Made available in DSpace on 2016-04-28T20:15:34Z (GMT). No. of bitstreams: 1 2009 - Ana Paula de Aragao Gama.pdf: 3298309 bytes, checksum: 4431d39927607e7468491c92864e1b38 (MD5) Previous issue date: 2009-02-18 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior / This study had as a purpose determine the clinic-pathological and laboratory changes in inoculated sheeps with the Bothrops jararaca and B. jararacussu poison, for the purpose to provide subsidies for the establishment of the diagnosis and differential diagnosis of snake bites. The liofilized venoms were diluted in 1 ml saline and administrated subcutaneously into four sheep. Three of the animals died, and the one that received 0. 5mg/kg (B. jararaca) recovered. Clinical signs were observed from 7 minutes to 1 hours after inoculation. The evolution period varied from 7 hours and 9 minutes up to 21 hours and 59 minutes. The symptoms, independent of the dosage, were swelling (hemorrhage/oedema) of the inoculation site, increased bleeding time and capillary filling; tachycardia, dyspnea, hipocorated mucous membranes and diminished reaction to external stimuli in some animals. Laboratory exam revealed pronounced normocytic and normochromic anemia, trombocytopenia, slight reduction of fibrogen and total plasmatic protein, diminished hematocrit in too animals, besides pronounced increase of the creatinaquinase and lactic dehydrogenase. The main findings at necropsy were large hemorrhagic or oedema areas in the subcutaneous tissue of the inoculation site. Besides hemorrhages/oedema, the main histopathologic alteration was hyalinization of muscle fibers and vessels, most severe at the inoculation site. This study it was seen, that the swelling at the inoculation site and adjacency was due to blood, for B. jararaca venom and oedema, for B. jararacussu venom. / Esse estudo teve como objetivo determinar as altera??es cl?nico-patol?gicas e laboratoriais em ovinos inoculados com a pe?onha de Bothrops jararaca e B. jararacussu, no intuito de fornecer subs?dios que possam facilitar o estabelecimento do diagn?stico e do diagn?stico diferencial dessa condi??o. Os venenos liofilizados foram dilu?dos em 1 ml de solu??o fisiol?gica e administrados a quatro ovinos por via subcut?nea. Tr?s ovinos foram a ?bito e um que recebeu a dose de 0,5 mg/kg (B. jararaca), recuperou-se. Os sinais cl?nicos tiveram in?cio entre 7 minutos e 1 horas. O per?odo de evolu??o variou de 7 horas e 9 minutos a 21 horas e 59 minutos. O quadro cl?nico, independentemente das doses, caracterizou-se por aumento de volume (hematoma/edema) no local da inocula??o, tempo de sangramento e de preenchimento capilar aumentados; taquicardia, dispn?ia, mucosas hipocoradas e apatia em alguns animais. O exame laboratorial revelou acentuada anemia normoc?tica normocr?mica, trombocitopenia, acentuada redu??o de fibrinog?nio e prote?nas plasm?ticas totais, hemat?crito diminu?do em dois animais, al?m de acentuado aumento de creatinaquinase e desidrogenase l?tica em todos os animais. ? necropsia, os principais achados eram extensos hematomas/edemas no local da inocula??o e tecidos adjacentes e, em dois animais, acentuado edema pulmonar agudo (B. jararacussu). Al?m de hemorragias/edema, a principal altera??o histopatol?gica verificada foi necrose das fibras musculares e de vasos, no local de inocula??o e adjac?ncias. Nos ovinos deste estudo, o aumento de volume observado no local de inocula??o e adjac?ncias era constitu?do por sangue (B. jararaca) e edema (B. jararacussu).