Ctenocephalides felis est une espèce de puce cosmopolite parasitant majoritairement les carnivores domestiques. Elle est vectrice de nombreux agents pathogènes zoonotiques dont les bactéries du genre Bartonella, bactéries intracellulaires facultatives. La compétence vectorielle de cette puce a été investiguée pour B. henselae, B. quintana, B. clarridgeiae, B. tribocorum et B. birtlesii. Dans ces conditions expérimentales, utilisant un système de gorgement sur membrane, ces espèces ont persisté pendant les trois jours d'une première étude, et pour B. henselae durant les 13 jours de survie des puces, dans une seconde étude. Les cinq espèces de bartonelles ont été retouvées dans les fèces. Pour ces cinq espèces, nos résultats montrent une absence de transmission verticale transovarienne chez la puce et suggèrent une possibilité de contamination horizontale. Nous proposons enfin un protocole original d'évaluation de l'efficacité d'un traitement antiparasitaire chez le chat, pour prévenir sa contamination par Bartonella spp. / Ctenocephalides felis is a cosmopolitan flea species mainly parasitizing pets, transmitting several pathogens of veterinary and zoonotic importance including the facultative intracellular bacteria of the genus Bartonella. The vector competence of this flea was investigated for B. henselae, B. quintana, B. clarridgeiae, B. tribocorum and B. birtlesii, using an artificial feeding system. In these experimental conditions, these bartonellae proved to persist for three days, in a first study, while B. henselae persisted for the 13 days of its life span, in a second study. All five bartonellae were excreted in the flea's faeces. On the whole, these five species were not transmitted transovarially in the fleas, though horizontal transmission was suggested. Furthermore, we propose an original protocol allowing the evaluation of the efficacy of ectoparasiticidal products against Bartonella spp. infection in cats.

Submitted by Johnny Rodrigues (johnnyrodrigues@ufcg.edu.br) on 2018-05-15T17:57:54Z No. of bitstreams: 1 JOSÉ TOMAZ DE AQUINO - DISSERTAÇÃO PPGSA PROFISSIONAL 2013..pdf: 1522630 bytes, checksum: 67ae7d0f19a1c3206eca380aa90b19d0 (MD5) / Made available in DSpace on 2018-05-15T17:57:54Z (GMT). No. of bitstreams: 1 JOSÉ TOMAZ DE AQUINO - DISSERTAÇÃO PPGSA PROFISSIONAL 2013..pdf: 1522630 bytes, checksum: 67ae7d0f19a1c3206eca380aa90b19d0 (MD5) Previous issue date: 2013-07 / A africanização foi responsável por muitas mudanças no comportamento das abelhas européias, que já habitavam no Brasil há algum tempo. Objetivou-se analisar a agressividade de colônias apis mellífera no semiárido paraibano. A pesquisa foi realizada no município de Cajazeiras, PB em um apiário no distrito de Catolé. O município está situado na região semiárida do Nordeste brasileiro, no estado da Paraíba. O apiário é composto por 60 colmeias com enxames de abelhas africanizadas (Apis mellifera) e está instalado no local desde 1983. Todas as caixas foram enumeradas na parte frontal do ninho para facilitar a identificação. Foram selecionadas 20 colmeias, dessas 20 foram escolhidas 10, nas quais se forneceu a alimentação artificial, as outras 10 colmeias não foram alimentadas artificialmente. Os testes foram realizados em três momentos que compreende os meses de agosto, outubro e dezembro de 2012. Os parâmetros analisados foram Tempo para ocorrer a primeira ferroada em um retalho de camurça, número de ferrões deixados no retalho de camurça preto, número de abelhas que atacaram e foram presas no recipiente, distância que as abelhas perseguem o observador e tempo para a calmaria das abelhas. Para o tempo de ocorrência para a primeira ferroada, a colônia 24 alimentada levou um menor tempo para atacar, indicando ser mais agressiva. Para as colônias não alimentadas a colônia 26 foi a que apresentou um maior numero de abelhas atacando nos primeiros 60s. Para as colônias alimentadas não houve diferença entre a colônia 40 e a 46, sendo a colônia 46 a que teve um maior número de abelhas que atacaram. Para a distancia percorrida a colônia 14 não alimentada foi a que perseguiu o observador a uma distancia maior com uma media de 436,8m, já para as colônias alimentadas a colônia 49 foi a que perseguiu a uma maior distancia chegando a 488,3m. Os estudos de defensividade demonstraram que existem diferenças em relação ao nível de defensividade das colônias de apis melífera estudadas, sendo necessário mais estudo para melhor analisar a defensividade de abelhas no semiárido paraibano. / The Africanization was responsible for many changes in the behavior of European honey bees, which inhabited in Brazil for some time. This study aimed to analyze the aggressiveness apis mellifera colonies in semiarid Paraiba. The survey was conducted in the municipality of Cajazeiras PB in an apiary District of Catole. The municipality is located in the semiarid region of northeastern Brazil, in the state of Paraiba. The apiary is composed of 60 hives with swarms of Africanized honey bees (Apis mellifera) and is installed in that place since 1983. All the boxes were listed in front of the nest to facilitate identification. 20 colonies were selected, 10 of these 20 were selected, which are provided in the artificial feeding, the other 10 colonies were not artificially fed. The tests were conducted in three stages comprising the months of August, October and December 2012. The parameters analyzed were to occur the first time in sting retail suede, number of stings left in black suede flap, the number of bees that attacked and were arrested in the container, the bees chase away the observer and time for the lull bees. For the time of occurrence for the first sting, the colony fed 24 took a little time to attack, indicating that more aggressive. For colonies not fed the colony 26 showed the greater number of bees attacking in the first 60s. For colonies fed no difference between the colony 40 and 46, 46 being the colony that had a greater number of bees attacked. Distance traveled to the colony 14 was not fed that chased the observer at a distance greater with an average of 436,8 m , while for the 49 colonies fed the colony that was pursued to a greater distance reaching 488,3 m . Defensiveness studies demonstrated that differences exist in the level of defensiveness of apis mellifera colonies studied, more study is needed to better analyze the defensiveness of bees in the semiarid Paraiba.

Les insectes sont soumis aux fluctuations thermiques de leur environnement mais disposent d’un panel varié de réponses comportementales, physiologiques et biochimiques pour en minimiser les effets délétères et maintenir leur intégrité physiologique. Ainsi certaines espèces régulent activement leur température interne indépendamment de la température de l’environnement. Si ces insectes peuvent s’affranchir des contraintes thermiques imposées par leur environnement, ceux qui se nourrissent du sang chaud d’hôtes vertébrés endothermes n’ont pas d’autres choix que de se confronter à une situation de stress thermique à chaque prise alimentaire. Le principal objectif de ce travail de thèse est de comprendre comment des insectes hématophages, employant des stratégies alimentaires différentes, gèrent le stress thermique associé au flux massif de chaleur engendré par l’ingestion du repas de sang. Nos résultats montrent que ces insectes ont su s’adapter en développant différentes stratégies de thermorégulation. / Insects are submitted to thermal fluctuations of their environment and have developed a wide ranged panel of behavioral, physiological and biochemical responses, to minimize the subsequent deleterious effects and maintain their physiological integrity. Some species actively regulate their internal temperature independently of the temperature of the environment. If these insects can overcome the constraints imposed by their thermal environment, those that feed on warm-blooded vertebrate hosts have no choice but to confront a situation of thermal stress at each feeding event. The main objective of this work is to understand how bloodsucking insects manage heat stress associated with the massive flow of heat generated by the ingestion of the blood meal. Our results show these insects have developed different strategies of thermoregulation to protect themselves from overheating.