The work deals with the implementation of CRM solutions, it's representation in the reference model MBI, which is the main goal of the work. In the first part the market of CRM products is analyzed and the current trends in the industry are introduced. The second part is focused on the aforementioned extension of the reference model MBI based on the knowledge and the experience of professional literature in the implementation of CRM solutions. The extension is realized in the form of four tasks which generalize practices for implementing customer relationship management, further work is completed by factors that describe the practical procedures and instructions for working in CRM solutions. The application used is also defined in this work.

The aim of this thesis is the implementation and integration of solutions for Intelligent Warehouse Management into the ERP system, and its representation in the reference model of MBI. The work is composed of the individual chapters, of which the first chapters provides an extensive search to acquaint the reader with the basic characteristics of logistics, and logistics information technology support, which aims to improve KPI reporting and the level of SCM. The thesis also solves the characteristics and functionality of ERP and WMS systems and systems integration. The main part of the thesis describes the various stages of implementation and integration in the form of four tasks and their related factors representing general practices and procedures for the successful implementation of the system for Intelligent Warehouse Management.

The main objective of this work is to analyze the concepts of Business Intelligence to explore their implementation with MS Silverlight (RIA) to find out advantages and disadvantages of BI solutions using RIA and verified them on a case study of a fictitious bank. The work is divided into theoretical and practical part. The theoretical part is analyzing BI evolution and current trends. In the practical part the dashboard has been created for a fictitious bank management in MS Silverlight. The contribution of the author of this work lies mainly in the application of theoretical BI concepts in case study of a fictitious bank. There are elements of an MS Silverlight and processing techniques on the client that were used to show usability in BI. In the thesis was used Data Binding and MS Silverlight for predictive analysis in BI.

Diploma thesis deals with mobile applications for policyholders in the segment of general insurance. Aims of thesis are to create an analysis of existing applications on market in the Czech Republic, reveal their shortcomings, design a new application and then create it and make it available for policyholders of all insurers. The first chapter of thesis contains definition of basic terms that are used later in text. Analysis of existing applications is the content of second chapter. It is based on examination of functions of existing applications and the reviews from users and experts. Design of the new application is described in chapter three and is included as a runnable HTML prototype on the attached disk. Description of implemented application is included in the next part of the third chapter and application itself is available for download from Google Play and can also be found on the attached disk. The main benefits of thesis are the analysis of mobile insurance applications market, the new application available for all policyholders, and some recommendations resulting from re-search for companies intending to produce their own application.

The Internet today has a phenomenal reach---right into the homes of a vast audience worldwide. Some organisations (and individuals) see this medium as a good opportunity for extending the reach of their computer systems. One popular approach used for such endeavours is to run an application on a server, using web technology for displaying its user interface (UI) remotely. Developing such a web-based UI can be quite tedious---it is a concurrent, distributed program which has to run in a hostile environment. Furthermore, the platform on which it is implemented (the web) was not originally intended for such usage. A web framework is a collection of software components which provides its users with support for developing and executing web-based UIs. In part, web frameworks can be seen as being analogous to interpreters: given a specification of a UI using a specification technique dictated by the framework, server components of the framework can present the UI using web technology. Topics related to web frameworks are scarce in the academic literature, but abound in industry and open discussion forums. Similarly, the designers of web frameworks seldom found their work on existing theory in the literature. This study is an attempt to bridge this gap. It is focused on two aspects of web frameworks: the specification technique a framework mandates, and how such a specification can subsequently be used to present a UI via web technology. As part of this study, a survey was conducted of 80 open source web frameworks. Based on the survey, a partial overview of the domain of web frameworks is given, covering what is seen as being typically required of a web framework and covering specification techniques that are used by existing frameworks. Two taxonomies are proposed of the strategies web frameworks use for specifying two aspects of web UIs. Using the web as platform implies adherence to certain (intended) architectural constraints. Web framework designers often strain against these constraints. However, another point of view is to recognise that the success of the web platform is made possible precisely because of its intended architecture. (And the success of the web is surely the principal motivation for using it for remote UIs in the first place.) With the bias of this viewpoint, a specification technique is proposed for web-based UIs. This technique is based on the well-known formalism of statecharts, with semantics explicitly defined in terms of the intended architectural components and constraints of the web. The design of a web framework for presenting a UI so specified is also proposed (based on the theoretical background given, as well as two prototype implementations which have been developed). / Dissertation (MSc)--University of Pretoria, 2007. / Computer Science / unrestricted

Aplicações de captura e acesso exploram o paradigma de computação ubíqua --- que consiste em popular o ambiente com aplicações e dispositivos computacionais a fim de auxiliar transparentemente as pessoas na realização de suas atividades --- para dar apoio à captura automática de informação em experiências ``ao vivo' e à correspondente geração de documentos passíveis de armazenamento, recuperação, visualização e extensão ao longo do tempo. Devido à sua natureza distribuída, à heterogeneidade dos dispositivos computacionais envolvidos e à diversidade nas funcionalidades providas, essas aplicações são difíceis de se construir e requerem infra-estruturas e serviços de software que auxiliem o desenvolvedor nessa tarefa. Este trabalho investiga a construção e o uso de aplicações de captura e acesso por meio do desenvolvimento da xINCA, uma infra-estrutura estendida baseada em componentes de software reutilizáveis que englobam as funcionalidades recorrentes nessa classe de aplicações. A xINCA é uma extensão da infra-estrutura INCA --- uma infra-estrutura de baixo nível que provê abstrações de comunicação para aplicações de captura e acesso. Complementares, as infra-estruturas INCA e xINCA provêem um modelo simplificado para o desenvolvimento de aplicações de captura e acesso, considerando aspectos de projeto, implementação e reuso. Associada ao modelo de armazenamento do serviço StRES, a xINCA tem ainda papel na estruturação da informação capturada com o uso de XML e tecnologias correlatas. / Capture and access applications explore the ubiquitous computing paradigm --- that basically consists on populating the environment with computational devices and applications to transparently assist people on their activities --- to support the automatic capture of information on live experiences and generate corresponding documents that can be further stored, retrieved, visualized and extended. Due to their distributed nature, heterogeneity of involved devices and variety of provided functionalities, capture and access applications are hard to build and require software infrastructures and services that help the developer out on his task. This work investigates the building and use of capture and access applications through the development of xINCA, a component-based software infrastructure comprising the most common func-tio-na-li-ties present in such a class of applications. xINCA is an extension of INCA --- a low-level infrastructure that provides communication abstractions for capture and access applications. Complementary, INCA and xINCA provide a simplified model for the development of capture and access applications, concerning design, implementation and reuse aspects. Allied with the StRES storage model, xINCA also plays an important role on the structuring of captured information by using XML and corresponding technologies.

Induced pluripotent stem cells (iPSCs) have been recognised as a virtually unlimited source of stem cells that can be generated in a patient-specific manner. Due to these cells’ potential to give rise to all differentiated cell types of the human body, they have been widely used to derive differentiated cells for drug screening and disease modelling purposes. iPSCs also garner much interest as they can potentially serve as a source for cell replacement therapy. Towards the realisation of these biomedical applications, this thesis aims to address challenges that are associated with scale-up, safety and biofabrication. Firstly, the manufacture of a high number of human iPSCs (hiPSCs) will require standardised procedures for scale-up and the development of a flexible bioprocessing method, since standard adherent hiPSC culture exhibits limited scalability and is labour-intensive. While the quantity of cells that are required for cell therapy depends largely on the tissue and defect that these replacing cells are meant to correct, an estimate of 1 × 10^9 has been suggested to be sufficient for several indications, including myocardial infarction and islet replacement for diabetes. Here, the development of an integrated, microcarrier-free workflow to transition standard adherent hiPSC culture (6-well plates) to scalable stirred suspension culture in bioreactors (1 L working volume, 2.4 L maximum working volume) is presented. The two-phase bioprocess lasts 14 days and generates hiPSC aggregates measuring 198 ± 58 μm in diameter on the harvesting day, yielding close to 2 × 10^9 cells. hiPSCs can be maintained in stirred suspension for at least 7 weeks with weekly passaging, while exhibiting pluripotency-associated markers TRA-1-60, TRA-1-81, SSEA-4, OCT4, and SOX2. These cells retain their ability to differentiate into cells of all the three germ layers in vitro, exemplified by cells positive for AFP, SMA, or TUBB3. Additionally, they maintain a stable karyotype and continue to respond to specification cues, demonstrated by directed differentiation into beating cardiomyocyte-like cells. Therefore, the aim of manufacturing high hiPSC quantities was met using a state-of-the-art scalable suspension bioreactor platform. Secondly, multipotent stem cells such as induced neural stem cells (iNSCs) may represent a safer source of renewable cells compared to pluripotent stem cells. However, pre-conditioning of stem cells prior to transplantation is a delicate issue to ensure not only proper function in the host but also safety. Here, iNSCs which are normally maintained in the presence of factors such as hLIF, CHIR99021, and SB431542 were cultured in basal medium for distinct periods of time. This wash-out procedure results in lower proliferation while maintaining key neural stem cell marker PAX6, suggesting a transient pre-differentiated state. Such pre-treatment may aid transplantation studies to suppress tumourigenesis through transplanted cells, an approach that is being evaluated using a mouse model of experimental focal demyelination and autoimmune encephalomyelitis. Thirdly, biomedical applications of stem cells can benefit from recent advancements in biofabrication, where cells can be arranged in customisable topographical layouts. Employing a 3DDiscovery bioprinter, a bioink consisting of hiPSCs in gelatin-alginate was extruded into disc-shaped moulds or printed in a cross-hatch infill pattern and cross-linked with calcium ions. In both discs and printed patterns, hiPSCs recovered from these bioprints showed viability of around 70% even after 4 days of culture when loaded into gelatin-alginate solution in aggregate form. They maintained pluripotency-associated markers TRA-1-60 and SSEA-4 and continued to proliferate after re-plating. As further proof-of-principle, printed hiPSC 3D constructs were subjected to targeted neuronal differentiation, developing typical neurite outgrowth and resulting in a widespread network of cells throughout and within the topology of the printed matrix. Staining against TUBB3 confirmed neuronal identity of the differentiated cellular progeny. In conclusion, these data demonstrate that hiPSCs not only survive the 3D-printing process but were able to differentiate along the printed topology in cellular networks. / Induzierte pluripotente Stammzellen (iPSZ) stellen eine praktisch unbegrenzte Stammzellquelle dar, welche patientenspezifisch erzeugt werden kann. Da diese Zellen das Potenzial haben, alle differenzierten Zelltypen des menschlichen Körpers hervorzubringen, werden sie für die Herstellung differenzierter Zellen für Arzneimitteltests und für die Krankheitsmodellierung verwendet. Sie erfahren auch großes Interesse, weil sie als Zellquelle in der Zellersatztherapie Anwendung finden könnten. Die vorliegende Dissertation beschäftigt sich mit drei zentralen Herausforderungen, die im Rahmen der biomedizinischen Anwendung von iPSZ auftreten. Die Herstellung einer großen Zahl von humanen iPSZ (hiPSZ) erfordert die Entwicklung standardisierter Verfahren für die Skalierung, welche durch die Entwicklung einer flexiblen Bioprozessmethode realisiert werden kann. Bisher wird die Skalierbarkeit durch eine standardmäßig adhärente Zellkultur und den damit verbundenen hohen Arbeitsaufwand begrenzt. Die Menge an Zellen, die für die Zelltherapie benötigt wird, hängt stark vom Gewebetyp ab, welcher von den ersetzenden Zellen korrigiert werden soll. Berechnungen legen nahe, dass eine Anzahl 1 × 10^9 Zellen für eine Vielzahl von Indikationen ausreicht – einschließlich Myokardinfarkt und Inselzelltransplantation für Diabetes. Im Rahmen dieser Arbeit wurde ein integrierter Arbeitsablauf zur skalierbaren Zellsuspensionskultur von hiPSZ ohne Verwendung von microcarrier entwickelt, um die standardmäßig adhärente Kultur (6-Well-Platten) in Bioreaktoren (1 L Arbeitsvolumen, 2,4 L maximales Arbeitsvolumen) zu überführen. Der zweiphasige Produktionsprozess dauert 14 Tage und erzeugt hiPSZ-Aggregate mit einem finalen Durchmesser von 198 ± 58 μm, der annähernd 2 × 10^9 Zellen beinhaltet. hiPSZ können mindestens 7 Wochen lang in einer gerührten Zellsuspension bei wöchentlichem Passagieren gehalten werden, wobei sie Pluripotenz-assoziierte Marker wie TRA-1-60, TRA-1-81, SSEA-4, OCT4 und SOX2 beibehalten. Die Zellen behalten weiterhin ihre Fähigkeit, sich in vitro in Zellen mit AFP-, SMA- oder TUBB3-Immunoreaktivität und damit in Zellen aller drei Keimblätter zu differenzieren. Darüber hinaus halten sie einen stabilen Karyotyp aufrecht und reagieren auf gezielt eingesetzte externe Differenzierungsstimuli, wie durch eine gezielte Differenzierung in schlagende Kardiomyozyten-ähnliche Zellen demonstriert werden konnte. Somit wurde das Ziel, eine großen Anzahl hiPSCs herzustellen, mit einer hochmodernen, skalierbaren Suspensionsbioreaktorplattform erreicht. Multipotente Stammzellen wie induzierte neurale Stammzellen (iNSZ) gelten verglichen mit iPSZ als sicherere Zellquelle für Ersatztherapien. Die Vorkonditionierung von Stammzellen vor der Transplantation ist jedoch ein heikles Thema, da sowohl die einwandfreie Funktion im Wirtsgewebe als auch Sicherheit gewährleistet werden müssen. Im Rahmen dieser Arbeit wurden iNSZ, die normalerweise im Kulturmedium mit Faktoren wie hLIF, CHIR99021 und SB431542 gehalten werden, für eine definierte Zeitspanne in basalem Medium kultiviert. Die Vorbehandlung führt zu einer geringeren Proliferation, jedoch unter Erhalt der Expression des wichtigen neuralen Stammzellmarkers PAX6, was auf einen transienten vordifferenzierten Zustand hindeutet. Eine solche Vorbehandlung könnte bei zukünftigen Transplantationsstudien angewandt werden, um die Tumorentstehung durch transplantierte Zellen zu unterdrücken. Dieser Ansatz wird in Zukunft mit einem Mausmodell der experimentellen fokalen Demyelinisierung und der autoimmunen Enzephalomyelitis untersucht. Schließlich kann die Zellersatztherapie von den jüngsten Fortschritten in der Biofabrikation profitieren, bei der die Zellen durch das Drucken in anpassbare topographische Profile angeordnet werden können. Mit einem 3DDiscovery Biodrucker wurde eine Biotinte bestehend aus Gelatine-Alginat und hiPSZ in scheibenförmig extrudiert oder in einem Kreuzschraffurmuster gedruckt und mittels Kalziumionen-Zugabe vernetzt. Gedruckte hiPSZ zeigten auch nach 4 Tagen Kultivierung eine Lebensfähigkeit von etwa 70 % und weiterhin das Auftreten der Pluripotenz-assoziierten Marker TRA-1-60 und SSEA-4. Zudem konnten sie sich anschließend mit standardmäßig adhärenter Zellkultur weiter vermehren. Zudem konnte gezeigt werden, dass die gedruckten Konstrukte einer gezielten neuronalen Differenzierung unterzogen werden können, die zu einem typischen Neuritenauswuchs und zu einer weitreichenden interzellulären Vernetzung durch und innerhalb der Topologie der gedruckten Matrix führte. Die Färbung gegen TUBB3 bestätigte die neuronale Identität der differenzierten Zellen. Zusammenfassend zeigen diese Daten, dass bei Verwendung des in dieser Studie erarbeiteten Protokolls hiPSZ nicht nur den 3D-Druckprozess überleben, sondern auch entlang der gedruckten 3D Topologie in Netzwerke Neurone differenzieren können.

La creciente competitividad del mercado, genera una dificultad cada vez mayor en las organizaciones para alcanzar el éxito en sus proyectos. Tal hecho busca priorizar criterios económicos, tiempo, costo, calidad y alcance, ocasionando falta de controles que resultan en brechas de seguridad en la compañía. De esa forma se deja en segundo plano procedimientos de seguridad como por ejemplo el testeo de aplicaciones web. Estas poseen vulnerabilidades que podrían proporcionar los medios para que usuarios finales maliciosos violen mecanismos de protección de un sistema y obtengan acceso a información privada o recursos de la empresa. Los pronósticos referentes a la violación de datos indican que la industria de salud será el blanco más buscado para los ataques cibernéticos en 2017 ya que el alto valor de los registros de salud electrónicos (EHRs) llama cada vez más la atención de los cibercriminales. Dichos registros representan una fuente de ganancias mayor a la que si se accediera a información de tarjetas o cuentas bancarias. El presente proyecto propone un modelo de madurez de seguridad de aplicaciones web ante ciberataques para clínicas de nivel 2 bajo la norma técnica del MINSA, orientada a mostrar las debilidades de las aplicaciones web y las mejoras que se puedan realizar en aspectos de seguridad. El proyecto permitió la implementación de mejoras por parte de las empresas clientes en sus plataformas web mediante la recomendación propuesta por la guía de mejora luego de haber realizado el pentesting propuesto. / Bearing in mind that the projections made for the area of information security point to an increase in attacks on the health sector, added to the lack or little diffusion of security maturity models that allow organizations to know the status of their website in terms of security and that the existing models lack a post-evaluation monitoring, it is necessary to propose a model of security maturity of web applications against cyber-attacks, oriented to the health sector, which is simple to apply. The maturity model proposes to offer the user a portfolio of tools that asks them to apply tests and obtain their results, interpret them and place them at a level of maturity before cyberattacks, then proposing controls to improve the security of the web. This model will be based on the International Professional Practice Framework methodology and will include the main vulnerabilities published by the Open Web Application Security Project to propose attacks that identify the weakness of the evaluated web system, so that the client company has the possibility to reinforce its weaknesses. Guides will also be proposed to select strategies to improve critical points from a security perspective. Because of the validation, it was found that, of the 14 tests applied, five were approved, positioning the web at level 3 of maturity, which means that there are validations in the structure of the web; however, they are partial or inefficient. / Tesis

Human beings by nature have a predisposition towards learning and the exploration of the natural world. We are intrinsically intellectual and social beings knitted with adaptive cognitive architectures. As Foot (2014) succinctly sums it up: “humans act collectively, learn by doing, and communicate in and via their actions” and they “… make, employ, and adapt tools of all kinds to learn and communicate” and “community is central to the process of making and interpreting meaning—and thus to all forms of learning, communicating, and acting” (p.3). Education remains pivotal in the transmission of social values including language, knowledge, science, technology, and an avalanche of others. Indeed, Science, Technology, Engineering, and Mathematics (STEM) have been significant to the advancement of social cultures transcending every epoch to contemporary times. As Jasanoff (2004) poignantly observed, “the ways in which we know and represent the world (both nature and society) are inseparable from the ways in which we choose to live in it. […] Scientific knowledge [..] both embeds and is embedded in social practices, identities, norms, conventions, discourses, instruments, and institutions” (p.2-3). In essence, science remains both a tacit and an explicit cultural activity through which human beings explore their own world, discover nature, create knowledge and technology towards their progress and existence. This has been possible through the interaction and applications of artifacts, tools, and technologies within the purviews of their environments. The applications of technologies are found across almost every luster of organizational learning especially teacher education, STEM, architecture, manufacturing, and a flurry of others. Thus, human evolution and development are inexplicably linked with education either formally or informally. The 21st century has however seen a surge in the use of artificial intelligence (AI) and digital technologies in education. The proliferation of artificial intelligence and associated technologies are creating new overtures of digital multiculturalism with distinct worldviews of significance to education. For example, learners are demonstrating digital literacy skills and are knowledgeable about AI technologies across every specter of their lives (Bennett et al., 2008). It is also opening new artesian well-springs of educational opportunities and pedagogical applications. This includes mapping new methodological pathways, content creation and curriculum design, career preparations and indeed a seemingly new paradigm shift in teaching STEM. There is growing scholarly evidence about the use and diffusion of these technologies in K-12 and higher education (Bonk & Graham, 2012; Hew & Brush, 2007; Langer, 2018; Mishra & Koehler, 2006). Some of these include the Sphero robots, Micro Bit, Jill Watson, BrickPi3 Classroom kit, Engino STEM Mechanic, Lego Education WeDo Core Set and Spike. Both educators and learners are using these in STEM programs as well as other education related activities. Just as human activities and interactions with artifacts and tools shaped and redefined the scientific-technological feat of previous generations, so the contemporary digital technological era seems to be on a similar trajectory. However, there is sparsity of empirical scholarship on the pedagogical prospects and effectiveness of artificial intelligence in STEM classrooms. Also, it should be noted that scholarship on how AI impacts pedagogical content knowledge of STEM educators and how learners perceive these technologies are just emerging. In addition, the recent COVID-19 pandemic (Ghandhi et al., 2020; Rasmussen et al., 2020) has unexpectedly created a renewed synergy towards the applications of digital technologies in teaching STEM. In the context of this force majeure (COVID-19), the traditional brick and mortar educational spaces metamorphosed into digital spaces with the applications of many artificial intelligent technologies and resources in the arena of education. This doctoral dissertation study examined these enigmas including how educators use these technologies in STEM classrooms. The study is informed by activity theory or cultural-historical activity theory (Engeström et al., 2007; Hasan et al., 2014; Krinski & Barker, 2009; Oers, 2010; Vygotsky,1987). The study participants will be selected from educators currently integrating artificial intelligent systems and digital technologies in their respective STEM classrooms. Pre-data survey inquiry has shown that many educators were incorporating some forms of AIS into their STEM classrooms. In view of these, I have explored Sphero educational robots to interrogate the research topic. The Sphero Edu described as a “…STEAM-based toolset that weaves hardware, software, and community engagement to promote 21st century skills. While these skills are absolutely crucial, our edu program goes beyond code by nurturing students’ creativity and ingenuity like no other education program can” (Sphero, April 2020). The Sphero robots also have features and applications for designing and teaching STEM topics such as nature, space science, geometry, and other activities of pedagogical significance. Users could also design and write advanced engineering programs in JavaScript during STEM educational activities formally and outside of the classrooms. In essence, educators and students can learn designing, programming, engineering, mathematics, computational thinking, and hands-on skills reflective of the 21st century. In brief, the dissertation study research has explored artificial intelligence and emerging technologies and how these could transform and advance teaching and learning of STEM hence the research topic: Exploring the use of Artificial Intelligent Systems in STEM Classrooms. Methodologically, this is a qualitative study through the theoretical frameworks of activity theory as applicable to STEM education. The main research questions are: 1) Given that artificial intelligent systems and digital technologies have been applied in STEM educational domains (content, pedagogy, student learning, assessment). How does the application of AIS and digital technologies impact pedagogy in STEM educational activities? 2) Given that digital technology is transforming contemporary society in every facet. How/What does AIS tell us about how digital technology impacts STEM pedagogy? Data was collected from the study participants, archival sources, and others for analyses. It is hoped that the findings will inform and address theories of learning and teaching, policy and praxis in science education, teacher preparatory and professional development programs as it relates to STEM classrooms

<p>In the first chapter, we illustrate a big picture of the printing systems and the concentration of this dissertation. </p><p><br></p><p>In the second chapter, we present a tone-dependent fast error diffusion algorithm for color images, in which the quantizer is based on a simulated linearized printer space and the filter weight function depends on the ratio of the luminance of the current pixel to the maximum luminance value. The pixels are processed according to a serpentine scan instead of the classic raster scan. We compare the results of our algorithm to those achieved using</p> <p>the fixed Floyd-Steinberg weights and processing the image according to a raster scan ordering. In the third chapter, we first design a defect generator to generate the synthetic abnormal</p> <p>printer sounds, and then develop or explore three features for sound-based anomaly detection. In the fourth chapter, we explore six classifiers as our anomaly detection models, and explore or develop six augmentation methods to see whether or not an augmented dataset can improve the model performance. In the fifth chapter, we illustrate the data arrangement and the evaluation methods. Finally, we show the evaluation results based on</p> <p>different inputs, different features, and different classifiers.</p> <p><br></p><p>In the last chapter, we summarize the contributions of this dissertation.</p>