Submitted by Ricardo Cedraz Duque Moliterno (ricardo.moliterno@uefs.br) on 2015-10-07T21:05:32Z No. of bitstreams: 1 68-maiane-neve-dissertacao-completa-30-06-2015.pdf: 2173788 bytes, checksum: 01667558c2c58926c53a0a2c86f9ff1d (MD5) / Made available in DSpace on 2015-10-07T21:05:32Z (GMT). No. of bitstreams: 1 68-maiane-neve-dissertacao-completa-30-06-2015.pdf: 2173788 bytes, checksum: 01667558c2c58926c53a0a2c86f9ff1d (MD5) Previous issue date: 2015-06-30 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / Mimosa tenuiflora (Willd.) Poiret (jurema preta) is a species in the Fabaceae family, native to the Caatinga, found throughout the Brazilian Northeast. In folk medicine, the shells of the stem and root of jurema preta are used in treatment of several diseases, as burns and inflammations, besides being maid traditionally as drink in indigenous rituals. Regarding to the root bark, there are few studies described in the literature about its phytochemical profile and biological properties. In this way, the present study has proposed perform a phytochemical study of the extracts from the root bark of Mimosa tenuiflora. For this, it was performed the fractionation of chloroformic extract from the column chromatography (CC), and the structural determinations of the isolated compounds were carried out by technique of nuclear magnetic resonance (NMR) of 1H and 13C, besides the identification and quantification of phenolic substances in the chloroformic extracts and the ethyl acetate from the high-efficiency liquid chromatography with diode arrangement detector (HPLC-DAD) and consequent validation of applied analytical method. Were also carried out assessment of anticholinesterasic activity of the extracts by the method of Ellman and quantification of the total flavonoid content of the crude extract through spectrophotometric method using as reactant aluminum chloride (AlCl3) and standard curve with quercetin. As a result, were obtained from the chloroformic extract a mixture of the ?-sitosterol and stigmasterol steroids and the betulinic acid triterpene. From of the HPLC-DAD were identified polyphenols such as catechin, epicatechin and procyanidin B2 beyond the gallic acid in the ethyl acetate extract and procyanidin B2, in the chloroformic extract of this species. With the validation of the analytical method, this proved to be reliable and suitable for the quantification of the substance catechin and epicatechin in the ethyl acetate extract, according to criteria recommended by the National Health Surveillance Agency ? NHSA. Regarding the anticholinesterasic activity, among the tested extracts of black jurema, the gross and the ethyl acetate were the ones who showed greater inhibition capacity on the enzyme acetylcholinesterase (AChE) in relation to other, presenting percentages of 89% + 0,31 and 93,8% + 0,4, respectively, what classifies this species as a potent inhibitor. The total flavonoids content quantified in the crude extract of Mimosa tenuiflora was of 1,386g/100g extract. Thus, this work indicates that the species Mimosa tenuiflora is a promising source of bioactive substances of chemical-medicinal interest, besides being a strong natural candidate that can aid in the treatment of Alzheimer's disease. It is important to mention that the present study provides an unprecedented contribution about the chemical composition of root bark of black jurema, since to date no records were found in the literature on phenolic constituents of this part of the plant. / Mimosa tenuiflora (Willd.) Poiret (jurema preta) ? uma esp?cie da fam?lia Fabaceae nativa da Caatinga, encontrada largamente em todo nordeste brasileiro. Na medicina popular, as cascas do caule e da raiz da jurema preta s?o utilizadas no tratamento de diversas enfermidades, como queimaduras e inflama??es, al?m de ser empregada tradicionalmente como bebida em rituais ind?genas. Com rela??o ? casca da raiz, h? poucos estudos descritos na literatura sobre seu perfil qu?mico e propriedades biol?gicas. Desta forma, o presente trabalho prop?s realizar um estudo fitoqu?mico dos extratos da casca da raiz da Mimosa tenuiflora. Para isso, foi realizado o fracionamento do extrato clorof?rmico a partir da cromatografia em coluna (CC), e as determina??es estruturais das subst?ncias isoladas foram realizadas atrav?s da t?cnica de resson?ncia magn?tica nuclear (RMN) de 1H e 13C, al?m da identifica??o e quantifica??o de subst?ncias fen?licas nos extratos clorof?rmico e acetato de etila a partir da cromatografia a l?quidos de alta efici?ncia com detector de arranjo de diodos (CLAE-DAD) e consequente valida??o do m?todo anal?tico aplicado. Tamb?m foram realizadas avalia??o da atividade anticolinester?sica dos extratos pelo m?todo de Ellman e quantifica??o do teor de flavonoides totais do extrato bruto atrav?s de m?todo espectrofotom?trico utilizando-se como reagente cloreto de alum?nio (AlCl3) e curva padr?o com quercetina. Como resultado, foram obtidos do extrato clorof?rmico uma mistura dos esteroides ?-sitosterol e estigmasterol e o triterpeno ?cido betul?nico. A partir da CLAE-DAD foram identificados polifen?is como a catequina, epicatequina e procianidina B2 al?m do ?cido g?lico no extrato acetato de etila e procianidina B2, no extrato clorof?rmico desta esp?cie. Com a valida??o do m?todo anal?tico, este se mostrou confi?vel e adequado para a quantifica??o das subst?ncias catequina e epicatequina no extrato acetato de etila, de acordo com crit?rios preconizados pela Ag?ncia Nacional de Vigil?ncia Sanit?ria - ANVISA. Com rela??o ? atividade anticolinester?sica, entre os extratos testados da jurema preta, o bruto e o de acetato de etila foram os que demonstraram maior capacidade de inibi??o sobre a enzima acetilcolinesterase (AChE) com rela??o aos demais, apresentando percentuais de 89% + 0,31 e 93,8% + 0,4, respectivamente, o que classifica esta esp?cie como potente inibidor. O teor de flavonoides totais quantificado no extrato bruto da Mimosa tenuiflora foi de 1,386g/100g extrato. Sendo assim, este trabalho indica que a esp?cie Mimosa tenuiflora ? uma fonte promissora de subst?ncias bioativas de interesse qu?mico-medicinal, al?m de ser uma forte candidata natural que pode auxiliar no tratamento do mal de Alzheimer. ? importante mencionar que o presente estudo fornece uma contribui??o in?dita acerca da composi??o qu?mica da casca da raiz da jurema preta, uma vez que at? o momento n?o foram encontrados registros na literatura sobre constituintes fen?licos desta parte da planta.

Submitted by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-02-19T13:03:27Z No. of bitstreams: 5 abraao.pdf: 3789153 bytes, checksum: 0d883e26dd234b7e0859f99b195f74c4 (MD5) license_url: 52 bytes, checksum: 3d480ae6c91e310daba2020f8787d6f9 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) license.txt: 2110 bytes, checksum: b4c884761e4c6c296ab2179d378436d4 (MD5) / Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-02-19T13:44:19Z (GMT) No. of bitstreams: 5 abraao.pdf: 3789153 bytes, checksum: 0d883e26dd234b7e0859f99b195f74c4 (MD5) license_url: 52 bytes, checksum: 3d480ae6c91e310daba2020f8787d6f9 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) license.txt: 2110 bytes, checksum: b4c884761e4c6c296ab2179d378436d4 (MD5) / Made available in DSpace on 2015-02-19T13:44:19Z (GMT). No. of bitstreams: 5 abraao.pdf: 3789153 bytes, checksum: 0d883e26dd234b7e0859f99b195f74c4 (MD5) license_url: 52 bytes, checksum: 3d480ae6c91e310daba2020f8787d6f9 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) license.txt: 2110 bytes, checksum: b4c884761e4c6c296ab2179d378436d4 (MD5) Previous issue date: 2013-02-19 / O trabalho apresentado nesta disserta??o descreve o estudo fitoqu?mico e de atividade biol?gica das folhas e caules e flores de Plectranthus neochilus Schltr., esp?cie pertencente ? fam?lia Lamiaceae. A planta ? uma erva arom?tica conhecida popularmente no Brasil como ?boldo? ou ?boldo-gamb??, e assim como as esp?cies P. barbatus Andrews (?boldo-brasileiro") e P. ornatus Codd. ("boldinho"), tem o ch? de suas folhas utilizadas na medicina tradicional no tratamento de insufici?ncia hep?tica e dispepsia, em substitui??o a Peumus boldus Molina (?boldo-do-chile?). O estudo fitoqu?mico do extrato hex?nico das folhas e caules de P. neochilus forneceu dois triterpenos: ?-amirina (PN1) e friedelina (PN2). PN1 foi isolada como um ?ster de ?cido graxo ligado na posi??o C-3. Deste extrato foi obtida tamb?m a mistura dos ester?ides sitosterol e estigmasterol (PN3). O estudo fitoqu?mico conduzido com o extrato em acetato de etila das folhas e caules forneceu a flavona metoxilada nas posi??es C-6 e C-7, a cirsimartina (PN4). Todos os compostos obtidos foram descritos pela primeira vez na esp?cie. A elucida??o estrutural dos compostos isolados, baseou-se na an?lise espectrosc?pica de RMN 1D, CLAE, ESI e por compara??o com dados encontrados na literatura. O extrato metan?lico das folhas e caules de P. neochilus mostrou significativa atividade antioxidante quando avaliado pelo m?todo de DPPH, seguido pelo extrato em acetato de etila e hex?nico. Foi avaliado tamb?m o extrato metan?lico das flores da esp?cie, que apresentou atividade inferior aquele observado para o extrato metan?lico das folhas e caule, sendo estas atividades confirmadas pelo conte?do fen?lico dos extratos. A atividade anticolinester?sica dos extratos das folhas e caules e das flores de P. neochilus foi avaliada, sendo observada a inibi??o da enzima acetilcolinesterase para todos os extratos pesquisados. / Disserta??o (Mestrado) ? Programa de P?s-Gradua??o em Qu?mica, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2011. / ABSTRACT The present work describes the phytochemical and pharmacological investigation on extracts of the leaves and stems and flowers of the species Plectranthus neochilus Schltr. belonged to the family Lamiaceae. The plant is aromatic herb popularly known in Brazil as ?boldo? or ?boldo-gamb??, and as the species P. barbatus Andrews (?boldo-brasileiro") e P. ornatus Codd. ("boldinho"), according to ethnopharmacological survey, is often used in the treatment of hepatic insufficiency and dyspepsia, replacing the Peumus boldus Molina (?boldo-do-chile?). The phytochemical investigation of the hexanic extract of the P. neochilus leaves and stems yielded two triterpenes: ?-amyrin (PN1) e friedelin (PN2). The compound ?-amirin was isolated as a fatty acid ester in position C-3. In this extract was also obtained the mixture of the steroids sitosterol and stigmasterol (PN3). The phytochemical study conducted with the ethyl acetate extract of the leaves and stems gave the methoxy flavone at positions C-6 and C-7, the cirsimartin (PN4). All compounds obtained were first described in the species. Structural elucidation of isolated compounds was based on spectroscopic analysis of 1D NMR, HPLC, ESY and by comparison with data found in literature. The methanol extract of leaves and stems of P. neochilus showed significant antioxidant activity when evaluated by the DPPH method, followed by the extract in ethyl acetate and hexane. We also evaluate the methanol extract of flowers of the species that showed activity lower than that observed for the methanolic extract from leaves and stems, and these activities confirmed for the content of phenolic extracts. The anticholinesterase activity of extracts of leaves and stems and flowers of P. neochilus was evaluated and found to inhibit the enzyme acetylcholinesterase to all extracts studied.