Part of Thesis (Ph. D.)--University of Michigan, 1941. / Cover title. "By F.F. Blicke and Chieko Otsuki." "Contribution from the College of pharmacy, University of Michigan." "Reprinted from the Journal of American chemical society, 63 ... (1941)."

Part of Thesis (Ph. D.)--University of Michigan, 1941. / Cover title. "By F.F. Blicke and Esperanza R. Castro." "Contribution from the College of Pharmacy, University of Michigan." "Reprinted from the Journal of the American Chemical Society, 63 ... (1941)."

Part of Thesis (Ph. D.)--University of Michigan, 1941. / Cover title. "By F.F. Blicke and Chieko Otsuki." "Contribution from the College of pharmacy, University of Michigan." "Reprinted from the Journal of American chemical society, 63 ... (1941)."

Thesis (Ph. D.)--University of Massachusetts Amherst, 2009. / Open access. Includes bibliographical references (p. 85-104). Print copy also available.

Thèse--Faculté de medecine de Paris. / "Index bibliographique": p. [45]-47.

Thesis (PH. D)--University of Chicago, 1921. / "Author's abstract of this paper issued by the [Wistar institute] Bibliographic service, May 1." "Private edition, distributed by the University of Chicago libraries, Chicago, Illinois." "Reprinted from the Journal of experimental zoology, vol. 36, no. 1, July, 1922." Bibliography: p. 46-47. Also available on the Internet.

Orientadores: Jose Ranali, Eneida de Paula / Tese (doutorado) - Universidade Estadual de Campinas, Faculdade de Odontologia de Piracicaba / Made available in DSpace on 2018-08-09T14:14:31Z (GMT). No. of bitstreams: 1 Tofoli_GiovanaRadomille_D.pdf: 1959024 bytes, checksum: 41e36ed1b20143ae986df234590b8fac (MD5) Previous issue date: 2007 / Resumo: Este trabalho comparou a reação tecidual provocada por soluções comerciais de mepivacaína 2% com epinefrina 1:100.000 (MVC 2% EPI), mepivacaína 3% sem vasoconstritor (MVC 3%), e preparação lipossomal de mepivacaína 2% (MVC LUV 2%), através do teste de edema de pata, em ratos, avaliado 60, 120 e 180 minutos após a injeção destas formulações. Além disso, a reação tecidual na mucosa oral de ratos provocada pela injeção destas formulações, após 6 horas, 24 horas e 4 dias foi analisada por técnicas histológicas. Os níveis plasmáticos de mepivacaína obtidos após a injeção intra-oral destas formulações em ratos foram determinados com espectrometria de massas (CLAE-EM-EM), após 0, 15, 30, 45, 60, 120, 180, 240, 300, 360 e 420 minutos da injeção. O teste de edema de pata não mostrou diferenças estatisticamente significantes entre as formulações testadas em nenhum dos tempos analisados (p>0,05). De forma geral, considerando cada um dos tempos em estudo, a avaliação histológica mostrou que a MVC LUV 2% promoveu reação inflamatória de menor intensidade quando comparada com a MVC 2% EPI (p<0,05), mas não mostrou diferenças com relação à MVC 3%. Os níveis plasmáticos obtidos após a injeção de MVC LUV não apresentaram diferença estatisticamente significativa daqueles obtidos após a injeção de MVC 2% EPI (p>0,05), porém a injeção de MVC LUV promoveu concentrações menores que aquelas obtidas após a injeção de MVC 3% até os 240 minutos (p<0,05). Diante dos resultados obtidos, podemos concluir que a formulação lipossomal de mepivacaína promoveu reação inflamatória menor que a promovida pela formulação com epinefrina na mucosa oral. Além disso, a encapsulação do anestésico local promoveu níveis plasmáticos de mepivacaína semelhantes aqueles obtidos pela adição de vasoconstritor. / Abstract: This study evaluated the tissue reaction induced by commercial solutions of 2% mepivacaine/1:100.000 epinephrine (MVC 2% EPI) and 3% mepivacaine (MVC 3%) and by the liposomal formulation of 2% mepivacaine (MVC LUV), with the "paw edema" test, in rats, observed 60, 120 and 180 minutes after the injections of these formulations. In addition, the tissue reaction in the oral mucosa induced by these formulations was analysed with histological techniques, after 6, 24 hours and 4 days. The plasmatic levels of these formulations were obtained after injection into oral mucosa by using mass spectrometry (HPLC- MS/MS) after 0, 15, 30, 45, 60, 120, 180, 240, 300, 360 and 420 of the injection. The "paw edema" test did not show statistically significant differences between the local anesthetic formulations tested in all periods (p>0.05). The histological evaluation of the oral mucosa, considering each time, showed lower intensity of inflammatory reaction with liposome formulation in comparison to MVC 2% EPI (p<0.05), but not with MVC 3% (p>0.05). The plasmatic levels obtained after MVC LUV injection did not showed statistically significant differences with the MVC 2% EPI levels considering all times (p>0.05). However, the MVC LUV plasmatic levels were significantly lower than the MVC 3% until 240 minutes (p<0.05). We concluded that the liposome formulation of mepivacaine induced less inflammatory reaction in oral mucosa than the commercial formulation with epinephrine. Besides, the encapsulation of mepivacaine into liposomes produced plasmatic levels of mepivacine similar to the vasoconstrictor addition. / Doutorado / Farmacologia, Anestesiologia e Terapeutica / Doutor em Odontologia

No description available.

The effects of representative anaesthetics on the polymorphic phase behavior of phospholipids, particularly phosphatidylethanolamine, is studied. The series of normal alcohols and alkanes destabilize the bilayer structure of hydrated phosphatidylethanolamine causing the lipid to adopt hexagonal (HII) structures at lower temperatures than control By exception, ethanol and butanol stabilize the bilayer configuration. Chloroform also initiates bilayer destabilization, producing this effect at clinically relevant concentrations of anaesthetic. The charged anaesthetics tested show the ability to stabilize the bilayer configuration of phosphatidylethanolamine to higher temperatures than controls this occurs in a single or mixed lipid system (ie. phosphatidylethanolamine / phosphatidylserine). In all cases the ability of an anaesthetic to alter the polymorphic phase behavior of phosphatidylethanolamine is a function of its individual potency. The observed effects of anaesthetic agent on lipid polymorphism can be interpreted in relation to the molecular shapes of the lipids and anaesthetics involved. / Medicine, Faculty of / Biochemistry and Molecular Biology, Department of / Graduate

No description available.