This present study compares the effect on intraperitoneal injections of the following drugs on a conditioned approach-avoidance response in mice. These drugs were epinephrine and Normotensin, an epinephrine neutralizing hormone.

It was the purpose of the present study to compare the effect of intraperitoneal injections of the following drugs on a conditioned approach-avoidance response in mice. These drugs were epinephrine and nor-epinephrine.

The purpose of this thesis is to determine what effects a dietary magnesium deficiency has on the discriminative avoidance behavior of rats. Three experiments were conducted. Experiment I compared two groups to determine the effects of magnesium deficiency on bar-press discriminative avoidance behavior. The results of Experiment l clearly illustrated that rats fed n diet deficient in magnesium began to lose their discriminative avoidance behavior after approximately five days with a steady decrease in performance over the remaining five days. Experiment II used a single subject design in an attempt to replicate Experiment I and to determine whether or not the magnesium deficiency effect could be reversed. Blood samples of serum magnesium for each rat were taken daily. The results confirmed Experiment I. A magnesium deficiency did cause a decrease in the performance of discriminative bar-press avoidance. Two of the four rats responded to the rehabilitation treatment with a corresponding increase in avoidance behavior with an increase in serum magnesium levels. The other two rats did not recover avoidance performance with rehabilitation, but did improve with regard to other behavioral measurements. Experiment III again employed two groups of rats in an attempt to determine the effects of a magnesium deficiency upon acquisition of a discriminative shuttle box avoidance performance. A pilot study to Experiment III showed a clear effect with normal controls displaying statistically more avoidance responses than the experimentals who received subnormal levels of magnesium. The results from Experiment III however showed no statistically significant difference between the controls and experimentals even though there was a statistical difference in serum magnesium concentration.

Mangroves and seagrass beds serve as essential fish habitat for many economically- and ecologically-valuable species. Depending on their location, these shallow-water habitats are often characterized by substantial fluctuation in salinity levels, which can represent a source of osmoregulatory stress for associated organisms. In South Florida, one of the most important fish species that utilizes these habitats is the gray snapper (Lutjanus griseus). Although this species constitutes a significant portion of the region?s total recreational fishery harvest, the effects of salinity on its distribution, physiology and behavior remain poorly understood. The main goal of this thesis was then to investigate the ecophysiological basis of habitat selection by the gray snapper. Specific objectives include to: (1) examine patterns of distribution and abundance across gradients in environmental salinity; (2) measure physiological status and responses to controlled salinity challenges and; (3) conduct behavioral trials to examine for salinity preferenda (if any). To begin investigating if salinity could be a primary factor structuring the gray snapper assemblages, I examined empirical data collected from Biscayne Bay to test the null hypothesis that gray snapper abundances were evenly distributed along the full salinity range at which samples have been collected. Using the delta approach, three abundance metrics (frequency of occurrence, concentration and delta density) were used as an index for the distribution and abundance of this species. Results indicated that abundance patterns for the smaller gray snapper were consistent with a strategy of reducing osmoregulatory costs by selecting intermediate salinities. However, corresponding abundance patterns for subadult gray snapper were inconsistent with this strategy of minimizing energetic costs, suggesting that this life stage may be indifferent to the range of salinities at which they were observed. These patterns helped developed further hypotheses regarding the ecophysiology of juvenile and subadult gray snapper, the latter of which was then tested via laboratory experiments. Subsequently, I challenged fish in the laboratory with six different salinity treatments (0, 5, 30, 50, 60 and 70ppt, including control) for 192 consecutive hours and collected blood samples at different time points. Results indicated that physiological stress to salinity changes is unlikely to occur at a salinity range of 5 to 50 ppt. At salinities of 0 and 60 ppt transient significant changes in plasma osmolality and/or blood haematocrit were observed, but were corrected after an initial adjustment period of approximately 96 hours. At the highest salinity treatment (70 ppt), a constant osmolality could not be maintained, resulting in death for all fish within 48 hours of exposure. Overall, these findings demonstrate the strong euryhalinity and extraordinary tolerance of this species to both extreme hypo- and hypersaline environments. Finally, I investigated the salinity preference and effects on swimming behavior of the gray snapper in an automated salinity choice shuttlebox via 48-hr trials. In general, gray snapper tested displayed either one of two distinctively different salinity preferences. Half of gray snappers displayed a salinity preference in the range of 9-15 ppt, whereas the other half displayed a salinity preference in the range of 19-23 ppt. Recorded swimming speeds in all fish tested reflected a significant, but weak negative linear relationship with salinity during both time periods of the day (light and dark); however, gray snapper were usually most active during the dark period across all salinities. Overall, these findings reveal that gray snapper prefer slightly hyperosmotic salinities that may minimize the physiological costs of osmoregulation compared to extreme salinities.

No description available.

The principle of competitive exclusion holds that the strongest competitor for a single resource can exclude other species. Yet in many systems, more similar species appear to stably coexist than the small number of limiting resources. Understanding how and when similar species can stably coexist has taken on new urgency in managing biological invasions and their ecological impacts. Recent theoretical advances emphasize the importance of predators in determining coexistence. The effects of predators, however, can be mediated by behavioral changes induced in their prey as well as by their lethality. In this dissertation, I ask how considering multiple trophic levels changes our understanding of how a grass invasion (Pennisetum ciliare) affects species diversity and dynamics in southeastern Arizona. In considering interactions with plant consumers, and with the predators of those consumers, this research reveals more general ecological processes that determine species diversity across biological communities. I first present evidence from a grass removal experiment in the field that shows increased emergence and short-term survival of native perennial plants without grass. This is consistent with Pennisetum ciliare causing the observed concurrent decline in native plant abundance following invasion. I then present results from greenhouse and field studies consistent with that suppression of native plants being driven primarily through resource competition rather than increased rodent granivory. Granivorous rodents do not solely function as consumers, however, because they cache their harvested seeds in shallow scatter-hoards, from which seeds can germinate. Rodents thus act also as seed dispersers in a context-dependent mutualism. The primary granivores in areas invaded by Pennisetum ciliare are pocket mice (genus Chaetodipus), which have a well-studied tendency to concentrate their activity under plant cover to avoid predation by owls. Because the dense canopy of the grass may provide safer refuge, I hypothesized the pocket mice may be directly dispersing native seeds closer to the base of the invasive grass. Such a behavior could increase the competitive effect of the grass on native plant species, further driving the impacts of the invasion. By offering experimental seeds dusted in fluorescent powder and tracking where the seeds were cached, I show that rodents do preferentially cache experimental seeds under the grass. This dispersal interaction may be more general to plant interactions with seed-caching rodents across semi-arid regions that are experiencing plant invasions. Finally, I ask how the predator avoidance behavior exhibited by these rodents affects their ability to coexist with one another. Not only could their diversity affect that of the plant community, but the effects of plant invasions can cascade through other trophic levels. Theoretical understanding of how similar predator avoidance strategy alters coexistence had not yet been developed, however. Instead of a field study, therefore, I modified a general consumer-resource model with three trophic levels to ask whether avoidance behavior by the middle trophic level alters the ability of those species to coexist. I found that more effective avoidance behavior, or greater safety for less cost, increased the importance of resource partitioning in determining overall niche overlap. Lowering niche overlap between two species promotes their coexistence in the sense that their average fitness can be more different and still permit coexistence. These results provide novel understanding of behavioral modifications to population dynamics in multi-trophic coexistence theory applicable to this invasion and more broadly.

The present research explored the role of RES manipulation on ongoing Sidman avoidance behavior. Results of the first phase revealed that both experimental drugs significantly altered RES levels in predicted directions after the first measure; however, only stimulated subjects maintained significant differences after 5 days. No activity-level differences were noted in any subjects due to drugs across time. Sidman avoidance data indicated that RES-stimulated subjects showed significant deterioration in avoidance performance as compared to other groups for the first session. Stimulated subjects were also poorer on the second and third sessions, but statistical significance was not obtained because some saline subjects also showed poorer performance. A rank order correlation revealed that a significant negative correlation existed between RES stimulation and avoidance performance, based on changes in RES levels from baseline to the end of the shock program. These data suggest that increased stress resistance due to RES stimulation may reduce the aversive properties of the shock program, thus decreasing motivation for responding. It was concluded that artificial methods of inducing stress resistance by RES stimulation may be a useful therapeutic technique for patients experiencing psychological stress.

Intertidal habitats are characterized by dynamic, tidally-driven fluctuations in abiotic and biotic factors. Many of the environmental stressors that vary across the intertidal (e.g., temperature, oxygen, food availability, predation pressure) are strong drivers of metabolic rate in ectotherms. As such, we predicted that there may be pronounced differences in the metabolic and stress physiology of conspecific sessile invertebrates occupying at different relative tidal heights. The common acorn barnacle Balanus glandula represents an ideal model organism in which to investigate the possibility of tidal height-dependent physiological differences, owing to their wide distribution in the intertidal zone and their eurytolerant nature. In the first chapter of my thesis, we investigate the hypothesis that B. glandula anchored in the low intertidal have a greater capacity for anaerobic metabolism than conspecifics in the high intertidal, and that this is due to increased predation pressure during submersion. Further, we explore the temporal and spatial fidelity of certain tidal-height driven trends in lactate dehydrogenase activity previously observed in our lab (i.e., higher LDH activity in low intertidal barnacles; Horn et al., 2021), and attempt to identify environmental variables that drive plasticity in LDH activity. We found that, in general, there were higher densities of B. glandula and gastropod whelk predators in the low intertidal compared to the high intertidal, but follow-up studies in the lab revealed that opercular closure in B. glandula was induced by predator exposure (Acanthinucella spirata) for less than 24h. This time frame for shell closure is unlikely to result in internal hypoxia or enhance capacity for anaerobic metabolism. We were therefore not surprised to find that LDH activity in B. glandula was likewise not affected by predator exposures (48h) carried out in the lab. After failing to find an effect of predators on LDH activity in B. glandula, we attempted to replicate the previous finding that LDH activity was highest in low intertidal populations of B. glandula. We did this at the original location in San Luis Obispo Bay, CA as well as at three novel field sites and across seasons and years. While we did observe variation in LDH activity over time and between sites, we did not consistently observe the same trend in LDH activity whereby low intertidal barnacles had the highest activity. In response to these variable patterns, we attempted to identify what environmental parameters, other than predation, might be responsible for plasticity in LDH activity. Unfortunately, neither temperature nor emersion stress – the two variables we examined – had any significant an effect on LDH activity in B. glandula. These data suggest that there must be multiple, interacting stressors – including tidal position - that influence the anaerobic metabolic capacity of B. glandula. In the second chapter of my thesis, we went on to investigate how the response to thermal stress might differ between populations of B. glandula from different vertical heights in the intertidal zone. To this end, we assessed how aerial temperature stress affected oxygen consumption rates (MO2), superoxide dismutase (SOD) activity, and time to mortality in B. glandula collected from both low and high intertidal positions. We found that barnacles from the low intertidal showed a significant increase in MO2 with higher temperature, while MO2 was unaffected by temperature in B. glandula from the high intertidal. We also observed that SOD activity levels were higher in the high intertidal barnacles compared to the low intertidal barnacles, although neither group was increasing SOD activity under higher temperature. Finally, we observed significantly longer survival times during thermal stress in barnacles from the high intertidal zone (e.g., LT50 = 8.75 h vs 5 h at 33˚C for the high and low barnacles, respectively), although this advantage seemed to be lost with the addition of desiccation stress at these same temperatures. It is evident that life in highest reaches of the intertidal zones is physiologically challenging, and this has resulted in a population of B, glandula barnacles that are less sensitive to and better suited to tolerate temperature extremes than conspecifics in the lowest intertidal regions. Understanding how habitat variation may differentially impact the metabolic and thermal stress physiology of B. glandula is increasingly important as climate change progresses. This is particularly significant considering that organisms in the intertidal already reside within a relatively stressful environment and may be living closer to their thermal tolerance limits than animals from less extreme habitats.

Hypoxia refers to any condition in which the water is less than fully saturated with oxygen. Although it is generally accepted that adults are more tolerant of hypoxic conditions than larval stages, there is little information to support this assumption. To determine whether reduced concentrations of dissolved oxygen (DO) affect fishes differently during various early life stages, I examined the responses of two species of fish (fathead minnows (Pimephales promelas) and rainbow trout (Oncorhynchus mykiss)) exposed to low dissolved oxygen concentrations at different ages during the first 100 days post-hatch. The changes in oxygen requirements and respiratory patterns that occur during ontogeny and exposure to hypoxia were observed. The results of this study suggest that the early larval stages appear to be at least as tolerant of short-term exposure to low dissolved oxygen concentrations as the older, more developed stages. Fathead minnows underwent a gradual transition from being metabolic conformers to regulators during development. Hemoglobin appeared to be playing a larger role in oxygen supply in the early post-hatch trout than in the minnows. Fathead minnow larvae produced relatively low concentrations of lactate upon exposure to hypoxia. Conversely, rainbow trout larvae exhibited significant increases in lactate concentration under similar conditions. This implies that there is a threshold oxygen concentration below which trout larvae utilize anaerobic metabolism to provide additional energy. Lactate dehydrogenase activity increased as the rainbow trout larvae aged, suggesting that they develop an anaerobic capacity which could be used to provide additional energy during hypoxia. The minnows did not exhibit this increase in activity. The ability of larval fishes to detect and avoid hypoxic conditions was also examined. The overall trends suggest that throughout this period of development, both fish species gradually leave an area as the dissolved oxygen concentration decline. Both species appeared to leave the hypoxic areas with deliberate motions, indicating that a directed sensor system allowed them to detect oxygen gradients. The results suggest that a combination of physiological, biochemical, and behavioral mechanisms may allow fishes to cope with hypoxia. / Ph. D.

Bakgrund: Prostatacancer är den vanligaste formen av cancer bland män i Sverige. Att få en cancerdiagnos innebär stress för både patienten och dess partner.Syfte: Syftet med denna studie var att undersöka grad av stressreaktioner i form av undvikande beteende och påträngande tankar hos patienter med prostatacancer och deras partner. Ytterligare ett syfte var att kvalitetssäkra mätinstrumentets formulering och svarsalternativ.Metod: Arbetet var en kvantitativ tvärsnittsstudie. Datainsamlingen gjordes med hjälp av enkät “Impact of Event Scale” (IES) samt en enkät utformad enligt “The Question and Answer Model” (QAM) för att kvalitetssäkra IES. Enkäterna delades ut på kirurg- och onkologmottagningar i Uppsala och Falun under våren 2013. Studiens inklusionskriterier var att de patienter som tillfrågades vid ankomst till mottagningarna hade prostatacancer och en partner (n = 34). 12 respondenter deltog. Data analyserades deskriptivt och med Mann- Whitey-test.Resultat: Det visade sig att patienter (n = 6) med prostatacancer har måttlig grad av stressreaktioner i form av undvikande beteende. Partner (n = 6) till patienter med prostatacancer visade sig ha kraftig grad av stressreaktioner i form av påträngande tankar. Det gick inte att se någon signifikant skillnad mellan patienter och partners grad av stress. Majoriteten av deltagarna i studien (n = 11) var säkra på sina svar i enkät IES.Slutsats: Partners tycks ha en högre grad av stress än patienter med prostatacancer, vilket går i linje med liknande studiers resultat. Interventioner för att nå denna grupp och se till dess behov är önskvärt. Fler studier fodras. Det övergripande resultatet tyder på att mätinstrumentet IES är av god kvalitet och mäter det som är avsett att mätas. / Background: Prostate cancer is the most common form of cancer among men in Sweden.Getting a cancer diagnosis is stressful for both the patient and their partner.Objective: The aim of this study was to investigate the degree of stress responses in the form of avoidance behavior and intrusive thoughts in patients with prostate cancer and their partners. Another purpose was to assure the quality and design of the measuring instrument and its response options.Method: The study had a quantitative cross-sectional design. The data was collected using the questionnaire "Impact of Event Scale" (IES) and by a questionnaire framed according to "The Question and Answer Model" (QAM) to safeguard the quality of the IES. Questionnaires were distributed at surgical and oncology clinics in Uppsala and Falun during the spring of 2013. Inclusion criteria for the study were patients with prostate cancer and their partners (n = 34). 12 respondents participated. The data were analyzed descriptively and with Mann-Whitey test.Results: It was found that patients (n = 6) with prostate cancer have moderate degree of stress responses in the form of avoidance behavior. Partners (n = 6) were found to have strong degree of stress responses in the form of intrusive thoughts. Any significant difference between patients and partners dit not appear. The majority of study participants (n = 11) were confident of their answers in the IES.Conclusion: Partners seem to have a higher level of stress than patients with prostate cancer, which is in line with similar studies' results. Interventions to reach this group and ensure its need is desirable. More studies are lined. The overall results indicate that the measuring instrument IES is of good quality and are measuring the concepts that it indicate to measure.