Submitted by Raniere Barreto (raniere.barros@ufvjm.edu.br) on 2018-04-12T16:59:34Z No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) lara_franca_colares.pdf: 2530593 bytes, checksum: f6b37bbb6a95d44536fc10c983a32afa (MD5) / Rejected by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br), reason: Verificar: "Ara?jo e Silva" e alterar, deixando Silva. Inserir as keywords adicionado campos devidos. UFVJM n?o ? ag?ncia financiadora. on 2018-04-20T14:32:10Z (GMT) / Submitted by Raniere Barreto (raniere.barros@ufvjm.edu.br) on 2018-05-15T17:49:49Z No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) lara_franca_colares.pdf: 2530593 bytes, checksum: f6b37bbb6a95d44536fc10c983a32afa (MD5) / Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2018-05-16T11:27:14Z (GMT) No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) lara_franca_colares.pdf: 2530593 bytes, checksum: f6b37bbb6a95d44536fc10c983a32afa (MD5) / Made available in DSpace on 2018-05-16T11:27:14Z (GMT). No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) lara_franca_colares.pdf: 2530593 bytes, checksum: f6b37bbb6a95d44536fc10c983a32afa (MD5) Previous issue date: 2016 / Plantas medicinais s?o comumente usadas por comunidades tradicionais, principalmente em regi?es com menor desenvolvimento humano. Algumas esp?cies vegetais possuem entre seus componentes subst?ncias denominadas inibidores de proteases. Os inibidores de proteases se destacam na prote??o de fluidos e tecidos contra sua degrada??o por prote?lise e poss?veis falhas na degrada??o de prote?nas de meia-vida que podem interferir de forma dr?stica nas fun??es celulares. Diante do exposto, esse estudo objetivou identificar e caracterizar inibidores de proteases em cinco esp?cies vegetais nativas do Cerrado e da Mata Atl?ntica. As esp?cies de Punica granatum L. (Rom?), Plantago major L. (Tansagem), Ocimum gratissimum L. (Alfavaca), Anadenanthera colubrina Vellozo (Angico) e Stryphnodendron adstringens Mart. Coville (Barbatim?o) foram selecionados nas cidades de Pot?, Ladainha, Atal?ia, Te?filo Otoni e Ara?ua?, devido ao seu uso tradicional como anti-inflamat?rio. A sequencia gen?mica de inibidores de proteases foi pesquisada para essas esp?cies vegetais no GenBank, mas nenhuma sequencia foi descrita para as esp?cies selecionadas. As amostras provenientes dos procedimentos de extra??o foram submetidas ?s quantifica??o de prote?nas e a presen?a de inibidores de proteases foi detectada por eletroforese em gel de poliacrilamida 12% SDS-PAGE. Somente os extratos das sementes de Punica granatum e das folhas do Anadenanthera colubrina tiveram detec??o satisfat?ria de inibidores de proteases e foram submetidos ? an?lise por cromatografia l?quida de alta efici?ncia em sistema de HPLC. Este trabalho demonstra pela primeira vez a detec??o e extra??o de inibidores de proteases em folhas de Anadenanthera colubrina e sementes de Punica granatum. / Disserta??o (Mestrado Profissional) ? Programa de P?s-Gradua??o em Tecnologia, Sa?de e Sociedade, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2016. / Traditional communities, especially in regions with lower human development, commonly use medicinal plants. Some plant species have among their components substances called protease inhibitors. Protease inhibitors act protecting fluids and tissues from degradation by proteolysis and possible failures in the degradation of half-life proteins that can drastically interfere with cellular functions. This study aimed to identify and characterize protease inhibitors in five native plant species of Cerrado and Atlantic Forest. The species of Punica granatum L. (Rom?), Plantago major L. (Tansagem), Ocimum gratissimum L. (Alfavaca), Anadenanthera colubrina Vellozo (Angico) and Stryphnodendron adstringens Mart. Coville (Barbatim?o) were selected in the cities of Pot?, Ladainha, Atal?ia, Te?filo Otoni and Ara?ua? due to their traditional use. The genomic sequence of protease inhibitors was screened for these plant species in GenBank, but no sequence was described for the selected species. Samples from the extraction procedures were subjected to protein quantification and the presence of protease inhibitors was detected by 12% SDS-PAGE polyacrylamide gel electrophoresis. Only the extracts of the seeds of Punica granatum and of the leaves of Anadenanthera colubrina had satisfactory detection of proteases inhibitors and were submitted to the analysis by high performance liquid chromatography system. This work demonstrates for the first time the detection and extraction of protease inhibitors in leaves of Anadenanthera colubrina and seeds of Punica granatum.