Typescript (photocopy). / Digitized by Kansas Correctional Industries

Typescript (photocopy). / Digitized by Kansas Correctional Industries

Call number: LD2668 .T4 1979 R65 / Master of Science

Call number: LD2668 .T4 1966 M648 / Master of Science

Submitted in complete fulfillment for the Degree of Master of Technology: Biotechnology, Durban University of Technology, 2010. / Xanthomonas campestris pv phaseoli produced an extracellular endoinulinase on various carbon sources. The highest inulinase production of 9.24 ± 0.03 IU ml¯¹by X. campestris pv. phaseoli was attained using an optimized medium comprising of 3% sucrose and 2.5% tryptone. Inulinase production in X. campestris pv. phaseoli was further enhanced through ethylmethanesulfonate mutagenesis. The resulting mutant, X. campestris pv. phaseoli KM 24 demonstrated enhanced inulinase production of 22.09 ± 0.03 IU ml¯¹after 24 h, which was 2.4 – fold higher than that of the wild type. Inulinase production by this mutant was scaled up in a 5 L fermenter yielding a final activity of 21.87 ± 0.03 IU ml¯¹with an inulinase/invertase (I/S) ratio of 2.6 after 18 h. Maximum volumetric (21 865 IU 1¯¹ h¯¹) and specific (119 025 IU g¯¹ h¯¹) productivities of inulinase were attained in a fermenter after 18h growth. Inulin hydrolysis by the crude inulinase and subsequent detection of mono- and oligosaccharides indicated the presence of an endoinulinase. The extracellular endoinulinase from the mutant KM 24 was purified to homogeneity by gel filtration chromatography and had a specific activity of 174.74U/mg. the optimum pH and temperature of the purified enzyme were found to be 6.0 and 50°C, respectively. The enzyme was stable up to 60°C, retaining over 60% activity for 30 min, but activity rapidly declined at temperatures above 60°C. The pure inulinase enzyme was also found to be stable between pH 6-9. The Lineweaver-Burk plots showed that the apparent Km and Vmax values of the inulinase for inulin were 1.15 mg/ml and 15µM/min, respectively. The Kcat value was found to be 0.145 min¯¹ with an enzyme catalytic efficiency of 0.126 mg¯¹.ml.min¯¹.This mutant demonstrated good potential for large scale production of inulinase and fructooligosaccharides. / National Research Foundation

Submitted in fulfilment for the requirement of a Degree of Doctor of Technology: Biotechnology, Durban University of Technology, 2010. / The aim of this study was to evaluate T. lanuginosus for the production of hemicellulases, its yield enhancement using mutagenesis and application of a selected xylanase on bagasse pupl to assess the improvement of pulp properties. The objectives were: To determine the localization of hemicellulases in T. lanuginosus strains, To develop high yielding strains of T. lanuginosus through mutagenensis, To investigate the synthesis of xylanase by T. lanuginosus MC134, To optimize the medium components and cultural conitions of T. lanuginosus MC134 strain, To study the influence of agitation and aeration on the production of xylanase by T. lanuginosus MC134 in a fermenter, To evaluate the bleach boosting abilities of T. lanuginosus xylanase on bagasse pulp, To evaluate simultaneous xylanase production and biobleaching potential of T. lanuginosus.

It is evident that human activity has an important impact on climate. Constantly increasing energy demand is one of the biggest causes of climate change. The fifth assessment report of the Inter-governmental panel on climate change states that decarbonisation of electricity generation is a key component of climate change mitigation. Increased awareness of this fact and escalating concerns around energy security has brought public attention to the energy industry, especially sustainable power generation systems. Future energy systems may need to include hydrogen as an energy carrier in order to achieve necessary levels of CO2 emission reductions, and overcome the challenges renewable energy systems present. Fuel cells could be a corner stone of future hydrogen inclusive energy solutions. New solutions like fuel cells have to compete with existing technologies and overcome the shortcomings of emerging technology. Though these shortcomings are well-recognised, fuel cells also have many advantages which makes continued research and development in the field highly worthwhile and viable. Key to their adoption is the identification of a niche market to utilise their advantages while overcoming their shortcomings with continuous research and development. This thesis aims to evaluate some of the stationary fuel cell applications and determine whether one could become the niche market as an entry point for fuel cells. This is achieved by economic evaluations of real and hypothetical applications. Results of the studies here imply that to decrease the total life cycle impacts of fuel cells to more acceptable levels, resource use in the manufacturing phase and recycling in decommissioning should be shown more attention. Results also present a picture showing that none of the applications investigated are economically feasible, given the current state of technology and energy prices. However, fuel cell-based combined cooling, heating and power systems for data centres show the potential to become the niche market that fuel cells need to grow. A further conclusion is that a broad market, longer stack lifetime, the possibility of selling electricity back to the grid and governmental subsidies are essential components of an environment in which fuel cells can permeate through the niche market to the mainstream markets. / <p>QC 20151210</p>

Smart mobile devices are becoming increasingly essential daily companions. Applications (apps) are the interface through which the consumer can leverage unique capabilities of smart mobile devices to interact with people, other devices and firms via the supporting mobile ecosystem. Smart mobile devices and apps are influencing how competition is defined and changing how firms do business by improving internal processes and increasing flexibility and convenience for customers. Mobile apps and devices enable users to move from a portable and mobile communication and computing environment to that of a ubiquitous communication and computing environment [u-space]. Discussion in terms of ‘mobile marketing’ is therefore too limiting, our understanding should be ‘ubiquitous marketing’. Six papers explore ubiquitous marketing further. The retail sector provides a contextual setting for paper one and finds that mobile marketing increases value for retailers and consumers. Integration of all retailer / consumer interfaces with mobile marketing to maximise exposure and connectivity between both parties is recommended. Paper two investigates the sources for mobile app ideas in companies and finds that apps developed externally or within the firm with some outside help, were perceived to be more effective. Apps that leverage the mobile devices unique features is central to the methodology proposed for developing an app in paper three. The next three papers examine the impact that mobile apps and devices have on business activities and customer relationships. Paper four finds increased operational efficiency in a Dental  Practice, while paper five identified the opportunity for increased firm-customer interaction in a medical context. Paper six determines that rather than five dimensions of SERVQUAL, financial service quality of apps consists of three dimensions: Reliability, personal and visibles; and that service success can be derived from providing less service. This thesis contributes to a fuller understanding of U-commerce theory. It advances understanding in how apps are making significant changes in how information technology is managed and controlled from an organisational perspective, and how these technology advances can influence consumer interaction. / <p>QC 20160516</p>

In this thesis, we study time-sensitive applications where it is important to minimize the completion time, i.e., time passing between receiving the instance and finishing the implementation of the solution. Different from the traditional approach, we are directly focusing on the minimization of the computation time as well as finding the optimal solution to the problem. The conventional approach to these conflicting objectives is generally to trade off one for the other. As an alternative, we propose a new approach called Computation-Implementation Parallelization (CIP), and develop methods to embed the computation time into the solution-implementation to minimize the total completion time. We implement our CIP approach and show its effectiveness on a type of TSP we call the TSP Race problem, where the goal is to minimize the time between receiving the instance and finishing the travel. We demonstrate a method for determining a priori when CIP will be effective. We also implement our CIP approach on Computation-Time Limited Capacitated Vehicle Routing (CTL-CVRP) problems, and show that it is possible to decrease the computation-only time while maintaining the solution quality. By this means, some of the computation time can be set free and used to improve the customer service either by delaying the order cutoff time or dispatching the trucks earlier. As a tangential study, we develop a new TSP tour length estimation model. Our model is distribution-free, and is shown to produce very accurate estimates on many different node dispersions.

Semantic annotations are crucial components in turning unstructured text into more meaningful and machine-understandable information. The acquisition of the mass of semantically-enriched information would allow applications that consume the information to gain wide benefits. At present there are a plethora of commercial and open-source services or tools for enriching documents with semantic annotations. Since there has been limited effort to compare such annotators, this study first surveys and compares them in multiple dimensions, including the techniques, the coverage and the quality of annotations. The overlap and the diversity in capabilities of annotators motivate the need of semantic annotation integration: middleware that produces a unified annotation with improved quality on top of diverse semantic annotators. The integration of semantic annotations leads to new challenges, both compared to usual data integration scenarios and to standard aggregation of machine learning tools. A set of approaches to these challenges are proposed that perform ontology-aware aggregation, adapting Maximum Entropy Markov models to the setting of ontology-based annotations. These approaches are further compared with the existing ontology-unaware supervised approaches, ontology-aware unsupervised methods and individual annotators, demonstrating their effectiveness by an overall improvement in all the testing scenarios. A middleware system – ROSeAnn and its corresponding APIs have been developed. In addition, this study also concerns the availability and usability of semantic-rich data. Thus the second focus of this thesis aims to allow users to query text annotated with different annotators by using both explicit and implicit knowledge. We describe our first step towards this, a query language and a prototype system – QUASAR that provides a uniform way to query multiple facets of annotated documents. We will show how integrating semantic annotations and utilizing external knowledge help in increasing the quality of query answers over annotated documents.