Submitted by (edna.saturno@ufrpe.br) on 2016-11-07T16:20:37Z No. of bitstreams: 1 Torquato Marques dos Santos Neto.pdf: 354695 bytes, checksum: 9dd614cfcb94578b80592c7d4d9d54a4 (MD5) / Made available in DSpace on 2016-11-07T16:20:37Z (GMT). No. of bitstreams: 1 Torquato Marques dos Santos Neto.pdf: 354695 bytes, checksum: 9dd614cfcb94578b80592c7d4d9d54a4 (MD5) Previous issue date: 2007-02-27 / The mastitis is the inflammatory process of infectious origin or not, that reaches the different structures of the mammary gland. It is characterized by alterations physical-chemistries and bacteriological of the milk and irreparable lesions in the mammary fabric. In goats explored for the production of milk, the mastitis is a serious problem, so much for increasing the costs of the production as for the risks to the public health. It was aimed at to evaluate clinicamente the animals, with relationship to the presence or not of mastites; To isolate and to identify the microorganisms found in the milk of the appraised animals; to determine the susceptibilitie of the isolated front to different antibiotics used in the mastitis treatment; to evaluate the antimicrobial activity of the propolis green in vitro; to determine the concentration minimum inibitory (CMI) of the green propolis; to evaluate the bactericidal effect and bacteriostatic of the green propolis. As experimental unit was established the use of the goat mammary half, for they be independent anatomicaly could present different infections. Initially 90goats were selected in nursing, presenting clinical mastite or subclinical, constituting a lot of 180 (hundred and eighty) affected mammary half for the disease. Samples of goat milk of some located properties were collected in the districts of Arcoverde, Brejo da Madre de Deus, Garanhuns, Jaboatão do Guararapes, Paudalho, Olinda, Pedra and São Lourenço, State of Pernanbuco; identified the microorganisms, isolated the colonies of Staphylococcus spp, the susceptibilitie was evaluated from the isolated front to different antibiotics used in the mastitis treatment (cefalotyne 30μg, gentamicyne 10μg, penicillyne G 10UI, sulfazotrim 25μg, tetraciclyne 30μg, neomicyne 30 μg, nitrofurantoyne 300 μg, ampicilyne 10μg, amoxicilyne 10μg, estreptomicyne 10μg, kanamicyne 30μg, lincomicyne 2μg and clorafenicol 30μg)and to the etanolics fractions and aqueous of the extracts of green propolis given in by the laboratory Necta-Farmacêutica®, for the diffusion methodology in disk.They were also certain the concentration minimum inibitory - CIM and bactericidal effect and bacteriostatic. The presence of clinical mastite was verified and it sub-practices medicine in the goat flock of the studied districts, and among the isolated ones starting from them sample of milk of animals with mastitis, form isolated colonies of Staphylococcus spp. In agreement with the evaluation done with the antibiotics used in the mastitis treatment the cefalotyne 30μg and the tetraciclyne 30μg they demonstrated larger efficiency front to these isolated with an index of 95.56% of sensibility. Being compared the values of the obtained inibition halus of the aqueous fractions and etanolic, it was verified that there was significant difference among them (p <0.01), suggesting that the formulation exercised activity antimicrobian in the studied cases. It was certain the concentration minimum inibitory of 100 mg/mL being obtained bactericidal effect in all the tested samples. It is suggested that obtained with the green propolis starting from the results, thiscan be used as an alternative treatment for goat mastitis, for having demonstrated efficiency with relationship to the bactericidal effect in the isolated ones tested. / A mastite é o processo inflamatório de origem infecciosa ou não, que acomete as diferentes estruturas da glândula mamária. Caracteriza-se por alterações físico-químicas e bacteriológicas do leite e lesões irreparáveis no tecido mamário. Em cabras exploradas para a produção de leite, a mastite é um grave problema, tanto por aumentar os custos da produção quanto pelos riscos à saúde pública. Objetivou-se com este estudo Isolar e identificar os microrganismos envolvidos nas mastites clínicas e subclínicas em cabras, determinar o perfil de sensibilidade dos Staphylococcus spp frente a diferentes antimicrobianos além de avaliar a atividade antimicrobiana determinar a concentração inibitória mínima (CIM) e avaliar o efeito bactericida e bacteriostático da própolis verde. Foram utilizadas 90 cabras em lactação, com mastite clínica ou subclínica, constituindo um total de 180 metades mamárias. As amostras de leite que foram submetidas ao exame microbiológico paraisolamento e identificação de Staphylococcus coagulase positiva e negativa que foram submetidos a testes de sensibilidade frente a 13 diferentes antimicrobianos e, às frações etanólica e aquosa do extrato de propólis verde cedidos pelo laboratório Necta-Farmacêutica®, utilizando a técnica de difusão em disco. Foi constatada a presença de mastite clínica e sub-clinica no rebanho caprino dos municípios estudados, e dentre os isolados a partir das amostras de leite de animais com mastite, form isoladas colônias de Staphylococcus spp. De acordo com a avaliação feita com os antibióticos utilizados no tratamento de mastites a cefalotina 30μg e a tetraciclina 30μg demonstraram maior eficácia frente a estes isolados com um índice de 95,56% de sensibilidade. Comparando-se os valores dos halos de inibição obtidos das frações aquosa e etanólica, constatou-se que houve diferença significativaentre eles (p < 0,01), sugerindo que a formulação exerceu atividade antimicrobiana nos casos estudados. Foi determinada a concentração inibitória mínima de 100 mg/mL obtendo-se efeito bactericida para todas as amostras testadas. Sugere-se, a partir dos resultados obtidos que a própolis verde, podeser utilizada como um tratamento alternativo para mastite caprina causada por Staphylococcus spp, por ter demonstrado eficácia in vitro quanto ao efeito bactericida nos isolados testados.