The objective of the study is to investigate the total antioxidative capacity of plasma and the level of survival of non-activated neutrophilic leucocytes in peripheral venous blood, their oxidative function and its changes when activated by non-opsonised E.coli and S.aureus, exposed to glucose and investigate the effect of glycosylated haemoglobin (HbA1c%) of different concentrations, using the methods of luminol- and liucigenin-dependent chemiluminescence, with respect to the patients suffering from type 1 diabetes mellitus (DM) and a severe form of periodontitis, as well as those who suffer from type 1 diabetes mellitus, but have healthy periodontal tissues, and also persons without systemic diseases and inflammatory periodontal diseases. An integrated investigation of the level of oxidative capacity of neutrophilic leucocytes in peripheral venous blood, using the methods of luminol- and liucigenin-dependent chemiluminescence, of the patients who suffer from type 1 diabetes mellitus and a severe form of periodontitis as well as persons suffering from type 1 diabetes mellitus, but with no inflammatory periodontal disease, and also healthy persons, has been performed for the first time. A new presumption has been presented on the basis of the collected material, which states that the development of inflammatory diseases of periodontal tissues is determined by the decreased level of survival of bactericidal myeloperoxidase system of neutrophilic leucocytes.

Pirmą kartą atliktas asmenų grupių, turinčių skirtingą neutrofilų funkcinį aktyvumą (sergančių priedančio audinių uždegimu ir sveikų asmenų), lyginamasis periferinio kraujo neaktyvintų ir aktyvintų neutrofilų oksidacinės funkcijos tyrimas. Tyrimams buvo panaudoti labai jautrūs nuo luminolo ir lucigenino priklausomos chemiliuminescencijos metodai. Pirmą kartą įrodytas homeopatinio preparato Traumeel S antioksidacinis poveikis in vitro, reikšmingai mažinantis sergančiųjų priedančio audinių uždegimu kraujo neutrofilų generuojamo superoksido anijono kiekį. Pirmą kartą tirtas sergančių priedančio audinių uždegimu ir sveikų asmenų periferinio kraujo, plazmos ir serumo redukcinis potencialas, įvertinant homeopatinio preparato Traumeel S poveikį redukciniam potencialui in vitro. Tyrimų rezultatai papildo žinias ir apie priedančio audinių uždegimo patogenezę. / For the first time, a complex comparative study was performed on the oxidative function of non-activated and activated neutrophils of peripheral blood from subject with differing functional activity of neutrophils (i.e. patients with periodontitis and healthy subjects). The study employed two very sensitive techniques – luminol-dependent and lucigenin-dependent chemiluminescence. For the first time, the study showed that the homeopathic medication Traumeel S had antioxidant effect in vitro, i.e. it significantly reduced superoxide anion levels generated by blood neutrophils in patients with periodontitis. The results of the study also complemented knowledge about the pathogenesis of periodontal inflammation. This finding may have a significant clinical implementation.