently being utilized, this approach empowers the local people without creating any dependency on outside intervention. Furthermore, this plan would provide an economic boost to families, increase community capital, begin the reversal of desertification, work towards eradicating malnutrition, all while engaging youth in envisioning the possibilities surrounding them.

Doctor of Philosophy / Department of Agronomy / David B. Mengel / Nitrogen (N) management is one of the most recognizable components of farming both within and outside the world of agriculture. Interest over the past decade has greatly increased in improving N management systems in corn (Zea mays) and winter wheat (Triticum aestivum) to have high NUE, high yield, and be environmentally sustainable. Nine winter wheat experiments were conducted across seven locations from 2011 through 2013. The objectives of this study were to evaluate the impacts of fall-winter, Feekes 4, Feekes 7, and Feekes 9 N applications on winter wheat grain yield, grain protein, and total grain N uptake. Nitrogen treatments were applied as single or split applications in the fall-winter, and top-dressed in the spring at Feekes 4, Feekes 7, and Feekes 9 with applied N rates ranging from 0 to 134 kg ha[superscript]-1. Results indicate that Feekes 7 and 9 N applications provide more optimal combinations of grain yield, grain protein levels, and fertilizer N recovered in the grain when compared to comparable rates of N applied in the fall-winter or at Feekes 4. Winter wheat N management studies from 2006 through 2013 were utilized to develop sensor-based N recommendation algorithms for winter wheat in Kansas. Algorithm RosieKat v.2.6 was designed for multiple N application strategies and utilized N reference strips for establishing N response potential. Algorithm NRS v1.5 addressed single top-dress N applications and does not require a N reference strip. In 2013, field validations of both algorithms were conducted at eight locations across Kansas. Results show algorithm RK v2.6 consistently provided highly efficient N recommendations for improving NUE, while achieving high grain yield and grain protein. Without the use of the N reference strip, NRS v1.5 performed statistically equal to the KSU soil test N recommendation in regards to grain yield but with lower applied N rates. Six corn N fertigation experiments were conducted at KSU irrigated experiment fields from 2012 through 2014 to evaluate the previously developed KSU sensor-based N recommendation algorithm in corn N fertigation systems. Results indicate that the current KSU corn algorithm was effective at achieving high yields, but has the tendency to overestimate N requirements. To optimize sensor-based N recommendations for N fertigation systems, algorithms must be specifically designed for these systems to take advantage of their full capabilities, thus allowing implementation of high NUE N management systems.

Master of Science / Department of Agronomy / J. Anita Dille / Kraig Roozeboom / Increasing numbers of herbicide-resistant weed species require alternative methods of weed suppression to be examined. This study quantified the interaction between various cover crop or herbicide systems and horseweed [Conyza canadensis (L.)] growth. Fall cover crops of winter wheat [Triticum aestivum (L.)], winter rye [Secale cereal (L.)], barley [Hordeum vulgare (L.)] and annual ryegrass [Lolium multiflorum (L.)] were seeded in November 2012 and 2013. Spring cover crop of oat [Avena sativa (L.)] was seeded in April 2013 or rye was seeded in March 2014. All cover crops were no-till seeded into grain sorghum stubble [Sorghum bicolor (L.) Moench]. Four herbicide treatments were fall or spring applied, with and without residual. The spring non-residual treatment was also applied to plots of winter rye. Cover crop plots were split and terminated with a roller crimper or glyphosate application prior to soybean [Glycine max (L.) Merr.] planting to determine the effect of termination method on treatment performance. Soybean was planted in June 2013 and May 2014 and mechanically harvested in October of both years. Horseweed density, biomass accumulation, and soybean yield data were quantified. Horseweed height, whole plant seed production, and seed subsamples were recorded in the untreated fallow control, winter wheat, and winter rye plots in 2014. Horseweed suppression by winter rye approached 90%, levels similar to suppression by herbicide systems. In both years, herbicide plots had less than half the horseweed biomass than any of the cover crop systems. In 2013, soybean yields in herbicide plots were at least 1,500 kg ha[superscript]-1, nearly more than double yields in cover crop plots. Soybean yields in 2014 were more consistent across treatments; barley and spring rye plots achieved yields equal to or greater than 2,000 kg ha[superscript]-1. Winter rye and winter wheat reduced horseweed seed production by 60% compared to the untreated fallow control, with no effect on individual seed weight. Seed production varied across plants, with the untreated control producing the greatest number of seeds. Cover crops were successful at reducing horseweed biomass, suppressing horseweed pressure, preserving soybean biomass, and protecting soybean yields when compared to a fallow untreated control.

Doctor of Philosophy / Department of Horticulture, Forestry, and Recreation Resources / Steven Keeley / Controlling and preventing the spread of silvery-thread moss (STM, Bryum argenteum Hedw.) in putting greens is a difficult task for superintendents. Once established, a STM infestation can quickly increase through the movement of asexual propagules, such as shoot fragments, bulbils and protonema. Many practices used by superintendents are likely aiding in the dispersal and establishment of propagules. Research is needed to help superintendents effectively control this very invasive pest. The objectives of this research were to: 1) Investigate the cumulative effect of cultivation on a STM infestation in a creeping bentgrass putting green, when used with or without light, frequent topdressing and the herbicide carfentrazone; 2) Determine if STM growth is reduced by spraying ammonium sulfate (AMS) compared to urea, and understand the effect of spray volume on STM growth; 3) Evaluate the effectiveness of a range of carfentrazone rates for postemergence STM control; and 4) Determine if altering the pH of irrigation water with sulfuric- or hydrochloric acid affects the growth of STM. Generally, cultivation and carfentrazone reduced STM cover; however, the greatest reduction in STM cover was achieved when cultivation treatments were used in conjunction with carfentrazone. Topdressing did not affect STM cover. Ammonium sulfate increased STM cover and dry weight compared to urea and an untreated control. Furthermore, spray volume did not affect STM cover at any rating date. Superintendents managing STM infestations should limit or avoid use of AMS as an N source. At 28 days after treatment (DAT) the ED[subscript]90 (dose required to cause 90% gametophyte injury) was 26.8 g ai ha[superscript]-1, and at 49 DAT ED[subscript]90 was 54.3 g ai ha[superscript]-1; both of these doses are substantially lower than the label rates for long- and short-term control, respectively. As compared with label recommendations, this research suggests lower carfentrazone rates, and longer intervals, may be effective for STM control in putting greens. Irrigation pH affected STM growth, with pH’s 5 and 6 having increased growth compared to pH’s 7 and 8. It was hypothesized a lower irrigation pH enabled STM to better withstand sodium stress.