Darbo tikslas – atlikti Baltijos šalių vietinių avių veislių: Estijos baltagalvių, Estijos juodgalvių, Estijos Ruhnu, Latvijos tamsiagalvių, Lietuvos vietinių šiurkščiavilnių, Lietuvos juodgalvių prioninio baltymo genetinės įvairovės tyrimus. Darbo uždaviniai: 1. Ištirti prioninio baltymo geno polimorfizmą 134, 157 ir 171 kodonuose, nustatyti šio geno alelius bei jų paplitimą Baltijos šalių vietinėse avių veislėse; 2. Nustatyti prioninio baltymo genotipų dažnius Baltijos šalių vietinių avių veislėse; 3. Identifikuoti prioninio baltymo genotipus pagal rizikos susirgti skrepi liga grupes, Baltijos šalių vietinių avių veislėse; 4. Sumodeliuoti atsparių skrepi ligai bandų formavimą pagal nustatytus prioninio baltymo genotipus bei jų paplitimo dažnį Baltijos šalių vietinėse avių veislėse. Pirmą kartą Baltijos šalių avių vietinėse veislėse: Estijos baltagalvių, Estijos juodgalvių, Estijos Ruhnu, Latvijos tamsiagalvių, Lietuvos vietinių šiurkščiavilnių, Lietuvos juodgalvių – buvo genotipuotas prioninio baltymo genas, identifikuoti šio geno aleliai, nustatytas jų paplitimas bei identifikuoti genotipai, pagal rizikos susirgti skrepi liga grupes. Atlikti prognoziniai skaičiavimai, siekiant suformuoti atsparių skrepi ligai avių bandas. / The aim of the work - to research prion protein gene(PrP) genetic diversity of local Baltic sheep in such breeds as Estonian whitehead, blackhead, and Ruhnu, Latvian darkhead, and Lithuanian coarsewool native, and Lithuanian blackface. Tasks of the research: 1. To investigate Prion protein gene polymorphism in 134, 157 and 171 codons, identify the gene alleles and their prevalence in the Baltic countries of the local native breeds of sheep; 2. To determine Prion protein genotype frequencies in the Baltic local native breeds of sheep; 3. To identify Prion protein genotypes according to the risk to contract scrapie disease in the native Baltic breeds of sheep; 4. To simulate scrapie resistant flocks according to the Prion protein genotypes and their prevalence rate in the native Baltic breeds of sheep. It is first time in the Baltics that the local sheep breed (Estonian whitehead, Estonian blackhead, Estonian Ruhnu, Latvian darkhead, Lithuanian coarsewool native, Lithuanian blackface) Prion protein gene was genotiped, gene alleles identified in their prevalence and genotypes classified into groups according to the risk to contract scrapie. The calculations were carried out in order to distinguish the groups of sheep resistant to scrapie.

Fojtikova 2011 INTRODUCTION: Several factors like genetic susceptibility is required for systemic rheumatic diseases development. Immunomodulatory PRL effect supports autoimmunity. AIMS: 1. To detect the immunogenetic background (alleles HLA class I, II and microsatellite polymorphism of the transmembrane part exon 5 of MIC-A gene) of SLE and PsA. 2. To detect PRL serum and synovial fluid with regard to clinical and laboratory RA activity. 3. To find the role of the functional polymorphism -1149G/T SNP PRL of extrapituitary promoter of PRL gene in SLE, RA, PsA, SSc and inflammatory myopathies development. METHODS: Genetic analyses of pateints with SLE (n=156), RA (n=173), PsA (n=100), SSc (n=75), PM (n=47) a DM (n=68) and 123 healthy individuals: PCR-SSP (HLA clase I and II), PCR-fragment analysis (MIC-A) a PCR-RFLP (-1149 G/T SNP PRL). In 29 RA a 26 OA PRL serum and synovial fluid concentrations were detected using immunoradiometric assay. RESULTS: 1. The allele HLA-DRB1*03 (pc=0.008; OR 2.5) and haplotype HLA-DRB1*03-DQB1*0201 (pc <0.001; OR 4.54) were determined as risk immunogenetic markers for SLE in Czech population. In SLE versus controls allele MIC-A5.1 was increased (pc =0.005; OR 1.88). MIC-A5.1 together with HLA-DRB1*03 increases the risk for SLE development, pc <0.000001; OR 9.71....

Fojtikova 2011 INTRODUCTION: Several factors like genetic susceptibility is required for systemic rheumatic diseases development. Immunomodulatory PRL effect supports autoimmunity. AIMS: 1. To detect the immunogenetic background (alleles HLA class I, II and microsatellite polymorphism of the transmembrane part exon 5 of MIC-A gene) of SLE and PsA. 2. To detect PRL serum and synovial fluid with regard to clinical and laboratory RA activity. 3. To find the role of the functional polymorphism -1149G/T SNP PRL of extrapituitary promoter of PRL gene in SLE, RA, PsA, SSc and inflammatory myopathies development. METHODS: Genetic analyses of pateints with SLE (n=156), RA (n=173), PsA (n=100), SSc (n=75), PM (n=47) a DM (n=68) and 123 healthy individuals: PCR-SSP (HLA clase I and II), PCR-fragment analysis (MIC-A) a PCR-RFLP (-1149 G/T SNP PRL). In 29 RA a 26 OA PRL serum and synovial fluid concentrations were detected using immunoradiometric assay. RESULTS: 1. The allele HLA-DRB1*03 (pc=0.008; OR 2.5) and haplotype HLA-DRB1*03-DQB1*0201 (pc <0.001; OR 4.54) were determined as risk immunogenetic markers for SLE in Czech population. In SLE versus controls allele MIC-A5.1 was increased (pc =0.005; OR 1.88). MIC-A5.1 together with HLA-DRB1*03 increases the risk for SLE development, pc <0.000001; OR 9.71....