The aim of master´s thesis is to assess the financial situation of company ČEZ. In the first part, there are prepared a theoretical basis of elementary and statistical methods of financial analysis, which is followed by the introduction of the company. In the second part, there is with using methods of financial analysis performed evaluation of financial situation of the company and the results are compared with competition.

This thesis analyses the financial situation of the company Královopolská RIA, a.s. in the years 2002 to 2006 at the basis of sesected methods of the financial analysis. It comprieses proposals of possible solutions of indentified problems which should result in the improvement of financial situation of the company in future years.

<p>Predviđena istraživanja u ovoj doktorskoj disertaciji su usmerena u pravcu&nbsp;<br />modifikacije klasičnih metoda i postupaka za transformaciju karboksilne grupe&nbsp;<br />prirodnih i sintetičkih karboksilnih kiselina. Modifikacije su rađene novim,&nbsp;<br />savremenim, ekonomski i ekolo&scaron;ki opravdanim metodama rada u&nbsp;<br />mikrotalasnom reaktoru. Modifikovanim sintetskim postupcima u&nbsp;<br />mikrotalasnom reaktoru urađena je sinteza amida, hidroksamskih&nbsp; derivata,&nbsp;<br />kao i redukcija individualnih naftnih kiselina i&nbsp; sme&scaron;e&nbsp; prirodnih naftnih kiselina&nbsp;<br />&bdquo;Velebit&ldquo; do alkohola. Prirodne naftne kiseline kori&scaron;ćene u ovom radu su prvo&nbsp;<br />izolovane iz gasne frakcije (interval destilacije 168-290 &deg;C) vojvođanske nafte&nbsp;<br />&bdquo;Velebit&ldquo; a potom preči&scaron;ćene i razdvojene na uže frakcije na osnovu različite&nbsp;<br />kiselosti. Nakon toga, izvr&scaron;ena je njihova karakterizacija GC-MS-EI analizom&nbsp;<br />(čime je potvrđeno da dolazi do strukturne diferencijacije kiselina). U radu je&nbsp;<br />takođe ispitana biolo&scaron;ka aktivnost sintetizovanih derivata. Proučavan je uticaj&nbsp;<br />prirodnih naftnih kiselina &bdquo;Velebit&ldquo; i njenih derivata na rast&nbsp; pet sojeva&nbsp;<br />Pseudomonas sp., kao i uticaj odabranih sintetisanih jedinjenja na proliferaciju&nbsp;<br />četiri ćelijske linije humanih tumora pri čemu je kao kontrola služila jedna&nbsp;<br />zdrava humana ćelijska linija.</p> / <p>The investigation of this doctoral dissertation is directed toward the modification of the &nbsp;transformation of the carboxylic group of natural and synthetic carboxylic acids. The dissertation takes into consideration the classical methods and procedures of the reaction and modifies them using microwave reactor. The synthesis of amides, hydroxamic derivatives, as well as the reduction&nbsp; of individual petroleum acids and acid mixtures of natural oil &quot;Velebit&quot; to alcohol were achieved&nbsp; by the modifications made to the synthetic methods in the microwave reactor. The natural oil acids used within this study were first isolated from the gas fraction (distillation interval 168-290 &deg;C) of the Vojvodina&#39;s crude oil &quot;Velebit&quot;, and then purified and separated by the narrow fractions under the different acidity. After that, their characterisation was made by the GC-MS-EI analysis which confirmed that the structural differentiation of&nbsp;&nbsp; acids had been achieved. Also, the biological activity of the synthesized derivatives are analysed. The impact of natural petroleum acids &quot;Velebit&quot; and its derivatives on the growth of five strains of&nbsp; Pseudomonas&nbsp; sp. was studied, as well as the impact of selected synthesized compounds on the proliferation of four human tumor cell lines wherein one healthy human cell lines used as the control.</p>

<p>Steroidna jedinjenja spadaju u grupu supstanci sa &scaron;irokim spektrom biolo&scaron;kog delovanja i predstavljaju dobru polaznu osnovu za sintezu mnogih derivata sa željenim biolo&scaron;kim potencijalom. Organskim sintezama se do&scaron;lo do velikog broja steroidnih derivata, od kojih su neki pokazali značajnu biolo&scaron;ku aktivnost, kao &scaron;to je citotoksičnost prema različitim ćelijskim linijama kancera. Karakterizacija novosintetisanih jedinjenja može se izvesti eksperimentalnim i računarskim (in silico) metodama. U ovoj doktorskoj disertaciji predstavljeno je eksperimentalno određivanje lipofilnosti 17&alpha;-pikolil i 17(E)-pikoliniliden androstanskih derivata primenom visokopritisne tečne hromatografije na obrnutim fazama, a potom hemometrijska analiza hromatografskog pona&scaron;anja (hromatografske lipofilnosti) QSRR pristupom. Hemijska struktura analiziranih derivata opisana je numerički, pomoću izračunatih molekulskih deskriptora. U drugom delu doktorske disertacija predstavljena je QSAR analiza citotoksične aktivnosti 17&alpha;-pikolil i 17(E)-pikoliniliden androstanskih derivata prema ćelijama androgen-receptor negativnog kancera prostate (AR-neg. PC-3). Odabir najkvalitetnijih QSRR i QSAR modela obavljen je na osnovu izračunatih statističkih parametara, a njihovo rangiranje izvedeno je primenom metode sume razlika rangova (SRD). Pored regresionih QSRR i QSAR hemometrijskih metoda, primenjene su i klaster analiza i analiza glavnih komponenata sa ciljem utvrđivanja sličnosti ili razlika između analiziranih derivata na<br />osnovu izračunatih molekulskih deskriptora.</p> / <p>Steroidal compounds belong to the group of substances with wide spectrum of biological activity and represent the basic material for synthesis of many derivatives<br />with preferred biological potential. A grate number of steroidal derivatives have been<br />obtained through organic syntheses, many of which have demonstrated significant<br />biological activity, such as cytotoxicity toward various cancer cell lines. Characterization of newly synthesized compounds can be achieved experimentally<br />and by computational approach (in silico). This doctoral dissertation describes<br />experimental determination of lipophilicity of 17&alpha;-picolyl and 17(E)-picolinylidene<br />androstane derivatives applying reversed-phase high pressure liquid chromatography followed by quantitative structure-retention relationship (QSRR)<br />chemometric analysis of chromatographic behaviour (chromatographic lipophilicity).<br />Chemical structure of the analyzed derivatives was described numerically by in silico<br />molecular descriptors. The second part of this dissertation describes quantitative<br />structure-activity relationship (QSAR) analysis of cytotoxic activity of 17&alpha;-picolyl and<br />17(E)-picolinylidene androstane derivatives toward androgen-receptor negative<br />prostate cancer cell line (AR-neg. PC-3). Selection of the best QSRR and QSAR<br />models was carried out based on their statistical parameters, and their ranking was<br />done by sum of ranking differences (SRD) method. Besides the regression QSRR and QSAR chemometric methods, cluster analysis and principal components analysis were conducted in order to reveal possible similarities and dissimilarities among the studied derivatives on the basis of calculated molecular descriptors.</p>

<p>Opisane&nbsp; su&nbsp; sinteze&nbsp; novih&nbsp; liganada&nbsp; bis(ftalazin-1hidrazon)-2,6-diacetilpiridna&nbsp; (Hz₂DAP&middot;2HCl),&nbsp; bis(3-hlorpiridazin-6-hidrazon)-2,6-diacetilpiridina (Hp₂DAP),&nbsp; 3-hlorpiridazin-6-hidrazon&nbsp; di(2-piridil) ketona&nbsp; (HpDPK),&nbsp; ftalazin-1-hidrazon&nbsp; di(2-piridil)<br />ketona&nbsp; (HzDPK)&nbsp; i&nbsp; ftalazin-1-hidrazon&nbsp; piridin-2-karbaldehida&nbsp; (HzPY).&nbsp; Zajedničko&nbsp; svojstvo&nbsp; dobijenih liganada&nbsp; je&nbsp; &scaron;to&nbsp; sadrže&nbsp; piridinski&nbsp; i&nbsp; diazinski&nbsp; prsten&nbsp; i sadrže&nbsp; samo&nbsp; donorne&nbsp; atome&nbsp; azota.&nbsp; Tokom&nbsp; nastajanja kompleksa dolazi do deprotonacije liganada. Svi ligandi su&nbsp; okarakterisani&nbsp; elementalnom&nbsp; analizom, termoanalitičkim&nbsp; metodama&nbsp; i&nbsp; metodom&nbsp; IR spektroskopije,&nbsp; dok&nbsp; neki&nbsp; i&nbsp; metodom&nbsp; NMR spektroskopije&nbsp; kao&nbsp; i&nbsp; rendgenskom&nbsp; strukturnom analizom.Za&nbsp; sintezu&nbsp; koordinacionih&nbsp; jedinjenja&nbsp; primenjeni&nbsp; soli Co(II),&nbsp; Ni(II),&nbsp; Cu(II)&nbsp; i&nbsp; Zn(II).&nbsp; Dobijeni&nbsp; kompleksi su okarakterisani&nbsp; elementalnom&nbsp; analizom, konduktomerijskim&nbsp; i&nbsp; magnetnim&nbsp; merenjima,&nbsp; IR spektroskopijom i termoanalitičkim metodama. Barem jedan&nbsp; kompleks&nbsp; iz&nbsp; svake&nbsp; serije&nbsp; je&nbsp; okarakterisan&nbsp; i rendgenskom strukturnom analizom. Urađena&nbsp; su&nbsp; i&nbsp; ispitivanja&nbsp; antimikrobne&nbsp; aktivnosti odabranih&nbsp; jedinjenja&nbsp; prema&nbsp; predstavnicima&nbsp; grampozitivnih i gram-negativnih bakterija i kulturu kvasca. Pored&nbsp; toga,&nbsp; urađena&nbsp; su&nbsp; i&nbsp; ispitivanja&nbsp; citotoksične,antiproliferativne&nbsp; i&nbsp; inhibitorne&nbsp; aktivnosti&nbsp; jedinjenja prema&nbsp; roditeljskim&nbsp; i&nbsp; multirezistentnim&nbsp; T-limfomnim ćelijama&nbsp; kancera.&nbsp; Utvrđeno&nbsp; je&nbsp; da&nbsp; neka&nbsp; jedinjenja pokazuju&nbsp; izrazito&nbsp; mikrobicidno,&nbsp; citotoksično, antiproliferativno i inhibitorno dejstvo.</p> / <p>The synthesis of new ligands dihydrochloride salt of 2,6-diacetylpyridne&nbsp;&nbsp; bis(phthalazine-1hydrazone) (Hz₂DAP&bull;2HCl),&nbsp; 2,6-diacetylpiridine&nbsp; bis(3- chloropyridazine-6-hydrazone)&nbsp; (Hp₂DAP),&nbsp; di(2-pyridyl)ketone&nbsp; 3-chloropyridazine- 6-hydrazone (HpDPK),&nbsp; di(2-pyridyl)ketone&nbsp; phthalazine-1-hydrazone&nbsp; (HzDPK)&nbsp; and&nbsp; &nbsp; pyridine-2-carbaldehide phthalazine-1-hydrazone&nbsp; (HzPY)&nbsp; have&nbsp; been described. All the ligands contain pyridine and diazine core and all of them have only nitrogen donor atoms. During the complex formation the deprotonation of ligands takes&nbsp; places.&nbsp; All&nbsp; of&nbsp; the&nbsp; ligands&nbsp; have&nbsp; been characterized&nbsp; by&nbsp; elemental&nbsp; analysis,thermoanalytical&nbsp; methods&nbsp; and&nbsp; IR&nbsp; spectroscopy.&nbsp; In some&nbsp; cases&nbsp; also&nbsp; by&nbsp; NMR&nbsp; spectroscopy&nbsp; and&nbsp; X-ray structural analysis.Co(II), Ni(II), Cu(II) and Zn(II) salts were&nbsp; used for the synthesis&nbsp; of&nbsp; the&nbsp; coordinational&nbsp; compounds.&nbsp; The obtained complexes were characterized by elemental analysis,&nbsp; molar&nbsp; conductivity&nbsp; and&nbsp; magnetic measurements,&nbsp; IR&nbsp; spectroscopy&nbsp; and thermoanalytical&nbsp; methods.&nbsp; At&nbsp; least&nbsp; one complex&nbsp; of each&nbsp; series&nbsp; were&nbsp; characterized&nbsp; by&nbsp; X-ray&nbsp; structural analysis.The antimicrobial activity of some of the compounds toward&nbsp; Gram-positive/Gram- negative&nbsp; bacteria furthermore,&nbsp; the&nbsp; cytotoxic,&nbsp; antiproliferative&nbsp; and inhibitory&nbsp; activity&nbsp; toward&nbsp; sensitive&nbsp; parental&nbsp; andmultiresistant&nbsp; T-lymphoma&nbsp; cancer&nbsp; cells&nbsp; have&nbsp; also been carried out. It&nbsp; can&nbsp; be&nbsp; concluded&nbsp; that&nbsp; some&nbsp; of&nbsp; the&nbsp; compounds exhibit&nbsp; outstanding&nbsp; antimicrobial,&nbsp; cytotoxic, antiproliferative, and inhibitory activity.</p>

<p>&nbsp;&nbsp; U ovoj doktorskoj disertaciji opisane su sinteze novih kompleksa prelaznih metala sa piridoksiliden-&nbsp; (PLAG), odnosno saliciliden-aminogvanidinom (SALAG). Dobijeni<br />kompleksi su okarakterisani elementalnom analizom, IR spektrima, konduktometrijskim i magnetnim merenjima, a većina i rendgenskom<br />strukturnom analizom. Osim toga, dobijene su i nove forme ovih &Scaron;ifovih baza, i to u vidu monokristala, čime su omogućena ispitivanja njihovih&nbsp; molekulskih i kristalnih struktura, kao i uporedna analiza sa koordinovanim ligandima.<br />&nbsp; &nbsp; Sa PLAG je sintetisano 7 novi&nbsp; kompleksa Cu(II), a pored toga po prvi put su<br />izolovani mono i bis(ligand) kompleksi Fe(III) i Co(III), mono(ligand) kompleksi&nbsp; V(V), kao&nbsp; i jedan kompleks Zn(II) u kojem ovaj potencijalno tridentatni&nbsp;<em> ONN&nbsp;</em> ligand,&nbsp; u<br />monoprotonovanoj formi,&nbsp; ima ulogu kontra-jona. Sa stanovi&scaron;ta geometrije zajedničko za izolovane komplekse Cu(II) i V(V) je da imaju kvadratno-piramidalnu strukturu, sa izuzetkom jednog kvadratno-planarnog kompleksa Cu(II), dok je u kompleksima Fe(III) i Co(III) nađeno očekivano oktaedarsko okruženje centralnog<br />jona. Pored ovih, sintetisano je i pet novih kompleksa sa SALAG, od kojih su dva<br />kompleksa Cu(II) i kompleks V(V) okarakterisani rendgenskom strukturnom<br />analizom, dok je mikrokristalnim bis(ligand) kompleksima Co(III) i Ni(III) na osnovu<br />fizičko-hemijskih karakteristika predložena odgovarajuća struktura.<br />&nbsp;&nbsp; Zajedničko za obe opisane &Scaron;ifove baze je da se koordinuju na&nbsp;<em> ONN&nbsp; </em>tridentatni način, i to preko atoma kiseonika deprotonovane fenolne grupe i atoma azota azometinske i imino grupe AG fragmeta. Posebno je nagla&scaron;eno da su&nbsp; sa<br />PLAG izolovana dva dimerna kompleksa Cu(II) u kojima je po prvi put nađena tetradentatna koordinacija ovog&nbsp; liganda, u koju je dodatno uključen atom kiseonika hidroksimetil-grupe PL-ostatka. Za razliku od SALAG, koji je u izolovanim kompleksima koordinovan isključivo kao monoanjon, nastao deprotonacijom fenolne<br />OH-grupe, za PLAG je osim ove, potvđena koordinacija u neutralnoj, zwitter-jonskoj, ali i dvostruko deprotonovanoj formi. Zwitter-jonska forma liganda nastaje migracijom atoma vodonika sa fenolnog hidroksila na piridinski atom azota PL-ostatka, dok deprotonacijom piridinskog ili hidrazinskog atoma azota, odnosno oba pomenuta atoma nastaju monoanjon i dianjon helatnog liganda, respektivno.<br />&nbsp;&nbsp; Na kraju, urađena su&nbsp; i ispitivanja antimikrobne aktivnosti odabranih jedinjenja<br />prema predstavnicima grampozitivnih i gramnegativnih bakterija, kao i dve kulture<br />kvasca. Tom prilikom nije utvrđena nikakva inhibitorna aktivnost&nbsp;&nbsp; prema primenjenim<br />bakterijskim sojevima, dok su u slučaju kvasaca izvesno mikrobicidno dejstvo pokazali samo kompleksi Cu(II).</p> / <p>This PhD thesis describes the syntheses of some new transition metal complexes with&nbsp; pyridoxilidene-&nbsp; (PLAG) and salicylideneaminoguanidine (SALAG). Obtained&nbsp; complexes are characterized by&nbsp;&nbsp; elemental analysis, IR&nbsp; spectroscopy,&nbsp; conductometric and magnetic measurements. Besides, the structural analysis of majority of the obtained complexes was performed. Some new forms of these Schiff&nbsp; bases are synthesized in form of single crystals, which made their X-ray analysis as well as comparison with coordinated forms possible.</p><p>With PLAG, 7 new Cu(II) complexes were obtained and for the first time mono and bis(ligand) complexes of Fe(III) and Co(III) as well&nbsp; mono(ligand)&nbsp; complexes&nbsp; of V(V) were isolated. Furthermore,&nbsp; the structure of Zn(II) complex in which PLAG in its monocationic form has a role of counter ion is presented. With the exception of one Cu(II) complex, all reported Cu(II) and V(V) complexes have a square-pyramidal geometry, whilst&nbsp; Fe(III)&nbsp; and Co(III)&nbsp; are situated in octahedral surroundings. Also, five new complexes of Cu(II), Co(III), Ni(II) and V(V) with SALAG were synthesized. In both&nbsp; Cu(II) complexes and V(V) complex the expected coordination mode and geometry were confirmed by X-ray analysis, while octahedral structure of bis(ligand) complexes with Co(III) and Ni(II) was proposed based on results of physico-chemical&nbsp; characterization.</p><p>Both PLAG and SALAG coordinate the metal ion in tridentate&nbsp; ONN&nbsp; manner, through the oxygen atom of deprotonated phenolic group and nitrogen atoms&nbsp; of azomethine and imino groups of AG moiety. It is also emphasized that in two dimeric Cu(II) complexes with PLAG tetradentate coordination mode was found, in which the oxygen atom of hydroxymethyl group of PL residue was additionally involved. Unlike SALAG, which is coordinated as monoanion in all of the examined complexes, PLAG can have one of three degrees of deprotonation. Zwitter-ion of PLAG is formed by migration of H-atom from phenolic oxygen to pyridine nitrogen, while the deprotonation of pyridine or/and hydrazine nitrogen, makes it mono-and dianion, respectively.</p><p>Also, microbiological tests on the selected compounds were preformed. Namely, antimicrobial activity of these compounds against some gram-positive and gram-negative bacteria, as well as some yeast cultures was examined and none of the samples showed antimicrobial activity against bacteria, whilst only Cu(II) complexes showed certain inhibitory effect against yeasts.</p>

<p>U okviru ove disertacije ispitan je hemijski, mineralni i<br />polifenolni sastav, kao i biolo&scaron;ki potencijal četiri sorte<br />kupina sa dva različita lokaliteta sjeverozapadnog dijela<br />Bosne i Hercegovine, sa lokaliteta Verići (divlja i<br />kultivisana sorta Čačanska bestrna) i sa lokaliteta<br />Javorani (divlja sorta i kultivisana sorta Chester<br />Thornles). Određen je sadržaj suve materije, pepela,<br />sirove celuloze, ukupnih &scaron;ećera, ukupna kiselost i sadržaj<br />askorbinske kiseline. Sadržaj mineralnih materija<br />detektovan je metodom optičke emisione spektrometrije<br />(ICP-OES). Za izdvajanje ekstrakata je kori&scaron;tena klasična<br />ekstrakciona tehnika prema Soxhlet-u uz upotrebu 80 %<br />etanola (v/v). Spektrofotometrijskim metodama određen<br />je sadržaj ukupnih polifenola, flavonida, flavonola,<br />ukupnih i monomernih antocijana. Kvalitativnom i<br />kvantitativnom HPLC metodom je utvrđen sadržaj<br />pojedinačnih polifenolih jedinjenja. Biolo&scaron;ki potencijal<br />uzoraka je utvrđen u sistemima in vitro, gdje je određena:<br />antioksidativna aktivnost, antihiperglikemijska aktivnost,<br />antiproliferativni efekat i antimikrobna aktivnost.<br />Antioksidativna aktivnost uzoraka je ispitana sa četiri<br />metode: DPPH test, ABTS test, sposobnost neutralizacije<br />hidroksil radikala i metodom inhibicije Briggs Rauscherovih oscilatornih reakcija. Antihiperglikemijska aktivnost<br />ispitivanih uzoraka kupine je dokazan na osnovu<br />sposobnosti inhibicije enzima &alpha;-glukozidaze.<br />Antiproliferativni efekat ispitivanih uzoraka je određen<br />prema inhibiciji rasta četiri humane ćelijske linije: HeLa<br />(epitelnog karcinoma cerviksa), HT-29 (adenokarcinoma<br />debelog crijeva), MRC-5 (zdravim ćelijama fibroblasti<br />pluća) i prema ćelijskoj liniji MCF7 (adenokarcinoma<br />dojke). Antimikrobna aktivnost je utvrđena prema gram<br />pozitivnom soju (G⁺) bakterija S. Aureus i gram<br />negativnom (G^-) soju E. coli, rastu micelija Aspergillusa<br />niger i Candida albicans. Regresionom analizom prema<br />Pirson-u su određeni odnosi između sadržaja polifenolnih<br />jedinjenja i biolo&scaron;kog potencijala, sa statističkim<br />značajem (p &le; 0,01).</p> / <p>The chemical, mineral, polyphenolics composition and<br />biological potentials of four blackberries varieties from two<br />different locations in the northwestern part of Bosnia and<br />Herzegovina (Verići - wild and cultivated variety Čačanska<br />bestrna and Javorani - wild and cultivated variety Chester<br />Thornless) were determined. The contents of dry matter, ash,<br />crude cellulose, total sugars, total acidity and ascorbic acid were<br />obtained. The contents of mineral matter were detected by<br />optical emission spectrometry (ICP-OES) method. A classic<br />Soxhlet extraction technique with 80% ethanol (v/v) was<br />applied to obtain extracts. The spectrophotometric methods<br />were used to determine the content of total polyphenolics,<br />flavonoids, flavonols, and total and monomer anthocyanins. The<br />content of individual polyphenolic compounds was determined<br />by HPLC method. The biological potentials (antioxidant<br />activity, antihyperglycemic activity, antiproliferative effect and<br />antimicrobial activity) of the samples were determined in vitro<br />systems. The antioxidant activity was tested with four methods:<br />DPPH test, ABTS test, ability to neutralize OH radicals and the<br />method of inhibiting Briggs Rauscher oscillatory reactions. The<br />antihyperglycaemic activity of the tested blackberry samples<br />was based on the ability to inhibit &alpha;-glucosidase enzyme. The<br />antiproliferative effect of the tested samples was determined by<br />inhibiting the growth of four human cell lines: epithelial<br />carcinoma of the cervix (HeLa), colon adenocarcinoma (HT-<br />29), healthy lung fibroblast cells (MRC-5) and the cell line of<br />breast adenocarcinoma (MCF7). The extracts showed the<br />highest inhibitory effect on the cell line of breast<br />adenocarcinoma (MCF-7). The antimicrobial activity was<br />determined according to gram-positive bacteria (G ⁺) of<br />Staphylococcus aureus, Gram-negative bacteria (G ^-)<br />Escherichia coli, growth of mycelium Aspergillus niger and<br />fungi Candida albicans. The Pearson correlations were<br />statistically determined the relationship between the content of<br />polyphenolic compounds and biological potential, with<br />statistical significance (p &le; 0.01).</p>

he aim of this thesis is to carry out an appraisal of a financial condition of a company. In the first part, theoretical start-points of Financial Analysis are scrutinised, with an introduction of the company followed. By taking the advantage of the Financial Analysis method, the financial condition of the company is appraised and the outcomes are compared with competition. In the second part, proceedings for risk elimination in the company management are suggested.