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A policy analysis of the prevention of human infection of Avian Flu in Hong Kong /Ho, Chi-keung, Albert. January 2005 (has links)
Thesis (M.P.A.)--University of Hong Kong, 2005.
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Comparative pathogenesis of virulent and avirulent avian influenza viruses in turkeysResende, Mauricio. January 1980 (has links)
Thesis (Ph. D.)--University of Wisconsin--Madison, 1980. / Typescript. Vita. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references (leaves 119-130).
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Antigenic Characterization of H3 And H7 Avian Influenza A Virus from Migratory Waterfowl in North AmericaBailey, Laura Elizabeth 09 May 2015 (has links)
Avian influenza A viruses pose threats to public health and agriculture stability. Historically, each of the four influenza A viruses responsible for pandemics in the last century contained at least one gene segment of avian origin. Migratory waterfowl are natural reservoirs of influenza A viruses and are capable of widespread dissemination. In this study, we aim to characterize the antigenic profiles of H3 and H7 avian influenza A viruses currently circulating in migratory waterfowl within North America. By understanding the antigenic diversity of these subtypes, we can understand the natural history of influenza evolution and develop potential disease preventive strategies.
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Genetic adaptation of an avian influenza A virus to swine cellsBourret, Vincent Jacques Richard January 2014 (has links)
No description available.
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Optimization of detection of avian influenza virus in formalin fixed tissues by immunohistochemical methodsWong, Pik-wa, Linda. January 2009 (has links)
Thesis (M. Med. Sc.)--University of Hong Kong, 2009. / Includes bibliographical references (p. 63-70).
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Molecular characterization of H3N2 influenza viruses isolated from ducks at a single Hong Kong farm : their diversity and evolution in natural reservoirs /Leung, On-cheung. January 2002 (has links)
Thesis (M. Phil.)--University of Hong Kong, 2002. / Includes bibliographical references (leaves 116-132).
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Development of recombinant adeno-associated virus delivering short-hairpin RNAs to inhibit the replication of influenza A virusesZhang, Gui, 张桂 January 2011 (has links)
published_or_final_version / Microbiology / Doctoral / Doctor of Philosophy
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A mutation in avian influenza H5 hemagglutinin with efficient packaging into lentiviral backbone and its implications on receptorbindingLam, Yuen-man, 林婉雯 January 2011 (has links)
Because diagnostic tests for highly pathogenic avian influenza (HPAI) viruses require
the use of replication-competent viruses in a biosafety level 3 containment, numerous
studies have looked at ways to develop alternative tests. Lentiviral particles
pseudotyped with H5 hemagglutinin (HA), the surface glycoprotein of influenza virus,
have been described as useful and safe tools for research and serological surveillance
on the HPAI viruses. However, not all H5 HA give rise to efficient H5 pseudotyped
lentiviral particles (H5pp) production. HA from A/Cambodia/408008/05 H5N1
(H5Cam) and HA from A/Anhui/1/05 H5N1 (H5Anh) exhibit a dramatic difference in
their ability to pseudotype lentiviral particles. H5Cam gives the highest H5pp
production among all HAs tested, whereas the lowest has been observed with H5Anh.
The objective of this study was to investigate the molecular determinants that govern
efficient H5pp production.
Based on the amino acid differences between H5Cam and H5Anh, H5Anh mutants
were generated by site-directed mutagenesis. Strikingly, a single amino acid change,
A134V, in the 130-loop receptor-binding domain of HA, significantly increased H5pp
production with H5Anh. The finding that valine 134 is crucial for H5pp production was
confirmed by reciprocal H5Cam and H5Anh mutants, which displayed either a
dramatic decrease or increase in H5pp production, respectively.
Influenza virus and H5pp bud at the plasma membrane, therefore changes in HA cell
surface expression could affect the production of H5pp. Thus, cell surface expressions
of H5Cam and H5Anh were compared by flow cytometry. Intriguingly, H5Cam
displayed a higher plasma membrane expression than H5Anh, suggesting that transport
is important for H5pp production. Introduction of V134A mutation in H5Cam reduced
its surface expression to that of H5Anh; by contrast, H5Anh mutant harboring A134V
mutation largely restored its expression.
Next the effect of A134V mutation on the binding of HA to sialic acid receptors was
investigated. A cell-based Enzyme-linked Immunosorbent Assay was developed to
measure binding of wild-type and mutated HA. Soluble recombinant proteins were
produced by mammalian cells stably transfected with HA gene ectodomain and were
mostly trimeric as indicated by discontinuous native gel electrophoresis. Interestingly,
H5Anh proteins exhibited a stronger binding to MDCK cells than H5Cam proteins, and
introduction of A134V mutation in H5Anh proteins reduced the binding. By contrast,
as predicted, the reciprocal V134A mutation induced a major increase in binding to
cellular receptors. It is likely that stronger binding of H5Anh to sialic acids could
hinder the release of H5pp. Consistent with this notion, the ability of H5Anh to
generate H5pp was significantly increased in a sialylation deficient Lec2 cell, a CHO
mutant cell line.
In conclusion, H5Cam allows efficient H5pp production whereas H5Anh does not.
With several lines of evidence, it is likely that the behavior of H5Anh can be explained
by a stronger binding to sialic acid receptors that is dependent on a single amino acid
residue at position 134. Since A134V is a naturally occurring mutation observed
occasionally in human host, these results may have implications for the understanding
of human host adaptations of H5N1 viruses. / published_or_final_version / Biochemistry / Master / Master of Philosophy
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The genesis and development of H5N1 influenza virus in poultry in ChinaDuan, Lian, 段炼 January 2011 (has links)
published_or_final_version / Microbiology / Doctoral / Doctor of Philosophy
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Ecology, epidemiology and immunology of avian influenza virusLeung, Yin-hung, Connie., 梁彥虹. January 2011 (has links)
published_or_final_version / Microbiology / Doctoral / Doctor of Philosophy
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