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It was suggested to me by Dr. T. Mitchell Prudden that a promising field for research existed in determining the identity of the bacterial commonly occurring in the air of New York. Very early in the investigation a practical difficulty in the way of determining species became apparent. It was found, however that the determinations of the species were not always authentic, as seen by the fact that when planted on the standard media many of them contradicted their published characters.

No description available.

Lai, Ka Ho. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2008. / Includes bibliographical references (leaves 231-241). / Abstracts in English and Chinese. / Pages / Abstract --- p.i / 摘要 --- p.iv / Acknowledgment --- p.vii / Contents --- p.viii / List of Figures --- p.xix / List of Table --- p.xxv / Abbreviations --- p.xxx / Chapter Chapter 1 --- Introduction --- p.1 / Chapter 1.1 --- Airborne fungi --- p.1 / Chapter 1.2 --- Requirement of water activity for airborne micro-organisms --- p.4 / Chapter 1.3 --- Sampling of airborne fungi --- p.5 / Chapter 1.4 --- Identification of airborne fungi --- p.10 / Chapter 1.5 --- Measurement of diversity of airborne fungi --- p.10 / Chapter 1.6 --- Airborne fungi in different environments --- p.13 / Chapter 1.7 --- Health hazards from airborne fungi and their metabolites --- p.15 / Chapter 1.7.1 --- Health hazards of airborne fungi --- p.15 / Chapter 1.7.2 --- Health hazards of mycotoxins --- p.18 / Chapter 1.7.3 --- "Mycotoxins aflatoxins, deoyxnivalenol, ochratoxins and citrinin" --- p.21 / Chapter 1.8 --- "Aim, objectives and project strategy of this study" --- p.25 / Chapter 1.8.1 --- Aim of study --- p.25 / Chapter 1.8.2 --- Objectives --- p.26 / Chapter 1.8.3 --- Strategy for the study --- p.26 / Chapter 1.9 --- Significance of the study --- p.29 / Chapter Chapter 2 --- Materials and Methods --- p.30 / Chapter 2.1 --- Sampling equipments --- p.30 / Chapter 2.1.1 --- The passive sampling methods --- p.30 / Chapter 2.1.2 --- Operation of the two passive sampling methods --- p.31 / Chapter 2.1.3 --- The active sampling methods --- p.32 / Chapter 2.1.4 --- Operation of the two active sampling methods --- p.34 / Chapter 2.2 --- Identification of airborne fungi --- p.35 / Chapter 2.2.1 --- Identification by morphological markers --- p.35 / Chapter 2.2.2 --- Molecular identification of airborne fungi --- p.36 / Chapter 2.2.2.1 --- Genomic DNA extraction from fungal biomass --- p.36 / Chapter 2.2.2.2 --- Specific polymerase chain reaction (PCR) --- p.37 / Chapter 2.2.2.3 --- Purification of PCR products --- p.38 / Chapter 2.2.2.4 --- Cycle-sequencing --- p.40 / Chapter 2.2.2.5 --- Sequencing --- p.40 / Chapter 2.2.2.6 --- Gel electrophoresis --- p.41 / Chapter 2.3 --- "Determination of viable fungal abundance, species richness, species composition and species diversity" --- p.42 / Chapter 2.3.1 --- Calculation of viable fungal abundance --- p.42 / Chapter 2.3.2 --- "Calculation of species number, relative abundance and species diversity" --- p.44 / Chapter 2.4 --- "Sampling of airborne, soilborne and dustborne fungi" --- p.45 / Chapter 2.4.1 --- Airborne fungal diversity of two storage areas sampled by four methods --- p.45 / Chapter 2.4.1.1 --- Description of the two sampling sites: mushroom house go-down in CUHK and the storage room in Tsing Yi --- p.46 / Chapter 2.4.1.2 --- Sampling conditions of the four sampling methods in the two storage rooms --- p.47 / Chapter 2.4.2 --- Airborne fungal surveys in the two construction workplaces --- p.51 / Chapter 2.4.2.1 --- Description of the construction workplaces in Tsing Yi and Castle Peak Road --- p.52 / Chapter 2.4.2.2 --- Sampling conditions for airborne fungi in the two construction workplaces --- p.58 / Chapter 2.4.2.3 --- "Determination of viable fungal abundance, species richness, species composition and species diversity in the two workplaces" --- p.59 / Chapter 2.4.3 --- Determination of the airborne fungal communities of the contractor site office with a green roof and the neighbouring government office --- p.60 / Chapter 2.4.3.1 --- "Sampling of airborne fungi from a green roof, the indoor environment of the contractor site office with a green roof and the neighboring government office" --- p.62 / Chapter 2.4.3.2 --- Sampling of soilborne fungi and determination of soil properties of the green roof --- p.62 / Chapter 2.4.3.2.1 --- Determination of microbial counts --- p.63 / Chapter 2.4.3.2.2 --- Determination of the aerial biomass of plants --- p.64 / Chapter 2.4.3.2.3 --- "Determination of soil carbon, hydrogen, nitrogen and sulfur (CHNS) contents" --- p.64 / Chapter 2.4.3.2.4 --- Analysis of soil texture --- p.65 / Chapter 2.4.3.2.5 --- Determination of total phosphorus content --- p.66 / Chapter 2.4.3.2.6 --- Determination of available nitrogen and phosphorus contents --- p.67 / Chapter 2.4.3.2.7 --- Determination of potassium content --- p.67 / Chapter 2.4.3.2.8 --- Determination of soil moisture --- p.68 / Chapter 2.4.3.2.9 --- Determination of soil pH and soil electrical conductivity --- p.68 / Chapter 2.4.4 --- Dustborne fungi and the properties of the dusts collected from the air-conditioners of the construction workplaces --- p.69 / Chapter 2.4.4.1 --- Determination of viable dustborne fungal population --- p.69 / Chapter 2.4.4.2 --- Composition analysis and toxicities of the air-conditioner dust samples --- p.70 / Chapter 2.4.4.2.1 --- "Determination of the contents of three mycotoxins by ELISA: aflatoxins, deoxynivalenol and ochratoxins" --- p.71 / Chapter 2.4.4.2.2 --- Determination of mycotoxin citrinin and volatile organic profiles by the gas chromatography-mass spectrometry (GC-MS) --- p.73 / Chapter 2.4.4.2.3 --- Determination of cytotoxicities of the dust samples --- p.75 / Chapter 2.4.4.2.3.1 --- Cell lines and culture conditions --- p.75 / Chapter 2.4.4.2.3.2 --- Growth curves of the cell lines --- p.76 / Chapter 2.4.4.2.3.3 --- Determination of the cytotoxicities of the dust extracts --- p.77 / Chapter Chapter 3 --- Result --- p.78 / Chapter 3.1 --- Airborne fungi in the workplaces --- p.78 / Chapter 3.1.1 --- Biodiversity of airborne fungi in the two storage rooms collected by four sampling methods --- p.78 / Chapter 3.1.1.1 --- Biodiversity of airborne fungi in the two storage rooms collected by the passive sampling methods --- p.78 / Chapter 3.1.1.1.1 --- Abundance of airborne fungi in the two storage rooms collected by the two passive sampling methods --- p.79 / Chapter 3.1.1.1.2 --- Species richness and composition of airborne fungi in the two storage rooms collected by the passive sampling methods --- p.81 / Chapter 3.1.1.1.3 --- Biodiversity indices of the airborne fungal communities in the two storage rooms collected by the passive sampling methods --- p.85 / Chapter 3.1.1.2 --- Biodiversity of airborne fungi in the two storage rooms collected by the active sampling methods --- p.87 / Chapter 3.1.1.2.1 --- Abundance of airborne fungi in the two storage rooms collected by the active sampling methods --- p.87 / Chapter 3.1.1.2.1.1 --- Abundances of airborne fungi in the mushroom house go-down collected by the active sampling methods --- p.88 / Chapter 3.1.1.2.1.2 --- Abundances of airborne fungi in the storage room in Tsing Yi collected by the active sampling methods --- p.94 / Chapter 3.1.1.2.2 --- Species richness in the two storage rooms collected by the active sampling methods --- p.95 / Chapter 3.1.1.2.3 --- Species compositions of airborne fungi in the two storage rooms collected by the active sampling methods --- p.98 / Chapter 3.1.1.2.4 --- Biodiversity indices of airborne fungi in the two storage rooms collected by the active sampling methods --- p.104 / Chapter 3.1.2 --- Airborne fungal surveys in the construction workplaces --- p.106 / Chapter 3.1.2.1 --- Relative humidity and temperature measured in the two construction workplaces --- p.107 / Chapter 3.1.2.2 --- Airborne fungi collected at the two workplaces --- p.108 / Chapter 3.1.2.2.1 --- Abundances of airborne fungi in the four surveys of the two construction workplaces --- p.108 / Chapter 3.1.2.2.2 --- Indoor/ outdoor (I/O) ratios of the airborne fungi in the four surveys of the two construction workplace --- p.113 / Chapter 3.1.2.2.3 --- Species richness of airborne fungi for the four surveys in the two construction workplaces --- p.114 / Chapter 3.1.2.2.4 --- Composition of the airborne fungal communities in the four surveys of the two construction workplaces --- p.116 / Chapter 3.1.2.2.5 --- Biodiversity indices of the airborne fungal communities collected in the four surveys at the two construction workplaces --- p.121 / Chapter 3.1.2.2.6 --- Properties of the identified fungi collected at the two construction workplaces --- p.124 / Chapter 3.1.3 --- The relationship between airborne fungal communities of two neighbouring offices in the Tsing Yi site --- p.125 / Chapter 3.1.3.1 --- Soilborne and airborne fungi of the green roof and their relationship with the indoor airborne fungi of the contractor site office --- p.125 / Chapter 3.1.3.2 --- The relationship between indoor airborne fungi collected from the contractor site office and the neighbouring government office --- p.127 / Chapter 3.2 --- Dusts in air-conditioner filters of the construction workplace --- p.130 / Chapter 3.2.1 --- Microscopic observation of the dust samples --- p.130 / Chapter 3.2.2 --- Viable dustborne fungi of the neighboring site offices --- p.132 / Chapter 3.2.3 --- "Mycotoxin contents, volatile organic profiles and toxicities of the dust samples" --- p.136 / Chapter 3.2.3.1 --- "Mycotoxin aflatoxins, ochratoxins and deoxynivalenol contents of the dust samples" --- p.136 / Chapter 3.2.3.2 --- Mycotoxin citrinin contents and the organic profiles of the dust samples determined by GC-MS --- p.138 / Chapter 3.2.4 --- Cytotoxicities of the dust samples --- p.156 / Chapter 3.2.4.1 --- Population growth curves of the cell lines NCI H1299 and MCF-7 --- p.156 / Chapter 3.2.4.2 --- Toxicities of the dust samples on two cell lines --- p.158 / Chapter Chapter 4 --- Discussion --- p.163 / Chapter 4.1 --- Airborne fungi in the workplace --- p.163 / Chapter 4.1.1 --- Biodiversity of airborne fungi collected in the two storage rooms --- p.163 / Chapter 4.1.1.1 --- Comparison between the airborne fungi in the two storage rooms collected by the two passive sampling methods --- p.164 / Chapter 4.1.1.1.1 --- Abundance of airborne fungi in the two storage rooms collected by the two passive sampling methods --- p.164 / Chapter 4.1.1.1.2 --- Species richness and composition of airborne fungi in the two storage rooms collected by the two passive sampling methods --- p.166 / Chapter 4.1.1.1.3 --- Species diversity of airborne fungi in the two storage rooms collected by the two passive sampling methods --- p.168 / Chapter 4.1.1.2 --- Comparison between the airborne fungi in the two storage rooms collected by the two active sampling methods --- p.170 / Chapter 4.1.1.2.1 --- Abundance of airborne fungi in the two storage rooms collected by the two active sampling methods --- p.170 / Chapter 4.1.1.2.2 --- Species richness and composition of airborne fungi in the two storage rooms collected by the two active sampling methods --- p.174 / Chapter 4.1.1.2.3 --- Species diversity of airborne fungi in the two storage rooms collected by the two active sampling methods --- p.176 / Chapter 4.1.1.3 --- Comparison between the airborne fungi in the two storage rooms collected by the passive and active sampling methods --- p.178 / Chapter 4.1.1.4 --- Conclusion on the study in the two storage rooms --- p.180 / Chapter 4.1.2 --- Airborne fungal surveys in the two construction workplaces --- p.181 / Chapter 4.1.2.1 --- Airborne fungi sampled in the two construction workplaces --- p.182 / Chapter 4.1.2.1.1 --- Relative humidity and temperature recorded in the two construction workplaces --- p.182 / Chapter 4.1.2.1.2 --- Correlation between the two environmental factors and the abundance of airborne fungi collected --- p.183 / Chapter 4.1.2.1.3 --- Airborne viable fungal sampled in the two construction workplaces --- p.184 / Chapter 4.1.2.1.3.1 --- Seasonal change of airborne fungi in the two construction workplaces --- p.184 / Chapter 4.1.2.1.3.2 --- Comparison on the viable airborne fungal abundance collected in the two construction workplaces --- p.186 / Chapter 4.1.2.1.3.3 --- Comparison on the species number and species composition collected in the two construction workplaces --- p.192 / Chapter 4.1.2.1.4 --- Comparison between indoor and outdoor airborne fungi of the two construction workplaces --- p.195 / Chapter 4.1.2.1.4.1 --- Comparison on the viable airborne fungal abundance collected in the indoor and outdoor environments of the two workplaces --- p.195 / Chapter 4.1.2.1.4.2 --- Species number and species composition collected in the indoor and outdoor environments of the two workplaces --- p.197 / Chapter 4.1.2.1.4.3 --- Species diversity collected in the indoor and outdoor environments of the two workplaces --- p.201 / Chapter 4.1.2.1.4.4 --- Properties of the identified fungi collected in the two construction workplaces --- p.202 / Chapter 4.1.2.2 --- Relationship between airborne fungal communities of two neighboring offices in the Tsing Yi site --- p.205 / Chapter 4.1.2.2.1 --- Soilborne and airborne fungi of the green roof and the airborne fungi of the contractor site office --- p.205 / Chapter 4.1.2.2.2 --- Indoor airborne fungi collected from the contractor site office and the neighbouring government site office --- p.206 / Chapter 4.2 --- Dusts from air-conditioners of the workplace --- p.207 / Chapter 4.2.1 --- Comparison of dustborne fungal abundances among different locations --- p.208 / Chapter 4.2.2 --- Comparison of dustborne fungal abundances on different accumulation times --- p.211 / Chapter 4.2.3 --- Comparison of dustborne fungal abundances on the contractor site offices and the government site office in Tsing Yi --- p.212 / Chapter 4.2.4 --- Comparison of species composition among dusts from the contractor site offices and the government site offices --- p.213 / Chapter 4.2.5 --- Mycotoxins in the dust samples --- p.214 / Chapter 4.2.5.1 --- Aflatoxin contents of the dust samples --- p.214 / Chapter 4.2.5.2 --- Deoxynivalenol contents of the dust samples --- p.215 / Chapter 4.2.5.3 --- Ochratoxin contents of the dust samples --- p.216 / Chapter 4.2.5.4 --- Mycotoxin producers in the dust samples --- p.217 / Chapter 4.2.6 --- The volatile organic profiles of the dust samples determined by GC-MS --- p.218 / Chapter 4.2.7 --- Cytotoxicities of the dust samples --- p.221 / Chapter 4.2.8 --- Conclusion on the health hazards from dusts in air-conditioners --- p.223 / Chapter 4.3 --- Sampling strategy --- p.223 / Chapter 4.4 --- Further investigation --- p.227 / Chapter Chapter 5 --- Conclusion --- p.228 / Chapter Chapter 6 --- References --- p.231 / Appendices --- p.242 / Chapter Appendix 1 --- Relative abundance of the airborne fungal species by the passive sampling method in the four surveys of the two construction workplaces --- p.242 / Chapter Appendix 2 --- Images of the most abundance fungal species --- p.246 / Chapter Appendix 3 --- Relative abundance of the airborne fungal species by the active sampling methods in the four surveys of the two construction workplaces --- p.257 / Chapter Appendix 4 --- Details of properties of fungal species identified --- p.260

Chan Pui-Ling. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2003. / Includes bibliographical references (leaves 169-182). / Text in English; abstracts in English and Chinese. / Chan Pui-Ling. / Acknowledgements --- p.i / Abstracts --- p.ii / Table of Contents --- p.v / List of Plates --- p.ix / List of Figures --- p.xii / List of Tables --- p.xiv / Abbreviations --- p.xviii / Chapter 1 --- Introduction --- p.1 / Chapter 1.1 --- Indoor Air Quality (IAQ): An overview --- p.1 / Chapter 1.1.1 --- Importance of indoor air quality --- p.2 / Chapter 1.1.2 --- Common indoor air pollutants --- p.2 / Chapter 1.1.3 --- Airborne bacteria --- p.4 / Chapter 1.1.3.1 --- Possible sources of airborne bacteria --- p.4 / Chapter 1.1.3.2 --- Health effects of the airborne bacteria --- p.5 / Chapter a. --- Sick building syndromes --- p.5 / Chapter b. --- Building-related illness --- p.7 / Chapter 1.1.4 --- Importance of studying airborne bacteria --- p.12 / Chapter 1.2 --- Situation in Hong Kong --- p.13 / Chapter 1.2.1 --- Outdoor air quality --- p.14 / Chapter 1.2.2 --- Indoor air quality --- p.14 / Chapter 1.2.2.1 --- Hong Kong studies --- p.16 / Chapter 1.2.3 --- Air quality objectives in Hong Kong --- p.18 / Chapter 1.3 --- Different sampling methods --- p.18 / Chapter 1.4 --- Identification of bacteria --- p.24 / Chapter 1.5 --- Site selection --- p.26 / Chapter 2 --- Objectives --- p.28 / Chapter 3 --- Materials and methods --- p.29 / Chapter 3.1 --- Samples collection --- p.29 / Chapter 3.1.1 --- Sampling site --- p.29 / Chapter 3.1.2 --- Complete Biosampler System --- p.29 / Chapter 3.1.3 --- Sampling preparation --- p.33 / Chapter 3.1.4 --- Sampling procedures --- p.33 / Chapter 3.2 --- Recovery of the airborne bacteria --- p.36 / Chapter 3.2.1 --- Cultural medium --- p.36 / Chapter 3.2.2 --- Recovery procedures --- p.36 / Chapter 3.2.3 --- Frozen stocks --- p.37 / Chapter 3.3 --- Indentification of bacterial strains --- p.37 / Chapter 3.3.1 --- Gram stain --- p.37 / Chapter 3.3.1.1 --- Chemical reagents --- p.37 / Chapter 3.3.1.2 --- Gram stain procedures --- p.38 / Chapter 3.3.2 --- Oxidase test --- p.38 / Chapter 3.3.2.1 --- Chemical reagents --- p.38 / Chapter 3.3.2.2 --- Oxidase test procedures --- p.41 / Chapter 3.3.3 --- Midi Sherlock® Microbial Identification System (MIDI) --- p.41 / Chapter 3.3.3.1 --- Culture medium --- p.41 / Chapter 3.3.3.2 --- Chemical reagents --- p.41 / Chapter 3.3.3.3 --- MIDI procedures --- p.41 / Chapter 3.3.4 --- Biolog MicroLogTM system (Biolog) --- p.41 / Chapter 3.3.4.1 --- Culture medium --- p.41 / Chapter 3.3.4.2 --- Chemical reagents --- p.44 / Chapter 3.3.4.3 --- Biolog procedures --- p.44 / Chapter 3.3.5 --- DuPont Qualicon RiboPrinter® Microbial Characterization System (RiboPrinter) --- p.46 / Chapter 3.3.5.1 --- Culture medium --- p.46 / Chapter 3.3.5.2 --- Chemical reagents --- p.46 / Chapter 3.3.5.3 --- RiboPrinter procedures --- p.46 / Chapter 4 --- Results --- p.50 / Chapter 4.1 --- Sample naming system --- p.50 / Chapter 4.2 --- Interpretation of results --- p.50 / Chapter 4.2.1 --- Midi Sherlock® Microbial Identification System (MIDI) --- p.51 / Chapter 4.2.2 --- Biolog MicroLog´ёØ System (Biolog) --- p.51 / Chapter 4.2.3 --- DuPont Qualicon RiboPrinter® Microbial Characterization System (RiboPrinter) --- p.52 / Chapter 4.3 --- Sample results --- p.53 / Chapter 4.3.1 --- Sample 1 (Spring) --- p.53 / Chapter 4.3.2 --- Sample 2 (Summer-holiday) --- p.62 / Chapter 4.3.3 --- Sample 3 (Summer-school time) --- p.71 / Chapter 4.3.4 --- Sample 4 (Autumn) --- p.81 / Chapter 4.3.5 --- Sample 5 (Winter) --- p.90 / Chapter 4.4 --- Bacterial profile of the student canteen --- p.100 / Chapter 4.5 --- The cell and colony morphology of the dominant bacteria --- p.100 / Chapter 4.6 --- Comparison between samples --- p.121 / Chapter 4.6.1 --- Spatial variation --- p.121 / Chapter 4.6.1.1 --- Spatial effect on bacterial abundance --- p.121 / Chapter 4.6.1.2 --- Spatial effect on species diversity --- p.121 / Chapter 4.6.2 --- Daily variation --- p.126 / Chapter 4.6.2.1 --- Daily effect on bacterial abundance --- p.126 / Chapter 4.6.2.2 --- Daily effect on species diversity --- p.126 / Chapter 4.6.3 --- Seasonal variation --- p.126 / Chapter 4.6.3.1 --- Seasonal effect on bacterial abundance --- p.126 / Chapter 4.6.3.2 --- Seasonal effect on species diversity --- p.130 / Chapter 4.7 --- Temperature effect on individual airborne bacterial population --- p.130 / Chapter 4.7.1 --- Gram positive bacteria --- p.130 / Chapter 4.7.2 --- Gram negative bacteria --- p.130 / Chapter 4.8 --- Effect of relative humidity on individual airborne bacterial population --- p.137 / Chapter 4.8.1 --- Gram positive bacteria --- p.137 / Chapter 4.8.2 --- Gram negative bacteria --- p.137 / Chapter 5 --- Discussion --- p.143 / Chapter 5.1 --- Bacterial profile --- p.143 / Chapter 5.1.1 --- Bacterial diversity --- p.143 / Chapter 5.1.2 --- Information of the identified bacteria from the student canteen --- p.144 / Chapter 5.1.3 --- Pathogenicity --- p.153 / Chapter 5.1.4 --- Summary on the bacterial profile --- p.153 / Chapter 5.2 --- Comparison between samples --- p.160 / Chapter 5.2.1 --- Spatial variation (Sampling point 1 against Sampling point 2) --- p.160 / Chapter 5.2.2 --- Daily variation (Morning against Afternoon) --- p.161 / Chapter 5.2.3 --- Seasonal variation --- p.162 / Chapter 5.2.4 --- Summer holiday against Summer school time --- p.163 / Chapter 5.2.5 --- Summary on the factors affecting the bacterial content --- p.164 / Chapter 5.3 --- Summary on indoor air quality of the student canteen in terms of bacterial level. --- p.166 / Chapter 6 --- Conclusions --- p.168 / Chapter 7 --- References --- p.169 / Appendix 1 --- p.183 / Appendix 2 --- p.187

O presente estudo tem como meta primordial investigar a temática aeromicrobiota do ambiente hospitalar, especialmente, do centro cirúrgico com vistas a enfocar contaminação biológica por conseqüência da climatização artificial, bem como fornecer informações técnicas aos profissionais afins. Assim, estabeleceu-se como objetivos específicos: quantificar e categorizar a produção do conhecimento científico nacional e internacional relacionada a aeromicrobiota do ambiente hospitalar e testar um método de avaliação microbiológica do ar do ambiente cirúrgico considerando os parâmetros biológicos da legislação nacional, e o binômio custo/benefício. As autoras evidenciaram na literatura investigada que a avaliação microbiológica do ar é expressiva nos estudos, e, quanto ao tipo de microrganismo o Aspergillus sp e o Staphylococcus sp foram os mais mencionados. Em seguida, apontam a problemática da aerocontaminação biológica e discutem as alternativas de controle da qualidade do ar. Com relação a avaliação microbiólogica do ar por meio de placas Petrifilm™ AC – Aerobic Count (3M, St Paul, MN, USA) em diferentes salas cirúrgicas ortopédicas observaram que o número de amostras positivas da entrada foi menor que na saída do ar: sala1, 0,0 na entrada e 55,6 na saída; sala 2, 40 e 83,3 e na 14 de 20,0 e 60,0%, respectivamente. Quanto ao nível de contaminação (ufc) foi expressiva apenas duas amostras da sala 2. No computo geral, os gêneros isolados foram Bacillus, Micrococcus, Actinomyces. Vale acrescentar que fungos não foram detectados. Esses resultados apontam para a eficiência do sistema de climatização. Alertam para a necessidade de uma pluralidade de ações estruturadas na multidisciplinaridade, bem como, na aplicação crítica das Resoluções e Portarias vigentes. / This study has as main goal to investigate about air microbiology of hospital environment, specially, surgical center, aiming to focus biological contamination due to artificial climate, as well as to provide technical information to professionals. So, the specific goals of this study are to quantify and classify national and international production of scientific knowledge related to air microbiology of hospital environment and test an evaluation method of air contamination, taking into account biological parameters of Brazilian legislation, and the relationship between cost and benefit. The authors have pointed out into scientific literature that air microbiological evaluation is expressive and the microorganisms Aspergillus and Staphylococcus were mentioned mostly. Next, they show the problems concerning biological contamination by air and discuss about alternative ways of controlling air quality. Regarding the microbiological air evaluation applying Petrifilm™ AC – Aerobic Count plates (3M, St Paul, MN, USA) in different orthopedic surgical rooms, the results reveal a greater number of positive samples collected at the air exit compared to those at the air entry: room 1 got 0.0 at entry and 55.6 at exit; room 2, 40 and 83.3 and room 14, 20.0 and 60.0%, respectively. The contamination level (ufc) was expressive only on two samples of room number 2. The final analysis shows that the isolated genus were Bacillus, Micrococcus and Actinomyces and fungus were not detected. These results point out the efficiency of the air conditioning system and strengthen to the need of a plurality of structured actions in the multidisciplinary field, as well as in the appliance of Brazilian legislation.

Thesis--University of Oklahoma. / Includes bibliographical references (leaves 36-37).

Thesis--University of Oklahoma. / Bibliography: leaves 36-40.

Thesis--University of Oklahoma. / Bibliography: leaves 33-36.

O presente estudo tem como meta primordial investigar a temática aeromicrobiota do ambiente hospitalar, especialmente, do centro cirúrgico com vistas a enfocar contaminação biológica por conseqüência da climatização artificial, bem como fornecer informações técnicas aos profissionais afins. Assim, estabeleceu-se como objetivos específicos: quantificar e categorizar a produção do conhecimento científico nacional e internacional relacionada a aeromicrobiota do ambiente hospitalar e testar um método de avaliação microbiológica do ar do ambiente cirúrgico considerando os parâmetros biológicos da legislação nacional, e o binômio custo/benefício. As autoras evidenciaram na literatura investigada que a avaliação microbiológica do ar é expressiva nos estudos, e, quanto ao tipo de microrganismo o Aspergillus sp e o Staphylococcus sp foram os mais mencionados. Em seguida, apontam a problemática da aerocontaminação biológica e discutem as alternativas de controle da qualidade do ar. Com relação a avaliação microbiólogica do ar por meio de placas Petrifilm™ AC – Aerobic Count (3M, St Paul, MN, USA) em diferentes salas cirúrgicas ortopédicas observaram que o número de amostras positivas da entrada foi menor que na saída do ar: sala1, 0,0 na entrada e 55,6 na saída; sala 2, 40 e 83,3 e na 14 de 20,0 e 60,0%, respectivamente. Quanto ao nível de contaminação (ufc) foi expressiva apenas duas amostras da sala 2. No computo geral, os gêneros isolados foram Bacillus, Micrococcus, Actinomyces. Vale acrescentar que fungos não foram detectados. Esses resultados apontam para a eficiência do sistema de climatização. Alertam para a necessidade de uma pluralidade de ações estruturadas na multidisciplinaridade, bem como, na aplicação crítica das Resoluções e Portarias vigentes. / This study has as main goal to investigate about air microbiology of hospital environment, specially, surgical center, aiming to focus biological contamination due to artificial climate, as well as to provide technical information to professionals. So, the specific goals of this study are to quantify and classify national and international production of scientific knowledge related to air microbiology of hospital environment and test an evaluation method of air contamination, taking into account biological parameters of Brazilian legislation, and the relationship between cost and benefit. The authors have pointed out into scientific literature that air microbiological evaluation is expressive and the microorganisms Aspergillus and Staphylococcus were mentioned mostly. Next, they show the problems concerning biological contamination by air and discuss about alternative ways of controlling air quality. Regarding the microbiological air evaluation applying Petrifilm™ AC – Aerobic Count plates (3M, St Paul, MN, USA) in different orthopedic surgical rooms, the results reveal a greater number of positive samples collected at the air exit compared to those at the air entry: room 1 got 0.0 at entry and 55.6 at exit; room 2, 40 and 83.3 and room 14, 20.0 and 60.0%, respectively. The contamination level (ufc) was expressive only on two samples of room number 2. The final analysis shows that the isolated genus were Bacillus, Micrococcus and Actinomyces and fungus were not detected. These results point out the efficiency of the air conditioning system and strengthen to the need of a plurality of structured actions in the multidisciplinary field, as well as in the appliance of Brazilian legislation.